pEarleyGate 205
$99.00
In stock
SKU
PL81476
pEarleyGate205
100μg liquid plasmid(Instantly):$120
| name | pEarleyGate 205 | another name | apEarleyGate 205 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCAGGATA TATTGTGGTG TAAACAAATT GACGCTTAGA CAACTTAATA ACACATTGCG GACGTTTTTA ATGTACTGAA TTAACGCCGA ATTAATTCGA GCTCGGATCT GATAATTTAT TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGAAT TCCAATCCCA CAAAAATCTG AGCTTAACAG CACAGTTGCT CCTCTCAGAG CAGAATCGGG TATTCAACAC CCTCATATCA ACTACTACGT TGTGTATAAC GGTCCACATG CCGGTATATA CGATGACTGG GGTTGTACAA AGGCGGCAAC AAACGGCGTT CCCGGAGTTG CACACAAGAA ATTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TACACAACAA GTCAGCAAAC AGACAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCTAA GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGGCTC ACGCTAGGAA CCAAAAGGCC CAGCAGTGAT CCAGCCCCAA AAGAGATCTC CTTTGCCCCG GAGATTACAA TGGACGATTT CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTCGAGA TCACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CATATTGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC AGCATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTGCC TAGGATGGAA AAGAGAAGAT GGAAAAAGAA TTTCATAGCC GTCTCAGCAG CCAACCGCTT TAAGAAAATC TCATCCTCCG GGGCACTTGA TTATGATATT CCAACTACTG CTAGCGAGAA TTTGTATTTT CAGGGTGAGC TCAAAACCGC GGCTCTTGCG CAACACGATG AAGCCGTGGA CAACAAATTC AACAAAGAAC AACAAAACGC GTTCTATGAG ATCTTACATT TACCTAACTT AAACGAAGAA CAACGAAACG CCTTCATCCA AAGTTTAAAA GATGACCCAA GCCAAAGCGC TAACCTTTTA GCAGAAGCTA AAAAGCTAAA TGATGCTCAG GCGCCGAAAG TAGACAACAA ATTCAACAAA GAACAACAAA ACGCGTTCTA TGAGATCTTA CATTTACCTA ACTTAAACGA AGAACAACGA AACGCCTTCA TCCAAAGTTT AAAAGATGAC CCAAGCCAAA GCGCTAACCT TTTAGCAGAA GCTAAAAAGC TAAATGGTGC TCAGGCGCCG AAAGTAGACG CGAATTCCGC GGGGAAGTCA ACCTGATTAA TTAAGACCCG GGACTAGTCC CTAGAGTCCT GCTTTAATGA GATATGCGAG ACGCCTATGA TCGCATGATA TTTGCTTTCA ATTCTGTTGT GCACGTTGTA AAAAACCTGA GCATGTGTAG CTCAGATCCT TACCGCCGGT TTCGGTTCAT TCTAATGAAT ATATCACCCG TTACTATCGT ATTTTTATGA ATAATATTCT CCGTTCAATT TACTGATTGT ACCCTACTAC TTATATGTAC AATATTAAAA TGAAAACAAT ATATTGTGCT GAATAGGTTT ATAGCGACAT CTATGATAGA GCGCCACAAT AACAAACAAT TGCGTTTTAT TATTACAAAT