pRE112
$99.00
In stock
SKU
PL80621
100μg liquid plasmid(Instantly):$134
| name | pRE112 | another name | apRE112 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | R6K | |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCGTTTCCG AGAACCGCGC GAACGACATG GAGCGGCACG CGGGCGTGGA AAGCCTGGTC GGCTGGATCG GCACGATGCG TCCGGCGTAG AGGATCTGAA GATCCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTCAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CTGATCCTTT TTAACCCATC ACATATACCT GCCGTTCACT ATTATTTAGT GAAATGAGAT ATTATGATAT TTTCTGAATT GTGATTAAAA AGGCAACTTT ATGCCCATGC AACAGAAACT ATAAAAAATA CAGAGAATGA AAAGAAACAG ATAGATTTTT TAGTTCTTTA GGCCCGTAGT CTGCAAATCC TTTTATGATT TTCTATCAAA CAAAAGAGGA AAATAGACCA GTTGCAATCC AAACGAGAGT CTAATAGAAT GAGGTCGAAA AGTAAATCGC GCGGGTTTGT TACTGATAAA GCAGGCAAGA CCTAAAATGT GTAAAGGGCA AAGTGTATAC TTTGGCGTCA CCCCTTACAT ATTTTAGGTC TTTTTTTATT GTGCGTAACT AACTTGCCAT CTTCAAACAG GAGGGCTGGA AGAAGCAGAC CGCTAACACA GTACATAAAA AAGGAGACAT GAACGATGAA CATCAAAAAG TTTGCAAAAC AAGCAACAGT ATTAACCTTT ACTACCGCAC TGCTGGCAGG AGGCGCAACT CAAGCGTTTG CGAAAGAAAC GAACCAAAAG CCATATAAGG AAACATACGG CATTTCCCAT ATTACACGCC ATGATATGCT GCAAATCCCT GAACAGCAAA AAAATGAAAA ATATCAAGTT CCTGAGTTCG ATTCGTCCAC AATTAAAAAT ATCTCTTCTG CAAAAGGCCT GGACGTTTGG GACAGCTGGC CATTACAAAA CGCTGACGGC ACTGTCGCAA ACTATCACGG CTACCACATC GTCTTTGCAT TAGCCGGAGA TCCTAAAAAT GCGGATGACA CATCGATTTA CATGTTCTAT CAAAAAGTCG GCGAAACTTC TATTGACAGC TGGAAAAACG CTGGCCGCGT CTTTAAAGAC AGCGACAAAT TCGATGCAAA TGATTCTATC CTAAAAGACC AAACACAAGA ATGGTCAGGT TCAGCCACAT TTACATCTGA CGGAAAAATC CGTTTATTCT ACACTGATTT CTCCGGTAAA CATTACGGCA AACAAACACT GACAACTGCA CAAGTTAACG TATCAGCATC AGACAGCTCT TTGAACATCA ACGGTGTAGA GGATTATAAA TCAATCTTTG ACGGTGACGG AAAAACGTAT CAAAATGTAC AGCAGTTCAT CGATGAAGGC AACTACAGCT CAGGCGACAA CCATACGCTG AGAGATCCTC ACTACGTAGA AGATAAAGGC CACAAATACT TAGTATTTGA AGCAAACACT GGAACTGAAG ATGGCTACCA AGGCGAAGAA TCTTTATTTA ACAAAGCATA CTATGGCAAA AGCACATCAT TCTTCCGTCA AGAAAGTCAA AAACTTCTGC AAAGCGATAA AAAACGCACG GCTGAGTTAG CAAACGGCGC TCTCGGTATG ATTGAGCTAA ACGATGATTA CACACTGAAA AAAGTGATGA AACCGCTGAT TGCATCTAAC ACAGTAACAG ATGAAATTGA ACGCGCGAAC GTCTTTAAAA TGAACGGCAA ATGGTATCTG TTCACTGACT CCCGCGGATC AAAAATGACG ATTGACGGCA TTACGTCTAA CGATATTTAC ATGCTTGGTT ATGTTTCTAA TTCTTTAACT GGCCCATACA AGCCGCTGAA CAAAACTGGC CTTGTGTTAA AAATGGATCT TGATCCTAAC GATGTAACCT TTACTTACTC ACACTTCGCT GTACCTCAAG CGAAAGGAAA CAATGTCGTG ATTACAAGCT ATATGACAAA CAGAGGATTC TACGCAGACA AACAATCAAC GTTTGCGCCC AGCTTCCTGC TGAACATCAA AGGCAAGAAA ACATCTGTTG TCAAAGACAG CATCCTTGAA CAAGGACAAT TAACAGTTAA CAAATAAAAA CGCAAAAGAA AATGCCGATA TCCTATTGGC ATTTTCTTTT ATTTCTTATC AACATAAAGG TGAATCCCAT ATGAACTATA TAAAAGCAGG CAAATGGCTA ACCGTATTCC TAACCTTTTG GTAATGACTC CAACTTATTG ATAGTGTTTT ATGTTCAGAT