pCIG-CNGA1-FLAG
$113.00
In stock
SKU
PL81486
CNCG;CNG1;RP49;CNCG1;CNG-1;RCNC1;RCNCa;RCNCalpha
100μg liquid plasmid(Instantly):$134
| name | pCIG-CNGA1-FLAG | another name | apCIG-CNGA1-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGNACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGGGTCGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGGGC GCGCGCCAGG CGGGGCGGGG CGGGGCGAGG GGCGGGGCGG GGCGAGGCGG AGAGGTGCGG CGGCAGCCAA TCAGAGCGGC GCGCTCCGAA AGTTTCCTTT TATGGCGAGG CGGCGGCGGC GGCGGCCCTA TAAAAAGCGA AGCGCGCGGC GGGCGGGAGT CGCTGCGTTG CCTTCGCCCC GTGCCCCGCT CCGCGCCGCC TCGCGCCGCC CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTCGT TTCTTTTCTG TGGCTGCGTG AAAGCCTTAA AGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GGAGCGGCTC GGGGGGTGCG TGCGTGTGTG TGTGCGTGGG GAGCGCCGCG TGCGGCCCGC GCTGCCCGGC GGCTGTGAGC GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCGTGTG CGCGAGGGGA GCGCGGCCGG GGGCGGTGCC CCGCGGTGCG GGGGGGCTGC GAGGGGAACA AAGGCTGCGT GCGGGGTGTG TGCGTGGGGG GGTGAGCAGG GGGTGTGGGC GCGGCGGTCG GGCTGTAACC CCCCCCTGCA CCCCCCTCCC CGAGTTGCTG AGCACGGCCC GGCTTCGGGT GCGGGGCTCC GTACGGGGCG TGGCGCGGGG CTCGCCGTGC CGGGCGGGGG GTGGCGGCAG GTGGGGGTGC CGGGCGGGGC GGGGCCGCCT CGGGCCGGGG AGGGCTCGGG GGAGGGGCGC GGCGGCCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC AAATCTGTGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC CCCCTCTAGC GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGCTCGAGAT GAAACTATCC ATGAAGAACA ATATTATCAA TACACAGCAG TCTTTTGTAA CCATGCCCAA TGTGATTGTA CCAGATATTG AAAAGGAAAT ACGAAGGATG GAAAATGGAG CATGCAGCTC CTTTTCTGAG GATGATGACA GTGCCTCTAC ATCTGAAGAA TCAGAGAATG AAAACCCTCA TGCAAGGGGT TCCTTTAGTT ATAAGTCACT CAGAAAGGGA GGACCATCAC AGAGGGAGCA GTACCTGCCT GGTGCCATTG CACTTTTTAA TGTGAACAAC AGCAGCAATA AGGACCAGGA ACCAGAAGAA AAAAAGAAAA AGAAAAAAGA AAAGAAGAGC AAGTCAGATG ATAAAAACGA AAATAAAAAC GACCCAGAGA AGAAAAAGAA GAAAAAGGAC AAAGAGAAGA AAAAGAAAGA GGAGAAAAGC AAAGATAAGA AAGAAGAGGA GAAGAAAGAA GTTGTGGTTA TTGATCCCTC GGGAAACACA TATTACAACT GGCTGTTTTG CATCACATTA CCTGTTATGT ACAACTGGAC AATGGTTATT GCCAGAGCAT GTTTTGATGA ACTTCAATCT GATTACCTAG AATATTGGCT CATTTTGGAT TACGTATCAG ACATAGTCTA TTTAATCGAT ATGTTTGTAC GAACAAGGAC AGGTTACCTA GAACAAGGAC TGCTGGTAAA GGAAGAACTT AAACTCATAA ATAAATATAA ATCCAACTTG CAATTTAAAC TTGATGTTCT GTCACTGATA CCAACTGATT TGCTGTATTT TAAGTTAGGG TGGAACTATC CAGAAATTAG