pFastBac1-Gus
$99.00
In stock
SKU
PL80625
100μg liquid plasmid(Instantly):$120
| name | pFastBac1-Gus | another name | apFastBac1-Gus |
| Insert Size (bp) | 1812bp | Plasmid host | Insect cells |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Gus | |
| Promoter | PH | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pFastBac1 | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCAGACCAGC CGCGTAACCT GGCAAAATCG GTTACGGTTG AGTAATAAAT GGATGCCCTG CGTAAGCGGG TGTGGGCGGA CAATAAAGTC TTAAACTGAA CAAAATAGAT CTAAACTATG ACAATAAAGT CTTAAACTAG ACAGAATAGT TGTAAACTGA AATCAGTCCA GTTATGCTGT GAAAAAGCAT ACTGGACTTT TGTTATGGCT AAAGCAAACT CTTCATTTTC TGAAGTGCAA ATTGCCCGTC GTATTAAAGA GGGGCGTGGC CAAGGGCATG GTAAAGACTA TATTCGCGGC GTTGTGACAA TTTACCGAAC AACTCCGCGG CCGGGAAGCC GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA AGTGCATCAC TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC CGTAATCTGC TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA GATTGATGAG CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT AGATATAGAT CTCACTACGC GGCTGCTCAA ACCTGGGCAG AACGTAAGCC GCGAGAGCGC CAACAACCGC TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT TCCCGAGGTA ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC GACCGAAAAG ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG TGCGAATGAT GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG TAACATCGTT GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA CAAGCCATGA AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC CAGTTGCGTG AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC AACTGGGTTC GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC AGCGAAGTCG AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG CATCGTCAGG CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG CCCTGGCTTC AGGAGATCGG AAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC CCGGATGAAG TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG GACTCTAGCT ATAGTTCTAG TGGTTGGCTA CGTATACTCC GGAATATTAA TAGGATCATG GAGATAATTA AAATGATAAC CATCTCGCAA ATAAATAAGT ATTTTACTGT TTTCGTAACA GTTTTGTAAT AAAAAAACCT ATAAATATTC CGGATTATTC ATACCGTCCC ACCATCGGGC GCGGATCCAT ATATAGGGCC CGGGTTATAA TTACCTCAGG TCGACGTCCC ATGGTCCGTC CTGTAGAAAC CCCAACCCGT GAAATCAAAA AACTCGACGG CCTGTGGGCA TTCAGTCTGG ATCGCGAAAA CTGTGGAATT GATCAGCGTT GGTGGGAAAG CGCGTTACAA GAAAGCCGGG CAATTGCTGT GCCAGGCAGT TTTAACGATC AGTTCGCCGA TGCAGATATT CGTAATTATG CGGGCAACGT CTGGTATCAG CGCGAAGTCT TTATACCGAA AGGTTGGGCA GGCCAGCGTA TCGTGCTGCG TTTCGATGCG GTCACTCATT ACGGCAAAGT GTGGGTCAAT AATCAGGAAG TGATGGAGCA TCAGGGCGGC TATACGCCAT TTGAAGCCGA TGTCACGCCG TATGTTATTG CCGGGAAAAG TGTACGTATC ACCGTTTGTG TGAACAACGA ACTGAACTGG CAGACTATCC CGCCGGGAAT GGTGATTACC GACGAAAACG GCAAGAAAAA GCAGTCTTAC TTCCATGATT TCTTTAACTA TGCCGGAATC CATCGCAGCG TAATGCTCTA CACCACGCCG AACACCTGGG TGGACGATAT CACCGTGGTG ACGCATGTCG CGCAAGACTG TAACCACGCG TCTGTTGACT GGCAGGTGGT GGCCAATGGT GATGTCAGCG TTGAACTGCG TGATGCGGAT CAACAGGTGG TTGCAACTGG ACAAGGCACT AGCGGGACTT TGCAAGTGGT GAATCCGCAC CTCTGGCAAC CGGGTGAAGG TTATCTCTAT GAACTGTGCG TCACAGCCAA AAGCCAGACA GAGTGTGATA TCTACCCGCT TCGCGTCGGC ATCCGGTCAG TGGCAGTGAA GGGCGAACAG TTCCTGATTA ACCACAAACC GTTCTACTTT ACTGGCTTTG GTCGTCATGA AGATGCGGAC TTACGTGGCA AAGGATTCGA TAACGTGCTG ATGGTGCACG ACCACGCATT AATGGACTGG ATTGGGGCCA ACTCCTACCG TACCTCGCAT TACCCTTACG CTGAAGAGAT GCTCGACTGG GCAGATGAAC ATGGCATCGT GGTGATTGAT GAAACTGCTG CTGTCGGCTT TAACCTCTCT TTAGGCATTG GTTTCGAAGC GGGCAACAAG CCGAAAGAAC TGTACAGCGA AGAGGCAGTC AACGGGGAAA CTCAGCAAGC GCACTTACAG GCGATTAAAG AGCTGATAGC GCGTGACAAA AACCACCCAA GCGTGGTGAT GTGGAGTATT GCCAACGAAC CGGATACCCG TCCGCAAGGT GCACGGGAAT ATTTCGCGCC ACTGGCGGAA GCAACGCGTA AACTCGACCC GACGCGTCCG ATCACCTGCG TCAATGTAAT GTTCTGCGAC GCTCACACCG ATACCATCAG CGATCTCTTT GATGTGCTGT GCCTGAACCG TTATTACGGA TGGTATGTCC AAAGCGGCGA TTTGGAAACG GCAGAGAAGG TACTGGAAAA AGAACTTCTG GCCTGGCAGG AGAAACTGCA TCAGCCGATT ATCATCACCG AATACGGCGT GGATACGTTA GCCGGGCTGC ACTCAATGTA CACCGACATG TGGAGTGAAG AGTATCAGTG TGCATGGCTG GATATGTATC ACCGCGTCTT TGATCGCGTC AGCGCCGTCG TCGGTGAACA GGTATGGAAT TTCGCCGATT TTGCGACCTC GCAAGGCATA TTGCGCGTTG GCGGTAACAA GAAAGGGATC TTCACTCGCG ACCGCAAACC GAAGTCGGCG GCTTTTCTGC TGCAAAAACG CTGGACTGGC ATGAACTTCG GTGAAAAACC GCAGCAGGGA GGCAAACAAT GAATCAACAA CTCTCCTGGC GCACCATCGT CGGCTACAGC CTCGGTGGGG AATTCAAAGG CCTACGTCGA CGAGCTCACT AGTCGCGGCC GCTTTCGAAT CTAGAGCCTG CAGTCTCGAG GCATGCGGTA CCAAGCTTGT CGAGAAGTAC TAGAGGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGAT CACTGCTTGA GCCTAGGAGA TCCGAACCAG ATAAGTGAAA TCTAGTTCCA AACTATTTTG TCATTTTTAA TTTTCGTATT AGCTTACGAC GCTACACCCA GTTCCCATCT ATTTTGTCAC TCTTCCCTAA ATAATCCTTA AAAACTCCAT TTCCACCCCT CCCAGTTCCC AACTATTTTG TCCGCCCACA GCGGGGCATT TTTCTTCCTG TTATGTTTTT AATCAAACAT CCTGCCAACT CCATGTGACA AACCGTCATC TTCGGCTACT TTTTCTCTGT CACAGAATGA AAATTTTTCT GTCATCTCTT CGTTATTAAT GTTTGTAATT GACTGAATAT CAACGCTTAT TTGCAGCCTG AATGGCGAAT G
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