pEarleyGate 302
$99.00
In stock
SKU
PL81470
pEarleyGate302
100μg liquid plasmid(Instantly):$120
| name | pEarleyGate 302 | another name | apEarleyGate 302 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCTGGCAGGA TATATTGTGG TGTAAACAAA TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG AATTAACGCC GAATTAATTC GAGCTCGGAT CTGATAATTT ATTTGAAAAT TCATAAGAAA AGCAAACGTT ACATGAATTG ATGAAACAAT ACAAAGACAG ATAAAGCCAC GCACATTTAG GATATTGGCC GAGATTACTG AATATTGAGT AAGATCACGG AATTTCTGAC AGGAGCATGT CTTCAATTCA GCCCAAATGG CAGTTGAAAT ACTCAAACCG CCCCATATGC AGGAGCGGAT CATTCATTGT TTGTTTGGTT GCCTTTGCCA ACATGGGAGT CCAAGATTCT GCAGTCAAAT CTCGGTGACG GGCAGGACCG GACGGGGCGG TACCGGCAGG CTGAAGTCCA GCTGCCAGAA ACCCACGTCA TGCCAGTTCC CGTGCTTGAA GCCGGCCGCC CGCAGCATGC CGCGGGGGGC ATATCCGAGC GCCTCGTGCA TGCGCACGCT CGGGTCGTTG GGCAGCCCGA TGACAGCGAC CACGCTCTTG AAGCCCTGTG CCTCCAGGGA CTTCAGCAGG TGGGTGTAGA GCGTGGAGCC CAGTCCCGTC CGCTGGTGGC GGGGGGAGAC GTACACGGTC GACTCGGCCG TCCAGTCGTA GGCGTTGCGT GCCTTCCAGG GGCCCGCGTA GGCGATGCCG GCGACCTCGC CGTCCACCTC GGCGACGAGC CAGGGATAGC GCTCCCGCAG ACGGACGAGG TCGTCCGTCC ACTCCTGCGG TTCCTGCGGC TCGGTACGGA AGTTGACCGT GCTTGTCTCG ATGTAGTGGT TGACGATGGT GCAGACCGCC GGCATGTCCG CCTCGGTGGC ACGGCGGATG TCGGCCGGGC GTCGTTCTGG GCTCATCGAT TCGATTTGGT GTATCGAGAT TGGTTATGAA ATTCAGATGC TAGTGTAATG TATTGGTAAT TTGGGAAGAT ATAATAGGAA GCAAGGCTAT TTATCCATTT CTGAAAAGGC GAAATGGCGT CACCGCGAGC GTCACGCGCA TTCCGTTCTT GCTGTAAAGC GTTGTTTGGT ACACTTTTGA CTAGCGAGGC TTGGCGTGTC AGCGTATCTA TTCAAAAGTC GTTAATGGCT GCGGATCAAG AAAAAGTTGG AATAGAAACA GAATACCCGC GAAATTCAGG CCCGGTTGCC ATGTCCTACA CGCCGAAATA AACGACCAAA TTAGTAGAAA AATAAAAACT GACTCGGATA CTTACGTCAC GTCTTGCGCA CTGATTTGAA AAATCTCAGA ATTGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCA TCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCATATTGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT ATTGAGAATA TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT TTGTGATGGC TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG GCAGGGCGGG GCGTAAACGC GTGGATCCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGATATG TTGTGTTTTA CAGCATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTGG ACTACAAAGA CGATGACGAC AAATGACTAG GTGAGTCTAG AGAGTTAATT AAGACCCGGG ACTAGTCCCT AGAGTCCTGC TTTAATGAGA TATGCGAGAC GCCTATGATC GCATGATATT