pCAMBIA1300-nLuc
$99.00
In stock
SKU
PL79216
100μg liquid plasmid(Instantly):$134
| name | pCAMBIA1300-nLuc | another name | apCAMBIA1300-nLuc |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | 35s | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCGGTCC CCAGATTAGC CTTTTCAATT TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGGT CTCATCAAGA CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACGAGCTCG GTACCCGGGA TCCTCTAGAG GGCCCGGCGC GCCACTAGTG TCGACCAGAT CTCGTACGCG TGGAGGAGGA GGAAGCGGAG GAGGAGGAAG CGAAGACGCC AAAAACATAA AGAAAGGCCC GGCGCCATTC TATCCGCTGG AAGATGGAAC CGCTGGAGAG CAACTGCATA AGGCTATGAA GAGATACGCC CTGGTTCCTG GAACAATTGC TTTTACAGAT GCACATATCG AGGTGGACAT CACTTACGCT GAGTACTTCG AAATGTCCGT TCGGTTGGCA GAAGCTATGA AACGATATGG GCTGAATACA AATCACAGAA TCGTCGTATG CAGTGAAAAC TCTCTTCAAT TCTTTATGCC GGTGTTGGGC GCGTTATTTA TCGGAGTTGC AGTTGCGCCC GCGAACGACA TTTATAATGA ACGTGAATTG CTCAACAGTA TGGGCATTTC GCAGCCTACC GTGGTGTTCG TTTCCAAAAA GGGGTTGCAA AAAATTTTGA ACGTGCAAAA AAAGCTCCCA ATCATCCAAA AAATTATTAT CATGGATTCT AAAACGGATT ACCAGGGATT TCAGTCGATG TACACGTTCG TCACATCTCA TCTACCTCCC GGTTTTAATG AATACGATTT TGTGCCAGAG TCCTTCGATA GGGACAAGAC AATTGCACTG ATCATGAACT CCTCTGGATC TACTGGTCTG CCTAAAGGTG TCGCTCTGCC TCATAGAACT GCCTGCGTGA GATTCTCGCA TGCCAGAGAT CCTATTTTTG GCAATCAAAT CATTCCGGAT ACTGCGATTT TAAGTGTTGT TCCATTCCAT CACGGTTTTG GAATGTTTAC TACACTCGGA TATTTGATAT GTGGATTTCG AGTCGTCTTA ATGTATAGAT TTGAAGAAGA GCTGTTTCTG AGGAGCCTTC AGGATTACAA GATTCAAAGT GCGCTGCTGG TGCCAACCCT ATTCTCCTTC TTCGCCAAAA GCACTCTGAT TGACAAATAC GATTTATCTA ATTTACACGA AATTGCTTCT GGTGGCGCTC CCCTCTCTAA GGAAGTCGGG GAAGCGGTTG CCAAGAGGTT CCATCTGCCA GGTATCAGGC AAGGATATGG GCTCACTGAG ACTACATCAG CTATTCTGAT TACACCCGAG GGGGATGATA AACCGGGCGC GGTCGGTAAA GTTGTTCCAT TTTTTGAAGC GAAGGTTGTG GATCTGGATA CCGGGAAAAC GCTGGGCGTT AATCAAAGAG GCGAACTGTG TGTGAGAGGT CCTATGATTA TGTCCGGTTA TGTAAACAAT CCGGAAGCGA CCAACGCCTT GATTGACAAG GATGGATGAC TGCAGAGCTT TCGTTCGTAT CATCGGTTTC GACAACGTTC GTCAAGTTCA ATGCATCAGT TTCATTGCGC ACACACCAGA ATCCTACTGA GTTCGAGTAT TATGGCATTG GGAAACATGT TTTTCTTGTA CCATTTGTTG TGCTTGTAAT