AATGCCCGAT GACTTTGTCA TGCAGCTCCA CCGATTTTGA GAACGACAGC GACTTCCGTC CCAGCCGTGC CAGGTGCTGC CTCAGATTCA GGTTATGCCG CTCAATTCGC TGCGTATATC GCTTGCTGAT TACGTGCAGC TTTCCCTTCA GGCGGGATTC ATACAGCGGC CAGCCATCCG TCATCCATAT CACCACGTCA AAGGGTGACA GCAGGCTCAT AAGACGCCCC AGCGTCGCCA TAGTGCGTTC ACCGAATACG TGCGCAACAA CCGTCTTCCG GAGACTGTCA TACGCGTAAA ACAGCCAGCG CTGGCGCGAT TTAGCCCCGA CATAGCCCCA CTGTTCGTCC ATTTCCGCGC AGACGATGAC GTCACTGCCC GGCTGTATGC GCGAGGTTAC CGACTGCGGC CTGAGTTTTT TAAGTGACGT AAAATCGTGT TGAGGCCAAC GCCCATAATG CGGGCTGTTG CCCGGCATCC AACGCCATTC ATGGCCATAT CAATGATTTT CTGGTGCGTA CCGGGTTGAG AAGCGGTGTA AGTGAACTGC ATGAATTCCC GGGAGAGCTC GATATCGCAT GCGGTACCTC TAGAAGAAGC TTGGGATCGG GCCCTATCAC TTATTCAGGC GTAGCAACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC AGGTATTAGG GCCCGATCCT TTTTGTCCGG TGTTGGGTTG AAGGTGAAGC CGGTCGGGGC CGCAGCGGGG GCCGGCTTTT CAGCCTTGCC CCCCTGCTTC GGCCGCCGTG GCTCCGGCGT CTTGGGTGCC GGCGCGGGTT CCGCAGCCTT GGCCTGCGGT GCGGGCACAT CGGCGGGCTT GGCCTTGATG TGCCGCCTGG CGTGCGAGCG GAACGTCTCG TAGGAGAACT TGACCTTCCC CGTTTCCCGC ATGTGCTCCC AAATGGTGAC GAGCGCATAG CCGGACGCTA ACGCCGCCTC GACATCCGCC CTCACCGCCA GGAACGCAAC CGCAGCCTCA TCACGCCGGC GCTTCTTGGC CGCGCGGGAT TCAACCCACT CGGCCAGCTC GTCGGTGTAG CTCTTTGGCA TCGTCTCTCG CCTGTCCCCT CAGTTCAGTA ATTTCCTGCA TTTGCCTGTT TCCAGTCGGT AGATATTCCA CAAAACAGCA GGGAAGCAGC GCTTTTCCGC TGCATAACCC TGCTTCGGGG TCATTATAGC GATTTTTTCG GTATATCCAT CCTTTTTCGC ACGATATACA GGATTTTGCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA TCCAACGGCG TCAGCCGGGC AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT TCTTCACTGT CCCTTATTCG CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT GGCCGGCTAC CGCCGGCGTA ACAGATGAGG GCAAGCGGAT GGCTGATGAA ACCAAGCCAA CCAGGAAGGG CAGCCCACCT ATCAAGGTGT ACTGCCTTCC AGACGAACGA AGAGCGATTG AGGAAAAGGC GGCGGCGGCC GGCATGAGCC TGTCGGCCTA CCTGCTGGCC GTCGGCCAGG GCTACAAAAT CACGGGCGTC GTGGACTATG AGCACGTCCG CGAGCTGGCC CGCATCAATG GCGACCTGGG CCGCCTGGGC GGCCTGCTGA AACTCTGGCT CACCGACGAC CCGCGCACGG CGCGGTTCGG TGATGCCACG ATCCTCGCCC TGCTGGCGAA GATCGAAGAG AAGCAGGACG AGCTTGGCAA GGTCATGATG GGCGTGGTCC GCCCGAGGGC AGAGCCATGA CTTTTTTAGC CGCTAAAACG GCCGGGGGGT GCGCGTGATT GCCAAGCACG TCCCCATGCG CTCCATCAAG AAGAGCGACT TCGCGGAGCT GGTGAAGTAC ATCACCGACG AGCAAGGCAA GACCGAGCGC CTGGGTCACG TGCGCGTCAC GAACTGCGAG GCAAACACCC TGCCCGCTGT CATGGCCGAG GTGATGGCGA CCCAGCACGG CAACACCCGT TCCGAGGCCG ACAAGACCTA TCACCTGCTG GTTAGCTTCC GCGCGGGAGA GAAGCCCGAC GCGGAGACGT TGCGCGCGAT TGAGGACCGC ATCTGCGCTG GGCTTGGCTT CGCCGAGCAT CAGCGCGTCA GTGCCGTGCA TCACGACACC GACAACCTGC ACATCCATAT CGCCATCAAC AAGATTCACC CGACCCGAAA CACCATCCAT GAGCCGTATC GGGCCTACCG CGCCCTCGCT GACCTCTGCG CGACGCTCGA ACGGGACTAC GGGCTTGAGC GTGACAATCA CGAAACGCGG CAGCGCGTTT CCGAGAACCG
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