pHANNIBAL
$128.00
In stock
SKU
PL81135
100μg liquid plasmid(Instantly):$149
| name | pHANNIBAL | another name | apHANNIBAL |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | 35S | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGACCAAGTC AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAAAT TGTAAACGTT AATATTTTGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT TGGCCAAGTC GGCCTCTAAT ACGACTCACT ATAGGGAGCT CGTCGAGCGG CCGCTCGACG AATTAATTCC AATCCCACAA AAATCTGAGC TTAACAGCAC AGTTGCTCCT CTCAGAGCAG AATCGGGTAT TCAACACCCT CATATCAACT ACTACGTTGT GTATAACGGT CCACATGCCG GTATATACGA TGACTGGGGT TGTACAAAGG CGGCAACAAA CGGCGTTCCC GGAGTTGCAC ACAAGAAATT TGCCACTATT ACAGAGGCAA GAGCAGCAGC TGACGCGTAC ACAACAAGTC AGCAAACAGA CAGGTTGAAC TTCATCCCCA AAGGAGAAGC TCAACTCAAG CCCAAGAGCT TTGCTAAGGC CCTAACAAGC CCACCAAAGC AAAAAGCCCA CTGGCTCACG CTAGGAACCA AAAGGCCCAG CAGTGATCCA GCCCCAAAAG AGATCTCCTT TGCCCCGGAG ATTACAATGG ACGATTTCCT CTATCTTTAC GATCTAGGAA GGAAGTTCGA AGGTGAAGGT GACGACACTA TGTTCACCAC TGATAATGAG AAGGTTAGCC TCTTCAATTT CAGAAAGAAT GCTGACCCAC AGATGGTTAG AGAGGCCTAC GCAGCAGGTC TCATCAAGAC GATCTACCCG AGTAACAATC TCCAGGAGAT CAAATACCTT CCCAAGAAGG TTAAAGATGC AGTCAAAAGA TTCAGGACTA ATTGCATCAA GAACACAGAG AAAGACATAT TTCTCAAGAT CAGAAGTACT ATTCCAGTAT GGACGATTCA AGGCTTGCTT CATAAACCAA GGCAAGTAAT AGAGATTGGA GTCTCTAAAA AGGTAGTTCC TACTGAATCT AAGGCCATGC ATGGAGTCTA AGATTCAAAT CGAGGATCTA ACAGAACTCG CCGTGAAGAC TGGCGAACAG TTCATACAGA GTCTTTTACG ACTCAATGAC AAGAAGAAAA TCTTCGTCAA CATGGTGGAG CACGACACTC TGGTCTACTC CAAAAATGTC AAAGATACAG TCTCAGAAGA CCAAAGGGCT ATTGAGACTT TTCAACAAAG GATAATTTCG GGAAACCTCC TCGGATTCCA TTGCCCAGCT ATCTGTCACT TCATCGAAAG GACAGTAGAA AAGGAAGGTG GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCTAT CATTCAAGAT CTCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT GTGACATCTC CACTGACGTA AGGGATGACG CACAATCCCA CTATCCTTCG CAAGACCCTT CCTCTATATA AGGAAGTTCA TTTCATTTGG AGAGGACACG CTCGAGGAAT TCGGTACCCC AATTGGTAAG GAAATAATTA TTTTCTTTTT TCCTTTTAGT ATAAAATAGT TAAGTGATGT TAATTAGTAT GATTATAATA ATATAGTTGT TATAATTGTG AAAAAATAAT TTATAAATAT ATTGTTTACA TAAACAACAT AGTAATGTAA AAAAATATGA CAAGTGATGT GTAAGACGAA GAAGATAAAA GTTGAGAGTA AGTATATTAT TTTTAATGAA TTTGATCGAA CATGTAAGAT GATATACTAG CATTAATATT TGTTTTAATC ATAATAGTAA TTCTAGCTGG TTTGATGAAT TAAATATCAA TGATAAAATA CTATAGTAAA AATAAGAATA AATAAATTAA AATAATATTT TTTTATGATT AATAGTTTAT TATATAATTA AATATCTATA CCATTACTAA ATATTTTAGT TTAAAAGTTA ATAAATATTT TGTTAGAAAT TCCAATCTGC TTGTAATTTA TCAATAAACA AAATATTAAA TAACAAGCTA AAGTAACAAA TAATATCAAA CTAATAGAAA CAGTAATCTA ATGTAACAAA ACATAATCTA ATGCTAATAT AACAAAGCGC AAGATCTATC AATTTTATAT AGTATTATTT TTCAATCAAC ATTCTTATTA ATTTCTAAAT AATACTTGTA GTTTTATTAA CTTCTAAATG GATTGACTAT TAATTAAATG AATTAGTCGA ACATGAATAA ACAAGGTAAC ATGATAGATC ATGTCATTGT GTTATCATTG ATCTTACATT TGGATTGATT ACAGTTGGGA AGCTGGGTTC GAAATCGATA AGCTTGGATC CTCTAGAGTC CTGCTTTAAT GAGATATGCG AGACGCCTAT GATCGCATGA TATTTGCTTT CAATTCTGTT GTGCACGTTG TAAAAAACCT GAGCATGTGT AGCTCAGATC CTTACCGCCG GTTTCGGTTC ATTCTAATGA ATATATCACC CGTTACTATC GTATTTTTAT GAATAATATT CTCCGTTCAA TTTACTGATT GTACCCTACT ACTTATATGT ACAATATTAA AATGAAAACA ATATATTGTG CTGAATAGGT TTATAGCGAC ATCTATGATA GAGCGCCACA ATAACAAACA ATTGCGTTTT ATTATTACAA ATCCAATTTT AAAAAAAGCG GCAGAACCGG TCAAACCTAA AAGACTGATT ACATAAATCT TATTCAAATT TCAAAAGGCC CCAGGGGCTA GTATCTACGA CACACCGAGC GGCGAACTAA TAACGTTCAC TGAAGGGAAC TCCGGTTCCC CGCCGGCGCG CATGGGTGAG ATTCCTTGAA GTTGAGTATT GGCCGTCCGC TCTACCGAAA GTTACGGGCA CCATTCAACC CGGTCCAGCA CGGCGGCCGG GTAACCGACT TGCTGCCCCG AGAATTATGC AGCATTTTTT TGGTGTATGT GGGCCCCAAA TGAAGTGCAG GTCAAACCTT GACAGTGACG ACAAATCGTT GGGCGGGTCC AGGGCGAATT TTGCGACAAC ATGTCGAGGC TCAGCAGGAC CTGCAGGCAT GCAAGCTAGC TTACTAGTGA TGCATATTCT ATAGTGTCAC CTAAATCTGC GGCCGC
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