TGCTTTCAAT TCTGTTGTGC ACGTTGTAAA AAACCTGAGC ATGTGTAGCT CAGATCCTTA CCGCCGGTTT CGGTTCATTC TAATGAATAT ATCACCCGTT ACTATCGTAT TTTTATGAAT AATATTCTCC GTTCAATTTA CTGATTGTAC CCTACTACTT ATATGTACAA TATTAAAATG AAAACAATAT ATTGTGCTGA ATAGGTTTAT AGCGACATCT ATGATAGAGC GCCACAATAA CAAACAATTG CGTTTTATTA TTACAAATCC AATTTTAAAA AAAGCGGCAG AACCGGTCAA ACCTAAAAGA CTGATTACAT AAATCTTATT CAAATTTCAA AAGTGCCCCA GGGGCTAGTA TCTACGACAC ACCGAGCGGC GAACTAATAA CGCTCACTGA AGGGAACTCC GGTTCCCCGC CGGCGCGCAT GGGTGAGATT CCTTGAAGTT GAGTATTGGC CGTCCGCTCT ACCGAAAGTT ACGGGCACCA TTCAACCCGG TCCAGCACGG CGGCCGGGTA ACCGACTTGC TGCCCCGAGA ATTATGCAGC ATTTTTTTGG TGTATGTGGG CCCCAAATGA AGTGCAGGTC AAACCTTGAC AGTGACGACA AATCGTTGGG CGGGTCCAGG GCGAATTTTG CGACAACATG TCGAGGCTCA GCAGGACCTG CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGCTAGAG CAGCTTGAGC TTGGATCAGA TTGTCGTTTC CCGCCTTCAG TTTAAACTAT CAGTGTTTGA CAGGATATAT TGGCGGGTAA ACCTAAGAGA AAAGAGCGTT TATTAGAATA ACGGATATTT AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT GTGCATGCCA ACCACAGGGT TCCCCTCGGG ATCAAAGTAC TTTGATCCAA CCCCTCCGCT GCTATAGTGC AGTCGGCTTC TGACGTTCAG TGCAGCCGTC TTCTGAAAAC GACATGTCGC ACAAGTCCTA AGTTACGCGA CAGGCTGCCG CCCTGCCCTT TTCCTGGCGT TTTCTTGTCG CGTGTTTTAG TCGCATAAAG TAGAATACTT GCGACTAGAA CCGGAGACAT TACGCCATGA ACAAGAGCGC CGCCGCTGGC CTGCTGGGCT ATGCCCGCGT CAGCACCGAC GACCAGGACT TGACCAACCA ACGGGCCGAA CTGCACGCGG CCGGCTGCAC CAAGCTGTTT TCCGAGAAGA TCACCGGCAC CAGGCGCGAC CGCCCGGAGC TGGCCAGGAT GCTTGACCAC CTACGCCCTG GCGACGTTGT GACAGTGACC AGGCTAGACC GCCTGGCCCG CAGCACCCGC GACCTACTGG ACATTGCCGA GCGCATCCAG GAGGCCGGCG CGGGCCTGCG TAGCCTGGCA GAGCCGTGGG CCGACACCAC CACGCCGGCC GGCCGCATGG TGTTGACCGT GTTCGCCGGC ATTGCCGAGT TCGAGCGTTC CCTAATCATC GACCGCACCC GGAGCGGGCG CGAGGCCGCC AAGGCCCGAG GCGTGAAGTT TGGCCCCCGC CCTACCCTCA CCCCGGCACA GATCGCGCAC GCCCGCGAGC TGATCGACCA GGAAGGCCGC ACCGTGAAAG AGGCGGCTGC ACTGCTTGGC GTGCATCGCT CGACCCTGTA CCGCGCACTT GAGCGCAGCG AGGAAGTGAC GCCCACCGAG GCCAGGCGGC GCGGTGCCTT CCGTGAGGAC GCATTGACCG AGGCCGACGC CCTGGCGGCC GCCGAGAATG AACGCCAAGA GGAACAAGCA TGAAACCGCA CCAGGACGGC CAGGACGAAC CGTTTTTCAT TACCGAAGAG ATCGAGGCGG AGATGATCGC GGCCGGGTAC GTGTTCGAGC CGCCCGCGCA CGTCTCAACC GTGCGGCTGC ATGAAATCCT GGCCGGTTTG TCTGATGCCA AGCTGGCGGC CTGGCCGGCC AGCTTGGCCG CTGAAGAAAC CGAGCGCCGC CGTCTAAAAA GGTGATGTGT ATTTGAGTAA