pMIND-RC1973
$128.00
In stock
SKU
PL80438
H37Rv
100μg liquid plasmid(Instantly):$149
| name | pMIND-RC1973 | another name | apMIND-RC1973 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | TetRO | Replicon | OriM |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGGATAACA ATTTCACACA GGAAACAGCT ATGACATGAT TACGAATTAG CCCGCCTAAT GAGCGGGCTT TTTTTTGAAT TAGCCCGCCT AATGAGCGGG CTTTTTTTTG AATTCGAGCT CGGTACCTCT AGATCACGAT TCGCTCGAGG TCAATGACGC TGTGCGGGAG TGAGAGAATC CGTCGATCAG TGCTGTCAGG CCGAAATCGA ATTCCCGGTC GCGTTCAGGA ACGCTCGCAT TGCCGACCTC TGCGGTTTGC TCCTCCAGTA CGACACCGAC GGTGTATCGG CTGACCGCGA TGAGCATGCT GATGGCTGTC TGTTCATCGA ATCCCTGAGA CACGAGAAGC CCGACTTGCT GACCGGAAGC CTCAGCTCCT TCGTCGGTGT CGTCGCCGGA TGGTCTGCGG TGGTGCTCAG CGTGGAGACG CGCACCGTCA CGGACCGCGT GTAGTGCGTG GCGGAAACTT CTTGCGTTTC GCAAGAGAAA TGCGTCCCAT TTCTCGTCGG ACTCGGGGAA GGAAGCGTGA TGCTCTCGGT CAAGCACGTC GCTCGCCAGC GCTGCGAGGA GTTCGGCCTT CGTGCGGAAG TGCCAGTAGA GGCCGGGCTG CTGTACCTGT AAGTGAGCCG CCAGCGCGCG AGTGGTGAAG CCATCGAGCC CAGTCTCGTC GAGCACCTGC CGGGCCCCGA GCAACACGGA CGTGCGGTCG AGACGCTTCC GGTGGTGAGT CATAGTTGCA CTTTATCATC GATAACTCTA TCTTAGATAA AGTGACTGCT CGCTACTCTC ATCTGACTGC TCGCTACTCT CATCGTGGAA TCCTGACAGG ATCCCTTAAG AACTGCAGTC CATATGGATA TCATGAGCTG GTCGCGGGTG ATCGCCTACG GGCTGCTGCC CGGGCTGGCG TTGGCGCTGA CGTGTGGCGC GGGCTTGCTG AAATGGCAGG ACGGCGCCGT CCGCGACGCC GCGGTTGCCC GTGCGGAATC CGTGCGGGCC GCGACCGACG GCACCACCGC GCTGCTGTCT TACCGGCCCG ACACCGTGCA GCATGACCTC GAGAGCGCGC GAAGCAGGCT CACGGGCACG TTCCTCGACG CCTACACACA GCTGACCCAC GACGTGGTGA TCCCCGGCGC ACAGCAGAAG CAGATCTCGG CCGTGGCCAC CGTCGCGGCC GCGGCGTCGG TGTCGACTTC CGCCGACCGC GCCGTCGTCC TGCTGTTCGT AAACCAGACC ATCACCGTCG GCAAGGACGC GCCGACCACC GCCGCTTCCA GCGTTCGGGT GACCCTCGAC AACATCAACG GGCGTTGGCT GATCTCGCAA TTCGAACCGA TCACTAGTGC ATGCGTGACA CAAGAATCCC TGTTACTTCT CGACCGTATT GATTCGGATG ATTCCTACGC GAGCCTGCGG AACGACCAGG AATTCTGGGA GCCGCTGGCC CGCCGAGCCC TGGAGGAGCT CGGGCTGCCG GTGCCGCCGG TGCTGCGGGT GCCCGGCGAG AGCACCAACC CCGTACTGGT CGGCGAGCCC GGCCCGGTGA TCAAGCTGTT CGGCGAGCAC TGGTGCGGTC CGGAGAGCCT