pMP2444
$128.00
In stock
SKU
PL81147
100μg liquid plasmid(Instantly):$149
| name | pMP2444 | another name | apMP2444 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Gen | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCTTCGGGA GCGCCTGAAG CCCGTTCTGG ACGCCCTGGG GCCGTTGAAT CGGGATATGC AGGCCAAGGC CGCCGCGATC ATCAAGGCCG TGGGCGAAAA GCTGCTGACG GAACAGCGGG AAGTCCAGCG CCAGAAACAG GCCCAGCGCC AGCAGGAACG CGGGCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGCGGCG TTGTGACAAT TTACCGAACA ACTCCGCGGC CGGGAAGCCG ATCTCGGCTT GAACGAATTG TTAGGTGGCG GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT TGTATAGAGA GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC ACCAAGCGCG TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT CCGGTGCTCG CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA CCTGGGCAGA ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG CGCGATGAAT GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG GGAGTAGGTG GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA TTTGACTTGG TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATCGTTGC TGCTCCATAA CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG TACAAAAAAA CAGTCATAAC AAGCCATGAA AACCGCCACT GCGCCGTTAC CACCGCTGCG TTCGGTCAAG GTTCTGGACC AGTTGCGTGA GCGCATACGC TACTTGCATT ACAGTTTACG AACCGAACAG GCTTATGTCA ACTGGGTTCG TGCCTTCATC CGTTTCCACG GTGTGCGTCC ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTTTTTA TGCATGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG GGGGATCCAA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAGT CTAGATTAGT GGAGCTCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAC TGCGATGAGT GGCAGGGCGG GGCGTAATTT TTTTAAGGCA GTTATTGGTG CCCTTAAACG CCTGGTGCTA CGCCTGAATA AGTGATAATA AGCGGATGAA TGGCAGAAAT TCGAAAGCAA ATTCGACCCG GTCGTCGGTT CAGGGCAGGG TCGTTAAATA GCCGCTTATG TCTATTGCTG GTTTACCGGT TTATTGACTA CCGGAAGCAG TGTGACCGTG TGCTTCTCAA ATGCCTGAGG CCAGTTTGCT CAGGCTCTCC CCGTGGAGGT AATAATTGAC GATATGATCA TTTATTCTGC CTCCCAGAGC CTGATAAAAA CGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGAGGC GGCTACAGCC GATAGTCTGG AACAGCGCAC TTACGGGTTG CTGCGCAACC CAAGTGCTAC CGGCGCGGCA GCGTGACCCG TGTCGGCGGC TCCAACGGCT CGCCATCGTC CAGAAAACAC GGCTCATCGG GCATCGGCAG GCGCTGCTGC CCGCGCCGTT CCCATTCCTC CGTTTCGGTC AAGGCTGGCA GGTCTGGTTC CATGCCCGGA ATGCCGGGCT GGCTGGGCGG CTCCTCGCCG GGGCCGGTCG GTAGTTGCTG CTCGCCCGGA TACAGGGTCG GGATGCGGCG CAGGTCGCCA TGCCCCAACA GCGATTCGTC CTGGTCGTCG TGATCAACCA CCACGGCGGC ACTGAACACC