pRI201-ON
$128.00
In stock
SKU
PL81030
单子叶发根
100μg liquid plasmid(Instantly):$149
| name | pRI201-ON | another name | apRI201-ON |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35s | Replicon | RI |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCGA TCCTACAAGG TAGAATCCGC CTGAGTCGCA AGGGTGACTT CGCCTATATT GGACGACGGC GCGCAGAGGG CGACCTCTTT TTGGGTTACG ATTGTAGGAT TATCACTAAA ACAATACATG AACATATTCA AATGGCAATC TCTCTAAGGC ATTGGAAATA AATACAAATA ACAGTTGGGT GGAGTTTTTC GACCTGAGGG CGTTAACCTT CTGTTAACCT AAAAGCTCTT GCCCAAACAG CAGAATCGGC GCTAATTGCC AGCGGCGGAA CTTTTCCAGT TTCGCGAAAA ATATCGCCAC TGGCAAGGAA TGGGTTTGAG ATGGCGAAGT CTGTCCTAAA AGCAGCGCCT GTAGTTGTAG GGTTGACGGC CTTGATGGAG CGTCATGCCG ATGCCCTCTC GAGCCAACTT CAAGCACATC ATCTTAAGGT TTTCCCGCCG CATTCCGAGA AGGGTATTCG AACATTCGGG CCATCGGAGG CGTCCAAGCT GCTCGGCGTT GGCGAGTCAT ATTTACGGCA GACCGCGTCT GAGATGCCAG AGTTGAATGT TAGCATGAGC CCAGGTGGCA GGCGAATGTT CTCAATTGAA GATATCCATG TGATTCGGAA GTATATGGAT CAGGTCGGCC GCGGGAACCG GCGCTACCTG CCACATCGTC GAGGCGGCGA GCAGCTTCAG GTTATCTCTG TGATGAATTT CAAAGGTGGG TCGGGTAAGA CCACCACCGC CGCGCATCTG GCGCAGTACC TCGCTATGCG CGGATATCGA GTCTTGGCCA TTGATCTCGA TCCTCAAGCG AGCCTTTCTG CACTCTTTGG GAGCCAACCG GAGACGGACG TTGGCCCGAA CGAAACGCTC TACGGCGCTA TAAGGTATGA TGATGAGCAG GTGGCAATCG AACGAGTCGT CCGAGGGACT TACATTCCCG ACCTCCACCT GATTCCTGGT AACCTTGAGC TGATGGAGTT TGAACACGAT ACGCCACGCG CGCTGATGAA CCGCAAAGAG GGCGACACGC TCTTTTATGG TCGCATCAGC CAAGTAATTG AAGATATCGC GGATAACTAT GACGTCGTGG TCATCGACTG CCCTCCCCAG CTTGGGTATC TCACGCTATC CGCATTGACT GCGGCGACGT CCATTCTTGT CACGGTCCAT CCGCAGATGC TGGATGTGAT GTCGATGAAC CAGTTTCTGG CAATGACATC GAACCTTTTG CGTGAAATCG AGAATGCTGG CGCCAAGTTC AAGTTTAATT GGATGCGCTA TCTGATAACC CGTTTCGAAC CGAGCGACGG ACCACAGAAC CAAATGGTAG GTTATCTGCG GTCGATTTTT GGCGAAAATG TCCTCAATTT TCCGATGCTT AAAACCACCG CGGTTTCGGA CGCTGGCCTG ACAAACCAGA CTCTATTCGA AGTGGAGCGT GGCCTGTTCA CGCGCTCGAC CTATGATCGA GCCTTGGAGG CGATGAACGC CGTCAACGAC GAGATCGAAA CACTGATCAA AAAAGCATGG GGTAGGCCCA CATGAGCCGG AAGCACATCC TTGGCGTCTC AACTGACGCC CCTGAGACGT CGCCCGCCGA CAATAGGACG GCAAAGAACC GCTCCATGCC GCTCCTCGGC GTAACAAGGA AGGAGCGCGA TCCGGCAACG AAGCTCACAG CGAACATTGG TAACGCACTG CGAGAGCAAA ACGATCGTCT TAGCCGTGCC GAAGAGATCG AGCGGCGTCT CGCTGAAGGT CAGGCAGTGA TAGAGTTGGA TGCCTCGTCA ATAGAACCGT CTTTCGTGCA GGATCGTATG CGAGGGGACA TTGACGGGCT CCTTACTTCG ATCCGGGAAC AAGGACAGCA AGTCCCAATC CTTGTGCGAC CGCATCCGAG CCAGCCGGGC CGATATCAGG TTGCCTTCGG CCACCGCCGG CTACGCGCCG