CCAATTTTAA AAAAAGCGGC AGAACCGGTC AAACCTAAAA GACTGATTAC ATAAATCTTA TTCAAATTTC AAAAGTGCCC CAGGGGCTAG TATCTACGAC ACACCGAGCG GCGAACTAAT AACGCTCACT GAAGGGAACT CCGGTTCCCC GCCGGCGCGC ATGGGTGAGA TTCCTTGAAG TTGAGTATTG GCCGTCCGCT CTACCGAAAG TTACGGGCAC CATTCAACCC GGTCCAGCAC GGCGGCCGGG TAACCGACTT GCTGCCCCGA GAATTATGCA GCATTTTTTT GGTGTATGTG GGCCCCAAAT GAAGTGCAGG TCAAACCTTG ACAGTGACGA CAAATCGTTG GGCGGGTCCA GGGCGAATTT TGCGACAACA TGTCGAGGCT CAGCAGGACC TGCAGGCATG CAAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGCTAG AGCAGCTTGA GCTTGGATCA GATTGTCGTT TCCCGCCTTC AGTTTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA TAACGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC GTCCATTTGT ATGTGCATGC CAACCACAGG GTTCCCCTCG GGATCAAAGT ACTTTGATCC AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA ACGACATGTC GCACAAGTCC TAAGTTACGC GACAGGCTGC CGCCCTGCCC TTTTCCTGGC GTTTTCTTGT CGCGTGTTTT AGTCGCATAA AGTAGAATAC TTGCGACTAG AACCGGAGAC ATTACGCCAT GAACAAGAGC GCCGCCGCTG GCCTGCTGGG CTATGCCCGC GTCAGCACCG ACGACCAGGA CTTGACCAAC CAACGGGCCG AACTGCACGC GGCCGGCTGC ACCAAGCTGT TTTCCGAGAA GATCACCGGC ACCAGGCGCG ACCGCCCGGA GCTGGCCAGG ATGCTTGACC ACCTACGCCC TGGCGACGTT GTGACAGTGA CCAGGCTAGA CCGCCTGGCC CGCAGCACCC GCGACCTACT GGACATTGCC GAGCGCATCC AGGAGGCCGG CGCGGGCCTG CGTAGCCTGG CAGAGCCGTG GGCCGACACC ACCACGCCGG CCGGCCGCAT GGTGTTGACC GTGTTCGCCG GCATTGCCGA GTTCGAGCGT TCCCTAATCA TCGACCGCAC CCGGAGCGGG CGCGAGGCCG CCAAGGCCCG AGGCGTGAAG TTTGGCCCCC GCCCTACCCT CACCCCGGCA CAGATCGCGC ACGCCCGCGA GCTGATCGAC CAGGAAGGCC GCACCGTGAA AGAGGCGGCT GCACTGCTTG GCGTGCATCG CTCGACCCTG TACCGCGCAC TTGAGCGCAG CGAGGAAGTG ACGCCCACCG AGGCCAGGCG GCGCGGTGCC TTCCGTGAGG ACGCATTGAC CGAGGCCGAC GCCCTGGCGG CCGCCGAGAA TGAACGCCAA GAGGAACAAG CATGAAACCG CACCAGGACG GCCAGGACGA ACCGTTTTTC ATTACCGAAG AGATCGAGGC GGAGATGATC GCGGCCGGGT ACGTGTTCGA GCCGCCCGCG CACGTCTCAA CCGTGCGGCT GCATGAAATC CTGGCCGGTT TGTCTGATGC CAAGCTGGCG GCCTGGCCGG CCAGCTTGGC CGCTGAAGAA ACCGAGCGCC GCCGTCTAAA AAGGTGATGT GTATTTGAGT AAAACAGCTT GCGTCATGCG GTCGCTGCGT ATATGATGCG ATGAGTAAAT AAACAAATAC GCAAGGGGAA CGCATGAAGG TTATCGCTGT ACTTAACCAG AAAGGCGGGT CAGGCAAGAC GACCATCGCA ACCCATCTAG CCCGCGCCCT GCAACTCGCC