pLSLR
$99.00
In stock
SKU
PL81463
100μg liquid plasmid(Instantly):$120
| name | pLSLR | another name | apLSLR |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAAACCATCC GGCCCGGTAC AAATCGGCGC GGCGCTGGGT GATGACCTGG TGGAGAAGTT GAAGGCCGCG CAGGCCGCCC AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC GTGGCAAGCG GCCGCTGATC GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC GCCGTCGATT AGGAAGCCGC CCAAGGGCGA CGAGCAACCA GATTTTTTCG TTCCGATGCT CTATGACGTG GGCACCCGCG ATAGTCGCAG CATCATGGAC GTGGCCGTTT TCCGTCTGTC GAAGCGTGAC CGACGAGCTG GCGAGGTGAT CCGCTACGAG CTTCCAGACG GGCACGTAGA GGTTTCCGCA GGGCCGGCCG GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC GGTTTCCCAT CTAACCGAAT CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG CCGCGTGTTC CGTCCACACG TTGCGGACGT ACTCAAGTTC TGCCGGCGAG CCGATGGCGG AAAGCAGAAA GACGACCTGG TAGAAACCTG CATTCGGTTA AACACCACGC ACGTTGCCAT GCAGCGTACG AAGAAGGCCA AGAACGGCCG CCTGGTGACG GTATCCGAGG GTGAAGCCTT GATTAGCCGC TACAAGATCG TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA GCTAGCTGAT TGGATGTACC GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT TCACCCCGAT TACTTTTTGA TCGATCCCGG CATCGGCCGT TTTCTCTACC GCCTGGCACG CCGCGCCGCA GGCAAGGCAG AAGCCAGATG GTTGTTCAAG ACGATCTACG AACGCAGTGG CAGCGCCGGA GAGTTCAAGA AGTTCTGTTT CACCGTGCGC AAGCTGATCG GGTCAAATGA CCTGCCGGAG TACGATTTGA AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG CTACCGCAAC CTGATCGAGG GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT AGGGCAAATT GCCCTAGCAG GGGAAAAAGG TCGAAAAGGC CTCTTTCCTG TGGATAGCAC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGAAC CCGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGT CACACATGTA AGTGACTGAT ATAAAAGAGA AAAAAGGCGA TTTTTCCGCC TAAAACTCTT TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC ATAACTGTCT GGCCAGCGCA CAGCCGAAGA GCTGCAAAAA GCGCCTACCC TTCGGTCGCT GCGCTCCCTA CGCCCCGCCG CTTCGCGTCG GCCTATCGCG GCCGCTGGCC GCTCAAAAAT GGCTGGCCTA CGGCCAGGCA ATCTACCAGG GCGCGGACAA GCCGCGCCGT CGCCACTCGA CCGCCGGCGC CCACATCAAG GCACCCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAAACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGCATT CTAGGTACTA AAACAATTCA TCCAGTAAAA TATAATATTT TATTTTCTCC CAATCAGGCT