pBARGPE1-Hygro-xyr1
$171.00
In stock
SKU
PL79340
100μg liquid plasmid(Instantly):$192
| name | pBARGPE1-Hygro-xyr1 | another name | apBARGPE1-Hygro-xyr1 |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | gpdA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCCCCGTCCG GTCCTGCCCG TCACCGAGAT CCACTTAACG TTACTGAAAT CATCAAACAG CTTGACGAAT CTGGATATAA GATCGTTGGT GTCGATGTCA GCTCCGGAGT TGAGACAAAT GGTGTTCAGG ATCTCGATAA GATACGTTCA TTTGTCCAAG CAGCAAAGAG TGCCTTCTAG TGATTTAATA GCTCCATGTC AACAAGAATA AAACGCGTTT CGGGTTTACC TCTTCCAGAT ACAGCTCATC TGCAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGTGTACGC ATGTAACATT ATACTGAAAA CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCAAGCTT TGAAGAAAAA TGCGCCTTAT TCAATCTTTG CTATAAAAAA TGGCCCAAAA TCTCACATTG GAAGACATTT GATGACCTCA TTTCTTTCAA TGAAGGGCCT AACGGAGTTG ACTAATGTTG TGGGAAATTG GAGCGATAAG CGTGCTTCTG CCGTGGCCAG GACAACGTAT ACTCATCAGA TAACAGCAAT ACCTGATCGA CCAATTCCGA TCATATTCAA TAACCCTTAA TATAACTTCG TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTA GCTTGGCTGC AGGTCGAGCG GCCGCGATCC GGAACCCTTA ATATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TCCCTCGACC GGCCGCGGCG GTTGTTTGCA AGACCGAGAA AAGGCTAGCA AGAATCGGGT CATTGTAGCG TATGCGCCTG TGAACATTCT CTTCAACAAG TTTGATTCCA TTGCGGTGAA ATGGTAAAAG TCAACCCCCT GCGATGTATA TTTTCCTGTA CAATCAATCA AAAAGCCAAA TGATTTAGCA TTATCTTTAC ATCTTGTTAT TTTACAGATT TTATGTTTAG ATCCACTAGT TTCTCGGTAC TATGCATTAC ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT ATATGATAAT CATCGCAAGA CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA TGTTTGAACG ATCTGCAGGT CGACGGACTA TTTCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA CTATCGGCGA GTACTTCTAC ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC GGCTCCGGAT CGGACGATTG CGTCGCATCG ACCCTGCGCC CAAGCTGCAT CATCGAAATT GCCGTCAACC AAGCTCTGAT AGAGTTGGTC AAGACCAATG CGGAGCATAT ACGCCCGGAG TCGTGGCGAT CCTGCAAGCT CCGGATGCCT CCGCTCGAAG TAGCGCGTCT GCTGCTCCAT ACAAGCCAAC CACGGCCTCC AGAAGAAGAT GTTGGCGACC TCGTATTGGG AATCCCCGAA CATCGCCTCG CTCCAGTCAA TGACCGCTGT TATGCGGCCA TTGTCCGTCA GGACATTGTT GGAGCCGAAA TCCGCGTGCA CGAGGTGCCG GACTTCGGGG CAGTCCTCGG CCCAAAGCAT CAGCTCATCG AGAGCCTGCG CGACGGACGC ACTGACGGTG TCGTCCATCA CAGTTTGCCA GTGATACACA TGGGGATCAG CAATCGCGCA TATGAAATCA CGCCATGTAG TGTATTGACC GATTCCTTGC GGTCCGAATG GGCCGAACCC GCTCGTCTGG CTAAGATCGG CCGCAGCGAT CGCATCCATA GCCTCCGCGA CCGGTTGTAG AACAGCGGGC AGTTCGGTTT CAGGCAGGTC TTGCAACGTG ACACCCTGTG CACGGCGGGA GATGCAATAG GTCAGGCTCT CGCTAAACTC CCCAATGTCA AGCACTTCCG GAATCGGGAG CGCGGCCGAT GCAAAGTGCC