pCX-DR
$128.00
In stock
SKU
PL80681
100μg liquid plasmid(Instantly):$149
| name | pCX-DR | another name | apCX-DR |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | GateWay |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCGAGC TCGGTACCCA CTGGATTTTG GTTTTAGGAA TTAGAAATTT TATTGATAGA AGTATTTTAC AAATACAAAT ACATACTAAG GGTTTCTTAT ATGCTCAACA CATGAGCGAA ACCCTATAGG AACCCTAATT CCCTTATCTG GGAACTACTC ACACATTATT ATGGAGAAAC TCGAAATTCG AGCTCCACCG CGGCTGAGTG GCTCCTTCAA CGTTGCGGTT CTGTCAGTTC CAAACGTAAA ACGGCTTGTC CCGCGTCATC GGCGGGGGTC ATAACGTGAC TCCCTTAATT CTCCGCTCAT GATCCGGGGA ATTAATTCCG ATCCTATCTG TCACTTCATC AAAAGGACAG TAGAAAAGGA AGGTGGCACC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACACGCTGAA ATCACCAGTC TCTCTCTACA AGATCCTCTA GCGCTACCGG TCGCCACCAT GGCCTCCTCC GAGAACGTCA TCACCGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCAC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCCA CAACACCGTG AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCCCAG TTCCAGTACG GCTCCAAGGT GTACGTGAAG CACCCCGCCG ACATCCCCGA CTACAAGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GCGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCTGCT TCATCTACAA GGTGAAGTTC ATCGGCGTGA ACTTCCCCTC CGACGGCCCC GTGATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG AGCGCCTGTA CCCCCGCGAC GGCGTGCTGA AGGGCGAGAC CCACAAGGCC CTGAAGCTGA AGGACGGCGG CCACTACCTG GTGGAGTTCA AGTCCATCTA CATGGCCAAG AAGCCCGTGC AGCTGCCCGG CTACTACTAC GTGGACGCCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAGCAGTAC GAGCGCACCG AGGGCCGCCA CCACCTGTTC CTGAGATCTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCC CCAATACTTG TATGGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCAGCAAAA GAGCCGTTCA TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT CATAGCATAC CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC CCATACAAGT ATTGGGGATC CACCGGATCT AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTGTGTGA ATTACAGGTG ACCAGCTCGA ATTTCCCCGA TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG GTCTTGCGAT GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA TGTAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG TGTCATCTAT GTTACTAGAT CGGGAATTAA ACTATCAGTG TTTGACAGGA TATATTGGCG GGTAAACCTA AGAGAAAAGA GCGTTTATTA GAATAACGGA TATTTAAAAG GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC TCGGGATCAA AGTACTTTGA TCCAACCCCT CCGCTGCTAT AGTGCAGTCG GCTTCTGACG TTCAGTGCAG CCGTCTTCTG AAAACGACAT GTCGCACAAG TCCTAAGTTA CGCGACAGGC TGCCGCCCTG CCCTTTTCCT GGCGTTTTCT TGTCGCGTGT TTTAGTCGCA TAAAGTAGAA TACTTGCGAC TAGAACCGGA GACATTACGC CATGAACAAG AGCGCCGCCG CTGGCCTGCT GGGCTATGCC CGCGTCAGCA CCGACGACCA GGACTTGACC AACCAACGGG CCGAACTGCA CGCGGCCGGC TGCACCAAGC TGTTTTCCGA GAAGATCACC GGCACCAGGC GCGACCGCCC GGAGCTGGCC AGGATGCTTG ACCACCTAGC CCTGGCGACG TTGTGACAGT GACCAGGCTA GACCGCCTGG CCCGCAGCAC CCGCGACCTA