TGATCCCCAG TAAGTCAAAA AATAGCTCGA CATACTGTTC TTCCCCGATA TCCTCCCTGA TCGACCGGAC GCAGAAGGCA ATGTCATACC ACTTGTCCGC CCTGCCGCTT CTCCCAAGAT CAATAAAGCC ACTTACTTTG CCATCTTTCA CAAAGATGTT GCTGTCTCCC AGGTCGCCGT GGGAAAAGAC AAGTTCCTCT TCGGGCTTTT CCGTCTTTAA AAAATCATAC AGCTCGCGCG GATCTTTAAA TGGAGTGTCT TCTTCCCAGT TTTCGCAATC CACATCGGCC AGATCGTTAT TCAGTAAGTA ATCCAATTCG GCTAAGCGGC TGTCTAAGCT ATTCGTATAG GGACAATCCG ATATGTCGAT GGAGTGAAAG AGCCTGATGC ACTCCGCATA CAGCTCGATA ATCTTTTCAG GGCTTTGTTC ATCTTCATAC TCTTCCGAGC AAAGGACGCC ATCGGCCTCA CTCATGAGCA GATTGCTCCA GCCATCATGC CGTTCAAAGT GCAGGACCTT TGGAACAGGC AGCTTTCCTT CCAGCCATAG CATCATGTCC TTTTCCCGTT CCACATCATA GGTGGTCCCT TTATACCGGC TGTCCGTCAT TTTTAAATAT AGGTTTTCAT TTTCTCCCAC CAGCTTATAT ACCTTAGCAG GAGACATTCC TTCCGTATCT TTTACGCAGC GGTATTTTTC GATCAGTTTT TTCAATTCCG GTGATATTCT CATTTTAGCC ATTTATTATT TCCTTCCTCT TTTCTACAGT ATTTAAAGAT ACCCCAAGAA GCTAATTATA ACAAGACGAA CTCCAATTCA CTGTTCCTTG CATTCTAAAA CCTTAAATAC CAGAAAACAG CTTTTTCAAA GTTGTTTTCA AAGTTGGCGT ATAACATAGT ATCGACGGAG CCGATTTTGA AACCGCGGTG ATCACAGGCA GCAACGCTCT GTCATCGTTA CAATCAACAT GCTACCCTCC GCGAGATCAT CCGTGTTTCA AACCCGGCAG CTTAGTTGCC GTTCTTCCGA ATAGCATCGG TAACATGAGC AAAGTCTGCC GCCTTACAAC GGCTCTCCCG CTGACGCCGT CCCGGACTGA TGGGCTGCCT GTATCGAGTG GTGATTTTGT GCCGAGCTGC CGGTCGGGGA GCTGTTGGCT GGCTGGTGGC AGGATATATT GTGGTGTAAA CAAATTGACG CTTAGACAAC TTAATAACAC ATTGCGGACG TTTTTAATGT ACTGAATTAA CGCCGAATTA ATTCGGGGGA TCTGGATTTT AGTACTGGAT TTTGGTTTTA GGAATTAGAA ATTTTATTGA TAGAAGTATT TTACAAATAC AAATACATAC TAAGGGTTTC TTATATGCTC AACACATGAG CGAAACCCTA TAGGAACCCT AATTCCCTTA TCTGGGAACT ACTCACACAT TATTATGGAG AAAATAGAGC TTGTCGATCG TTAATTAATC ACCTCCAAGA TCCCGGACGA GTGCTGGGGC GTCGGTTTCC ACTATCGGCG AGTACTTCTA CACAGCCATC GGTCCAGACG GCCGCGCTTC TGCGGGCGAT TTGTGTACGC CCGACAGTCC CGGCTCCGGA TCGGACGATT GCGTCGCATC GACCCTGCGC CCAAGCTGCA TCATCGAAAT TGCCGTCAAC CAAGCTCTGA TAGAGTTGGT CAAGACCAAT GCGGAGCATA TACGCCCGGA GCCGCGGCGA TCCTGCAAGC TCCGGATGCC TCCGCTCGAA GTAGCGCGTC TGCTGCTCCA TACAAGCCAA CCACGGCCTC CAGAAGAAGA TGTTGGCGAC CTCGTATTGG GAATCCCCGA ACATCGCCTC GCTCCAGTCA ATGACCGCTG TTATGCGGCC ATTGTCCGTC AGGACATTGT TGGAGCCGAA ATCCGCGTGC ACGAGGTGCC GGACTTCGGG GCAGTCCTCG GCCCAAAGCA TCAGCTCATC