pHsp70-ALOX15(human)-EGFP
$84.00
In stock
SKU
PL79562
NM_001140.4;12-LOX;15-LOX;15-LOX-1;LOG15
100μg liquid plasmid(Instantly):$105
| name | pHsp70-ALOX15(human)-EGFP | another name | apHsp70-ALOX15(human)-EGFP |
| Insert Size (bp) | 1989bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | HSP70 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | SP6： | |
| 3′ sequencing primer | T7： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGGGTCTCT ACCGCATCCG CGTGTCCACT GGGGCCTCGC TCTATGCCGG TTCCAACAAC CAGGTGCAGC TGTGGCTGGT CGGCCAGCAC GGGGAGGCGG CGCTCGGGAA GCGACTGTGG CCCGCACGGG GCAAGGAGAC AGAACTCAAG GTGGAAGTAC CGGAGTATCT GGGGCCGCTG CTGTTTGTGA AACTGCGCAA ACGGCACCTC CTTAAGGACG ACGCCTGGTT CTGCAACTGG ATCTCTGTGC AGGGCCCCGG AGCCGGGGAC GAGGTCAGGT TCCCTTGTTA CCGCTGGGTG GAGGGCAACG GCGTCCTGAG CCTGCCTGAA GGCACCGGCC GCACTGTGGG CGAGGACCCT CAGGGCCTGT TCCAGAAACA CCGGGAAGAA GAGCTGGAAG AGAGAAGGAA GTTGTACCGG TGGGGAAACT GGAAGGACGG GTTAATTCTG AATATGGCTG GGGCCAAACT ATATGACCTC CCTGTGGATG AGCGATTTCT GGAAGACAAG AGAGTTGACT TTGAGGTTTC GCTGGCCAAG GGGCTGGCCG ACCTCGCTAT CAAAGACTCT CTAAATGTTC TGACTTGCTG GAAGGATCTA GATGACTTCA ACCGGATTTT CTGGTGTGGT CAGAGCAAGC TGGCTGAGCG CGTGCGGGAC TCCTGGAAGG AAGATGCCTT ATTTGGGTAC CAGTTTCTTA ATGGCGCCAA CCCCGTGGTG CTGAGGCGCT CTGCTCACCT TCCTGCTCGC CTAGTGTTCC CTCCAGGCAT GGAGGAACTG CAGGCCCAGC TGGAGAAGGA GCTGGAGGGA GGCACACTGT TCGAAGCTGA CTTCTCCCTG CTGGATGGGA TCAAGGCCAA CGTCATTCTC TGTAGCCAGC AGCACCTGGC TGCCCCTCTA GTCATGCTGA AATTGCAGCC TGATGGGAAA CTCTTGCCCA TGGTCATCCA GCTCCAGCTG CCCCGCACAG GATCCCCACC ACCTCCCCTT TTCTTGCCTA CGGATCCCCC AATGGCCTGG CTTCTGGCCA AATGCTGGGT GCGCAGCTCT GACTTCCAGC TCCATGAGCT GCAGTCTCAT CTTCTGAGGG GACACTTGAT GGCTGAGGTC ATTGTTGTGG CCACCATGAG GTGCCTGCCG TCGATACATC CTATCTTCAA GCTTATAATT CCCCACCTGC GATACACCCT GGAAATTAAC GTCCGGGCCA GGACTGGGCT GGTCTCTGAC ATGGGAATTT TCGACCAGAT AATGAGCACT GGTGGGGGAG GCCACGTGCA GCTGCTCAAG CAAGCTGGAG CCTTCCTAAC CTACAGCTCC TTCTGTCCCC CTGATGACTT GGCCGACCGG GGGCTCCTGG GAGTGAAGTC TTCCTTCTAT GCCCAAGATG CGCTGCGGCT CTGGGAAATC ATCTATCGGT ATGTGGAAGG AATCGTGAGT CTCCACTATA AGACAGACGT GGCTGTGAAA GACGACCCAG AGCTGCAGAC CTGGTGTCGA GAGATCACTG AAATCGGGCT GCAAGGGGCC CAGGACCGAG GGTTTCCTGT CTCTTTACAG GCTCGGGACC AGGTTTGCCA CTTTGTCACC ATGTGTATCT TCACCTGCAC CGGCCAACAC GCCTCTGTGC ACCTGGGCCA GCTGGACTGG TACTCTTGGG TGCCTAATGC ACCCTGCACG ATGCGGCTGC CCCCGCCAAC CACCAAGGAT GCAACGCTGG AGACAGTGAT GGCGACACTG CCCAACTTCC ACCAGGCTTC TCTCCAGATG TCCATCACTT GGCAGCTGGG CAGACGCCAG CCCGTTATGG TGGCTGTGGG CCAGCATGAG GAGGAGTATT TTTCGGGCCC TGAGCCTAAG GCTGTGCTGA AGAAGTTCAG GGAGGAGCTG GCTGCCCTGG ATAAGGAAAT TGAGATCCGG AATGCAAAGC TGGACATGCC CTACGAGTAC CTGCGGCCCA GCGTGGTGGA AAACAGTGTG GCCATCTAA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