CTGGACATTG CCGAGCGCAT CCAGGAGGCC GGCGCGGGCC TGCGTAGCCT GGCAGAGCCG TGGGCCGACA CCACCACGCC GGCCGGCCGC ATGGTGTTGA CCGTGTTCGC CGGCATTGCC GAGTTCGAGC GTTCCCTAAT CATCGACCGC ACCCGGAGCG GGCGCGAGGC CGCCAAGGCC CGAGGCGTGA AGTTTGGCCC CCGCCCTACC CTCACCCCGG CACAGATCGC GCACGCCCGC GAGCTGATCG ACCAGGAAGG CCGCACCGTG AAAGAGGCGG CTGCACTGCT TGGCGTGCAT CGCTCGACCC TGTACCGCGC ACTTGAGCGC AGCGAGGAAG TGACGCCCAC CGAGGCCAGG CGGCGCGGTG CCTTCCGTGA GGACGCATTG ACCGAGGCCG ACGCCCTGGC GGCCGCCGAG AATGAACGCC AAGAGGAACA AGCATGAAAC CGCACCAGGA CGGCCAGGAC GAACCGTTTT TCATTACCGA AGAGATCGAG GCGGAGATGA TCGCGGCCGG GTACGTGTTC GAGCCGCCCG CGCACGTCTC AACCGTGCGG CTGCATGAAA TCCTGGCCGG TTTGTCTGAT GCCAAGCTGG CGGCCTGGCC GGCCAGCTTG GCCGCTGAAG AAACCGAGCG CCGCCGTCTA AAAAGGTGAT GTGTATTTGA GTAAAACAGC TTGCGTCATG CGGTCGCTGC GTATATGATG CGATGAGTAA ATAAACAAAT ACGCAAGGGG AACGCATGAA GGTTATCGCT GTACTTAACC AGAAAGGCGG GTCAGGCAAG ACGACCATCG CAACCCATCT AGCCCGCGCC CTGCAACTCG CCGGGGCCGA TGTTCTGTTA GTCGATTCCG ATCCCCAGGG CAGTGCCCGC GATTGGGCGG CCGTGCGGGA AGATCAACCG CTAACCGTTG TCGGCATCGA CCGCCCGACG ATTGACCGCG ACGTGAAGGC CATCGGCCGG CGCGACTTCG TAGTGATCGA CGGAGCGCCC CAGGCGGCGG ACTTGGCTGT GTCCGCGATC AAGGCAGCCG ACTTCGTGCT GATTCCGGTG CAGCCAAGCC CTTACGACAT ATGGGCAACC GCCGACCTGG TGGAGCTGGT TAAGCAGCGC ATTGAGGTCA CGGATGGAAG GCTACAAGCG GCCTTTGTCG TGTCGCGGGC GATCAAAGGC ACGCGCATCG GCGGTGAGGT TGCCGAGGCG CTGGCCGGGT ACGAGCTGCC CATTCTTGAG TCCCGTATCA CGCAGCGCGT GAGCTACCCA GGCACTGCCG CCGCCGGCAC AACCGTTCTT GAATCAGAAC CCGAGGGCGA CGCTGCCCGC GAGGTCCAGG CGCTGGCCGC TGAAATTAAA TCAAAACTCA TTTGAGTTAA TGAGGTAAAG AGAAAATGAG CAAAAGCACA AACACGCTAA GTGCCGGCCG TCCGAGCGCA CGCAGCAGCA AGGCTGCAAC GTTGGCCAGC CTGGCAGACA CGCCAGCCAT GAAGCGGGTC AACTTTCAGT TGCCGGCGGA GGATCACACC AAGCTGAAGA TGTACGCGGT ACGCCAAGGC AAGACCATTA CCGAGCTGCT ATCTGAATAC ATCGCGCAGC TACCAGAGTA AATGAGCAAA TGAATAAATG AGTAGATGAA TTTTAGCGGC TAAAGGAGGC GGCATGGAAA ATCAAGAACA ACCAGGCACC GACGCCGTGG AATGCCCCAT GTGTGGAGGA ACGGGCGGTT GGCCAGGCGT AAGCGGCTGG GTTGTCTGCC GGCCCTGCAA TGGCACTGGA ACCCCCAAGC CCGAGGAATC GGCGTGACGG TCGCAAACCA TCCGGCCCGG TACAAATCGG CGCGGCGCTG GGTGATGACC TGGTGGAGAA GTTGAAGGCC GCGCAGGCCG CCCAGCGGCA ACGCATCGAG GCAGAAGCAC GCCCCGGTGA ATCGTGGCAA GCGGCCGCTG ATCGAATCCG CAAAGAATCC CGGCAACCGC CGGCAGCCGG TGCGCCGTCG ATTAGGAAGC CGCCCAAGGG CGACGAGCAA CCAGATTTTT TCGTTCCGAT GCTCTATGAC GTGGGCACCC GCGATAGTCG CAGCATCATG GACGTGGCCG TTTTCCGTCT GTCGAAGCGT GACCGACGAG CTGGCGAGGT GATCCGCTAC GAGCTTCCAG ACGGGCACGT AGAGGTTTCC GCAGGGCCGG CCGGCATGGC CAGTGTGTGG GATTACGACC TGGTACTGAT GGCGGTTTCC CATCTAACCG AATCCATGAA CCGATACCGG GAAGGGAAGG GAGACAAGCC CGGCCGCGTG TTCCGTCCAC ACGTTGCGGA CGTACTCAAG TTCTGCCGGC GAGCCGATGG CGGAAAGCAG AAAGACGACC TGGTAGAAAC CTGCATTCGG TTAAACACCA CGCACGTTGC CATGCAGCGT ACGAAGAAGG CCAAGAACGG CCGCCTGGTG ACGGTATCCG AGGGTGAAGC CTTGATTAGC CGCTACAAGA TCGTAAAGAG CGAAACCGGG CGGCCGGAGT ACATCGAGAT CGAGCTAGCT