GGGGCCGATG TTCTGTTAGT CGATTCCGAT CCCCAGGGCA GTGCCCGCGA TTGGGCGGCC GTGCGGGAAG ATCAACCGCT AACCGTTGTC GGCATCGACC GCCCGACGAT TGACCGCGAC GTGAAGGCCA TCGGCCGGCG CGACTTCGTA GTGATCGACG GAGCGCCCCA GGCGGCGGAC TTGGCTGTGT CCGCGATCAA GGCAGCCGAC TTCGTGCTGA TTCCGGTGCA GCCAAGCCCT TACGACATAT GGGCCACCGC CGACCTGGTG GAGCTGGTTA AGCAGCGCAT TGAGGTCACG GATGGAAGGC TACAAGCGGC CTTTGTCGTG TCGCGGGCGA TCAAAGGCAC GCGCATCGGC GGTGAGGTTG CCGAGGCGCT GGCCGGGTAC GAGCTGCCCA TTCTTGAGTC CCGTATCACG CAGCGCGTGA GCTACCCAGG CACTGCCGCC GCCGGCACAA CCGTTCTTGA ATCAGAACCC GAGGGCGACG CTGCCCGCGA GGTCCAGGCG CTGGCCGCTG AAATTAAATC AAAACTCATT TGAGTTAATG AGGTAAAGAG AAAATGAGCA AAAGCACAAA CACGCTAAGT GCCGGCCGTC CGAGCGCACG CAGCAGCAAG GCTGCAACGT TGGCCAGCCT GGCAGACACG CCAGCCATGA AGCGGGTCAA CTTTCAGTTG CCGGCGGAGG ATCACACCAA GCTGAAGATG TACGCGGTAC GCCAAGGCAA GACCATTACC GAGCTGCTAT CTGAATACAT CGCGCAGCTA CCAGAGTAAA TGAGCAAATG AATAAATGAG TAGATGAATT TTAGCGGCTA AAGGAGGCGG CATGGAAAAT CAAGAACAAC CAGGCACCGA CGCCGTGGAA TGCCCCATGT GTGGAGGAAC GGGCGGTTGG CCAGGCGTAA GCGGCTGGGT TGTCTGCCGG CCCTGCAATG GCACTGGAAC CCCCAAGCCC GAGGAATCGG CGTGAGCGGT CGCAAACCAT CCGGCCCGGT ACAAATCGGC GCGGCGCTGG GTGATGACCT GGTGGAGAAG TTGAAGGCCG CGCAGGCCGC CCAGCGGCAA CGCATCGAGG CAGAAGCACG CCCCGGTGAA TCGTGGCAAG CGGCCGCTGA TCGAATCCGC AAAGAATCCC GGCAACCGCC GGCAGCCGGT GCGCCGTCGA TTAGGAAGCC GCCCAAGGGC GACGAGCAAC CAGATTTTTT CGTTCCGATG CTCTATGACG TGGGCACCCG CGATAGTCGC AGCATCATGG ACGTGGCCGT TTTCCGTCTG TCGAAGCGTG ACCGACGAGC TGGCGAGGTG ATCCGCTACG AGCTTCCAGA CGGGCACGTA GAGGTTTCCG CAGGGCCGGC CGGCATGGCC AGTGTGTGGG ATTACGACCT GGTACTGATG GCGGTTTCCC ATCTAACCGA ATCCATGAAC CGATACCGGG AAGGGAAGGG AGACAAGCCC GGCCGCGTGT TCCGTCCACA CGTTGCGGAC GTACTCAAGT TCTGCCGGCG AGCCGATGGC GGAAAGCAGA AAGACGACCT GGTAGAAACC TGCATTCGGT TAAACACCAC GCACGTTGCC ATGCAGCGTA CGAAGAAGGC CAAGAACGGC CGCCTGGTGA CGGTATCCGA GGGTGAAGCC TTGATTAGCC GCTACAAGAT CGTAAAGAGC GAAACCGGGC GGCCGGAGTA CATCGAGATC GAGCTAGCTG ATTGGATGTA CCGCGAGATC ACAGAAGGCA AGAACCCGGA CGTGCTGACG GTTCACCCCG ATTACTTTTT GATCGATCCC GGCATCGGCC GTTTTCTCTA CCGCCTGGCA CGCCGCGCCG CAGGCAAGGC AGAAGCCAGA TGGTTGTTCA AGACGATCTA CGAACGCAGT GGCAGCGCCG GAGAGTTCAA GAAGTTCTGT TTCACCGTGC