pDSK-GFPuv
$128.00
In stock
SKU
PL81322
100μg liquid plasmid(Instantly):$163
| name | pDSK-GFPuv | another name | apDSK-GFPuv |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | psbA | Replicon | RSF1010 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC TGGTATGAGT CAGCAACACC TTCTTCACGA GGCAGACCTC AGCGCTATTC TGACCTTGCC ATCACGACTG TGCTGGTCAT TAAACGCGTA TTCAGGCTGA CCCTGCGCGC TGCGCAGGGC TTTATTGATT CCATTTTTAC ACTGATGAAT GTTCCGTTGC GCTGCCCGGA TTACAGCTGC GTTTCCTGTT TGGGGTCGTT TGCGGGAAGG GGCGGAATCC TACGCTAAGG CTTTGGCCAG CGATATTCTC CGGTGAGATT GATGTGTTCC CAGGGGATAG GAGAAGTCGC TTGATATCTA GTATGACGTC TGTCGCACCT GCTTGATCGC GGCCGCGATA GCTAGATCGC GTTGCTCCTC TTCTCCATCC GCGTTCCAAG CTGCGGAAAG GCACCCATAA GCGTACGCCT GGTCGAGCAG GCGACGCGGA TCGACGTCCA GCGCACGAGA GAATGCGTCC GCCATCTGTG CAATGCGTCT AGGATCGAGA CAAAGGTCGT CTCTGTCAGC CGGATCGTAG AACATATTGG CGGCGCCAAA GCCCACTTCA CCGACCAGAC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGGGAT CTAGAGGATC TCAATGAATA TTGGTTGACA CGGGCGTATA AGACATGTTA TACTGTTGAA TAACAAGCTT TCAATTCTCT ATTTTGAGAA GAATTAATGT GCTTGGGAGT CCCTGATGAT TAAATTAAAT AAACCAAGAT TTTACCATGA CTGCAATTTT AGAGAGACGG GGGATCGATC CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGCCAACA CTTGTCACTA CTTTCTCTTA TGGTGTTCAA TGCTTTTCCC GTTATCCGGA TCATATGAAA CGGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAACG CACTATATCT TTCAAAGATG ACGGGAACTA CAAGACGCGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATCGTA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTCGGACAC AAACTCGAGT ACAACTATAA CTCACACAAT GTATACATCA CGGCAGACAA ACAAAAGAAT GGAATCAAAG CTAACTTCAA AATTCGCCAC AACATTGAAG ATGGATCAGT TCAACTAGCA GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCGA CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGCGTGA CCACATGGTC CTTCTTGAGT TTGTAACTGC TGCTGGGATT ACACATGGCA TGGATGAGCT CTACAAATAA GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA AGAAGAGCAT ACATCTGGAA GCAAAGCCAG GAAAGCGGCC TATGGAGCTG TGCGGCAGCG CTCAGTAGGC AATTTTTCAA AATATTGTTA AGCCTTTTCT GAGCATGGTA TTTTTCATGG TATTACCAAT TAGCAGGAAA ATAAGCCATT GAATATAAAA GATAAAAATG TCTTGTTTAC AATAGAGTGG GGGGGGTCAG CCTGCCGCCT TGGGCCGGGT GATGTCGTAC TTGCCCGCCG CGAACTCGGT TACCGTCCAG CCCAGCGCGA CCAGCTCCGG CAACGCCTCG CGCACCCGCT GGCGGCGCTT GCGCATGGTC GAACCACTGG CCTCTGACGG