pJB866
$157.00
In stock
SKU
PL81496
100μg liquid plasmid(Instantly):$178
| name | pJB866 | another name | apJB866 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Tet | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Pm | Replicon | RK2 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATCTTCGAA TGCATCGCGC GCACCGTACG TCTCGAGGAA TTCCTGCAGG ATATCTGGAT CCACGAAGCT TCCCATGGTG ACGTCACCGG TTCTAGATAC CTAGGTGAGC TCTGGTACCG CGGCCGCAAT TCACATGTTC ATGACTCCAT TATTATTGTT TCTGTTGCAT AAAGCCTAAG GGGTAGGCCT TTCTAGAGAT AGCCATTTTT TGCACTCCTG TATCCGCTTC TTGCAAGGCT GGACTTATCC CTATCAAACC GGACACCCGG TCGTTGGTCA GATCAAGCTA ATTCCCATGC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGT AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCAGATC CCCTTTATCC GCCAATTCGT CGCCGCATGC CCCGACAGCA CGAACTTCTG GTGTTCTCGC TTCTTAAAAA GAACGTCTTC GTTCTGCTTG GCGTTATTTT TGCTTGGAAA AGTGGTCACT GATTGCAAAA AGGATGGCGC AACGTGGCAA TGGGGGTAAC CCGTATACGC ATCACGTCGA GATGCATTTT CATCGACTTG GCGCCTTTCT ACATCACACC AAGCAGCCCA CATTAAAATA AGAGAACCGT GAACTATGGA TTTTTGCTTA TTGAACGAGA AAAGTCAGAT CTTCGTCCAC GCCGAGCCCT ATGCAGTCTC CGATTATGTT AACCAGTATG TCGGTACGCA CTCTATTCGC CTGCCCAAGG GCGGGCCCCC GGCAGGCAGG CTGCACCACA GAATCTTCGG ATGCCTCGAC CTGTGTCGAA TCAGCTACGG CGGTAGCGTG AGGGTAATCT CGCCTGGATT AGAGACCTGT TATCATCTGC AAATAATACT CAAAGGCCAT TGCCTGTGGC GTGGCCATGG CCAGGAGCAC TATTTTGCGC CGGGCGAACT ATTGCTGCTC AATCCGGATG ACCAAGCCGA CCTGACCTAT TCAGAAGATT GCGAGAAATT TATCGTTAAA TTGCCCTCAG TGGTCCTTGA TCGGGCATGC AGTGACAACA ATTGGCACAA GCCGAGGGAG GGTATCCGTT TCGCCGCGCG ACACAATCTC CAGCAACTCG ATGGCTTTAT CAATCTACTC GGGTTAGTTT GTGACGAAGC GGAACATACA AAGTCGATGC CTCGGGTCCA AGAGCACTAT GCGGGGATCA TCGCTTCCAA GCTGCTCGAA ATGCTGGGCA GCAATGTCAG CCGTGAAATT TTCAGCAAAG GTAACCCGTC TTTCGAGCGA GTCGTTCAAT TCATTGAGGA GAATCTCAAA CGGAATATCA GCCTTGAGCG GTTAGCGGAG CTGGCGATGA TGAGTCCACG CTCGCTCTAC AATTTGTTCG AGAAGCATGC CGGCACCACG CCGAAGAACT ACATCCGCAA CCGCAAGCTC GAAAGCATCC GCGCCTGCTT GAACGATCCC AGTGCCAATG TGCGTAGTAT AACTGAGATA GCCCTAGACT ACGGCTTCTT ACATTTGGGA CGCTTCGCTG AAAACTATAG GAGCGCGTTC