pCMV-SPORT6-PHLDB2
$84.00
In stock
SKU
PL79375
NM_001134439.1;LL5b;LL5beta
100μg liquid plasmid(Instantly):$105
| name | pCMV-SPORT6-PHLDB2 | another name | apCMV-SPORT6-PHLDB2 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-SPORT6 | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGATCTTGG GGACTATAGA CAGTTTGTAC AAAAAAGCAG GCTGGTACCG GTCCGGAATT CCCGGGATGA CGATTCCAGC AAGATTATGG AAGAGCATAG CTACATACAA AAGGAGCTAG ATTTACAAAA TGGTAGCTTA GAGGAAGACT CTGTGGTGCA TTCTGTTGAG AACGATTCCC AAAACATGAT GGAGAGCCTC AGCCCAAAGA AATACTCTTC CAGTCTGAGA TTTAAAGCCA ATGGAGACTA TTCTGGCTCC TATTTAACCC TCTCACAACC TGTGCCTGCA AAGAGAAGCC CTTCTCCTTT GGGAACCAGT GTCAGAAGCA GCCCCTCCTT AGCCAAAATC CAGGGAAGCA AGCAGTTCTC TTATGATGGA ACTGACAAAA ATATTCCTAT GAAACCTCCA ACTCCTTTAC TCAACACTAC ATCCTCCCTC AGTGGATATC CACTTGGAAG AGCAGACTTT GATCATTATA CTGGCCGGGA CAGTGAAAGG GCCTTGAGGC TCTCAGAGAA GCCTCCCTAT TCCAAATATA GCTCAAGGCA TAAATCGCAT GACAATGTCT ACTCTCTTGG AGGGCTGGAA GGTCGGAAGG CATCTGGCTC GCTCCTGGCC ATGTGGAATG GAAGTTCCCT GAGTGATGCT GGCCCGCCTC CTATCAGCAG ATCGGGAGCC GCAAGCATGC CTTCAAGCCC AAAGCAAGCC AGGAAAATGA GCATTCAGGA CAGCCTGGCG CTTCAACCCA AGTTAACTAG ACACAAGGAG CTTGCATCTG AAAACATCAA TTTGAGAACT AGGAAGTACT CCAGCAGCAG CCTGAGTCAC ATGGGAGCCT ACAGCCGATC ACTTCCCAGG TTGTACAGAG CCACAGAGAA CCAGCTGACA CCTCTCAGCT TGCCTCCAAG AAACTCTCTG GGCAATTCCA AACGAACAAA ACTTGGGGAA AAGGATCTAC CTCATAGCGT AATAGACAAT GACAATTACC TTAATTTTTC TTCTTTGAGC TCAGGGGCTT TACCCTATAA AACCTCTGCT TCTGAAGGCA ATCCTTATGT AAGTTCTACC CTCAGTGTCC CTGCCAGTCC ACGAGTGGCT CGGAAGATGC TTCTGGCCTC CACCTCCTCC TGTGCCTCTG ATGACTTTGA TCAGGCTTCA TATGTGGGGA CAAACCCGAG TCATTCACTT CTTGCTGGAG AGTCAGACAG AGTTTTTGCG ACCAGGAGGA ACTTCTCTTG TGGATCTGTG GAATTTGATG AGGCAGATTT GGAAAGCCTC AGACAGGCCT CAGGAACCCC CCAGCCTGCC CTTCGGGAAC GGAAAAGCAG TATTAGCTCC ATTTCAGGAC GTGATGACCT GATGGATTAT CACCGGCGGC AGAGGGAGGA AAGACTCAGG GAGCAGGAAA TGGAGCGATT GGAGAGACAG CGTCTGGAGA CCATCCTCAG TCTCTGTGCT GAATACACAA AGCCTGACAG TCGCTTATCT ACTGGGACCA CCGTGGAAGA TGTGCAGAAA ATCAACAAGG AGCTTGAGAA GCTGCAGCTC TCTGATGAGG AGTCTGTGTT TGAGGAAGCC CTCATGAGCC CTGACACAAG ATACAGGTGC CACCGGAAAG ACTCCCTCCC TGATGCAGAC TTGGCAAGCT GTGGGAGTCT CAGTCAGAGC AGTGCCAGCT TCTTTACCCC CAGGAGCACC AGGAATGATG AACTACTCAG TGACCTCACC CGGACTCCTC CACCACCATC CTCCACCTTT CCGAAAGCTT CCAGCGAGTC CTCTTATCTA AGTATCCTAC CAAAGACCCC AGAGGGTATA AGTGAAGAAC AGAGATCTCA GGAGTTGGCT GCAATGGAAG AAACCCGGAT AGTCATTCTG AACAACCTCG AGGAACTTAA GCAAAAAATC AAAGACATAA ATGATCAGAT