CCAGACATAG CCGCACAAGG TATCTATGGA AGCCTTGCCG GTTTTGCCGG GGTCGATCCA GCCACACAGC CGCTGGTGCA GCAGGCGGGC GGTTTCGCTG TCCAGCGCCC GCACCTCGTC CATGCTGATG CGCACATGCT GGCCGCCACC CATGACGGCC TGCGCGATCA AGGGGTTCAG GGCCACGTAC AGGCGCCCGT CCGCCTCGTC GCTGGCGTAC TCCGACAGCA GCCGAAACCC CTGCCGCTTG CGGCCATTCT GGGCGATGAT GGATACCTTC CAAAGGCGCT CGATGCAGTC CTGTATGTGC TTGAGCGCCC CACCACTATC GACCTCTGCC CCGATTTCCT TTGCCAGCGC CCGATAGCTA CCTTTGACCA CCATGGCATC AGCGGTGACG GCCTCCCACT TGGGTTCCAG GAACAGCCGG AGCTGCCGTC CGCCTTCGGT CTTGGGTTCC GGGCCAAGCA CTAGGCCATT AGGCCCAGCC ATGGCCACCA GCCCTTGCAG GATGCGCAGA TCATCAGCGC CCAGCGGCTC CGGGCCGCTG AACTCGATCC GCTTGCCGTC GCCGTAGTCA TACGTCACGT CCAGCTTGCT GCGCTTGCGC TCGCCCCGCT TGAGGGCACG GAACAGGCCG GGGGCCAGAC AGTGCGCCGG GTCGTGCCGG ACGTGGCTGA GGCTGTGCTT GTTCTTAGGC TTCACCACGG GGCACCCCCT TGCTCTTGCG CTGCCTCTCC AGCACGGCGG GCTTGAGCAC CCCGCCGTCA TGCCGCCTGA ACCACCGATC AGCGAACGGT GCGCCATAGT TGGCCTTGCT CACACCGAAG CGGACGAAGA ACCGGCGCTG GTCGTCGTCC ACACCCCATT CCTCGGCCTC GGCGCTGGTC ATGCTCGACA GGTAGGACTG CCAGCGGATG TTATCGACCA GTACCGAGCT GCCCCGGCTG GCCTGCTGCT GGTCGCCTGC GCCCATCATG GCCGCGCCCT TGCTGGCATG GTGCAGGAAC ACGATAGAGC ACCCGGTATC GGCGGCGATG GCCTCCATGC GACCGATGAC CTGGGCCATG GGGCCGCTGG CGTTTTCTTC CTCGATGTGG AACCGGCGCA GCGTGTCCAG CACCATCAGG CGGCGGCCCT CGGCGGCGCG CTTGAGGCCG TCGAACCACT CCGGGGCCAT GATGTTGGGC AGGCTGCCGA TCAGCGGCTG GATCAGCAGG CCGTCAGCCA CGGCTTGCCG TTCCTCGGCG CTGAGGTGCG CCCCAAGGGC GTGCAGGCGG TGATGAATGG CGGTGGGCGG GTCTTCGGCG GGCAGGTAGA TCACCGGGCC GGTGGGCAGT TCGCCCACCT CCAGCAGATC CGGCCCGCCT GCAATCTGTG CGGCCAGTTG CAGGGCCAGC ATGGATTTAC CGGCACCACC GGGCGACACC AGCGCCCCGA CCGTACCGGC CACCATGTTG GGCAAAACGT AGTCCAGCGG TGGCGGCGCT GCTGCGAACG CCTCCAGAAT ATTGATAGGC TTATGGGTAG CCATTGATTG CCTCCTTTGC AGGCAGTTGG TGGTTAGGCG CTGGCGGGGT CACTACCCCC GCCCTGCGCC GCTCTGAGTT CTTCCAGGCA CTCGCGCAGC GCCTCGTATT CGTCGTCGGT CAGCCAGAAC TTGCGCTGAC GCATCCCTTT GGCCTTCATG CGCTCGGCAT ATCGCGCTTG GCGTACAGCG TCAGGGCTGG CCAGCAGGTC GCCGGTCTGC TTGTCCTTTT GGTCTTTCAT ATCAGTCACC GAGAAACTTG CCGGGGCCGA AAGGCTTGTC TTCGCGGAAC AAGGACAAGG TGCAGCCGTC AAGGTTAAGG CTGGCCATAT CAGCGACTGA AAAGCGGCCA GCCTCGGCCT TGTTTGACGT ATAACCAAAG CCACCGGGCA ACCAATAGCC CTTGTCACTT TTGATCAGGT AGACCGACCC TGAAGCGCTT TTTTCGTATT CCATAAAACC CCCTTCTGTG CGTGAGTACT CATAGTATAA CAGGCGTGAG TACCAACGCA