GGCGAGTTGC CTTCCGACAC CCTGCGTCAA TGCAAAAAGG AAGTGGCTTG ATTACGAACG TAGCCGAAGA AGGGATGGGT TGGCATCGCC CGGCTTTCTT AGACACTCTC CAAGCTCTGA AATAGCGTTT TACAAACTCC ACTGGCTATA GTGCCGGCGT TTGCCCGCGC TCTCCGTGGC CGCGTCCCAA TGCAGGTCGG GTTCCTCGAC CCGAAGTGCC CTTGGGCATC TCCACTTCAG CGTCTGACCT TGCTGGCCAG TTCGCCGCCG ATGCCGAAGT GGCCCTTGGC CATCTCCGTG ATAATCACTC GCACGCTGGT CAGCGGCGCA TCCAGGGAGC GCGAGATGGC CTCGCTGACT TCCCGAATGA GGGTTTCCTT CTGCTCGTCG CTGCGGCCTT CAAGGATGTG GATCTGCTGG CCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGAGCTTA CGGCCAGCCT CGCAGAGCAG GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGATCCGT CGGCTGCAGC GATCCGTCGA TCTATCTCAT CTGCGCAAGG CAGAACGTGA AGACGGCCGC CCTGGACCTC GCCCGCGAGC GCCAGCGCAC GAGGCCGGCG CGCGGACCCG CCGCGGCCCA CGAGCGGACG CCGCAGCAGG AGCGCCAGAA GGCCGCCAGA GAGGCCGAGC GCGGCGTGAG GCTTGGACGC TAGGGCAGGG CATGAAAAAG CCCGTAGCGG GCGCTACGGG GCTCTGACGC GGTGGAAAGG GGGAGGGGAT GTTGTCTACA TGGCTCTGCT GTAGTGAGTG GGTTGCGCTC CGGCAGCGGT CCTGATCAAT CGTCACCCTT TCTCGGTCCT TCAACGTTCC TGACAACGAG CCTCCTTTTC GCCAATCCAT CGACAATCAC CGCGAGTCCC TGCTCGAACG CTGCGTCCGG ACCGGCTTCG TCGAAGGCGT CTATCGCGGC CCGCAACAGC GGCGAGAGCG GAGCCTGTTC AACGGTGCCG CCGCGCTCGC CGGACTCGCT GTCGCCGGCC TGCTCCTCAA GCACGGCCCC AACAGTGAAG TAGCTGATTG TCATCAGCGC ATTGACGGCG TCCCCGGCCG AAAAACCCGC CTCGCAGAGG AAGCGAAGCT GCGCGTCGGC CGTTTCCATC TGCGGTGCGC CCGGTCGCGT GCCGGCATGG ATGCGCGCGC CATCGCGGTA GGCGAGCAGC GCCTGCCTGA AGCTGCGGGC ATTCCCAGTC AGAAATGAGC GCCAGTCGTC GTCGGCTCTC GGCACCGAAG TGCTATGATT CTCCGCCAGC ATGGCTTCGG CCAGTGCGTC GAGCAGCGCC CGCTTGTTCC TGAAGTGCCA GTAAAGCGCC GGCTGCTGAA CCCCCAACCG TTCCGCCAGT TTGCGTGTCG TCAGACCGTC TACGCCGACC TCGTTCAACA GGTCTAGGGC GGCACGGATC ACTGTATTCG GCTGCAACTT TGTCATGCTT GACACTTTAT CACTGATAAA CATAATATGT CCACCAACTT ATCAGTGATA AAGAATCCGC GCGTTCAATC GGACCAGCGG AGGCTGGTCC GGAGGCCAGA CGTGAAACCC AACATACCCC TGATCGTAAT TCTGAGCACT GTCGCGCTCG ACGCTGTCGG CATCGGCCTG ATTATGCCGG TGCTGCCGGG CCTCCTGCGC GATCTGGTTC ACTCGAACGA CGTCACCGCC CACTATGGCA TTCTGCTGGC GCTGTATGCG TTGGTGCAAT TTGCCTGCGC ACCTGTGCTG GGCGCGCTGT CGGATCGTTT CGGGCGGCGG