pCDNA3.1-Ano1-m-T2A-EYFP-H148Q-I152L
$113.00
In stock
SKU
PL81134
NM_178642.5
100μg liquid plasmid(Instantly):$134
| name | pCDNA3.1-Ano1-m-T2A-EYFP-H148Q-I152L | another name | apCDNA3.1-Ano1-m-T2A-EYFP-H148Q-I152L |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Yellow |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCGCCACCA TGCAGGACGC GCAGGACAGC GACATCGGCC TGGAGGGCTT GACCCCAGAG GGAACCTCTG CGGACCGAGA GTGCCAGCGG GGACCGGAAA CAATCGCGCA CGAGGCACAG GACGCAGGAA CCCCCAACTC AGACGCCACC GGGGTTGTGG ATGGGGAGCG CGAGGCCACC ATGAGGGTCC CCGAGAAGTA CTCGACGCTC CCGGCGGAGG ACCGCAGCGT CCACATCGTG AACATCTGCG CCATCGAGGA CCTGGGCTAC CTGCCGTCCG AGGGCACGTT GCTGAACTCT CTGTCCGTGG ACCCCGACGC CGAATGCAAG TATGGACTAT ACTTCAGGGA TGGCAAACGG AAGGTGGACT ACATCTTGGT ATACCATCAC AAGAGAGCCT CAGGGAGCAG GACTCTGGCC AGGAGGGGAC TACAAAATGA CATGGTCCTG GGGACCCGCA GCGTCAGGCA GGACCAGCCC CTTCCCGGGA AGGGGAGCCC TGTGGATGCA GGCTCACCGG AAGTCCCCAT GGATTACCAT GAAGATGACA AACGCTTCAG ACGGGAGGAA TATGAGGGCA ACCTGTTGGA GGCAGGCCTG GAGTTGGAGA ATGACGAGGA TACCAAAATC CATGGTGTCG GGTTTGTGAA GATCCATGCG CCCTGGCATG TGCTCTGTAG GGAAGCTGAG TTTTTGAAAC TAAAGATGCC CACAAAGAAG GTGTACCACA TCAGTGAGAC GCGAGGCCTC CTGAAAACCA TCAACTCGGT TCTGCAGAAG ATCACAGACC CCATCCAGCC CAAGGTGGCT GAGCACAGGC CACAGACCAC AAAGAGGCTC TCCTATCCCT TCTCCCGGGA GAAGCAACAC CTATTCGACC TGACTGACAG GGACTCTTTT TTCGACAGCA AAACCCGGAG CACAATAGTC TATGAAATCC TGAAGAGAAC AACGTGCACC AAGGCCAAGT ACAGCATGGG TATCACCAGC CTCCTGGCCA ATGGCGTATA CTCAGCTGCA TACCCTCTGC ACGATGGGGA CTATGAGGGT GACAACGTTG AGTTCAACGA CAGGAAACTC CTGTATGAGG AATGGGCAAG TTACGGAGTC TTCTACAAAT ACCAGCCCAT TGACCTGGTC AGGAAATACT TTGGTGAGAA GGTTGGCCTG TACTTTGCCT GGCTTGGAGC CTACACCCAG ATGCTCATCC CTGCCTCGAT CGTGGGTGTC ATTGTCTTTC TCTATGGATG TGCCACTGTG GACGAAAACA TCCCCAGTAT GGAGATGTGT GACCAGAGAT ACAACATCAC CATGTGTCCT CTGTGTGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGTGCCAC AGCCCGTGCC AGTCACCTTT TTGATAACCC TGCCACCGTC TTCTTCTCTG TGTTTATGGC CCTCTGGGCT GCCACTTTCA TGGAGCACTG GAAACGGAAG CAGATGAGGC TCAACTACCG ATGGGACCTC ACAGGCTTCG AGGAGGAGGA GGAAGCTGTC AAGGATCATC CCAGAGCAGA GTATGAAGCC AGAGTCTTAG AGAAGTCACT GAGAAAAGAA TCCAGAAACA AAGAGACCGA CAAGGTGAAG CTGACCTGGA GGGACCGATT CCCAGCCTAT TTCACCAATC TTGTCTCCAT CATCTTCATG ATCGCAGTGA CATTTGCAAT CGTCCTCGGA GTTATCATCT ATAGAATCTC CACAGCTGCA GCCTTGGCCA TGAACTCCTC CCCGTCTGTG CGGTCCAACA TCCGGGTTAC AGTCACGGCC ACCGCTGTTA TCATCAACCT CGTGGTCATC ATTCTGCTGG ATGAAGTTTA CGGCTGCATT GCCAGGTGGC TCACCAAGAT TGAGGTCCCA AAGACAGAGA AGAGCTTTGA GGAGAGGCTA ACCTTCAAGG CCTTCCTGCT CAAGTTTGTG AACTCTTACA CTCCCATCTT CTATGTCGCC TTCTTCAAAG GCCGGTTTGT TGGTCGGCCC GGTGACTACG TGTACATCTT CCGCTCTTTC CGGATGGAGG AGTGTGCCCC GGGCGGCTGC CTCATGGAGC TCTGTATCCA GCTGAGCATC ATTATGCTGG GCAAGCAGCT AATCCAGAAC AATCTCTTCG AGATTGGCAT CCCGAAGATG AAAAAGTTCA TCCGCTACCT GAAGCTGCGC AGACAGAGCC CCTCAGACCG TGAAGAGTAC GTGAAGCGGA AGCAGCGCTA TGAGGTGGAC TTCAACCTCG AACCTTTCGC CGGCCTCACG CCCGAGTACA TGGAAATGAT CATTCAGTTC GGCTTTGTCA CCCTGTTTGT TGCGTCCTTC CCTCTGGCTC CACTCTTCGC CCTGCTAAAC AACATCATTG AGATCCGCCT GGATGCCAAA AAGTTTGTCA CCGAGCTACG GAGGCCAGTA GCCATCAGAG CCAAAGACAT CGGCATCTGG TATAACATCC TCAGAGGTGT TGGGAAGCTG GCTGTCATCA TTAATGCCTT TGTGATCTCC TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTCA GAATGGGACC ATGCACGGCT TCGTCAATCA CACGCTCTCT TCCTTCAATG TCAGCGACTT CCAGAATGGC ACAGCACCCA ATGACCCACT GGACCTGGGC TATGAGGTTC AGATCTGCAG GTATAAAGAT TACCGGGAAC CCCCATGGTC AGAACACAAG TATGACATCT CCAAAGACTT CTGGGCTGTC CTGGCCGCCC GACTGGCATT TGTCATTGTC TTCCAGAACC TGGTGATGTT CATGAGTGAC TTTGTGGACT GGGTGATCCC TGATATCCCC AAAGACATCA GCCAGCAGAT CCACAAAGAG AAGGTTCTCA TGGTGGAGCT GTTTATGCGT GAGGAGCAGG GCAAGCAGCA GCTACTGGAC ACATGGATGG AGAAGGAGAA GCCAAGGGAT GTGCCTTGTA ACAACCACAG CCCCACAACC CACCCAGAGG CAGGCGACGG CAGCCCAGTC CCCAGCTACG AGTACCATGG GGACGCGCTG GGTGGTGGAG GATCTGAGGG CAGAGGAAGT CTTCTAACAT GCGGTGACGT GGAGGAGAAT CCCGGCCCTC GGGTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCAGAACGTC TATCTGATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGCGACT CTAGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT
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