PDHB1
$99.00
In stock
SKU
PL80425
100μg liquid plasmid(Instantly):$120
| name | PDHB1 | another name | aPDHB1 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCGCCGGGAT CGAAGAAATG ATGGTAAATG AAATAGGAAA TCAAGGAGCA TGAAGGCAAA AGACAAATAT AAGGGTCGAA CGAAAAATAA AGTGAAAAGT GTTGATATGA TGTATTTGGC TTTGCGGCGC CGAAAAAACG AGTTTACGCA ATTGCACAAT CATGCTGACT CTGTGGCGGA CCCGCGCTCT TGCCGGCCCG GCGATAACGC TGGGCGTGAG GCTGTGCCCG GCGGAGTTTT TTGCGCCTGC ATTTTCCAAG GTTTACCCTG CGCTAAGGGG CGAGATTGGA GAAGCAATAA GAATGCCGGT TGGGGTTGCG ATGATGACGA CCACGACAAC TGGTGTCATT ATTTAAGTTG CCGAAAGAAC CTGAGTGCAT TTGCAACATG AGTATACTAG AAGAATGAGC CAAGACTTGC GAGACGCGAG TTTGCCGGTG GTGCGAACAA TAGAGCGACC ATGACCTTGA AGGTGAGACG CGCATAACCG CTAGAGTACT TTGAAGAGGA AACAGCAATA GGGTTGCTAC CAGTATAAAT AGACAGGTAC ATACAACACT GGAAATGGTT GTCTGTTTGA GTACGCTTTC AATTCATTTG GGTGTGCACT TTATTATGTT ACAATATGGA AGGGAACTTT ACACTTCTCC TATGCACATA TATTAATTAA AGTCCAATGC TAGTAGAGAA GGGGGGTAAC ACCCCTCCGC GCTCTTTTCC GATTTTTTTC TAAACCGTGG AATATTTCGG ATATCCTTTT GTTGTTTCCG GGTGTACAAT ATGGACTTCC TCTTTTCTGG CAACCAAACC CATACATCGG GATTCCTATA ATACCTTCGT TGGTCTCCCT AACATGTAGG TGGCGGAGGG GAGATATACA ATAGAACAGA TACCAGACAA GACATAATGG GCTAAACAAG ACTACACCAA TTACACTGCC TCATTGATGG TGGTACATAA CGAACTAATA CTGTAGCCCT AGACTTGATA GCCATCATCA TATCGAAGTT TCACTACCCT TTTTCCATTT GCCATCTATT GAAGTAATAA TAGGCGCATG CAACTTCTTT TCTTTTTTTT TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT TCCAGTTACT TGAATTTGAA ATAAAAAAAA GTTTGCTGTC TTGCTATCAA GTATAAATAG ACCTGCAATT ATTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT CTTCCTTGTT TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCCA AGCTGGCCGC TCTAGAGCAA GCAAAAGATG ATCTCTGATG AACAGCTGAA CTCCTTGGCC ATCACCTTCG GTATTGTGAT GATGACTTTA ATTGTCATTT ACCATGCTGT TGACTCCACC ATGTCTCCTA AGAACGCGGC CATTACGGCC AGGCCTCCAT GGTATATCTT GGCCGCCTCG GCCATGTCGG GGGGGATCCC TCCAGATCAA CAAAGATTGA TCTTTGCCGG TAAGCAGCTA GAAGACGGTA GAACGCTGTC TGATTACAAC ATTCAGAAGG AGTCCACCTT ACATCTTGTG CTAAGGCTAA GAGGTGGTAT GCACAGATCA GCTTGCGGCC GCATGAAAGC GTTAACGGCC AGGCAACAAG AGGTGTTTGA TCTCATCCGT GATCACATCA GCCAGACAGG TATGCCGCCG ACGCGTGCGG AAATCGCGCA GCGTTTGGGG TTCCGTTCCC CAAACGCGGC TGAAGAACAT CTGAAGGCGC TGGCACGCAA AGGCGTTATT GAAATTGTTT CCGGCGCATC ACGCGGGATC CGTCTGTTGC AGGAAGAGGA AGAAGGGTTG CCGCTGGTAG GTCGTGTGGC TGCCGGTGAA CCACTTCTGG CGCAACAGCA TATTGAAGGT CATTATCAGG TCGACCCTTC CTTATTCAAG CCGAATGCTG ATTTCCTGCT GCGCGTCAGC GGGATGTCGA TGAAAGATAT CGGCATTATG GATGGTGACT TGCTGGCAGT GCATAAAACT CAGGATGTAC GTAACGGTCA GGTCGTTGTC GCACGTATTG ATGACGAAGT TACCGTTAAG GGCCTGAAAA AACAGGGCAA TAAAGTCGAA CTGTTGCCAG AAAATAGCGA GTTTAAACCA ATTGTCGTAG ATCTTCGTCA GCAGAGCTTC ACCATTGAAG GGCTGGCGGT TGGGGTTATT CGCAACGGCG ACTGGCTGGA ATTCCCGGGG ATCTGGGCCC CCCCGACCGA TGTCAGCCTG GGGGACGAGC TCCACTTAGA CGGCGAGGAC GTGGCGATGG CGCATGCCGA CGCGCTAGAC GATTTCGATC TGGACATGTT GGGGGACGGG GATTCCCCGG GTCCGGGATT TACCCCCCAC GACTCCGCCC CCTACGGCGC TCTGGATATG GCCGACTTCG AGTTTGAGCA GATGTTTACC GATGCCCTTG GAATTGACGA GTACGGTGGG TAGGTCATGT AATTAGTTAT GTCACGCTTA CATACACGCC CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GTTTAATTTG GGGGGCCCGG TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT CGACGGTCGA GGAGAACTTC TAGTATATCC ACATACCTAA TATTATTGCC TTATTAAAAA TGGAATCCCA ACAATTACAT CAAAATCCAC ATTCTCTTCA AAATCAATTG