GAGAGCCTGC GCGACGGACG CACTGACGGT GTCGTCCATC ACAGTTTGCC AGTGATACAC ATGGGGATCA GCAATCGCGC ATATGAAATC ACGCCATGTA GTGTATTGAC CGATTCCTTG CGGTCCGAAT GGGCCGAACC CGCTCGTCTG GCTAAGATCG GCCGCAGCGA TCGCATCCAT GGCCTCCGCG ACCGGCTGCA GAACAGCGGG CAGTTCGGTT TCAGGCAGGT CTTGCAACGT GACACCCTGT GCACGGCGGG AGATGCAATA GGTCAGGCTC TCGCTGAATT CCCCAATGTC AAGCACTTCC GGAATCGGGA GCGCGGCCGA TGCAAAGTGC CGATAAACAT AACGATCTTT GTAGAAACCA TCGGCGCAGC TATTTACCCG CAGGACATAT CCACGCCCTC CTACATCGAA GCTGAAAGCA CGAGATTCTT CGCCCTCCGA GAGCTGCATC AGGTCGGACA CGCTGTCGAA CTTTTCGATC AGAAACTTCT CGACAGACGT CGCGGTGAGT TCAGGCTTTT TCATCCTAGG AGAGATAGAT TTGTAGAGAG AGACTGGTGA TTTCAGCGTG TCCTCTCCAA ATGAAATGAA CTTCCTTATA TAGAGGAAGG TCTTGCGAAG GATAGTGGGA TTGTGCGTCA TCCCTTACGT CAGTGGAGAT ATCACATCAA TCCACTTGCT TTGAAGACGT GGTTGGAACG TCTTCTTTTT CCACGATGCT CCTCGTGGGT GGGGGTCCAT CTTTGGGACC ACTGTCGGCA GAGGCATCTT GAACGATAGC CTTTCCTTTA TCGCAATGAT GGCATTTGTA GGTGCCACCT TCCTTTTCTA CTGTCCTTTT GATGAAGTGA CAGATAGCTG GGCAATGGAA TCCGAGGAGG TTTCCCGATA TTACCCTTTG TTGAAAAGTC TCAATAGCCC TTTGGTCTTC TGAGACTGTA TCTTTGATAT TCTTGGAGTA GACGAGAGTG TCGTGCTCCA CCATGTTATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTGAA CGATAGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGT GCCACCTTCC TTTTCTACTG TCCTTTTGAT GAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCCGATATTA CCCTTTGTTG AAAAGTCTCA ATAGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTCT TGGAGTAGAC GAGAGTGTCG TGCTCCACCA TGTCGACTAT CGGACGAGCT CAAAGTTTAA CGCGTTAGCA GAAGGCATGT TGTTGTGACT CCGAGGGGTT GCCTCAAACT CTATCTTATA ACCGGCGTGG AGGCATGGAG GCAGGGGTAT TTTGGTCATT TTAATAGATA GTGGAAAATG ACGTGGAATT TACTTAAAGA CGAAGTCTTT GCGACAAGGG GGGGCCCACG CCGAATTTAA TATTACCGGC GTGGCCCCCC CTTATCGCGA GTGCTTTAGC ACGAGCGGTC CAGATTTAAA GTAGAAAATT TCCCGCCCAC TAGGGTTAAA GGTGTTCACA CTATAAAAGC ATATACGATG TGATGGTATT TGATGGAGCG TATATTGTAT CAGGTATTTC CGTTGGATAC GAATTATTCG TACGACCCTC GGCGCGCCGA GAACTTCCAG AGCTTTGGGT ACGTCACGTG GCTCGAGCGC GTAGTCCTCG GTAATATCGC AGAACAAAAG TACCTGATAT CGAGTGTACT TCAAGTCAGT GGGAAATCAA TAAAATGATT ATTTTATGAA TATATTTCAT TGTGCAAGTA GATAGAAATT ACATATGTTA CATAACACAC GAAATAAACA AAAAAAGACA ATCCAAAAAC AAACACCCCA AAAAAAATAA TCACTTTAGA