TTTCAGAACT CGGACTTCCG GTCAGGGCGG TCGTTCGCGA ACTGACGGAC GAGCAAGTGG TCGTAGCACA GGGTCAGGAA AACAATGTGC GCGAAGATCT TACCTTCATC GAAAAGGCGC GCTTCGCACA TCGCCTGAAC AGGCAGTTTT CTCGAGAGAT TGTCATCGCC GCGATGTCGA TCGACAAGAG CAATTTGTCC AAGATGCTTC TGCTCGTTGA CGCCCTCCCC TCTGAACTGA CCGATGCTAT TGGTGCCGCT CCTGGTGTTG GACGGCCGAG TTGGCAACAA CTTGCCGAGC TGATTGAGAA AGTTTCTTCA CCGGCCGACG TGGCTAAATA TGCTATGTCG GAGGAAGTTC AAGCGCTGCC ATCGGCAGAA CGATTCAAGG CGGTGATCGC TAGTCTGAAG CCCAGTCGGG TTGCGCGTGG ACTTCCCGAG GTCATGGCCA CCCCAGACGG CACCAGAATT GCACAGGTGA CGCAGAGCAA GGCCAAACTG GAAATCACGA TTGACAGGAA GGCGACGCCC GATTTTGCGA CCTTCGTGCT CGATCATGTG CCAGCGCTGT ATCAAGCGTA CCACGCTGAG AACCAACGGA AACGGGGAGA GTAAACCGCA AAAGAAAAGA GCCCCCTCAA CGTCGCCGTC GCGGAAGCCC TTCTGTCTCT CTAGCGCGAA CAGAATCGCA TTTCCTCGAA TCCTCGTCAA GAGTTTTTAG CGCCGTTTTG GTGAGCTGAT TTCCTTTGCC TGCTGAAAGG TGAAAGATGA TGCAGACAGG AAGTGTAACG ACGCCATTCG GGCGGCGGCC AATGACGCTT GCGCTTGTGC GGCGCCAGAC GGCGCTGGCC GATATCAAAC AAGGCAAGAC AGCGGACAAG TGGAAGGTCT TTAGGGACGC GTCCGCGGCC ATGGAACTAC TTGGAATCCA GTCCAACAGT CTTGCCGTCC TTGATGCGCT ATTGAGCTTT CACCCGGAAA CGGAGTTGCG TCAGGAGGCA CAGCTGATCG TCTTCCCGTC GAATGCTCAG CTTGCCCTTC GGGCGCATGG GATGGCTGGC GCGACTTTGC GTAGGCACAT CGCCATGCTC GTGGAGTCAG GCTTGATCGT CCGGAAGGAT AGCGCCAACG GAAAGCGTTA CGCTCGTAAG GATGGCGCTG GTCAGATCGA GCGCGCGTTT GGCTTCGATT TGTCTCCGCT TCTCGCGCGG TCCGAAGAGC TAGCGATGAT GGCACAGCAG GTGATGGCCG ATCGAGCAGC ATTCAGGATG GCCAAAGAAA GTCTGACGAT TTGCCGACGG GACGTTCGGA AGCTAATTAC GGCAGCTATG GAAGAGGGAG CGGAGGGCGA CTGGCAAGCT GTCGAGGAAG TCTATGTGGA ACTTGTGGGT AGAATCCCAC GCGCCCCGAC GCTTGCTGAT GTAGAGTCAA TTCTCGAAGA GATGTGGATG CTCCAGGAAG AGATAATCAA CCGGTTGGAA ATTAGAGACA ATTCAGAAAA TAATAGCACC AATGCTGCCC AGAGCGAGCA GCACATACAG AACTCAAAAC CCGAATCCGT TAATGAACTT GAACCTCGCT CTGAAAAGGA GCAGGGCGCT AAGCCGAGTG AAATAGACCG GGCAAGGAGC GAGCCGATAA AAGCGTTCCC CCTCGGGATG ATCCTGAAAG CGTGCCCGAC CATTGGCAAT TATGGGCCGA GCGGTGCGGT TGCTAGCTGG CGTGACCTCA TGTCGGCTGC GGTGGTGGTT CGGTCTATGC TGGGGGTCAG CCCGTCGGCT TACCAAGACG CGTGTGAGGC AATGGGACCG GAGAATGCGG CAGCAGCGAT GGCGTGCATT TTGGAGCGAG CGAACTTCAT CAATTCGCCT GGGGGCTATC TCCGAGATCT GACACGGCGG AGCGAACTCG GGAAGTTTTC ACTTGGCCCG ATGATAATGG CGCTCTTGAA GGCTAGCGGG CAGGGGACGT TGCGGTTTGG CTAGAATTAG CGAGTATGGA GCAGGATGGT CTGTGGTCAG CTGACCACAG ACCTAATAGG TTGAAAACAT GAGCGTTTTT TGGATGATCG ACAGACCATC CGATTCCCGG AGTACCAAGC GTGCTCTGAT GGGAGCGATA ACATTACTCA ACAAGCACGA AGGCCCCATG CCGATCGTTG ATCGTGAAGG