AACAGCTTGC GTCATGCGGT CGCTGCGTAT ATGATGCGAT GAGTAAATAA ACAAATACGC AAGGGGAACG CATGAAGGTT ATCGCTGTAC TTAACCAGAA AGGCGGGTCA GGCAAGACGA CCATCGCAAC CCATCTAGCC CGCGCCCTGC AACTCGCCGG GGCCGATGTT CTGTTAGTCG ATTCCGATCC CCAGGGCAGT GCCCGCGATT GGGCGGCCGT GCGGGAAGAT CAACCGCTAA CCGTTGTCGG CATCGACCGC CCGACGATTG ACCGCGACGT GAAGGCCATC GGCCGGCGCG ACTTCGTAGT GATCGACGGA GCGCCCCAGG CGGCGGACTT GGCTGTGTCC GCGATCAAGG CAGCCGACTT CGTGCTGATT CCGGTGCAGC CAAGCCCTTA CGACATATGG GCCACCGCCG ACCTGGTGGA GCTGGTTAAG CAGCGCATTG AGGTCACGGA TGGAAGGCTA CAAGCGGCCT TTGTCGTGTC GCGGGCGATC AAAGGCACGC GCATCGGCGG TGAGGTTGCC GAGGCGCTGG CCGGGTACGA GCTGCCCATT CTTGAGTCCC GTATCACGCA GCGCGTGAGC TACCCAGGCA CTGCCGCCGC CGGCACAACC GTTCTTGAAT CAGAACCCGA GGGCGACGCT GCCCGCGAGG TCCAGGCGCT GGCCGCTGAA ATTAAATCAA AACTCATTTG AGTTAATGAG GTAAAGAGAA AATGAGCAAA AGCACAAACA CGCTAAGTGC CGGCCGTCCG AGCGCACGCA GCAGCAAGGC TGCAACGTTG GCCAGCCTGG CAGACACGCC AGCCATGAAG CGGGTCAACT TTCAGTTGCC GGCGGAGGAT CACACCAAGC TGAAGATGTA CGCGGTACGC CAAGGCAAGA CCATTACCGA GCTGCTATCT GAATACATCG CGCAGCTACC AGAGTAAATG AGCAAATGAA TAAATGAGTA GATGAATTTT AGCGGCTAAA GGAGGCGGCA TGGAAAATCA AGAACAACCA GGCACCGACG CCGTGGAATG CCCCATGTGT GGAGGAACGG GCGGTTGGCC AGGCGTAAGC GGCTGGGTTG TCTGCCGGCC CTGCAATGGC ACTGGAACCC CCAAGCCCGA GGAATCGGCG TGAGCGGTCG CAAACCATCC GGCCCGGTAC AAATCGGCGC GGCGCTGGGT GATGACCTGG TGGAGAAGTT GAAGGCCGCG CAGGCCGCCC AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC GTGGCAAGCG GCCGCTGATC GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC GCCGTCGATT AGGAAGCCGC CCAAGGGCGA CGAGCAACCA GATTTTTTCG TTCCGATGCT CTATGACGTG GGCACCCGCG ATAGTCGCAG CATCATGGAC GTGGCCGTTT TCCGTCTGTC GAAGCGTGAC CGACGAGCTG GCGAGGTGAT CCGCTACGAG CTTCCAGACG GGCACGTAGA GGTTTCCGCA GGGCCGGCCG GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC GGTTTCCCAT CTAACCGAAT CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG CCGCGTGTTC CGTCCACACG TTGCGGACGT ACTCAAGTTC TGCCGGCGAG CCGATGGCGG AAAGCAGAAA GACGACCTGG TAGAAACCTG CATTCGGTTA AACACCACGC ACGTTGCCAT GCAGCGTACG AAGAAGGCCA AGAACGGCCG CCTGGTGACG GTATCCGAGG GTGAAGCCTT GATTAGCCGC TACAAGATCG TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA GCTAGCTGAT TGGATGTACC GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT TCACCCCGAT TACTTTTTGA TCGATCCCGG CATCGGCCGT TTTCTCTACC GCCTGGCACG CCGCGCCGCA GGCAAGGCAG