TCCTGTACTT CCTTGTTCAT GTGTGTTCAA AAACGTTATA TTTATAGGAT AATTATACTC TATTTCTCAA CAAGTAATTG GTTGTTTGGC CGAGCGGTCT AAGGCGCCTG ATTCAAGAAA TATCTTGACC GCAGTTAACT GTGGGAATAC TCAGGTATCG TAAGATGCAA GAGTTCGAAT CTCTTAGCAA CCATTATTTT TTTCCTCAAC ATAACGAGAA CACACAGGGG CGCTATCGCA CAGAATCAAA TTCGATGACT GGAAATTTTT TGTTAATTTC AGAGGTCGCC TGACGCATAT ACCTTTTTCA ACTGAAAAAT TGGGAGAAAA AGGAAAGGTG AGAGGCCGGA ACCGGCTTTT CATATAGAAT AGAGAAGCGT TCATGACTAA ATGCTTGCAT CACAATACTT GAAGTTGACA ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT AGCAATCGTC TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA TTTCAAGGAT ATACCATTCT AATGTCTGCC CCTATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT CCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGTT GATGCCGTTT TGTTAGGTGC TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AATTCCCTAC ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA AGAACCCAAC CCACCTAAAT GGTATTATAA TCACCAGCAA CATGTTTGGT GATATCATCT CCGATGAAGC CTCCGTTATC CCAGGTTCCT TGGGTTTGTT GCCATCTGCG TCCTTGGCCT CTTTGCCAGA CAAGAACACC GCATTTGGTT TGTACGAACC ATGCCACGGT TCTGCTCCAG ATTTGCCAAA GAATAAGGTT GACCCTATCG CCACTATCTT GTCTGCTGCA ATGATGTTGA AATTGTCATT GAACTTGCCT GAAGAAGGTA AGGCCATTGA AGATGCAGTT AAAAAGGTTT TGGATGCAGG TATCAGAACT GGTGATTTAG GTGGTTCCAA CAGTACCACC GAAGTCGGTG ATGCTGTCGC CGAAGAAGTT AAGAAAATCC TTGCTTAAAA AGATTCTCTT TTTTTATGAT ATTTGTACAT AAACTTTATA AATGAAATTC ATAATAGAAA CGACACGAAA TTACAAAATG GAATATGTTC ATAGGGTAGA CGAAACTATA TACGCAATCT ACATACATTT ATCAAGAAGG AGAAAAAGGA GGATAGTAAA GGAATACAGG TAAGCAAATT GATACTAATG GCTCAACGTG ATAAGGAAAA AGAATTGCAC TTTAACATTA ATATTGACAA GGAGGAGGGC ACCACACAAA AAGTTAGGTG TAACAGAAAA TCATGAAACT ACGATTCCTA ATTTGATATT GGAGGATTTT CTCTAAAAAA AAAAAAATAC AACAAATAAA AAACACTCAA TGACCTGACC ATTTGATGGA GTTTAAGTCA ATACCTTCTT GAACCATTTC CCATAATGGT GAAAGTTCCC TCAAGAATTT TACTCTGTCA GAAACGGCCT TACGACGTAG TCGATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGACCACA TGTGGATGAT CCAGTTCGAT TTATTCAACA AAGCCGCCGT CCCGTCAAGT CAGCGTAATG CTCTGCCAGT GTTACAACCA ATTAACCAAT TCTGATTAGA AAAACTCATC GAGCATCAAA TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGC TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCATCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCTGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCGGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCCTCG AGCAAGACGT TTCCCGTTGA ATATGGCTCA TAACACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTGGCTT ACTAGGATCG ATCCGACATG TGGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCTCG CTTGCCTGTA ACTTACACGC GCCTCGTATC TTTTAATGAT GGAATAATTT GGGAATTTAC TCTGTGTTTA TTTATTTTTA TGTTTTGTAT TTGGATTTTA GAAAGTAAAT AAAGAAGGTA GAAGAGTTAC GGAATGAAGA AAAAAAAATA AACAAAGGTT TAAAAAATTT CAACAAAAAG CGTACTTTAC ATATATATTT ATTAGACAAG AAAAGCAGAT TAAATAGATA TACATTCGAT TAACGATAAG TAAAATGTAA AATCACAGGA TTTTCGTGTG TGGTCTTCTA CACAGACAAG ATGAAACAAT TCGGCATTAA TACCTGAGAG CAGGAAGAGC AAGATAAAAG GTAGTATTTG TTGGCGATCC CCCTAGAGTC TTTTACATCT TCGGAAAACA AAAACTATTT TTTCTTTAAT TTCTTTTTTT ACTTTCTATT TTTAATTTAT ATATTTATAT TAAAAAATTT AAATTATAAT TATTTTTATA GCACGTGATG AAAAGGACCC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTACCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CC
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