AGAGCCTGCT CTACATGCGG CGGTATTTTG CCGGCCGAGG CATGTAGTCG CGGAGCACTG CCTATTTACT GCCCTAGGCA CAAACGTTGA CTCTTGGATC GAGCTGGCAG ACAAAGCAAT AACCCACACA GAGGACGATT AATGGCTGAC GAAGAGATCC AGAATCCGCC GGACGGTACT GCTGCTGCCG AAGTTGAGCC GGCTGCTCCT AGAGGTAGAA GAGCAAAGAA AGCACCAGCC GAAACAGCCC GCACGGGATC GTTCAAATCC GTGAAGCCGA AAACCCGCGG CCTCAGCAAC CGAGAAAAAC TGGAGAAGAT CGGTCAAATC GAAGCTCAGG TCGCTGGCGG CGCAACCTTG AAGGACGCCG TTAAGATCGT GGGTATTTCC GTTCAGACCT ATTATCAATG GAAGAGAGCT GCGGTTCAAC CTGTCTCACA GAATCCGGCC GTGTCTGTTT CAGTTGACGA TGAACTCGGC GAGTTCATCC AACTCGAGGA GGAAAATCGG CGGCTCAGAA AGCTAGCTTG TGGCAGGATA TATTGTGGTG TAAACAAATT GACGCTTAGA CAACTTAATA ACACATTGCG GACGTTTTTA ATGTACTGGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGCCC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCAAATTC CCGATCTAGT AACATAGATG ACACCGCGCG CGATAATTTA TCCTAGTTTG CGCGCTATAT TTTGTTTTCT ATCGCGTATT AAATGTATAA TTGCGGGACT CTAATCATAA AAACCCATCT CATAAATAAC GTCATGCATT ACATGTTAAT TATTACATGC TTAACGTAAT TCAACAGAAA TTATATGATA ATCATCGCAA GACCGGCAAC AGGATTCAAT CTTAAGAAAC TTTATTGCCA AATGTTTGAA CGATCGGGGC CCGATCATAT TGTCGCTCAG GATCGTGGCG TTGTGCTTGT CGGCCGTTGC TGTCGTAATG ATATCGGCAC CTTCGACCGC CTGTTCCGCA GAGATCCCGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCATC GCCGTCGGGC ATACGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGTAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCAGATC CGGTGCAGAT TATTTGGATT GAGAGTGAAT ATGAGACTCT AATTGGATAC CGAGGGGAAT TTATGGAACG TCAGTGGAGC ATTTTTGACA AGAAATATTT GCTAGCTGAT AGTGACCTTA GGCGACTTTT GAACGCGCAA TAATGGTTTC TGACGTATGT GCTTAGCTCA TTAAACTCCA GAAACCCGCG GCTGAGTGGC TCCTTCAACG TTGCGGTTCT GTCAGTTCCA AACGTAAAAC GGCTTGTCCC GCGTCATCGG CGGGGGTCAT AACGTGACTC CCTTAATTCT CCGCTCATGA TCCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCCCGGGAT CCAGCGGCCG CAGGTACCAA TTCCTTATCT TTAATCATAT TCCATAGTCC ATACCATAGC ACATACAGTA GTTATATGCG AAGAGATCCA ACAAAACATT CACAATGGAT TATAGAAACA TTTGTTTATT CATTATAATG AGATCTTACA TTCATTTAAT ATTAGAAAAA GCCACAAATT CATAACACAA CAAGCCAAGA AAAAAACACA AACTTAAGCA CACAAGCTAG CTTTTTATTT GACACACCAA ATATTTCATC TTCATCTTCA TAAGAGCTCG GTAGCAATTC CCGAGGCTGT AGCCGACGAT GGTGCGCCAG GAGAGTTGTT GATTCAGAAT TGTCGACCCG GTAGTAACTT GCTAATCGAC GGGCATATGT AATCCCCCTC TTTTTCAAAG AACAAGAAAC CACTCCGTAG AGATCGAAAG CTCAGGTTTT CTTTTTTCTT GAATCACTCG CAGTTCGTTG CTTGGAATTC TCTAGAGTCC CCCGTGTTCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGGTCT TGCGAAGGAT AGTGGGATTG