AAGCCAGATG GTTGTTCAAG ACGATCTACG AACGCAGTGG CAGCGCCGGA GAGTTCAAGA AGTTCTGTTT CACCGTGCGC AAGCTGATCG GGTCAAATGA CCTGCCGGAG TACGATTTGA AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG CTACCGCAAC CTGATCGAGG GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT AGGGCAAATT GCCCTAGCAG GGGAAAAAGG TCGAAAAGGT CTCTTTCCTG TGGATAGCAC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGAAC CCGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGT CACACATGTA AGTGACTGAT ATAAAAGAGA AAAAAGGCGA TTTTTCCGCC TAAAACTCTT TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC ATAACTGTCT GGCCAGCGCA CAGCCGAAGA GCTGCAAAAA GCGCCTACCC TTCGGTCGCT GCGCTCCCTA CGCCCCGCCG CTTCGCGTCG GCCTATCGCG GCCGCTGGCC GCTCAAAAAT GGCTGGCCTA CGGCCAGGCA ATCTACCAGG GCGCGGACAA GCCGCGCCGT CGCCACTCGA CCGCCGGCGC CCACATCAAG GCACCCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGCATT CTAGGTACTA AAACAATTCA TCCAGTAAAA TATAATATTT TATTTTCTCC CAATCAGGCT TGATCCCCAG TAAGTCAAAA AATAGCTCGA CATACTGTTC TTCCCCGATA TCCTCCCTGA TCGACCGGAC GCAGAAGGCA ATGTCATACC ACTTGTCCGC CCTGCCGCTT CTCCCAAGAT CAATAAAGCC ACTTACTTTG CCATCTTTCA CAAAGATGTT GCTGTCTCCC AGGTCGCCGT GGGAAAAGAC AAGTTCCTCT TCGGGCTTTT CCGTCTTTAA AAAATCATAC AGCTCGCGCG GATCTTTAAA TGGAGTGTCT TCTTCCCAGT TTTCGCAATC CACATCGGCC AGATCGTTAT TCAGTAAGTA ATCCAATTCG GCTAAGCGGC TGTCTAAGCT ATTCGTATAG GGACAATCCG ATATGTCGAT GGAGTGAAAG AGCCTGATGC ACTCCGCATA CAGCTCGATA ATCTTTTCAG GGCTTTGTTC ATCTTCATAC TCTTCCGAGC AAAGGACGCC ATCGGCCTCA CTCATGAGCA GATTGCTCCA GCCATCATGC CGTTCAAAGT GCAGGACCTT TGGAACAGGC AGCTTTCCTT CCAGCCATAG CATCATGTCC TTTTCCCGTT CCACATCATA GGTGGTCCCT TTATACCGGC TGTCCGTCAT TTTTAAATAT AGGTTTTCAT TTTCTCCCAC CAGCTTATAT ACCTTAGCAG GAGACATTCC TTCCGTATCT TTTACGCAGC GGTATTTTTC GATCAGTTTT TTCAATTCCG GTGATATTCT CATTTTAGCC ATTTATTATT TCCTTCCTCT TTTCTACAGT ATTTAAAGAT ACCCCAAGAA GCTAATTATA ACAAGACGAA CTCCAATTCA CTGTTCCTTG CATTCTAAAA CCTTAAATAC CAGAAAACAG CTTTTTCAAA GTTGTTTTCA AAGTTGGCGT ATAACATAGT ATCGACGGAG CCGATTTTGA AACCGCGGTG ATCACAGGCA GCAACGCTCT GTCATCGTTA CAATCAACAT GCTACCCTCC GCGAGATCAT CCGTGTTTCA AACCCGGCAG CTTAGTTGCC GTTCTTCCGA ATAGCATCGG TAACATGAGC AAAGTCTGCC GCCTTACAAC GGCTCTCCCG CTGACGCCGT CCCGGACTGA TGGGCTGCCT GTATCGAGTG GTGATTTTGT GCCGAGCTGC CGGTCGGGGA GCTGTTGGCT GGCTGG
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