GATTGGATGT ACCGCGAGAT CACAGAAGGC AAGAACCCGG ACGTGCTGAC GGTTCACCCC GATTACTTTT TGATCGATCC CGGCATCGGC CGTTTTCTCT ACCGCCTGGC ACGCCGCGCC GCAGGCAAGG CAGAAGCCAG ATGGTTGTTC AAGACGATCT ACGAACGCAG TGGCAGCGCC GGAGAGTTCA AGAAGTTCTG TTTCACCGTG CGCAAGCTGA TCGGGTCAAA TGACCTGCCG GAGTACGATT TGAAGGAGGA GGCGGGGCAG GCTGGCCCGA TCCTAGTCAT GCGCTACCGC AACCTGATCG AGGGCGAAGC ATCCGCCGGT TCCTAATGTA CGGAGCAGAT GCTAGGGCAA ATTGCCCTAG CAGGGGAAAA AGGTCGAAAA GGTCTCTTTC CTGTGGATAG CACGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG AACCCGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGTCACACAT GTAAGTGACT GATATAAAAG AGAAAAAAGG CGATTTTTCC GCCTAAAACT CTTTAAAACT TATTAAAACT CTTAAAACCC GCCTGGCCTG TGCATAACTG TCTGGCCAGC GCACAGCCGA AGAGCTGCAA AAAGCGCCTA CCCTTCGGTC GCTGCGCTCC CTACGCCCCG CCGCTTCGCG TCGGCCTATC GCGGCCGCTG GCCGCTCAAA AATGGCTGGC CTACGGCCAG GCAATCTACC AGGGCGCGGA CAAGCCGCGC CGTCGCCACT CGACCGCCGG CGCCCACATC AAGGCACCCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTTGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGAAT TAACGCCGAA TTAATTCGGG GGATCTGGAT TTTAGTACTG GATTTTGGTT TTAGGAATTA GAAATTTTAT TGATAGAAGT ATTTTACAAA TACAAATACA TACTAAGGGT TTCTTATATG CTCAACACAT GAGCGAAACC CTATAGGAAC CCTAATTCCC TTATCTGGGA ACTACTCACA CATTATTATG GAGAAACTCG AGCTTGTCGA TCGACAGATC CGGTCGGCAT CTACTCTATT TCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG GAGCATATAC GCCCGGAGTC GTGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCACG AGGTGCCGGA CTTCGGGGCA GTCCTCGGCC CAAAGCATCA GCTCATCGAG AGCCTGCGCG ACGGACGCAC TGACGGTGTC GTCCATCACA GTTTGCCAGT GATACACATG GGGATCAGCA ATCGCGCATA TGAAATCACG CCATGTAGTG TATTGACCGA TTCCTTGCGG TCCGAATGGG CCGAACCCGC TCGTCTGGCT AAGATCGGCC GCAGCGATCG CATCCATAGC CTCCGCGACC GGTTGTAGAA CAGCGGGCAG TTCGGTTTCA GGCAGGTCTT GCAACGTGAC ACCCTGTGCA CGGCGGGAGA TGCAATAGGT CAGGCTCTCG CTAAACTCCC CAATGTCAAG CACTTCCGGA ATCGGGAGCG CGGCCGATGC AAAGTGCCGA TAAACATAAC GATCTTTGTA GAAACCATCG GCGCAGCTAT TTACCCGCAG GACATATCCA CGCCCTCCTA CATCGAAGCT GAAAGCACGA GATTCTTCGC CCTCCGAGAG CTGCATCAGG TCGGAGACGC TGTCGAACTT TTCGATCAGA AACTTCTCGA CAGACGTCGC GGTGAGTTCA GGCTTTTTCA TATCTCATTG CCCCCCGGAT CTGCGAAAGC TCGAGAGAGA TAGATTTGTA GAGAGAGACT GGTGATTTCA GCGTGTCCTC TCCAAATGAA ATGAACTTCC TTATATAGAG GAAGGTCTTG CGAAGGATAG TGGGATTGTG CGTCATCCCT TACGTCAGTG GAGATATCAC ATCAATCCAC TTGCTTTGAA GACGTGGTTG GAACGTCTTC TTTTTCCACG ATGCTCCTCG TGGGTGGGGG TCCATCTTTG GGACCACTGT CGGCAGAGGC ATCTTGAACG ATAGCCTTTC CTTTATCGCA ATGATGGCAT TTGTAGGTGC CACCTTCCTT TTCTACTGTC CTTTTGATGA AGTGACAGAT AGCTGGGCAA TGGAATCCGA GGAGGTTTCC CGATATTACC CTTTGTTGAA AAGTCTCAAT AGCCCTTTGG TCTTCTGAGA CTGTATCTTT GATATTCTTG GAGTAGACGA GAGTGTCGTG CTCCACCATG TTATCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG GCAAGCTGCT CTAGCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTAC
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