pGilda
$99.00
In stock
SKU
PL80633
100μg liquid plasmid(Instantly):$120
| name | pGilda | another name | apGilda |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | HIS3 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTGAATTTT CAAAAATTCT TACTTTTTTT TTGGATGGAC GCAAAGAAGT TTAATAATCA TATTACATGG CATTACCACC ATATACATAT CCATATACAT ATCCATATCT AATCTTACTT ATATGTTGTG GAAATGTAAA GAGCCCCATT ATCTTAGCCT AAAAAAACCT TCTCTTTGGA ACTTTCAGTA ATACGCTTAA CTGCTCATTG CTATATTGAA GTACGGATTA GAAGCCGCCG AGCGGGTGAC AGCCCTCCGA AGGAAGACTC TCCTCCGTGC GTCCTCGTCT TCACCGGTCG CGTTCCTGAA ACGCAGATGT GCCTCGCGCC GCACTGCTCC GAACAATAAA GATTCTACAA TACTAGCTTT TATGGTTATG AAGAGGAAAA ATTGGCAGTA ACCTGGCCCC ACAAACCTTC AAATGAACGA ATCAAATTAA CAACCATAGG ATGATAATGC GATTAGTTTT TTAGCCTTAT TTCTGGGGTA ATTAATCAGC GAAGCGATGA TTTTTGATCT ATTAACAGAT ATATAAATGC AAAAACTGCA TAACCACTTT AACTAATACT TTCAACATTT TCGGTTTGTA TTACTTCTTA TTCAAATGTA ATAAAAGTAT CAACAAAAAA TTGTTAATAT ACCTCTATAC TTTAACGTCA AGGAGAAAAA ACCCCGGATC AAGGGTGCGA TATGAAAGCG TTAACGGCCA GGCAACAAGA GGTGTTTGAT CTCATCCGTG ATCACATCAG CCAGACAGGT ATGCCGCCGA CGCGTGCGGA AATCGCGCAG CGTTTGGGGT TCCGTTCCCC AAACGCGGCT GAAGAACATC TGAAGGCGCT GGCACGCAAA GGCGTTATTG AAATTGTTTC CGGCGCATCA CGCGGGATTC GTCTGTTGCA GGAAGAGGAA GAAGGGTTGC CGCTGGTAGG TCGTGTGGCT GCCGGTGAAC CACTTCTGGC GCAACAGCAT ATTGAAGGTC ATTATCAGGT CGATCCTTCC TTATTCAAGC CGAATGCTGA TTTCCTGCTG CGCGTCAGCG GGATGTCGAT GAAAGATATC GGCATTATGG ATGGTGACTT GCTGGCAGTG CATAAAACTC AGGATGTACG TAACGGTCAG GTCGTTGTCG CACGTATTGA TGACGAAGTT ACCGTTAAGC GCCTGAAAAA ACAGGGCAAT AAAGTCGAAC TGTTGCCAGA AAATAGCGAG TTTAAACCAA TTGTCGTAGA TCTTCGTCAG CAGAGCTTCA CCATTGAAGG GCTGGCGGTT GGGGTTATTC GCAACGGCGA CTGGCTGGAA TTCCCGGGGA TCCGTCGACC ATGGCGGCCG CTCGAGTCGA CCTGCAGCCA AGCTAATTCC GGGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA ATTCTTGTTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGCATGA GGTCGCTCTT ATTGACCACA CCTCTACCGG CATGCCGAGC AAATGCCTGC AAATCGCTCC CCATTTCACC CAATTGTAGA TATGCTAACT CCAGCAATGA GTTGATGAAT CTCGGTGTGT ATTTTATGTC CTCAGAGGAC AACACCTGTT GTAATCCGTC CGAGCTCCAA TTCGCCCTAT AGTGAGTCGT ATTACAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GATCCGTCGA GTTCAAGAGA AAAAAAAAGA AAAAGCAAAA AGAAAAAAGG AAAGCGCGCC TCGTTCAGAA TGACACGTAT AGAATGATGC ATTACCTTGT