TTACTGTGTT TTTTATTCGG TTTTCGCTAT CGAACTGTGA AATGGAAATG GATGGAGAAG AGTTAATGAA TGATATGGTC CTTTTGTTCA TTCTCAAATT AATATTATTT GTTTTTTCTC TTATTTGTTG TGTGTTGAAT TTGAAAATAT AAGAGATATG CAAACATTTT GTTTTGAGTA AAAATGTGTC AAATCGTGGC CTCTAATGAC CGAAGTTAAT ATGAGGAGTA AAACACTTGT AGTTGTACCA TTATGCTTAT TCACTAGGCA ACAAATATAT TTTCAGACCT AGAAAAGCTG CAAATGTTAC TGAATACAAG TATGTCCTCT TGTGTTTTAG ACATTTATGA ACTTTCCTTT ATGTAATTTT CCAGAATCCT TGTCAGATTC TAATCATTGC TTTATAATTA TAGTTATACT CATGGATTTG TAGTTGAGTA TGAAAATATT TTTTAATGCA TTTTATGACT TGCCAATTGA TTGACAACAT GCATCAAGCT TGGCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG CTAGAGCAGC TTGAGCTTGG ATCAGATTGT CGTTTCCCGC CTTCAGTTTA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAACGG ATATTTAAAA GGGCGTGAAA AGGTTTATCC GTTCGTCCAT TTGTATGTGC ATGCCAACCA CAGGGTTCCC CTCGGGATCA AAGTACTTTG ATCCAACCCC TCCGCTGCTA TAGTGCAGTC GGCTTCTGAC GTTCAGTGCA GCCGTCTTCT GAAAACGACA TGTCGCACAA GTCCTAAGTT ACGCGACAGG CTGCCGCCCT GCCCTTTTCC TGGCGTTTTC TTGTCGCGTG TTTTAGTCGC ATAAAGTAGA ATACTTGCGA CTAGAACCGG AGACATTACG CCATGAACAA GAGCGCCGCC GCTGGCCTGC TGGGCTATGC CCGCGTCAGC ACCGACGACC AGGACTTGAC CAACCAACGG GCCGAACTGC ACGCGGCCGG CTGCACCAAG CTGTTTTCCG AGAAGATCAC CGGCACCAGG CGCGACCGCC CGGAGCTGGC CAGGATGCTT GACCACCTAC GCCCTGGCGA CGTTGTGACA GTGACCAGGC TAGACCGCCT GGCCCGCAGC ACCCGCGACC TACTGGACAT TGCCGAGCGC ATCCAGGAGG CCGGCGCGGG CCTGCGTAGC CTGGCAGAGC CGTGGGCCGA CACCACCACG CCGGCCGGCC GCATGGTGTT GACCGTGTTC GCCGGCATTG CCGAGTTCGA GCGTTCCCTA ATCATCGACC GCACCCGGAG CGGGCGCGAG GCCGCCAAGG CCCGAGGCGT GAAGTTTGGC CCCCGCCCTA CCCTCACCCC GGCACAGATC GCGCACGCCC GCGAGCTGAT CGACCAGGAA GGCCGCACCG TGAAAGAGGC GGCTGCACTG CTTGGCGTGC ATCGCTCGAC CCTGTACCGC GCACTTGAGC GCAGCGAGGA AGTGACGCCC ACCGAGGCCA GGCGGCGCGG TGCCTTCCGT GAGGACGCAT TGACCGAGGC CGACGCCCTG GCGGCCGCCG AGAATGAACG CCAAGAGGAA CAAGCATGAA ACCGCACCAG GACGGCCAGG ACGAACCGTT TTTCATTACC GAAGAGATCG AGGCGGAGAT GATCGCGGCC GGGTACGTGT TCGAGCCGCC CGCGCACGTC TCAACCGTGC GGCTGCATGA AATCCTGGCC GGTTTGTCTG ATGCCAAGCT GGCGGCCTGG CCGGCCAGCT TGGCCGCTGA AGAAACCGAG CGCCGCCGTC TAAAAAGGTG ATGTGTATTT GAGTAAAACA GCTTGCGTCA TGCGGTCGCT