AGCACTACAT GCTGAAATCT GGCCCGCCCC TGTCCATGCC TCGCTGGCGG GGTGCCGGTG CCCGTGCCAG CTCGGCCCGC GCAAGCTGGA CGCTGGGCAG ACCCATGACC TTGCTGACGG TGCGCTCGAT GTAATCCGCT TCGTGGCCGG GCTTGCGCTC TGCCAGCGCT GGGCTGGCCT CGGCCATGGC CTTGCCGATT TCCTCGGCAC TGCGGCCCCG GCTGGCCAGC TTCTGCGCGG CGATAAAGTC GCACTTGCTG AGGTCATCAC CGAAGCGCTT GACCAGCCCG GCCATCTCGC TGCGGTACTC GTCCAGCGCC GTGCGCCGGT GGCGGCTAAG CTGCCGCTCG GGCAGTTCGA GGCTGGCCAG CCTGCGGGCC TTCTCCTGCT GCCGCTGGGC CTGCTCGATC TGCTGGCCAG CCTGCTGCAC CAGCGCCGGG CCAGCGGTGG CGGTCTTGCC CTTGGATTCA CGCAGCAGCA CCCACGGCTG ATAACCGGCG CGGGTGGTGT GCTTGTCCTT GCGGTTGGTG AAGCCCGCCA AGCGGCCATA GTGGCGGCTG TCGGCGCTGG CCGGGTCGGC GTCGTACTCG CTGGCCAGCG TCCGGGCAAT CTGCCCCCGA AGTTCACCGC CTGCGGCGTC GGCCACCTTG ACCCATGCCT GATAGTTCTT CGGGCTGGTT TCCACTACCA GGGCAGGCTC CCGGCCCTCG GCTTTCATGT CATCCAGGTC AAACTCGCTG AGGTCGTCCA CCAGCACCAG ACCATGCCGC TCCTGCTCGG CGGGCCTGAT ATACACGTCA TTGCCCTGGG CATTCATCCG CTTGAGCCAT GGCGTGTTCT GGAGCACTTC GGCGGCTGAC CATTCCCGGT TCATCATCTG GCCGGTGGTG GCGTCCCTGA CGCCGATATC GAAGCGCTCA CAGCCCATGG CCTTGAGCTG TCGGCCTATG GCCTGCAAAG TCCTGTCGTT CTTCATCGGG CCACCAAGCG CAGCCAGATC GAGCCGTCCT CGGTTGTCAG TGGCGTCAGG TCGAGCAAGA GCAACGATGC GATCAGCAGC ACCACCGTAG GCATCATGGA AGCCAGCATC ACGGTTAGCC ATAGCTTCCA GTGCCACCCC CGCGACGCGC TCCGGGCGCT CTGCGCGGCG CTGCTCACCT CGGCGGCTAC CTCCCGCAAC TCTTTGGCCA GCTCCACCCA TGCCGCCCCT GTCTGGCGCT GGGCTTTCAG CCACTCCGCC GCCTGCGCCT CGCTGGCCTG CTGGGTCTGG CTCATGACCT GCCGGGCTTC GTCGGCCAGT GTCGCCATGC TCTGGGCCAG CGGTTCGATC TGCTCCGCTA ACTCGTTGAT GCCTCTGGAT TTCTTCACTC TGTCGATTGC GTTCATGGTC TATTGCCTCC CGGTATTCCT GTAAGTCGAT GATCTGGGCG TTGGCGGTGT CGATGTTCAG GGCCACGTCT GCCCGGTCGG TGCGGATGCC CCGGCCTTCC ATCTCCACCA CGTTCGGCCC CAGGTGAACA CCGGGCAGGC GCTCGATGCC CTGCGCCTCA AGTGTTCTGT GGTCAATGCG GGCGTCGTGG CCAGCCCGCT CTAATGCCCG GTTGGCATGG TCGGCCCATG CCTCGCGGGT CTGCTCAAGC CATGCCTTGG GCTTGAGCGC TTCGGTCTTC TGTGCCCCGC CCTTCTCCGG GGTCTTGCCG TTGTACCGCT TGAACCACTG AGCGGCGGGC CGCTCGATGC CGTCATTGAT CCGCTCGGAG ATCATCAGGT GGCAGTGCGG GTTCTCGCCG CCACCGGCAT GGATGGCCAG CGTATACGGC AGGCGCTCGG CACCGGTCAG GTGCTGGGCG AACTCGGACG CCAGCGCCTT CTGCTGGTCG AGGGTCAGCT CGACCGGCAG GGCAAATTCG ACCTCCTTGA ACAGCCGCCC ATTGGCGCGT TCATACAGGT CGGCAGCATC CCAGTAGTCG GCGGGCCGCT CGACGAACTC CGGCATGTGC CCGGATTCGG CGTGCAAGAC TTCATCCATG TCGCGGGCAT