TAAACTCGTA TGAGGAGAGG CACGTTCAGT GACTCGACGA TTCCCGAGCA AAAAAAGTCT CCCCGTCACA CATGTAGTGG GTGACGCAAT TATCTTTAAA GTAATCCTTC TGTTGACTTG TCATTGATAA CATCCAGTCT TCGTCAGGAT TGCAAAGAAT TATAGAAGGG ATCCCACCTT TTATTTTCTT CTTTTTTCCA TATTTAGGGT TGACAGTGAA ATCAGACTGG CAACCTATTA ATTGCTTCCA CAATGGGACG AACTTGAAGG GGATGTCGTC GATGATATTA TAGGTGGCGT GTTCATCGTA GTTGGTGAAA TCGATGGTAC CGTTCCAATA GTTGTGTCGT CCGAGACTTC TAGCCCAGGT GGTCTTTCCG GTACGAGTTG GTCCGCAGAT GTAGAGGCTG GGGTGTCGGA TTCCATTCCT TCCATTGTCC TTGTTAAATC GGCCATCCAT TCAAGGTCAG ATTGAGCTTG TTGGTATGAG ACAGGATGTA TGTAAGTATA AGCGTCTATG CTTACATGGT ATAGATGGGT TTCCCTCCAG GAGTGTAGAT CTTCGTGGCA GCGAAGATCT GATTCTGTGA AGGGCGACAC ATACGGTTCA GGTTGTGGAG GGAATAATTT GTTGGCTGAA TATTCCAGCC ATTGAAGCTT TGTTGCCCAT TCATGAGGGA ATTCTTCCTT GATCATGTCA AGATATTCCT CCTTAGACGT TGCAGTCTGG ATAATAGTTC TCCATCGTGC GTCAGATTTG CGAGGAGAAA CCTTATGATC TCGGAAATCT CCTCTGGTTT TAATATCTCC GTCCTTTGAT ATGTAATCAA GGACTTGTTT AGAGTTTCTA GCTGGCTGGA TATTAGGGTG ATTTCCTTCA AAATCGAAAA AAGAAGGATC CCTAATACAA GGTTTTTTAT CAAGCTGGAG AAGAGCATGA TAGTGGGTAG TGCCATCTTG ATGAAGCTCA GAAGCAACAC CAAGGAAGAA AATAAGAAAA GGTGTGAGTT TCTCCCAGAG AAACTGGAAT AAATCATCTC TTTGAGATGA GCACTTGGGA TAGGTAAGGA AAACATATTT AGATTGGAGT CTGAAGTTCT TACTAGCAGA AGGCATGTTG TTGTGACTCC GAGGGGTTGC CTCAAACTCT ATCTTATAAC CGGCGTGGAG GCATGGAGGC AGGGGTATTT TGGTCATTTT AATAGATAGT GGAAAATGAC GTGGAATTTA CTTAAAGACG AAGTCTTTGC GACAAGGGGG GGCCCACGCC GAATTTAATA TTACCGGCGT GGCCCCCCCT TATCGCGAGT GCTTTAGCAC GAGCGGTCCA GATTTAAAGT AGAAAATTTC CCGCCCACTA GGGTTAAAGG TGTTCACACT ATAAAAGCAT ATACGATGTG ATGGTATTTG ATGGAGCGTA TATTGTATCA GGTATTTCCG TTGGATACGA ATTATTCGTA CGACCCTCAT AGTTTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA TAACGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC GTCCATTTGT ATGTGCATGC CAACCACAGG GTTCCCCTCG GGATCAAAGT ACTTTGATCC AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA ACGACATGTC GCACAAGTCC TAAGTTACGC GACAGGCTGC CGCCCTGCCC TTTTCCTGGC GTTTTCTTGT CGCGTGTTTT AGTCGCATAA AGTAGAATAC TTGCGACTAG AACCGGAGAC ATTACGCCAT GAACAAGAGC GCCGCCGCTG GCCTGCTGGG CTATGCCCGC GTCAGCACCG ACGACCAGGA CTTGACCAAC CAACGGGCCG AACTGCACGC GGCCGGCTGC ACCAAGCTGT TTTCCGAGAA GATCACCGGC ACCAGGCGCG