GCGTATATGA TGCGATGAGT AAATAAACAA ATACGCAAGG GGAACGCATG AAGGTTATCG CTGTACTTAA CCAGAAAGGC GGGTCAGGCA AGACGACCAT CGCAACCCAT CTAGCCCGCG CCCTGCAACT CGCCGGGGCC GATGTTCTGT TAGTCGATTC CGATCCCCAG GGCAGTGCCC GCGATTGGGC GGCCGTGCGG GAAGATCAAC CGCTAACCGT TGTCGGCATC GACCGCCCGA CGATTGACCG CGACGTGAAG GCCATCGGCC GGCGCGACTT CGTAGTGATC GACGGAGCGC CCCAGGCGGC GGACTTGGCT GTGTCCGCGA TCAAGGCAGC CGACTTCGTG CTGATTCCGG TGCAGCCAAG CCCTTACGAC ATATGGGCCA CCGCCGACCT GGTGGAGCTG GTTAAGCAGC GCATTGAGGT CACGGATGGA AGGCTACAAG CGGCCTTTGT CGTGTCGCGG GCGATCAAAG GCACGCGCAT CGGCGGTGAG GTTGCCGAGG CGCTGGCCGG GTACGAGCTG CCCATTCTTG AGTCCCGTAT CACGCAGCGC GTGAGCTACC CAGGCACTGC CGCCGCCGGC ACAACCGTTC TTGAATCAGA ACCCGAGGGC GACGCTGCCC GCGAGGTCCA GGCGCTGGCC GCTGAAATTA AATCAAAACT CATTTGAGTT AATGAGGTAA AGAGAAAATG AGCAAAAGCA CAAACACGCT AAGTGCCGGC CGTCCGAGCG CACGCAGCAG CAAGGCTGCA ACGTTGGCCA GCCTGGCAGA CACGCCAGCC ATGAAGCGGG TCAACTTTCA GTTGCCGGCG GAGGATCACA CCAAGCTGAA GATGTACGCG GTACGCCAAG GCAAGACCAT TACCGAGCTG CTATCTGAAT ACATCGCGCA GCTACCAGAG TAAATGAGCA AATGAATAAA TGAGTAGATG AATTTTAGCG GCTAAAGGAG GCGGCATGGA AAATCAAGAA CAACCAGGCA CCGACGCCGT GGAATGCCCC ATGTGTGGAG GAACGGGCGG TTGGCCAGGC GTAAGCGGCT GGGTTGTCTG CCGGCCCTGC AATGGCACTG GAACCCCCAA GCCCGAGGAA TCGGCGTGAC GGTCGCAAAC CATCCGGCCC GGTACAAATC GGCGCGGCGC TGGGTGATGA CCTGGTGGAG AAGTTGAAGG CCGCGCAGGC CGCCCAGCGG CAACGCATCG AGGCAGAAGC ACGCCCCGGT GAATCGTGGC AAGCGGCCGC TGATCGAATC CGCAAAGAAT CCCGGCAACC GCCGGCAGCC GGTGCGCCGT CGATTAGGAA GCCGCCCAAG GGCGACGAGC AACCAGATTT TTTCGTTCCG ATGCTCTATG ACGTGGGCAC CCGCGATAGT CGCAGCATCA TGGACGTGGC CGTTTTCCGT CTGTCGAAGC GTGACCGACG AGCTGGCGAG GTGATCCGCT ACGAGCTTCC AGACGGGCAC GTAGAGGTTT CCGCAGGGCC GGCCGGCATG GCCAGTGTGT GGGATTACGA CCTGGTACTG ATGGCGGTTT CCCATCTAAC CGAATCCATG AACCGATACC GGGAAGGGAA GGGAGACAAG CCCGGCCGCG TGTTCCGTCC ACACGTTGCG GACGTACTCA AGTTCTGCCG GCGAGCCGAT GGCGGAAAGC AGAAAGACGA CCTGGTAGAA ACCTGCATTC GGTTAAACAC CACGCACGTT GCCATGCAGC GTACGAAGAA GGCCAAGAAC GGCCGCCTGG TGACGGTATC CGAGGGTGAA GCCTTGATTA GCCGCTACAA GATCGTAAAG AGCGAAACCG GGCGGCCGGA GTACATCGAG ATCGAGCTAG CTGATTGGAT GTACCGCGAG ATCACAGAAG