CCAATCTTGC TCGTCTCGCT GGCCGGCGCC ACTGTCGACT ACGCCATCAT GGCGACAGCG CCTTTCCTTT GGGTTCTCTA TATCGGGCGG ATCGTGGCCG GCATCACCGG GGCGACTGGG GCGGTAGCCG GCGCTTATAT TGCCGATATC ACTGATGGCG ATGAGCGCGC GCGGCACTTC GGCTTCATGA GCGCCTGTTT CGGGTTCGGG ATGGTCGCGG GACCTGTGCT CGGTGGGCTG ATGGGCGGTT TCTCCCCCCA CGCTCCGTTC TTCGCCGCGG CAGCCTTGAA CGGCCTCAAT TTCCTGACGG GCTGTTTCCT TTTGCCGGAG TCGCACAAAG GCGAACGCCG GCCGTTACGC CGGGAGGCTC TCAACCCGCT CAGCTTCGTT CGGTGGGCCC GGGGCATGAC CGTCGTCGCC GCCCTGATGG CGGTCTTCTT CATCATGCAA CTTGTCGGAC AGGTGCCGGC CGCGCTTTGG GTCATTTTCG GCGAGGATCG CTTTCACTGG GACGCGACCA CGATCGGCAT TTCGCTTGCC GCATTTGGCA TTCTGCATTC ACTCGCCCAG GCAATGATCA CCGGCCCTGT AGCCGCCCGG CTCGGCGAAA GGCGGGCACT CATGCTCGGA ATGATTGCCG ACGGCACAGG CTACATCCTG CTTGCCTTCG CGACACGGGG ATGGATGGCG TTCCCGATCA TGGTCCTGCT TGCTTCGGGT GGCATCGGAA TGCCGGCGCT GCAAGCAATG TTGTCCAGGC AGGTGGATGA GGAACGCCAG GGGCAGCTGC AAGGCTCACT GGCGGCGCTC ACCAGCCTGA CCTCGATCGT CGGACCCCTC CTCTTCACGG CGATCTATGC GGCTTCTATA ACAACGTGGA ACGGGTGGGC ATGGATTGCA GGCGCTGCCC TCTACTTGCT CTGCCTGCCG GCGCTGCGTC GCGGGCTTTG GAGCGGCGCA GGGCAACGAG CCGATCGCTG ATCGTGGAAA CGATAGGGAC GGATCGCTGC AGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG GGGGATCAGG ACCGCTGCCG GAGCGCAACC CACTCACTAC AGCAGAGCCA TGTAGGGCCG CCGGCGTTGT GGATACCACG CGGAAAACTT GGCCCTCACT GACAGATGAG GGGCGGACGT TGACACTTGA GGGGCCGACT CACCCGGCGC GGCGTTGACA GATGAGGGGC AGGCTCGATT TCGGCCGGCG ACGTGGAGCT GGCCAGCCTC GCAAATCGGC GAAAACGCCT GATTTTACGC GAGTTTCCCA CAGATGATGT GGACAAGCCT GGGGATAAGT GCCCTGCGGT ATTGACACTT GAGGGGCGCG ACTACTGACA GATGAGGGGC GCGATCCTTG ACACTTGAGG GGCAGAGTGA TGACAGATGA GGGGCGCACC TATTGACATT TGAGGGGCTG TCCACAGGCA GAAAATCCAG CATTTGCAAG GGTTTCCGCC CGTTTTTCGG CCACCGCTAA CCTGTCTTTT AACCTGCTTT TAAACCAATA TTTATAAACC TTGTTTTTAA CCAGGGCTGC GCCCTGGCGC GTGACCGCGC ACGCCGAAGG GGGGTGCCCC CCCTTCTCGA ACCCTCCCGG CCCGCTAACG CGGCACCCCA TCCCCCCAGG GGCTGCGCCC CTCGGCCGCG AACGACCTCA CCCCAAAAAT GGCAGCCACG TAGAAAGCCA GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCGACG GATCGATCCG GGGAATTAAT TCCGGGGCAA TCCCGCAAGG AGGGTGAATG AATCGGACGT