pCMV-SPORT6-GAPDH
$70.00
In stock
SKU
PL81402
NM_002046.6;G3PD;GAPD;HEL-S-162eP
100μg liquid plasmid(Instantly):$91
| name | pCMV-SPORT6-GAPDH | another name | apCMV-SPORT6-GAPDH |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-SPORT6 | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCAGTGCCTA GCTTATAATA CGACTCACTA TAGGGACCAC TTTGTACAAG AAAGCTGGGT ACGCGTAAGC TTGGGCCCCT CGAGGGATAC TCTAGAGCGG CCGCCCTTTT TTTTTTTTTT TTTTTGGTTG AGCACAGGGT ACTTTATTGA TGGTACATGA CAAGGTGCGG CTCCCTAGGC CCCTCCCCTC TTCAAGGGGT CTACATGGCA ACTGTGAGGA GGGGAGATTC AGTGTGGTGG GGGACTGAGT GTGGCAGGGA CTCCCCAGCA GTGAGGGTCT CTCTCTTCCT CTTGTGCTCT TGCTGGGGCT GGTGGTCCAG GGGTCTTACT CCTTGGAGGC CATGTGGGCC ATGAGGTCCA CCACCCTGTT GCTGTAGCCA AATTCGTTGT CATACCAGGA AATGAGCTTG ACAAAGTGGT CGTTGAGGGC AATGCCAGCC CCAGCGTCAA AGGTGGAGGA GTGGGTGTCG CTGTTGAAGT CAGAGGAGAC CACCTGGTGC TCAGTGTAGC CCAGGATGCC CTTGAGGGGG CCCTCCGACG CCTGCTTCAC CACCTTCTTG ATGTCATCAT ATTTGGCAGG TTTTTCTAGA CGGCAGGTCA GGTCCACCAC TGACACGTTG GCAGTGGGGA CACGGAAGGC CATGCCAGTG AGCTTCCCGT TCAGCTCAGG GATGACCTTG CCCACAGCCT TGGCAGCGCC AGTAGAGGCA GGGATGATGT TCTGGAGAGC CCCGCGGCCA TCACGCCACA GTTTCCCGGA GGGGCCATCC ACAGTCTTCT GGGTGGCAGT GATGGCATGG ACTGTGGTCA TGAGTCCTTC CACGATACCA AAGTTGTCAT GGATGACCTT GGCCAGGGGT GCTAAGCAGT TGGTGGTGCA GGAGGCATTG CTGATGATCT TGAGGCTGTT GTCATACTTC TCATGGTTCA CACCCATGAC GAACATGGGG GCATCAGCAG AGGGGGCAGA GATGATGACC CTTTTGGCTC CCCCCTGCAA ATGAGCCCCA GCCTTCTCCA TGGTGGTGAA GACGCCAGTG GACTCCACGA CGTACTCAGC GCCAGCATCG CCCCACTTGA TTTTGGAGGG ATCTCGCTCC TGGAAGATGG TGATGGGATT TCCATTGATG ACAAGCTTCC CGTTCTCAGC CTTGACGGTG CCATGGAATT TGCCATGGGT GGAATCATAT TGGAACATGT AAACCATGTA GTTGAGGTCA ATGAAGGGGT CATTGATGGC AACAATATCC ACTTTACCAG AGTTAAAAGC AGCCCTGGTG ACCAGGCGCC CAATACGACC AAATCCGTTG ACTCCGACCT TCACCTTCCC CATGGTGTCT GAGCGATGTG GCTCGGCTGG CGACGCAAAA GAAGATGCGG CTGACTGTCG AACACGGACG CGTGGGTCGA CGATATCCCG GGAATTCCGG ACCGGTACCA GCCTGCTTTT TTGTACAAAC TTGTTCTATA GTGTCACCTA AATAGGCCTA ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC CGCGGCCTAG GCTAGAGTCC GGAGGCTGGA TCGGTCCCGG TGTCTTCTAT GGAGGTCAAA ACAGCGTGGA TGGCGTCTCC AGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGACATGCA TCTAATGAGT GAAAGGGCCT CGTACTACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAC GCGCGAATTG CAAGCTCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGCCAT AACTTCGTAT AGCATACATT ATACGAAGTT ATGGCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGCCAG GGGTGGGCAC ACATATTTGA TACCAGCGAT CCCTACACAG CACATAATTC AATGCGACTT CCCTCTATCG CACATCTTAG ACCTTTATTC TCCCTCCAGC ACACATCGAA GCTGCCGAGC AAGCCGTTCT CACCAGTCCA AGACCTGGCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAAATTGTAA ACGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGGA TCGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCATGAACA GACTGTGAGG ACTGAGGGGC CTGAAATGAG CCTTGGGACT GTGAATCTAA AATACACAAA CAATTAGAAT CACTAGCTCC TGTGTATAAT ATTTTCATAA ATCATACTCA GTAAGCAAAA CTCTCAAGCA GCAAGCATAT GCAGCTAGTT TAACACATTA TACACTTAAA AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA TTATGTCACA CCACAGAAGT AAGGTTCCTT CACAAAGATC CCAAGCTAGC AGTTTTCCCA GTCACGACGT TGTAAAACGA CGG
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