pMD-AOX-Laccase
$113.00
In stock
SKU
PL80312
自主复制
100μg liquid plasmid(Instantly):$134
| name | pMD-AOX-Laccase | another name | apMD-AOX-Laccase |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAACATAT GAGATTTCCT TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA AGAGAGGCTG AAGCTTACGT AGAATTCGCT ATTGGCCCTG TAGCTGATCT CCACATTTCC AATGCTAACA TCTCTCCCGA TGGCTTCACT CGTGCAGCCG TTCTCGCTGG CGGGTCCTTC CCTGGACCTC TTATTACTGG CAATAAGGGT GACAACTTCC AGATCAACGT GATCAACGAC CTCACCGATG CAGACCAACT CAAGACCACC ACCATTCACT GGCATGGTTT CTTCCAGCAC GGTACTAACT GGGCTGACGG CCCGGCTTTC ATCAATCAGT GTCCTATTGC CTCCGGCAAC TCGTTCTTGT ACAACTTCCA AGTTCCTGAC CAAGCTGGTA CATTCTGGTA TCACAGCCAT CTCTCGACTC AGTATTGTGA CGGACTCCGC GGAGCATTCG TGGTCTATGA TCCCGATGAC CCACATGCAA GTTTGTACGA CGTCGATGAT GAGAGTACCG TCATCACTCT TGCAGACTGG TACCATGACC TAGCGCGGTT GGGTCCGAAG TTCCCCACCA CTAACTCGAC ACTCATTAAC GGCCTTGGTC GTTACGATTT CGGACCCTCC TCCGACTTGG CTGTCATCTC CGTGCAAGCT GGAAAGCGGT ACCGTTTCCG TCTGGTCTCT ATCTCGTGTG ACTCGAACTA CGTGTTCTCC ATCGACGAGC ACACATTCAC CGTCATCGAA GTCGACGGTG TCAACCACCA ACCTGTCGAG GCTGATTCCC TCCAAATCTT CCCTGGTCAG CGGTACTCCA TCGTGGTCAA TGCCGACAAA TCGGAAGGAG GTAACTATTG GATTCGCGCC TCACCTACTC CTGGTCCCCA AGGATTTGCC AATGGAATCA ACTCTGCTAT CTTGCGCTAT GTCGGTTCCG ATGAGGTTGA GCCTACTACG AACCAGACGA CGAGCACAAA CCCGCTTGTG GAGGCTAACC TGGTTCCCCT TGAGAACCCT GGAGCGCCTG GCGATCCCAC ACCCGGCGGT GTAGATGTCC CCCTCAACCT TGCAGTCGCT TTCGATGGTA CAGGACTCGA CTTCCAGGTC AACGGACAGA CGTTCATCCC CCCTACCGTT CCCGTCTTGC TGCAAATTCT CAGCGGTGCT CAATCTGCCA CAGACCTCCT CCCCAGCGGA AGCGTCTACG TTCTTCCGAG CAACGCTACG GTCGAAATAA GCATTCCAGC TGGCGCCGTC GGCGGTCCTC ACCCCATTCA TTTGCACGGT CACACATTCG ATGTCGTCCG AAGTGCCGGC AGCAGCACTT ATAACTATGT CAACCCGCCT CGTCGCGACG TCGTCAGCAT TGGTAACGCC GGTGACAACG TAACCATTCG TTTCCGGACT GATAACCCTG GACCATGGTT CCTCCATTGC CACATCGACT GGCATCTCGA AGCCGGCTTT GCTGTTGTCT TCGCTGAAGA CATCCCCAAC GTCGCCTCAG TCAACTCCCC TCCTCAGGCC TGGAGCGACC TTTGCCCTAT CTATGATGCG CTTGACCCTT CTGACCACTG AGCGGCCGCT CTAGAACAAA AACTCATCTC AGAAGAGGAT CTGAATAGCG CCGTCGACCA TCATCATCAT CATCATTGAG TTTGTAGCCT TAGACATGAC TGTTCCTCAG TTCAAGTTGG GCACTTACGA GAAGACCGGT CTTGCTAGAT TCTAATCAAG AGGATGTCAG AATGTCATTT GCCTGAGAGA TGCAGGCTTC ATTTTTGATA CTTTTTTATT TGTAACCTAT ATAGTATAGG ATTTTTTTTG TCATTTTGTT TCTTCTCGTA CGAGCTTGCT CCTGATCAGC CTATCTCGCA GCTGATGAAT ATCTTGTGGT AGGGGTTTGG GAAAATCATT CGAGTTTGAT GTTTTTCTTG GTATTTCCCA CTCCTCTTCA GAATACAGAA GATTAAGTGA GACCTTCGTT TGTGCGGATC CGCTAGCTTA GTGTACTACC CAATCTTGCC TCTTCGGGTG TCTTTTATAA GGACAGATTC ACAAGCTCTT GTTGCCCAAT ACACACATAC ACACAGAGAT AATAGCAGTC TTAATTAAAT GAAAATGAGC CATATTCAAC GGGAAACGTC TTGCTCAAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCT AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA AGATCTCACG TCCGACGGGC GGCCCACGGG TCCCAGGCCT CGGAGATCCG TCCCCCTTTT CCTTTGTCGA TATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT CCGCTCTAAC CAAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC TTTAATTTGC AAGCTAGTAC TTTTAATCGT CCACTTGCCG TCATGCTTCT TTGGCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGAACTCGTC CTCGGTTCCC GGCGATCCTC TGGAGATTGC TTACCAAAAA TGGCCCACTG AGGGGGTCTA GTATTTGAAG AAGCCTCACT AAAGCAGGTC TTCGCCACAC ATTTAAAGTT TGAGAATGGG CGGAGGTCAA CCCCCCGTCC CCCTAATCAT TGCTTTACCT CATGGAACTA AACAACCACC CCCCCGCTAT CCTGAGGGAA ACTTCGGCAG GAACCAGCTA CTAGATGGTT CGATTAGTCT TTCGCCCCTA TACCCAAATT CGACGATCGA TTTGCACGTC AGAACCGCTG CGGGCCTCCA CCAGAGTTTC CTCAGGCTTC GCCCTATTCA GGCATAGTTC ACCATCTACT AGT
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