GACAGGCGCA ACTGGTCGCG GGGCTGGCCC CACGCCACGC GGTCATTGAC CACGTAGGCC GACACGGTGC CGGGGCCGTT GAGCTTCACG ACGGAGATCC AGCGCTCGGC CACCAAGTCC TTGACTGCGT ATTGGACCGT CCGCAAAGAA CGTCCGATGA GCTTGGAAAG TGTCTTCTGG CTGACCACCA CGGCGTTCTG GTGGCCCATC TGCGCCACGA GGTGATGCAG CAGCATTGCC GCCGTGGGTT TCCTCGCAAT AAGCCCGGCC CACGCCTCAT GCGCTTTGCG TTCCGTTTGC ACCCAGTGAC CGGGCTTGTT CTTGGCTTGA ATGCCGATTT CTCTGGACTG CGTGGCCATG CTTATCTCCA TGCGGTAGGG TGCCGCACGG TTGCGGCACC ATGCGCAATC AGCTGCAACT TTTCGGCAGC GCGACAACAA TTATGCGTTG CGTAAAAGTG GCAGTCAATT ACAGATTTTC TTTAACCTAC GCAATGAGCT ATTGCGGGGG GTGCCGCAAT GAGCTGTTGC GTACCCCCCT TTTTTAAGTT GTTGATTTTT AAGTCTTTCG CATTTCGCCC TATATCTAGT TCTTTGGTGC CCAAAGAAGG GCACCCCTGC GGGGTTCCCC CACGCCTTCG GCGCGGCTCC CCCTCCGGCA AAAAGTGGCC CCTCCGGGGC TTGTTGATCG ACTGCGCGGC CTTCGGCCTT GCCCAAGGTG GCGCTGCCCC CTTGGAACCC CCGCACTCGC CGCCGTGAGG CTCGGGGGGC AGGCGGGCGG GCTTCGCCTT CGACTGCCCC CACTCGCATA GGCTTGGGTC GTTCCAGGCG CGTCAAGGCC AAGCCGCTGC GCGGTCGCTG CGCGAGCCTT GACCCGCCTT CCACTTGGTG TCCAACCGGC AAGCGAAGCG CGCAGGCCGC AGGCCGGAGG CTTTTCCCCA GAGAAAATTA AAAAAATTGA TGGGGCAAGG CCGCAGGCCG CGCAGTTGGA GCCGGTGGGT ATGTGGTCGA AGGCTGGGTA GCCGGTGGGC AATCCCTGTG GTCAAGCTCG TGGGCAGGCG CAGCCTGTCC ATCAGCTTGT CCAGCAGGGT TGTCCACGGG CCGAGCGAAG CGAGCCAGCC GGTGGCCGCT CGCGGCCATC GTCCACATAT CCACGGGCTG GCAAGGGAGC GCAGCGACCG CGCAGGGCGA AGCCCGGAGA GCAAGCCCGT AGGGCGCCGC AGCCGCCGTA GGCGGTCACG ACTTTGCGAA GCAAAGTCTA GTGAGTATAC TCAAGCATTG AGTGGCCCGC CGGAGGCACC GCCTTGCGCT GCCCCCGTCG AGCCGGTTGG ACACCAAAAG GGAGGGGCAG GCATGGCGGC ATACGCGATC ATGCGATGCA AGAAGCTGGC GAAAATGGGC AACGTGGCGG CCAGTCTCAA GCACGCCTAC CGCGAGCGCG AGACGCCCAA CGCTGACGCC AGCAGGACGC CAGAGAACGA GCACTGGGCG GCCAGCAGCA CCGATGAAGC GATGGGCCGA CTGCGCGAGT TGCTGCCAGA GAAGCGGCGC AAGGACGCTG TGTTGGCGGT CGAGTACGTC ATGACGGCCA GCCCGGAATG GTGGAAGTCG GCCAGCCAAG AACAGCAGGC GGCGTTCTTC GAGAAGGCGC ACAAGTGGCT GGCGGACAAG TACGGGGCGG ATCGCATCGT GACGGCCAGC ATCCACCGTG ACGAAACCAG CCCGCACATG ACCGCGTTCG TGGTGCCGCT GACGCAGGAC GGCAGGCTGT CGGCCAAGGA GTTCATCGGC AACAAAGCGC AGATGACCCG CGACCAGACC ACGTTTGCGG CCGCTGTGGC CGATCTAGGG CTGCAACGGG GCATCGAGGG CAGCAAGGCA CGTCACACGC GCATTCAGGC GTTCTACGAG GCCCTGGAGC GGCCACCAGT GGGCCACGTC ACCATCAGCC CGCAAGCGGT CGAGCCACGC GCCTATGCAC CGCAGGGATT GGCCGAAAAG CTGGGAATCT CAAAGCGCGT TGAGACGCCG GAAGCCGTGG CCGACCGGCT GACAAAAGCG GTTCGGCAGG GGTATGAGCC TGCCCTACAG GCCGCCGCAG GAGCGCGTGA GATGCGCAAG AAGGCCGATC AAGCCCAAGA GACGGCCCGA G
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