ACCGCCCGGA GCTGGCCAGG ATGCTTGACC ACCTACGCCC TGGCGACGTT GTGACAGTGA CCAGGCTAGA CCGCCTGGCC CGCAGCACCC GCGACCTACT GGACATTGCC GAGCGCATCC AGGAGGCCGG CGCGGGCCTG CGTAGCCTGG CAGAGCCGTG GGCCGACACC ACCACGCCGG CCGGCCGCAT GGTGTTGACC GTGTTCGCCG GCATTGCCGA GTTCGAGCGT TCCCTAATCA TCGACCGCAC CCGGAGCGGG CGCGAGGCCG CCAAGGCCCG AGGCGTGAAG TTTGGCCCCC GCCCTACCCT CACCCCGGCA CAGATCGCGC ACGCCCGCGA GCTGATCGAC CAGGAAGGCC GCACCGTGAA AGAGGCGGCT GCACTGCTTG GCGTGCATCG CTCGACCCTG TACCGCGCAC TTGAGCGCAG CGAGGAAGTG ACGCCCACCG AGGCCAGGCG GCGCGGTGCC TTCCGTGAGG ACGCATTGAC CGAGGCCGAC GCCCTGGCGG CCGCCGAGAA TGAACGCCAA GAGGAACAAG CATGAAACCG CACCAGGACG GCCAGGACGA ACCGTTTTTC ATTACCGAAG AGATCGAGGC GGAGATGATC GCGGCCGGGT ACGTGTTCGA GCCGCCCGCG CACGGCTCAA CCGTGCGGCT GCATGAAATC CTGGCCGGTT TGTCTGATGC CAAGCTGGCG GCCTGGCCGG CCAGCTTGGC CGCTGAAGAA ACCGAGCGCC GCCGTCTAAA AAGGTGATGT GTATTTGAGT AAAACAGCTT GCGTCATGCG GTCGCTGCGT ATATGATGCG ATGAGTAAAT AAACAAATAC GCAAGGGGAA CGCATGAAGG TTATCGCTGT ACTTAACCAG AAAGGCGGGT CAGGCAAGAC GACCATCGCA ACCCATCTAG CCCGCGCCCT GCAACTCGCC GGGGCCGATG TTCTGTTAGT CGATTCCGAT CCCCAGGGCA GTGCCCGCGA TTGGGCGGCC GTGCGGGAAG ATCAACCGCT AACCGTTGTC GGCATCGACC GCCCGACGAT TGACCGCGAC GTGAAGGCCA TCGGCCGGCG CGACTTCGTA GTGATCGACG GAGCGCCCCA GGCGGCGGAC TTGGCTGTGT CCGCGATCAA GGCAGCCGAC TTCGTGCTGA TTCCGGTGCA GCCAAGCCCT TACGACATAT GGGCCACCGC CGACCTGGTG GAGCTGGTTA AGCAGCGCAT TGAGGTCACG GATGGAAGGC TACAAGCGGC CTTTGTCGTG TCGCGGGCGA TCAAAGGCAC GCGCATCGGC GGTGAGGTTG CCGAGGCGCT GGCCGGGTAC GAGCTGCCCA TTCTTGAGTC CCGTATCACG CAGCGCGTGA GCTACCCAGG CACTGCCGCC GCCGGCACAA CCGTTCTTGA ATCAGAACCC GAGGGCGACG CTGCCCGCGA GGTCCAGGCG CTGGCCGCTG AAATTAAATC AAAACTCATT TGAGTTAATG AGGTAAAGAG AAAATGAGCA AAAGCACAAA CACGCTAAGT GCCGGCCGTC CGAGCGCACG CAGCAGCAAG GCTGCAACGT TGGCCAGCCT GGCAGACACG CCAGCCATGA AGCGGGTCAA CTTTCAGTTG CCGGCGGAGG ATCACACCAA GCTGAAGATG TACGCGGTAC GCCAAGGCAA GACCATTACC GAGCTGCTAT CTGAATACAT CGCGCAGCTA CCAGAGTAAA TGAGCAAATG AATAAATGAG TAGATGAATT TTAGCGGCTA AAGGAGGCGG CATGGAAAAT CAAGAACAAC CAGGCACCGA CGCCGTGGAA TGCCCCATGT GTGGAGGAAC GGGCGGTTGG CCAGGCGTAA GCGGCTGGGT TGTCTGCCGG CCCTGCAATG GCACTGGAAC CCCCAAGCCC GAGGAATCGG CGTGACGGTC G
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