ACTTGCCTTC GCGCTGGATG TAGTCGGCCT TGGCCCTGGC CGATTGGCCG CCCGACCTGC TGCCGGTTTT CGCCGTAAGG TGATAAATCG CCATGCTGCC TCGCTGTTGC TTTTGCTTTT CGGCTCCATG CAATGGCCCT CGGAGAGCGC ACCGCCCGAA GGGTGGCCGT TAGGCCAGTT TCTCGAAGAG AAACCGGTAA GTGCGCCCTC CCCTACAAAG TAGGGTCGGG ATTGCCGCCG CTGTGCCTCC ATGATAGCCT ACGAGACAGC ACATTAACAA TGGGGTGTCA AGATGGTTAA GGGGAGCAAC AAGGCGGCGG ATCGGCTGGC CAAGCTCGAA GAACAACGAG CGCGAATCAA TGCCGAAATT CAGCGGGTGC GGGCAAGGGA ACAGCAGCAA GAGCGCAAGA ACGAAACAAG GCGCAAGGTG CTGGTGGGGG CCATGATTTT GGCCAAGGTG AACAGCAGCG AGTGGCCGGA GGATCGGCTC ATGGCGGCAA TGGATGCGTA CCTTGAACGC GACCACGACC GCGCCTTGTT CGGTCTGCCG CCACGCCAGA AGGATGAGCC GGGCTGAATG ATCGACCGAG ACAGGCCCTG CGGGGCTGCA CACGCGCCCC CACCCTTCGG GTAGGGGGAA AGGCCGCTAA AGCGGCTAAA AGCGCTCCAG CGTATTTCTG CGGGGTTTGG TGTGGGGTTT AGCGGGCTTT GCCCGCCTTT CCCCCTGCCG CGCAGCGGTG GGGCGGTGTG TAGCCTAGCG CAGCGAATAG ACCAGCTATC CGGCCTCTGG CCGGGCATAT TGGGCAAGGG CAGCAGCGCC CCACAAGGGC GCTGATAACC GCGCCTAGTG GATTATTCTT AGATAATCAT GGATGGATTT TTCCAACACC CCGCCAGCCC CCGCCCCTGC TGGGTTTGCA GGTTTGGGGG CGTGACAGTT ATTGCAGGGG TTCGTGACAG TTATTGCAGG GGGGCGTGAC AGTTATTGCA GGGGTTCGTG ACAGTTAGTA CGGGAGTGAC GGGCACTGGC TGGCAATGTC TAGCAACGGC AGGCATTTCG GCTGAGGGTA AAAGAACTTT CCGCTAAGCG ATAGACTGTA TGTAAACACA GTATTGCAAG GACGCGGAAC ATGCCTCATG TGGCGGCCAG GACGGCCAGC CGGGATCGGG ATACTGGTCG TTACCAGAGC CACCGACCCG AGCAAACCCT TCTCTATCAG ATCGTTGACG AGTATTACCC GGCATTCGCT GCGCTTATGG CAGAGCAGGG AAAGGAATTG CCGGGCTATG TGCAACGGGA ATTTGAAGAA TTTCTCCAAT GCGGGCGGCT GGAGCATGGC TTTCTACGGG TTCGCTGCGA GTCTTGCCAC GCCGAGCACC TGGTCGCTTT CAGCTGTAAT CCGGGCAGCG CAACGGAACA TTCATCAGTG TAAAAATGGA ATCAATAAAG CCCTGCGCAG CGCGCAGGGT CAGCCTGAAT ACGCGTTTAA TGACCAGCAC AGTCGTGATG GCAAGGTCAG AATAGCGCTG AGGTCTGCCT CGTGAAGAAG GTGTTGCTGA CTCATACCAG GCCTGAATCG CCCCATCATC CAGCCAGAAA GTGAGGGAGC CACGGTTGAT GAGAGCTTTG TTGTAGGTGG ACCAGTTGGT GATTTTGAAC TTTTGCTTTG CCACGGAACG GTCTGCGTTG TCGGGAAGAT GCGTGATCTG ATCCTTCAAC TCAGCAAAAG TTCGATTTAT TCAACAAAGC CGCCGTCCCG TCAAGTCAGC GTAATGCTCT GCCAGTGTTA CAACCAATTA ACCAATTCTG ATTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGCTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTCGAGCA AGACGTTTCC CGTTGAATAT GGCTCATAGC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA
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