TTGACCGGAA GGCATACAGG CAAGAACTGA TCGACGCGGG GTTTTCCGCC GAGGATGCCG AAACCATCGC AAGCCGCACC GTCATGCGTG CGCCCCGCGA AACCTTCCAG TCCGTCGGCT CGATGGTCCA GCAAGCTACG GCCAAGATCG AGCGCGACAG CGTGCAACTG GCTCCCCCTG CCCTGCCCGC GCCATCGGCC GCCGTGGAGC GTTCGCGTCG TCTCGAACAG GAGGCGGCAG GTTTGGCGAA GTCGATGACC ATCGACACGC GAGGAACTAT GACGACCAAG AAGCGAAAAA CCGCCGGCGA GGACCTGGCA AAACAGGTCA GCGAGGCCAA GCAGGCCGCG TTGCTGAAAC ACACGAAGCA GCAGATCAAG GAAATGCAGC TTTCCTTGTT CGATATTGCG CCGTGGCCGG ACACGATGCG AGCGATGCCA AACGACACGG CCCGCTCTGC CCTGTTCACC ACGCGCAACA AGAAAATCCC GCGCGAGGCG CTGCAAAACA AGGTCATTTT CCACGTCAAC AAGGACGTGA AGATCACCTA CACCGGCGTC GAGCTGCGGG CCGACGATGA CGAACTGGTG TGGCAGCAGG TGTTGGAGTA CGCGAAGCGC ACCCCTATCG GCGAGCCGAT CACCTTCACG TTCTACGAGC TTTGCCAGGA CCTGGGCTGG TCGATCAATG GCCGGTATTA CACGAAGGCC GAGGAATGCC TGTCGCGCCT ACAGGCGACG GCGATGGGCT TCACGTCCGA CCGCGTTGGG CACCTGGAAT CGGTGTCGCT GCTGCACCGC TTCCGCGTCC TGGACCGTGG CAAGAAAACG TCCCGTTGCC AGGTCCTGAT CGACGAGGAA ATCGTCGTGC TGTTTGCTGG CGACCACTAC ACGAAATTCA TATGGGAGAA GTACCGCAAG CTGTCGCCGA CGGCCCGACG GATGTTCGAC TATTTCAGCT CGCACCGGGA GCCGTACCCG CTCAAGCTGG AAACCTTCCG CCTCATGTGC GGATCGGATT CCACCCGCGT GAAGAAGTGG CGCGAGCAGG TCGGCGAAGC CTGCGAAGAG TTGCGAGGCA GCGGCCTGGT GGAACACGCC TGGGTCAATG ATGACCTGGT GCATTGCAAA CGCTAGGGCC TTGTGGGGTC AGTTCCGGCT GGGGGTTCAG CAGCCACCTG CATCGCAAGC TAGCTTGCTA GAGGGTCAGC TTTATGCTTG TAAACCGTTT TGTGAAAAAA TTTTTAAAAT AAAAAAGGGG ACCTCTAGGG TCCCCAATTA ATTAGTAATA TAATCTATTA AAGGTCATTC AAAAGGTCAT CCACCGGATC AATTCCCCTG CTCGCGCAGG CTGGGTGCCA AGCTCTCGGG TAACATCAAG GCCCGATCCT TGGTAGTCGG CAAATAATGT CTAACAATTC GTTCAAGCCG ACGCCGCTTC GCGGCGCGGC TTAACTCAAG CGTTAGATGC ACTAAGCACA TAATTGCTCA CAGCCAAACT ATCAGGTCAA GTCTGCTTTT ATTATTTTTA AGCGTGCATA ATAAGCCCTA CACAAATTGG GAGATATATC ATGAAAGGCT GGCTTTTTCT TGTTATCGCA ATAGTTGGCG AAGTAATCGC AACATCCGCA TTAAAATCTA GCGAGGGCTT TACTAAGCTG ATCCGGTGGA TGACCTTTTG AATGACCTTT AATAGATTAT ATTACTAATT AATTGGGGAC CCTAGAGGTC CCCTTTTTTA TTTTAAAAAT TTTTTCACAA AACGGTTTAC AAGCATAAAG CTGACTCTAG CTAGAG
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