pActin-EGFP-beta-Actin (Homo)
$113.00
In stock
SKU
PL81036
ACTB;BRWS1;PS1TP5BP1
100μg liquid plasmid(Instantly):$134
| name | pActin-EGFP-beta-Actin (Homo) | another name | apActin-EGFP-beta-Actin (Homo) |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | betaActin | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTTGG CCAGCTGAAT GGAGGCCCAG CGGCAACACA GGTCCTGCCT GGGGATCAGG TCTGCTCTGC ACCCCACCTT GCTGCCTGGA GCCGCCCACC TGACAACCTC TCATCCCTGC TCTGTAGATC CGGTCCCATC CCCACTGCCC ACCCCACCCC CCCAGCACTC CACCCAGTTC AACGTTCCAC GAACCCCCAG AACCAGCCCT CATCAACAGG CAGCAAGAAG GGCCCCCCGC CCATCGCCCC ACAACGCCAG CCGGGTGAAC TGTAGCGTTG GCAGGTCCTG AGGCAGCTGG CAAGACGCCT GCAGCTGAAA GATACAAGGC CAGGGACAGG ACAGTCCCAT CCCCAGGAGG CAGGGAGTAT ACAGGCTGGG GAAGTTTGCC CTTGCGTGGG GTGGTGATGG AGGAGGCTCA GCAAGTCTTC TGGACTGTGA ACCTGTGTCT GCCACTGTGT GCTGGGTGGT GGTCATCTTT CCCACCAGGC TGTGGCCTCT GCAACCTTCA AGGGAGGAGC AGGTCCCATT GGCTGAGCAC AGCCTTGTAC CGTGAACTGG AACAAGCAGC CTCCTTCCTG GCCACAGGTT CCATGTCCTT ATATGGACTC ATCTTTGCCT ATTGCGACAC ACACTCAATG AACACCTACT ACGCGCTGCA AAGAGCCCCG CAGGCCTGAG GTGCCCCCAC CTCACCACTC TTCCTATTTT TGTGTAAAAA TCCAGCTTCT TGTCACCACC TCCAAGGAGG GGGAGGAGGA GGAAGGCAGG TTCCTCTAGG CTGAGCCGAA TGCCCCTCTG TGGTCCCACG CCACTGATCG CTGCATGCCC ACCACCTGGG TACACACAGT CTGTGATTCC CGGAGCAGAA CGGACCCTGC CCACCCGGTC TTGTGTGCTA CTCAGTGGAC AGACCCAAGG CAAGAAAGGG TGACAAGGAC AGGGTCTTCC CAGGCTGGCT TTGAGTTCCT AGCACCGCCC CGCCCCCAAT CCTCTGTGGC ACATGGAGTC TTGGTCCCCA GAGTCCCCCA GCGGCCTCCA GATGGTCTGG GAGGGCAGTT CAGCTGTGGC TGCGCATAGC AGACATACAA CGGACGGTGG GCCCAGACCC AGGCTGTGTA GACCCAGCCC CCCCGCCCCG CAGTGCCTAG GTCACCCACT AACGCCCCAG GCCTGGTCTT GGCTGGGCGT GACTGTTACC CTCAAAAGCA GGCAGCTCCA GGGTAAAAGG TGCCCTGCCC TGTAGAGCCC ACCTTCCTTC CCAGGGCTGC GGCTGGGTAG GTTTGTAGCC TTCATCACGG GCCACCTCCA GCCACTGGAC CGCTGGCCCC TGCCCTGTCC TGGGGAGTGT GGTCCTGCGA CTTCTAAGTG GCCGCAAGCC ACCTGACTCC CCCAACACCA CACTCTACCT CTCAAGCCCA GGTCTCTCCC TAGTGACCCA CCCAGCACAT TTAGCTAGCT GAGCCCCACA GCCAGAGGTC CTCAGGCCCT GCTTTCAGGG CAGTTGCTCT GAAGTCGGCA AGGGGGAGTG ACTGCCTGGC CACTCCATGC CCTCCAAGAG CTCCTTCTGC AGGAGCGTAC AGAACCCAGG GCCCTGGCAC CCGTGCAGAC CCTGGCCCAC CCCACCTGGG CGCTCAGTGC CCAAGAGATG TCCACACCTA GGATGTCCCG CGGTGGGTGG GGGGCCCGAG AGACGGGCAG GCCGGGGGCA GGCCTGGCCA TGCGGGGCCG AACCGGGCAC TGCCCAGCGT GGGGCGCGGG GGCCACGGCG CGCGCCCCCA GCCCCCGGGC CCAGCACCCC AAGGCGGCCA ACGCCAAAAC TCTCCCTCCT CCTCTTCCTC AATCTCGCTC TCGCTCTTTT TTTTTTTCGC AAAAGGAGGG GAGAGGGGGT AAAAAAATGC TGCACTGTGC GGCGAAGCCG GTGAGTGAGC GGCGCGGGGC CAATCAGCGT GCGCCGTTCC GAAAGTTGCC TTTTATGGCT CGAGCGGCCG