pKD20
$99.00
In stock
SKU
PL79628
100μg liquid plasmid(Instantly):$134
| name | pKD20 | another name | apKD20 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | araBAD | Replicon | pSC101 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | rrnBT1-F：GTTGTTTGTCGGTGAACGCTC | |
| 3′ sequencing primer | PKD-R：CAGTGACACAGGAACACTT | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATCGATTTA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT ACCCGTTTTT TTGGGAATTC GAGCTCTAAG GAGGTTATAA AAAATGGATA TTAATACTGA AACTGAGATC AAGCAAAAGC ATTCACTAAC CCCCTTTCCT GTTTTCCTAA TCAGCCCGGC ATTTCGCGGG CGATATTTTC ACAGCTATTT CAGGAGTTCA GCCATGAACG CTTATTACAT TCAGGATCGT CTTGAGGCTC AGAGCTGGGC GCGTCACTAC CAGCAGCTCG CCCGTGAAGA GAAAGAGGCA GAACTGGCAG ACGACATGGA AAAAGGCCTG CCCCAGCACC TGTTTGAATC GCTATGCATC GATCATTTGC AACGCCACGG GGCCAGCAAA AAATCCATTA CCCGTGCGTT TGATGACGAT GTTGAGTTTC AGGAGCGCAT GGCAGAACAC ATCCGGTACA TGGTTGAAAC CATTGCTCAC CACCAGGTTG ATATTGATTC AGAGGTATAA AACGAATGAG TACTGCACTC GCAACGCTGG CTGGGAAGCT GGCTGAACGT GTCGGCATGG ATTCTGTCGA CCCACAGGAA CTGATCACCA CTCTTCGCCA GACGGCATTT AAAGGTGATG CCAGCGATGC GCAGTTCATC GCATTACTGA TCGTTGCCAA CCAGTACGGC CTTAATCCGT GGACGAAAGA AATTTACGCC TTTCCTGATA AGCAGAATGG CATCGTTCCG GTGGTGGGCG TTGATGGCTG GTCCCGCATC ATCAATGAAA ACCAGCAGTT TGATGGCATG GACTTTGAGC AGGACAATGA ATCCTGTACA TGCCGGATTT ACCGCAAGGA CCGTAATCAT CCGATCTGCG TTACCGAATG GATGGATGAA TGCCGCCGCG AACCATTCAA AACTCGCGAA GGCAGAGAAA TCACGGGGCC GTGGCAGTCG CATCCCAAAC GGATGTTACG TCATAAAGCC ATGATTCAGT GTGCCCGTCT GGCCTTCGGA TTTGCTGGTA TCTATGACAA GGATGAAGCC GAGCGCATTG TCGAAAATAC TGCATACACT GCAGAACGTC AGCCGGAACG CGACATCACT CCGGTTAACG ATGAAACCAT GCAGGAGATT AACACTCTGC TGATCGCCCT GGATAAAACA TGGGATGACG ACTTATTGCC GCTCTGTTCC CAGATATTTC GCCGCGACAT TCGTGCATCG TCAGAACTGA CACAGGCCGA AGCAGTAAAA GCTCTTGGAT TCCTGAAACA GAAAGCCGCA GAGCAGAAGG TGGCAGCATG ACACCGGACA TTATCCTGCA GCGTACCGGG ATCGATGTGA GAGCTGTCGA ACAGGGGGAT GATGCGTGGC ACAAATTACG GCTCGGCGTC ATCACCGCTT CAGAAGTTCA CAACGTGATA GCAAAACCCC GCTCCGGAAA GAAGTGGCCT GACATGAAAA TGTCCTACTT CCACACCCTG CTTGCTGAGG TTTGCACCGG TGTGGCTCCG GAAGTTAACG CTAAAGCACT GGCCTGGGGA AAACAGTACG AGAACGACGC CAGAACCCTG TTTGAATTCA CTTCCGGCGT GAATGTTACT GAATCCCCGA TCATCTATCG CGACGAAAGT ATGCGTACCG CCTGCTCTCC CGATGGTTTA TGCAGTGACG GCAACGGCCT TGAACTGAAA TGCCCGTTTA CCTCCCGGGA TTTCATGAAG TTCCGGCTCG GTGGTTTCGA GGCCATAAAG TCAGCTTACA