CATCTTCAGT ATCATACTGT TCGTATACAT ACTTACTGAC ATTCATAGGT ATACATATAT ACACATGTAT ATATATCGTA TGCTGCAGCT TTAAATAATC GGTGTCACTA CATAAGAACA CCTTTGGTGG AGGGAACATC GTTGGTACCA TTGGGCGAGG TGGCTTCTCT TATGGCAACC GCAAGAGCCT TGAACGCACT CTCACTACGG TGATGATCAT TCTTGCCTCG CAGACAATCA ACGTGGAGGG TAATTCTGCT AGCCTCTGCA AAGCTTTCAA GAAAATGCGG GATCATCTCG CAAGAGAGAT CTCCTACTTT CTCCCTTTGC AAACCAAGTT CGACAACTGC GTACGGCCTG TTCGAAAGAT CTACCACCGC TCTGGAAAGT GCCTCATCCA AAGGCGCAAA TCCTGATCCA AACCTTTTTA CTCCACGCGC CAGTAGGGCC TCTTTAAAAG CTTGACCGAG AGCAATCCCG CAGTCTTCAG TGGTGTGATG GTCGTCTATG TGTAAGTCAC CAATGCACTC AACGATTAGC GACCAGCCGG AATGCTTGGC CAGAGCATGT ATCATATGGT CCAGAAACCC TATACCTGTG TGGACGTTAA TCACTTGCGA TTGTGTGGCC TGTTCTGCTA CTGCTTCTGC CTCTTTTTCT GGGAAGATCG AGTGCTCTAT CGCTAGGGGA CCACCCTTTA AAGAGATCGC AATCTGAATC TTGGTTTCAT TTGTAATACG CTTTACTAGG GCTTTCTGCT CTGTCATCTT TGCCTTCGTT TATCTTGCCT GCTCATTTTT TAGTATATTC TTCGAAGAAA TCACATTACT TTATATAATG TATAATTCAT TATGTGATAA TGCCAATCGC TAAGAAAAAA AAAGAGTCAT CCGCTAGGTG GAAAAAAAAA AATGAAAATC ATTACCGAGG CATAAAAAAA TATAGAGTGT ACTAGAGGAG GCCAAGAGTA ATAGAAAAAG AAAATTGCGG GAAAGGACTG TGTTATGACT TCCCTGACTA ATGCCGTGTT CAAACGATAC CTGGCAGTGA CTCCTAGCGC TCACCAAGCT CTTAAAACGG AATTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGGACGGA TCGCTTGCCT GTAACTTACA CGCGCCTCGT ATCTTTTAAT GATGGAATAA TTTGGGAATT TACTCTGTGT TTATTTATTT TTATGTTTTG TATTTGGATT TTAGAAAGTA AATAAAGAAG GTAGAAGAGT TACGGAATGA AGAAAAAAAA ATAAACAAAG GTTTAAAAAA TTTCAACAAA AAGCGTACTT TACATATATA TTTATTAGAC AAGAAAAGCA GATTAAATAG ATATACATTC GATTAACGAT AAGTAAAATG TAAAATCACA GGATTTTCGT GTGTGGTCTT CTACACAGAC AAGATGAAAC AATTCGGCAT TAATACCTGA GAGCAGGAAG AGCAAGATAA AAGGTAGTAT TTGTTGGCGA TCCCCCTAGA GTCTTTTACA TCTTCGGAAA ACAAAAACTA TTTTTTCTTT AATTTCTTTT TTTACTTTCT ATTTTTAATT TATATATTTA TATTAAAAAA TTTAAATTAT AATTATTTTT ATAGCACGTG ATGAAAAGGA CCCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTTCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GCAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGGAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCC GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACCCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC CGGGCCCCCC CTCGAAATTC
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