pDUAL-HFG1
$99.00
In stock
SKU
PL80490
100μg liquid plasmid(Instantly):$120
| name | pDUAL-HFG1 | another name | apDUAL-HFG1 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | nmt | Replicon | ars1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGCC CTGAAATTGT TGCTTCTGCC TTGGAGGTTT TGAAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTTGAAGAA CACAAGATTG GAGGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCA ATGTCTGCGA ATTTGCGATC CTCAAAGATA AGAAAACGAT GCTTTTTACC TAAGGCCACC AGTTTTTCTA CCATATCCTG GTCGAAATCC TCGACAACGT CAATATGTGT CTTGATAACA CAGACATAGG GTCCAATTTT ATCTACCAAT TCTAAGATTT CGGATTTCTT CGTCAAATCG ACCGCGACTG ACAAGTTGCT TTGCTTTTCT TCCATCAAAG CCAACAATTC CTTGGCAATG GGATTTTTCA TCCCCTCAGC TCTAGCTGAA TAGCTTTGAA ATACTCTAGC ATCCATAACT TTGCTTTTAA ACCTTTAATT TCGATCCAAG CAAAAAAGAG GTTCTTGGTA GGACAATACG GTAAGAAAAC ACGACATGTG CAGAGATGCC GACGAAGCAT AGTTAAACTG GGATGGTAAA ATCAATTAAG AATTTATAAA GACAAAATTG TATAAGTCTC TAAAACATCT TAATTATACC TCACAGAACT ATCTAAAATA TATTCACAAA GTGCAAACAT TATCATGAAA AAGAACCATT TTAATTTAAA GCAAGGGCAT TAAGGCTTAT TTACAGAATT TCTTACTTTT GTAAAGATTA TAAGGCTGAT TATCTTTTTC ACCATGCCAA AAATTACACA AGATAGAATG GATGTTTGAA ATTAAACGTG AGTATACAAA CAAATACACT AGGTAAATCG AAACATTTTT TTCTCCATTA AGTAACAAAT TCCTATTTAG AGAAAGAATG CTGAGTAGAT TAAATAATCT ATACAAACTT TTTTAACACA AATGCATACA TATAGCCAGT GGGATTTGTA GCTAAGCTTC CGAATTCGAG TCTAACTCCT TAACCACTCG GACATAGTGA CTTATCTGAC ACTCATTGTA AATTAATATA TATATAGGCA TTTTGTTTAG TTAAAGGTAC TTAAGTAATT AGTATAAACG AACCAATTTT ATAATCAGGA AGTTAAGTGA ATGGTAGCAC ATGTCGTAAA AATTGTGAAT TTTTATTGAA TAATATTTTA AATACAAGCC TTTCTAGACT AGGTATACTC ATAAACATAT ATGAGCAAAA GGATAGAGGA GATTACATTG CATCTTCTAC AAATTTATTT ATTGCCCTTT ACTGAAAAAT TAAATAATGA GTACTAAATG ATAAAAAGCG CTCAGTACAA GAAAGATTGC AAAAATATTG CATTCTTCAT GAATTAAAGT TGCATATAAG GCATATTGAA AGTAATAGTA CTAAAACAGC AGTTAGCGAA AATTAATAGA ATTATATTCG CAAGACAATT GTGACATTAA ATTAAAAAAT TGTAGAATTT TTACTATCCT CTTTAACGCC ATGAGCCTTT ATAAAAAGGT TAAATTAGTT TTAACATTCT TTTTTTGAGT AAGAGTTTAC AATTTATCAA AACCTGTGTT ATTATATTCA TTAGTTTCAA TTTATTAGCA TCTAGAGAAA AATCAATTGG CAGTTACATT GTTGGAATTT ATGAAGAAAA AGAATCTACA ACGGAGAATA AGTTGCTGAT CGCTTTCCCT AAAATTGTAT ATTTTGCTGA GCTTATTTTG ACATTTCGTT GAAGTTTTCT CTAATTCGCA TTCATTTTAA GTAAAACAAT GAGAAATAAA ATTACAAAAA ATACAAATTA AAATACAATT TTTAGCTATA ATTATAGACG ATGCCCTTGT ATCCCATTCT GTCTCGCTTG CCCCTACTTT