ATTAAACAGG TTGTTACGGT TCTCTCGTAT GTTTGAGTTC TTCCAGAGAA CAGAAACAAG GACAAACTAT CCAAACATCT TCAGGATTTC CAACCTTGTT ATGTATATCG TCATCATTAT CCACTGGAAT GCATGTGTGT TCTACTCTAT TTCTAAAGCT ATTGGATTTG GAAATGATAC ATGGGTCTAC CCTGATATTA ATGATCCTGA ATTTGGCCGT TTGGCTAGAA AATACGTATA CAGCCTTTAC TGGTCTACAC TGACTTTGAC TACCATTGGT GAAACACCCC CTCCCGTGAG GGATTCTGAG TATGTCTTTG TGGTGGTTGA TTTCCTAATT GGAGTGTTAA TTTTTGCTAC CATCGTTGGT AACATAGGTT CTATGATTTC CAACATGAAT GCAGCCAGAG CAGAATTTCA AGCAAGAATT GATGCTATCA AGCAATATAT GCATTTTCGA AATGTAAGCA AAGATATGGA AAAGAGGGTT ATTAAATGGT TTGACTACCT GTGGACCAAC AAAAAAACAG TTGATGAGAA AGAAGTCTTA AAGTATCTAC CTGATAAACT AAGAGCAGAA ATTGCCATCA ACGTTCACTT AGACACATTA AAAAAGGTAC GCATTTTTGC TGATTGTGAA GCTGGTCTGT TGGTGGAGTT GGTCTTGAAA TTGCAACCCC AAGTCTACAG TCCTGGAGAT TATATTTGCA AGAAAGGGGA TATCGGACGA GAGATGTACA TTATCAAGGA AGGCAAACTC GCTGTGGTGG CAGATGATGG AGTCACTCAG TTTGTGGTAT TGAGCGATGG CAGCTACTTC GGTGAGATCA GCATTCTTAA CATTAAAGGG AGCAAAGCTG GCAATCGAAG AACGGCCAAT ATTAAAAGTA TTGGCTACTC AGACCTGTTC TGTCTCTCAA AAGATGACCT CATGGAAGCT CTAACTGAGT ACCCAGATGC CAAAACTATG CTGGAAGAGA AAGGGAAGCA GATTTTAATG AAAGATGGTC TACTGGATCT AAACATTGCA AATGCTGGCA GTGATCCTAA AGATCTTGAA GAGAAGGTTA CTCGAATGGA GGGGTCAGTA GACCTCCTGC AAACCAGGTT TGCCCGAATC TTGGCTGAGT ATGAGTCCAT GCAGCAGAAA CTGAAACAAA GATTAACCAA GGTTGAGAAA TTTCTGAAAC CGCTTATTGA CACAGAATTT TCAAGTATTG AGGGACCTGG AGCGGAAAGT GGGCCCATCG ACTCTACAGA TTACAAGGAT GACGACGATA AGTAGGAATT CCTGCAGCCC GGCCGCTACG TAAATTCCGC CCCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT ACACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGCAAT TCACTCCTCA GGTGCAGGCT GCCTATCAGA AGGTGGTGGC TGGTGTGGCC AATGCCCTGG CTCACAAATA CCACTGAGAT CTTTTTCCCT CTGCCAAAAA TTATGGGGAC ATCATGAAGC CCCTTGAGCA TCTGACTTCT GGCTAATAAA GGAAATTTAT TTTCATTGCA ATAGTGTGTT GGAATTTTTT GTGTCTATCA CTCGGAAGGA CATATGGGAG GGCAAATCAT TTAAAACATC AGAATGAGTA TTTGGTTTAG AGTTTGGCAA CATATGCCCA TATGCTGGCT GCCATGAACA AAGGTTGGCT ATAAAGAGGT CATCAGTATA TGAAACAGCC CCCTGCTGTC CATTCCTTAT TCCATAGAAA AGCCTTGACT TGAGGTTAGA TTTTTTTTAT ATTTTGTTTT GTGTTATTTT TTTCTTTAAC ATCCCTAAAA TTTTCCTTAC ATGTTTTACT AGCCAGATTT TTCCTCCTCT CCTGACTACT CCCAGTCATA GCTGTCCCTC TTCTCTTATG GAGATCCCTC GACCTGCACC GTCGACCAGC TGGTCGACGG TGCACCGTCG ACCAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAG
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