CGCGTCGGAG TCGGAGGCGT ACGCGGTCCT GGCGGACGCC CCGGTGCCGG TGCCCCGCCT CCTCGGCCGC GGCGAGCTGC GGCCCGGCAC CGGAGCCTGG CCGTGGCCCT ACCTGGTGAT GAGCCGGATG ACCGGCACCA CCTGGCGGTC CGCGATGGAC GGCACGACCG ACCGGAACGC GCTGCTCGCC CTGGCCCGCG AACTCGGCCG GGTGCTCGGC CGGCTGCACA GGGTGCCGCT GACCGGGAAC ACCGTGCTCA CCCCCCATTC CGAGGTCTTC CCGGAACTGC TGCGGGAACG CCGCGCGGCG ACCGTCGAGG ACCACCGCGG GTGGGGCTAC CTCTCGCCCC GGCTGCTGGA CCGCCTGGAG GACTGGCTGC CGGACGTGGA CACGCTGCTG GCCGGCCGCG AACCCCGGTT CGTCCACGGC GACCTGCACG GGACCAACAT CTTCGTGGAC CTGGCCGCGA CCGAGGTCAC CGGGATCGTC GACTTCACCG ACGTCTATGC GGGAGACTCC CGCTACAGCC TGGTGCAACT GCATCTCAAC GCCTTCCGGG GCGACCGCGA GATCCTGGCC GCGCTGCTCG ACGGGGCGCA GTGGAAGCGG ACCGAGGACT TCGCCCGCGA ACTGCTCGCC TTCACCTTCC TGCACGACTT CGAGGTGTTC GAGGAGACCC CGCTGGATCT CTCCGGCTTC ACCGATCCGG AGGAACTGGC GCAGTTCCTC TGGGGGCCGC CAGACACCGC CCCCGGCGCC TAAAAGCTTG GCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GATAAGCTAG CCCAACCAGC CCGCGAACGG CTGGCAGCCA GCGTAAGGCG CGGCTCATCG GGCGGCGTTC GCCACGATGT CCTGCACTTC GAGCCAAGCC TCGAACACCT GCTGGTGTGC ACGACTCACC CGGTTGTTGA CACCGCGCGC GGCCGTGCGG GCTCGGTGGG GCGGCTCTGT CGCCCTTGCC AGCGTGAGTA GCGCGTACCT CACCTCGCCC AACAGGTCGC ACACAGCCGA TTCGTACGCC ATAAAGCCAG GTGAGCCCAC CAGCTCCGTA AGTTCGGGCG CTGTGTGGCT CGTACCCGCG CATTCAGGCG GCAGGGGGTC TAACGGGTCT AAGGCGGCGT GTACGGCCGC CACAGCGGCT CTCAGCGGCC CGGAAACGTC CTTGAAACGA CGTATGTGTT CCTCCTGGTT GGTACAGGTG GTTGGGGGTG CTCGGCTGTC GCTGGTGTTC CACCACCAGG GCTCGACGGG AGAGCGGGGG AGTGTGCAGT TGTGGGGTGG CCCCTCAGCG AAATATCTGA CTTGGAGCTC GTGTCGGACC ATACACCGGT GATTAATCGT GGTCTACTAC CAAGCGTGAG CCACGTCGCC GACGAATTTG AGCAGCTCTG GCTGCCGTAC TGGCCGCTGG CAAGCGACGA TCTGCTCGAG GGGATCTACC GCCAAAGCCG CGCGTCGGCC CTAGGCCGCC GGTACATCGA GGCGAACCCA ACAGCGCTGG CAAACCTGCT GGTCGTGGAC GTAGACCATC CAGACGCAGC GCTCCGAGCG CTCAGCGCCC GGGGGTCCCA TCCGCTGCCC AACGCGATCG TGGGCAATCG CGCCAACGGC CACGCACACG CAGTGTGGGC ACTCAACGCC CCTGTTCCAC GCACCGAATA CGCGCGGCGT AAGCCGCTCG CATACATGGC GGCGTGCGCC GAAGGCCTTC GGCGCGCCGT CGACGGCGAC CGCAGTTACT CAGGCCTCAT GACCAAAAAC CCCGGCCACA TCGCCTGGGA