GGATGAGTCT TTCAGAGAGT TGGATATGGA ATGTGCTCTT TTGGATGGAG AACAGAAATC TGAAACAACT GAACTTATGA AGGAGAAGGA GATTTTGGAT CATCTAAACC GGAAAATAGC TGAACTGGAA AAGAACATTG TTGGTGAAAA GACCAAGGAG AAGGTAAAGC TTGATGCTGA AAGGGAAAAA CTAGAGAGGC TTCAGGAGCT TTACTCCGAG CAGAAGACCC AGCTGGACAA TTGTCCTGAG TCCATGAGGG AACAGTTACA ACAACAACTG AAGAGGGATG CTGACCTGTT GGATGTTGAA AGCAAACACT TTGAAGACCT GGAGTTCCAG CAGCTTGAAC ATGAGAGCCG TCTAGATGAA GAAAAGGAGA ACTTGACTCA ACAGCTCCTG CGTGAAGTTG CTGAATATCA ACGGAACATC GTTTCTAGAA AGGAAAAAAT TTCTGCATTG AAAAAGCAAG CCAATCACAT TGTTCAGCAG GCTCAGAGAG AGCAAGATCA TTTTGTGAAA GAAAAGAATA ATTTAATAAT GATGTTGCAA AGAGAAAAGG AGAATCTTTG TAATTTGGAA AAGAAATACT CCAGCCTCTC TGGGGGGAAA GGGTTTCCCG TTAACCCCAA TACTTTAAAA GAGGGCTATA TCAGTGTAAA TGAGATTAAT GAGCCGTGTG GCAATTCCAC GAATCTATCC CCTTCCACTC AGTTTCCTGC TGATGCTGAT GCTGTTGCCA CTGAGCCTGC CACAGCTGTG CTGGCGAGCC AGCCACAGAG TAAAGAGCAC TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CATAAAGAAG GCCTCTATCT GAGTGATACT TTGCCTCGAA AGAAAACCAC ATCTTCCATC TCCCCACATT TCAGCAGTGC TACTATGGGG AGAAGCATCA CCCCAAAGGC CCATCTGCCC CTAGGACAGA GTAACAGCTG TGGAAGTGTG CTCCCTCCCT CACTGGCAGC CATGGCCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGT TATAATCACC AACAGATGAG TGAAGGACAC AGGCAGAAAT CTGAATTTTA TAACCGCACA GCATCTGAAT CAAATGTCTA CTTGAATAGT TTCCATTATC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTCGATAGC TCTGATAGCA TGGAGACCAG CATCTCTGCT TGCTCACCAG ACAACATCTC TAGTGCCAGC ACTTCAAATA TTGCTAGAAT AGAAGAAATG GAGAGACTTT TGAAGCAGGC TCATGCAGAA AAGACGCGGC TGCTCGAATC CAGGGAACGG GAAATGGAAG CCAAAAAACG AGCCCTGGAA GAAGAAAAAC GACGCCGGGA AATCCTGGAA AAACGATTAC AGGAAGAAAC TAGCCAGAGG CAGAAGTTAA TAGAAAAGGA AGTAAAAATA AGGGAGAGAC AAAGGGCACA GGCTCGTCCT TTGACACGCT ACCTGCCTGT CCGGAAGGAA GACTTTGATT TGCGGAGCCA TGTAGAGACT GCTGGCCACA ATATTGACAC CTGTTACCAT GTATCAATCA CAGAGAAGAC CTGCCGAGGA TTCCTCATCA AAATGGGTGG GAAAATTAAA ACGTGGAAAA AACGTTGGTT TGTTTTTGAT CGGAACAAGC GAACATTCTC TTATTATGCA GACAAGCATG AAACTAAATT GAAAGGAGTA ATATACTTTC AAGCCATTGA AGAAGTCTAT TATGATCACC TCAAGAATGC TAATAAGAGT CCTAATCCGT TACTCACCTT TAGCGTCAAG ACTCATGACA GAATCTATTA TATGGTAGCC CCATCGCCAG AAGCCATGCG GATCTGGATG GATGTTATAG TTACGGGGGC AGAAGGTTAC ACTCACTTCT TGTTGTAGTG AACTGAGGCA ACAGTCCACT TCAGGGCAGA CGGCAATAAT CTCTTACAAG AATGAAGCCA TATTCAACCC CAGATGAGAA AACCCAACAG ATCCATCCCT TGAGCTGTAA ACACTCAGAA CTCCTTTCAT ATCAAGACAA GTTATTTGTA AAAAATAAAG AAAGGGGTTT TAATACAAAC CTTCATAATA
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