TGGCCCAGGT GCAGTACAGC ATGTGGGTGA CGCGAAAAAA TGCCTGGTAC TTTGCCAACT ATGACCCGCG TATGAAGCGT GAAGGCCTGC ATTATGTCGT GATTGAGCGG GATGAAAAGT ACATGGCGAG TTTTGACGAG ATCGTGCCGG AGTTCATCGA AAAAATGGAC GAGGCACTGG CTGAAATTGG TTTTGTATTT GGGGAGCAAT GGCGATGATA AGCTAGCCCA TGGGTATGGA CAGTTTTCCC TTTGATATGT AACGGTGAAC AGTTGTTCTA CTTTTGTTTG TTAGTCTTGA TGCTTCACTG ATAGATACAA GAGCCATAAG AACCTCAGAT CCTTCCGTAT TTAGCCAGTA TGTTCTCTAG TGTGGTTCGT TGTTTTTGCG TGAGCCATGA GAACGAACCA TTGAGATCAT ACTTACTTTG CATGTCACTC AAAAATTTTG CCTCAAAACT GGTGAGCTGA ATTTTTGCAG TTAAAGCATC GTGTAGTGTT TTTCTTAGTC CGTTACGTAG GTAGGAATCT GATGTAATGG TTGTTGGTAT TTTGTCACCA TTCATTTTTA TCTGGTTGTT CTCAAGTTCG GTTACGAGAT CCATTTGTCT ATCTAGTTCA ACTTGGAAAA TCAACGTATC AGTCGGGCGG CCTCGCTTAT CAACCACCAA TTTCATATTG CTGTAAGTGT TTAAATCTTT ACTTATTGGT TTCAAAACCC ATTGGTTAAG CCTTTTAAAC TCATGGTAGT TATTTTCAAG CATTAACATG AACTTAAATT CATCAAGGCT AATCTCTATA TTTGCCTTGT GAGTTTTCTT TTGTGTTAGT TCTTTTAATA ACCACTCATA AATCCTCATA GAGTATTTGT TTTCAAAAGA CTTAACATGT TCCAGATTAT ATTTTATGAA TTTTTTTAAC TGGAAAAGAT AAGGCAATAT CTCTTCACTA AAAACTAATT CTAATTTTTC GCTTGAGAAC TTGGCATAGT TTGTCCACTG GAAAATCTCA AAGCCTTTAA CCAAAGGATT CCTGATTTCC ACAGTTCTCG TCATCAGCTC TCTGGTTGCT TTAGCTAATA CACCATAAGC ATTTTCCCTA CTGATGTTCA TCATCTGAGC GTATTGGTTA TAAGTGAACG ATACCGTCCG TTCTTTCCTT GTAGGGTTTT CAATCGTGGG GTTGAGTAGT GCCACACAGC ATAAAATTAG CTTGGTTTCA TGCTCCGTTA AGTCATAGCG ACTAATCGCT AGTTCATTTG CTTTGAAAAC AACTAATTCA GACATACATC TCAATTGGTC TAGGTGATTT TAATCACTAT ACCAATTGAG ATGGGCTAGT CAATGATAAT TACTAGTCCT TTTCCTTTGA GTTGTGGGTA TCTGTAAATT CTGCTAGACC TTTGCTGGAA AACTTGTAAA TTCTGCTAGA CCCTCTGTAA ATTCCGCTAG ACCTTTGTGT GTTTTTTTTG TTTATATTCA AGTGGTTATA ATTTATAGAA TAAAGAAAGA ATAAAAAAAG ATAAAAAGAA TAGATCCCAG CCCTGTGTAT AACTCACTAC TTTAGTCAGT TCCGCAGTAT TACAAAAGGA TGTCGCAAAC GCTGTTTGCT CCTCTACAAA ACAGACCTTA AAACCCTAAA GGCTTAAGTA GCACCCTCGC AAGCTCGGTT GCGGCCGCAA TCGGGCAAAT CGCTGAATAT TCCTTTTGTC TCCGACCATC AGGCACCTGA GTCGCTGTCT TTTTCGTGAC ATTCAGTTCG CTGCGCTCAC GGCTCTGGCA GTGAATGGGG GTAAATGGCA CTACAGGCGC CTTTTATGGA TTCATGCAAG GAAACTACCC ATAATACAAG AAAAGCCCGT CACGGGCTTC TCAGGGCGTT TTATGGCGGG TCTGCTATGT GGTGCTATCT GACTTTTTGC TGTTCAGCAG TTCCTGCCCT CTGATTTTCC AGTCTGACCA CTTCGGATTA TCCCGTGACA GGTCATTCAG ACTGGCTAAT GCACCCAGTA AGGCAGCGGT ATCATCAACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTG
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