GATAAACATA ACGATCTTTG TAGAAACCAT CGGCGCAGCT ATTTACCCGC AGGACATATC CACGCCCTCC TACATCGAAG CTGAAAGCAC GAGATTCTTC GCCCTCCGAG AGCTGCATCA GGTCGGAGAC GCTGTCGAAC TTTTCGATCA GAAACTTCTC GACAGACGTC GCGGTGAGTT CAGGCTTTTT CATGGTTACT TCCTAATCGA AGCTTTGCTT GGGTAGAATA GGTAAGTCAG ATTGAATCTG AAATAAAGGG AGGAAGGGCG AACTTAAGAA GGTATGACCG GGTCGTTCAC TTACCTTGCT TGACAAACGC ACCAAGTTAT CGTGCACCAA GCAGCAGATG ATAATAATGT CCTCGTTCCT GTCTGCTAAT AAGAGTCACA CTTCGAGCGC CGCCGCTACT GCTACAAGTG GGGCTGATCT GACCAGTTGC CTAAATGAAC CATCTTGTCA AACGACACAA ATTTTGTGCT CACCGCCTGG ACGACTAAAC CAAAATAGGC ATTCATTGTT GACCTCCACT AGCTCCAGCC AAGCCCAAAA AGTGCTCCTT CAATATCATC TTCTGTCGAG TCTAGAGCAC ATTCTGCGGC CTCTGTGCTC TGCAAGCCGC AAACTTTCAC CAATGGACCA GAACTACCTG TGAAATTAAT AACAGACATA CTCCAAGCTG CCTTTGTGTG CTTAATCACG TATACTCACG TGCTCAATAG TCACCAATGC CCTCCCTCTT GGCCCTCTCC TTTTCTTTTT TCGACCGAAT TAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGAG ATCTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCGGA GAGACGGACG GACGCAGAGA GAAGGGCTGA GTAATAAGCG CCACTGCGCC AGACAGCTCT GGCGGCTCTG AGGTGCAGTG GATGATTATT AATCCGGGAC CGGCCGCCCC TCCGCCCCGA AGTGGAAAGG CTGGTGTGCC CCTCGTTGAC CAAGAATCTA TTGCATCATC GGAGAATATG GAGCTTCATC GAATCACCGG CAGTAAGCGA AGGAGAATGT GAAGCCAGGG GTGTATAGCC GTCGGCGAAA TAGCATGCCA TTAACCTAGG TACAGAAGTC CAATTGCTTC CGATCTGGTA AAAGATTCAC GAGATAGTAC CTTCTCCGAA GTAGGTAGAG CGAGTACCCG GCGCGTAAGC TCCCTAATTG GCCCATCCGG CATCTGTAGG GCGTCCAAAT ATCGTGCCTC TCCTGCTTTG CCCGGTGTAT GAAACCGGAA AGGCCGCTCA GGAGCTGGCC AGCGGCGCAG ACCGGGAACA CAAGCTGGCA GTCGACCCAT CCGGTGCTCT GCACTCGACC TGCTGAGGTC CCTCAGTCCC TGGTAGGCAG CTTTGCCCCG TCTGTCCGCC CGGTGTGTCG GCGGGGTTGA CAAGGTCGTT GCGTCAGTCC AACATTTGTT GCCATATTTT CCTGCTCTCC CCACCAGCTG CTCTTTTCTT TTCTCTTTCT TTTCCCATCT TCAGTATATT CATCTTCCCA TCCAAGAACC TTTAATCAAG CTTATCGATA CCGTCGACCT CGACTCTAGA GGATCCATGT TGTCCAACCC ATTGAGAAGA TACTCTGCCT ACCCAGACAT CTCCTCTGCT TCTTTCGACC CAAACTACCA CGGTTCTCAA TCTCACTTGC ACTCTATCAA CGTCAACACT TTCGGTAACT CTCACCCATA CCCAATGCAA CACTTGGCTC AACACGCTGA ATTGTCTTCT TCTAGAATGA TCAGAGCCTC TCCAGTTCAA CCAAAGCAAA GACAAGGTTC TTTGATTGCT GCCAGAAAGA ACTCTACTGG TACTGCTGGT CCAATTAGAA GAAGAATCTC TAGAGCTTGT GACCAATGTA ACCAATTGAG AACCAAGTGT GATGGTTTGC ACCCATGTGC CCACTGTATC GAATTTGGTT TGGGTTGTGA ATACGTCAGA GAAAGAAAGA AGAGAGGTAA GGCTTCTAGA AAGGATATTG CTGCCCAACA AGCCGCTGCT GCTGCTGCTC AACACTCCGG TCAAGTCCAA GATGGTCCAG AAGATCAACA CAGAAAGTTG TCTAGACAAC AATCTGAATC TTCTAGAGGT TCTGCTGAAT TGGCCCAACC AGCCCACGAC CCACCACACG GTCACATTGA AGGTTCTGTC TCTTCCTTCT CTGACAACGG