GCAAGCTGAT CGGGTCAAAT GACCTGCCGG AGTACGATTT GAAGGAGGAG GCGGGGCAGG CTGGCCCGAT CCTAGTCATG CGCTACCGCA ACCTGATCGA GGGCGAAGCA TCCGCCGGTT CCTAATGTAC GGAGCAGATG CTAGGGCAAA TTGCCCTAGC AGGGGAAAAA GGTCGAAAAG GTCTCTTTCC TGTGGATAGC ACGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGA ACCCGTACAT TGGGAACCCA AAGCCGTACA TTGGGAACCG GTCACACATG TAAGTGACTG ATATAAAAGA GAAAAAAGGC GATTTTTCCG CCTAAAACTC TTTAAAACTT ATTAAAACTC TTAAAACCCG CCTGGCCTGT GCATAACTGT CTGGCCAGCG CACAGCCGAA GAGCTGCAAA AAGCGCCTAC CCTTCGGTCG CTGCGCTCCC TACGCCCCGC CGCTTCGCGT CGGCCTATCG CGGCCGCTGG CCGCTCAAAA ATGGCTGGCC TACGGCCAGG CAATCTACCA GGGCGCGGAC AAGCCGCGCC GTCGCCACTC GACCGCCGGC GCCCACATCA AGGCACCCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGCA TTCTAGGTAC TAAAACAATT CATCCAGTAA AATATAATAT TTTATTTTCT CCCAATCAGG CTTGATCCCC AGTAAGTCAA AAAATAGCTC GACATACTGT TCTTCCCCGA TATCCTCCCT GATCGACCGG ACGCAGAAGG CAATGTCATA CCACTTGTCC GCCCTGCCGC TTCTCCCAAG ATCAATAAAG CCACTTACTT TGCCATCTTT CACAAAGATG TTGCTGTCTC CCAGGTCGCC GTGGGAAAAG ACAAGTTCCT CTTCGGGCTT TTCCGTCTTT AAAAAATCAT ACAGCTCGCG CGGATCTTTA AATGGAGTGT CTTCTTCCCA GTTTTCGCAA TCCACATCGG CCAGATCGTT ATTCAGTAAG TAATCCAATT CGGCTAAGCG GCTGTCTAAG CTATTCGTAT AGGGACAATC CGATATGTCG ATGGAGTGAA AGAGCCTGAT GCACTCCGCA TACAGCTCGA TAATCTTTTC AGGGCTTTGT TCATCTTCAT ACTCTTCCGA GCAAAGGACG CCATCGGCCT CACTCATGAG CAGATTGCTC CAGCCATCAT GCCGTTCAAA GTGCAGGACC TTTGGAACAG GCAGCTTTCC TTCCAGCCAT AGCATCATGT CCTTTTCCCG TTCCACATCA TAGGTGGTCC CTTTATACCG GCTGTCCGTC ATTTTTAAAT ATAGGTTTTC ATTTTCTCCC ACCAGCTTAT ATACCTTAGC AGGAGACATT CCTTCCGTAT CTTTTACGCA GCGGTATTTT TCGATCAGTT TTTTCAATTC CGGTGATATT CTCATTTTAG CCATTTATTA TTTCCTTCCT CTTTTCTACA GTATTTAAAG ATACCCCAAG AAGCTAATTA TAACAAGACG AACTCCAATT CACTGTTCCT TGCATTCTAA AACCTTAAAT ACCAGAAAAC AGCTTTTTCA AAGTTGTTTT CAAAGTTGGC GTATAACATA GTATCGACGG AGCCGATTTT GAAACCGCGG TGATCACAGG CAGCAACGCT CTGTCATCGT TACAATCAAC ATGCTACCCT CCGCGAGATC ATCCGTGTTT CAAACCCGGC AGCTTAGTTG CCGTTCTTCC GAATAGCATC GGTAACATGA GCAAAGTCTG CCGCCTTACA ACGGCTCTCC CGCTGACGCC GTCCCGGACT GATGGGCTGC CTGTATCGAG TGGTGATTTT GTGCCGAGCT GCCGGTCGGG GAGCTGTTGG CTGGCTGG
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