AACGGAATGG CTCCACTCAG ATCTCTACAC ACTCAGCCAC ATCGAGGCCG AGCTCGGCGC GAACATGCCA CCGCCGCGCT GGCGCCAGCA GACCACGTAC AAAGCGGCTC CGACGCCGCT AGGGCGGAAT TGCGCACTGT TCGATTCCGT CAGGTTGTGG GCCTATCGTC CCGCCCTCAT GCGGATCTAC CTGCCGACCC GGAACGTGGA CGGACTCGGC CGCGCGATCT ATGCCGAGTG CCACGTCCGA AACGCCGAGT TCCCGTGCAA CGACGTGTGT CCCGGACCGC TACCGGACAG CGAGGTCCGC GCCATCGCCA ACAGCATTTG GCGTTGGATC ACAACCAAGT CGCGCATTTG GGCGGACGGG ATCGTGGTCT ACGAGGCCAC ACTCAGTGCG CGCCAGTCGG CCATCTCGCG GAAGGGCGCA GCAGCGCGCA CGGCGGCGAG CACAGTTGCG CGGCGCGCAA AGTCCGCGTC AGCCATGGAG GCATTGCTAT GAGCGACGGC TACAGCGACG GCTACAACCG GCAGCCGACT GTCCGCAAAA AGCGGCGCGT GACCGCCGCC GAAGGCGCTC GAATCACCGG ACTATCCGAA CGCCACGTCG TCCGGCTCGT GGCGCAGGAA CGCAGCGAGT GGCTCGCCGA GCAGGCTGCA CGCCGCGAAC GCATCCGCGC CTATCACGAC GACGAGGGCC ACTCTTGGCC GCAAACGGCC AAACATTTCG GGCTGCATCT GGACACCGTT AAGCGACTCG GCTATCGGGC GAGGAAAGAG CGTGCGGCAG AACAGGAAGC GGCTCAAAAG GCCCACAACG AAGCCGACAA TCCACCGCTG TTCTAACGCA ATTGGGGAGC GGGTGTCGCG GGGGTTCCGT GGGGGGTTCC GTTGCAACGG GTCGGACAGG TAAAAGTCCT GGTAGACGCT AGTTTTCTGG TTTGGGCCAT GCCTGTCTCG TTGCGTGTTT CGTTGCGCCC GTTTTGAATA CCAGCCAGAC GAGACGGGGT TCTACGAATC TTGGTCGATA CCAAGCCATT TCCGCTGAAT ATCGGGGAGC TCACCGCCAG AATCGGTGGT TGTGGTGATG TACGTGGCGA ACTCCGTTGT AGTGCCTGTG GTGGCATCCG TGGCCACTCT CGTTGCACGG TTCGTTGTGC CGTTACAGGC CCCGTTGACA GCTCACCGAA CGTAGTTAAA ACATGCTGGT CAAACTAGGT TTACCAACGA TACGAGTCAG CTCATCTAGG GCCAGTTCTA GGCGTTGTTC GTTGCGCGGT TCGTTGCGCA TGTTTCGTGT GGTTGCTAGC TTCACGCTGC CGCAAGCACT CAGGGCGCAA GGGCTGCTAA AGGAAGCGGA ACACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC AGCTACTGGG CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC AGTGGGCTTA CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA TTGCCAGCTG GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTTGCCGC CAAGGATCTG ATGGCGCAGG GATCAAGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT CCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GGATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGA
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