TTTGTCCCAA CACGCTGCCA TGGGTGGTAT GGATGGTTTG GAAGATCACC ACGGTCACGT CGGTGTTGAT CCAGCCTTGG GTAGAACTCA ATTGGAAGCT TCTTCTGCTA TGGGTTTGGG TGCTTACGGT GAAGTCCACC CAGGTTACGA ATCTCCAGGT ATGAACGGTC ACGTTATGGT TCCACCATCT TACGGTGCTC AAACCACCAT GGCCGGTTAC TCCGGTATCT CTTACGCTGC TCAAGCCCCA TCTCCAGCTA CTTACTCTTC TGACGGTAAC TTCAGATTGA CCGGTCACAT CCACGATTAC CCATTGGCTA ACGGTTCTTC TCCATCTTGG GGTCAATCTG ATTTGAGATA CCCAGTTTTG GAACCATTGT TGCCACACTT GGGTAACATA CTCCCAGTGA GCTTGGCTTG TGATTTGATT GACTTGTACT TCTCCTCTTC TTCTTCTGCT CAAATGCACC CAATGTCCCC ATACGTTCTC GGCTTCGTCT TCAGAAAGAG ATCCTTCTTG CACCCAACTA ACCCAAGAAG ATGTCAACCA GCTTTGTTGG CTTCTATGTT GTGGGTTGCT GCTCAAACTT CTGAAGCTTC CTTCTTGACT TCTTTGCCAT CTGCTAGATC TAAGGTCTGT CAAAAGTTGT TGGAATTGAC CGTTGGTTTG TTGCAACCAT TGATCCACAC CGGTACCAAC TCTCCATCTC CAAAGACTTC TCCAGTCGTC GGTGCTGCTG CCTTGGGTGT TTTGGGTGTT GCCATGCCAG GTTCTCTCAA CATGGATAGC TTGGCCGGCG AAACTGGTGC GTTCGGTGCC ATAGGGAGCT TGGACGACGT CATAACCTAC GTTCACTTGG CCACTGTCGT CTCTGCCTCT GAATACAAGG GTGCCTCTTT GAGATGGTGG GGTGCTGCTT GGTCTTTGGC CAGAGAATTG AAGTTGGGTA GAGAATTGCC ACCAGGTAAC CCACCAGCCA ACCAAGAAGA CGGTGAAGGT TTGTCTGAAG ACGTTGATGA ACACGACTTG AACAGAAACA ACACTAGATT CGTTACTGAA GAAGAAAGAG AAGAAAGAAG AAGAGCTTGG TGGTTGGTTT ACATCGTCGA CAGACACTTG GCTTTGTGTT ACAACAGACC ATTGTTCTTG TTGGACTCTG AATGTTCTGA CTTGTACCAC CCAATGGACG ACATCAAGTG GCAAGCTGGT AAGTTCAGAT CTCACGATGC TGGTAACTCC TCTATCAACA TCGATTCTTC CATGACTGAC GAATTTGGTG ATTCTCCAAG AGCTGCTAGA GGTGCTCACT ACGAATGTAG AGGTAGATCT ATTTTCGGTT ACTTCTTGTC CTTGATGACT ATCTTGGGTG AAATTGTCGA TGTCCACCAC GCTAAGTCTC ACCCAAGATT TGGCGTTGGC TTCAGATCCG CTCGTGATTG GGACGAACAA GTTGCTGAAA TCACCAGACA CTTGGACATG TACGAAGAAT CTTTGAAGAG ATTCGTTGCC AAGCACTTGC CATTGTCCTC TAAGGACAAG GAACAACACG AAATGCACGA CTCTGGTGCT GTTACTGACA TGCAATCTCC ATTGTCTGTT AGAACCAACG CTTCCTCTAG AATGACTGAA TCTGAAATCC AAGCCTCTAT CGTTGTTGCT TACTCTACCC ACGTTATGCA CGTCTTGCAC ATCTTGTTGG CTGATAAGTG GGACCCAATC AACTTGTTGG ACGACGACGA CTTGTGGATC TCTTCTGAAG GTTTCGTTAC TGCTACTTCT CACGCTGTTA GTGCTGCGGA AGCTATAAGC CAAATTTTGG AATTTGACCC AGGTTTGGAA TTTATGCCAT TCTTCTACGG TGTCTACTTG TTGCAAGGTT CCTTCTTGTT GTTGTTGATC GCCGACAAGT TGCAAGCCGA AGCTTCTCCA TCTGTCATCA AGGCTTGTGA AACCATTGTT AGAGCTCACG AAGCTTGTGT TGTTACTTTG TCTACTGAAT ACCAAAGAAA CTTCTCTAAG GTTATGAGAT CTGCTTTGGC TTTGATTAGA GGTAGAGTTC CAGAAGATTT GGCTGAACAA CAACAAAGAA GAAGAGAATT GTTGGCTTTG TACAGATGGA CTGGTAACGG CACTGGTCTC GCCTTGTAAG AATTCGATAT CAAGCTTATC GATACCGTCG ACCTCGAGGG GGGGCCCGGT ACC
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