CGGCGGCGCC CTATAAAACC CAGCGGCGCG ACGCGCCACC ACCGCCGAGA CCGCGTCCGC CCCGCGAGCA CAGAGCCTCG CCTTTGCCGA TCCGCCGCCC GTCCACACCC GCCGCCAGGT AAGCCCGGCC AGCCGACCGG GGCATGCGGC CGCGGCCCCT TCGCCCGTGC AGAGCCGCCG TCTGGGCCGC AGCGGGGGGC GCATGGGGGG GGAACCGGAC CGCCGTGGGG GGCGCGGGAG AAGCCCCTGG GCCTCCGGAG ATGGGGGACA CCCCACGCCA GTTCGGAGGC GCGAGGCCGC GCTCGGGAGG CGCGCTCCGG GGGTGCCGCT CTCGGGGCGG GGGCAACCGG CGGGGTCTTT GTCTGAGCCG GGCTCTTGCC AATGGGGATC GCAGGGTGGG CGCGGCGTAG CCCCCGCCAG GCCCGGTGGG GGCTGGGGCG CCATTGCGCG TGCGCGCTGG TCCTTTGGGC GCTAACTGCG TGCGCGCTGG GAATTGGCGC TAATTGCGCG TGCGCGCTGG GACTCAAGGC GCTAATTGCG CGTGCGTTCT GGGGCCCGGG GTGCCGCGGC CTGGGCTGGG GCGAAGGCGG GCTCGGCCGG AAGGGGTGGG GTCGCCGCGG CTCCCGGGCG CTTGCGCGCA CTTCCTGCCC GAGCCGCTGG CCGCCCGAGG GTGTGGCCGC TGCGTGCGCG CGCGCCGACC CGGCGCTGTT TGAACCGGGC GGAGGCGGGG CTGGCGCCCG GTTGGGAGGG GGTTGGGGCC TGGCTTCCTG CCGCGCGCCG CGGGGACGCC TCCGACCAGT GTTTGCCTTT TATGGTAATA ACGCGGCCGG CCCGGCTTCC TTTGTCCCCA ATCTGGGCGC GCGCCGGCGC CCCCTGGCGG CCTAAGGACT CGGCGCGCCG GAAGTGGCCA GGGCGGGGGC GACCTCGGCT CACAGCGCGC CCGGCTATTC TCGCAGCTCA AGCTTCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGA TCCACTAGTG GCATGGATGA TGATATCGCC GCGCTCGTCG TCGACAACGG CTCCGGCATG TGCAAGGCCG GCTTCGCGGG CGACGATGCC CCCCGGGCCG TCTTCCCCTC CATCGTGGGG CGCCCCAGGC ACCAGGGCGT GATGGTGGGC ATGGGTCAGA AGGATTCCTA TGTGGGCGAC GAGGCCCAGA GCAAGAGAGG CATCCTCACC CTGAAGTACC CCATCGAGCA CGGCATCGTC ACCAACTGGG ACGACATGGA GAAAATCTGG CACCACACCT TCTACAATGA GCTGCGTGTG GCTCCCGAGG AGCACCCCGT GCTGCTGACC GAGGCCCCCC TGAACCCCAA GGCCAACCGC GAGAAGATGA CCCAGATCAT GTTTGAGACC TTCAACACCC CAGCCATGTA CGTTGCTATC CAGGCTGTGC TATCCCTGTA CGCCTCTGGC CGTACCACTG GCATCGTGAT GGACTCCGGT GACGGGGTCA CCCACACTGT GCCCATCTAC GAGGGGTATG CCCTCCCCCA TGCCATCCTG CGTCTGGACC TGGCTGGCCG GGACCTGACT GACTACCTCA TGAAGATCCT CACCGAGCGC GGCTACAGCT TCACCACCAC GGCCGAGCGG GAAATCGTGC GTGACATTAA GGAGAAGCTG TGCTACGTCG CCCTGGACTT CGAGCAAGAG ATGGCCACGG CTGCTTCCAG CTCCTCCCTG GAGAAGAGCT ACGAGCTGCC TGACGGCCAG GTCATCACCA TTGGCAATGA GCGGTTCCGC TGCCCTGAGG CACTCTTCCA GCCTTCCTTC CTGGGCATGG AGTCCTGTGG CATCCACGAA ACTACCTTCA ACTCCATCAT GAAGTGTGAC GTGGACATCC GCAAAGACCT GTACGCCAAC ACAGTGCTGT CTGGCGGCAC CACCATGTAC CCTGGCATTG CCGACAGGAT GCAGAAGGAG ATCACTGCCC TGGCACCCAG CACAATGAAG ATCAAGATCA TTGCTCCTCC TGAGCGCAAG TACTCCGTGT GGATCGGCGG CTCCATCCTG GCCTCGCTGT CCACCTTCCA GCAGATGTGG ATCAGCAAGC AGGAGTATGA CGAGTCCGGC CCCTCCATTG TCCACCGCAA ATGCTTCTAG GAATTCTGCA GATATCCATC ACACTGGCGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
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