TGCGTCATCC CTTACGTCAG TGGAGATATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTCAA CGATGGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGA GCCACCTTCC TTTTCCACTA TCTTCACAAT AAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCGGATATTA CCCTTTGTTG AAAAGTCTCA ATTGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTTT TGGAGTAGAC AAGTGTGTCG TGCTCCACCA TGTTGACGAA GATTTTCTTC TTGTCATTGA GTCGTAAGAG ACTCTGTATG AACTGTTCGC CAGTCTTTAC GGCGAGTTCT GTTAGGTCCT CTATTTGAAT CTTTGACTCC ATGGCCTTTG ATTCAGTGGG AACTACCTTT TTAGAGACTC CAATCTCTAT TACTTGCCTT GGTTTGTGAA GCAAGCCTTG AATCGTCCAT ACTGGAATAG TACTTCTGAT CTTGAGAAAT ATATCTTTCT CTGTGTTCTT GATGCAGTTA GTCCTGAATC TTTTGACTGC ATCTTTAACC TTCTTGGGAA GGTATTTGAT TTCCTGGAGA TTATTGCTCG GGTAGATCGT CTTGATGAGA CCTGCTGCGT AAGCCTCTCT AACCATCTGT GGGTTAGCAT TCTTTCTGAA ATTGAAAAGG CTAATCTGGG GACCTGCAGG CATGCAAGCT TGGCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GGATCAGATT GTCGTTTCCC GCCTTCAGTT TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA TTAGAATAAT CGGATATTTA AAAGGGCGTG AAAAGGTTTA TCCGTTCGTC CATTTGTATG TGACTAAAAC AATTCATCCA GTAAAATATA ATATTTTATT TTCTCCCAAT CAGGCTTGAT CCCCAGTAAG TCAAAAAATA GCTCGACATA CTGTTCTTCC CCGATATCCT CCCTGATCGA CCGGACGCAG AAGGCAATGT CATACCACTT GTCCGCCCTG CCGCTTCTCC CAAGATCAAT AAAGCCACTT ACTTTGCCAT CTTTCACAAA GATGTTGCTG TCTCCCAGGT CGCCGTGGGA AAAGACAAGT TCCTCTTCGG GCTTTTCCGT CTTTAAAAAA TCATACAGCT CGCGCGGATC TTTAAATGGA GTGTCTTCTT CCCAGTTTTC GCAATCCACA TCGGCCAGAT CGTTATTCAG TAAGTAATCC AATTCGGCTA AGCGGCTGTC TAAGCTATTC GTATAGGGAC AATCCGATAT GTCGATGGAG TGAAAGAGCC TGATGCACTC CGCATACAGC TCGATAATCT TTTCAGGGCT TTGTTCATCT TCATACTCTT CCGAGCAAAG GACGCCATCG GCCTCACTCA TGAGCAGATT GCTCCAGCCA TCATGCCGTT CAAAGTGCAG GACCTTTGGA ACAGGCAGCT TTCCTTCCAG CCATAGCATC ATGTCCTTTT CCCGTTCCAC ATCATAGGTG GTCCCTTTAT ACCGGCTGTC CGTCATTTTT AAATATAGGT TTTCATTTTC TCCCACCAGC TTATATACCT TAGCAGGAGA CATTCCTTCC GTATCTTTTA CGCAGCGGTA TTTTTCGATC AGTTTTTTCA ATTCCGGTGA TATTCTCATT TTAGCCATTT ATTATTTCCT TCCTCTTTTC TACAGTATTT AAAGATACCC CAAGAAGCTA ATTATAACAA GACGAACTCC AATTCACTGT TCCTTGCATT CTAAAACCTT AAATACCAGA AAACAGCTTT TTCAAAGTTG TTTTCAAAGT TGGCGTATAA CATAGTATCG ACGGAGCCGA TTTTGAAACC ACAATTATGG GTGATGCTGC CAACTTACTG ATTTAGTGTA TGATGGTGTT TTTGAGGTGC TCCAGTGGCT TCTGTTTCTA TCAGCTGTCC CTCCTGTTCA GCTACTGACG GGGTGGTGCG TAACGGCAAA AGCACCGCCG GACATCAGCG CTATCTCTGC TCTCACTGCC GTAAAACATG GCAAC
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