TTATCTTTTA TATACCATAA TGAACGCTGC CGCTACTAAC CATACCCCGA TTTTACATTT CGGACTCCCA AGGACGTACA AAATAGAAAA CTATAGAAAA AAATAATCAG AAAATAGCAT GTCATCTCTT TGTAAAACGC GTTTGCAAGA AGAAAGGAAA CAATGGAGAA GAGATCATCC ATTTGTATGT AAATTTTAGT AAACTTGAAG AAATCACTAA CAACTTCTCT TACTTAGGGA TTCTATGCAA AACCTTGTAA ATCATCTGAT GGAGGACTCG ATTTAATGAA TTGGAAGGTT GGGCCCGCGG CCGCTTAGCT GAAACTTCCA ACCCTCCTGC TCCCGTCACA TTACTCGACA AAGCTAATGT TTTGGCAACT TCTCGTCTTT GGCGCAAGAC TGTAGCTAAG ATTTTTAAGG AGGAATATCC TCACCTTACC TTAAAAAACC AGCTCATTGA CTCCGCAGCC ATGCTTTTGG TCAAGAGCCC TCGTACACTT AACGGTGTTG TTTTGACTGA CAACTTGTTT GGTGACATTA TTTCAGATGA GGCTTCTGTC ATTCCTGGTA GCTTGGGCCT TTTGCCTTCT GCCTCCCTTT CCGGTGTGGT AGGAAAATCA GAAGAAAAGG TTCATTGTTT GGTTGAGCCC ATTCACGGTA GCGCTCCCGA GCTCGAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGCATGCCTG CAGGTCGATC GACTCTAGAG GATCAGAAAA TTATCGCCAT AAAAGACAGA ATAAGTCATC AGCGGTTGTT TCATTTCCTA TATTTTTTTT TTATTTTTTT ATTTTTTAAT AAGGGAAAAT TTAACGTCTA AGGATACAGA AGATTGTTAG CACATTAAAG TAATAAAGGC TTAAGTAGTA AGTGCCTTAG CATGTTATTG TATTTCAAAG GACATAATCT AAAATAATAA CAATATCATT TCTCACAAGT TATTCAATTT TCTTTTTTTT TTCTAATAAT ATCAAGAATG TATTATTTGT TTGACATAAG TCAACTAATT TATTTAATAT GCTGGATTAA TCTTGCAGAC ATGTAAATTA ACAAGTTTTA GTCAAATAAC GTTGAAGTTT CAATGAACTC AAATAATTTC TCTTTTTTTT TATATAACTC TAATCTGATT TATATTTTCC GCAGGGATCA ACTGAAGTTA TGACATTTGG ATTGGATCAC TTATAACCTT GGTCGCCAAA TAATACAAAA ATCAGCGTTA TAAAACAAAG AAGGTTTTTG TTAAGAAATT AATCCTCTTT CTTGATAAGA AAGTTGAACC GAAATTGCAG ATACTGATAT ATGAAAATAA TACCCACAAT TTTGGGAATA GCGCAAGCCT CAATTTAAAC AATAGGTGAG GACACATGAT AATGACCTCA ATGATTGTTA GAAGAAAAGA GCCTCATTAC AAAATCGAAA AATGAATGGT TGGGTACAAG TTTCCAAAAC ATGGTAAAGT GGACTTTGCG TATGAGACGT AAATAGAAAA AAACACTTGT TATATGTTTT CTAGAATTAT TGTTGTCTCT TTATGGTTGG ATGATGCAAA ATAGTAATTT CGGTTAGTTG CTGTAAAACA CCACGAGACA AATAGATATG GATATTTATT AAATCAGGAA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCTATGCAT CACCACCATC ATCATATGGA TTATAAGGAT GATGACGATA AACCCATGGG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCGTGGCCAA CACTTGTCAC TACTTTCTGT TATGGTGTTC AATGCTTTTC AAGATACCCA GATCACATGA AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGCTA TGTACAGGAA AGAACTATAT TTTTCAAAGA TGACGGGAAC TACAAGACAC GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAG ATATCATGAG ATCTCGACTC TAGAGGATCC
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