GCAAGAACCC GGACGTGCTG ACGGTTCACC CCGATTACTT TTTGATCGAT CCCGGCATCG GCCGTTTTCT CTACCGCCTG GCACGCCGCG CCGCAGGCAA GGCAGAAGCC AGATGGTTGT TCAAGACGAT CTACGAACGC AGTGGCAGCG CCGGAGAGTT CAAGAAGTTC TGTTTCACCG TGCGCAAGCT GATCGGGTCA AATGACCTGC CGGAGTACGA TTTGAAGGAG GAGGCGGGGC AGGCTGGCCC GATCCTAGTC ATGCGCTACC GCAACCTGAT CGAGGGCGAA GCATCCGCCG GTTCCTAATG TACGGAGCAG ATGCTAGGGC AAATTGCCCT AGCAGGGGAA AAAGGTCGAA AAGGTCTCTT TCCTGTGGAT AGCACGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGAACCCGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGTCACAC ATGTAAGTGA CTGATATAAA AGAGAAAAAA GGCGATTTTT CCGCCTAAAA CTCTTTAAAA CTTATTAAAA CTCTTAAAAC CCGCCTGGCC TGTGCATAAC TGTCTGGCCA GCGCACAGCC GAAGAGCTGC AAAAAGCGCC TACCCTTCGG TCGCTGCGCT CCCTACGCCC CGCCGCTTCG CGTCGGCCTA TCGCGGCCGC TGGCCGCTCA AAAATGGCTG GCCTACGGCC AGGCAATCTA CCAGGGCGCG GACAAGCCGC GCCGTCGCCA CTCGACCGCC GGCGCCCACA TCAAGGCACC CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GCATTCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC TGTTCTTCCC CGATATCCTC CCTGATCGAC CGGACGCAGA AGGCAATGTC ATACCACTTG TCCGCCCTGC CGCTTCTCCC AAGATCAATA AAGCCACTTA CTTTGCCATC TTTCACAAAG ATGTTGCTGT CTCCCAGGTC GCCGTGGGAA AAGACAAGTT CCTCTTCGGG CTTTTCCGTC TTTAAAAAAT CATACAGCTC GCGCGGATCT TTAAATGGAG TGTCTTCTTC CCAGTTTTCG CAATCCACAT CGGCCAGATC GTTATTCAGT AAGTAATCCA ATTCGGCTAA GCGGCTGTCT AAGCTATTCG TATAGGGACA ATCCGATATG TCGATGGAGT GAAAGAGCCT GATGCACTCC GCATACAGCT CGATAATCTT TTCAGGGCTT TGTTCATCTT CATACTCTTC CGAGCAAAGG ACGCCATCGG CCTCACTCAT GAGCAGATTG CTCCAGCCAT CATGCCGTTC AAAGTGCAGG ACCTTTGGAA CAGGCAGCTT TCCTTCCAGC CATAGCATCA TGTCCTTTTC CCGTTCCACA TCATAGGTGG TCCCTTTATA CCGGCTGTCC GTCATTTTTA AATATAGGTT TTCATTTTCT CCCACCAGCT TATATACCTT AGCAGGAGAC ATTCCTTCCG TATCTTTTAC GCAGCGGTAT TTTTCGATCA GTTTTTTCAA TTCCGGTGAT ATTCTCATTT TAGCCATTTA TTATTTCCTT CCTCTTTTCT ACAGTATTTA AAGATACCCC AAGAAGCTAA TTATAACAAG ACGAACTCCA ATTCACTGTT CCTTGCATTC TAAAACCTTA AATACCAGAA AACAGCTTTT TCAAAGTTGT TTTCAAAGTT GGCGTATAAC ATAGTATCGA CGGAGCCGAT TTTGAAACCG CGGTGATCAC AGGCAGCAAC GCTCTGTCAT CGTTACAATC AACATGCTAC CCTCCGCGAG ATCATCCGTG TTTCAAACCC GGCAGCTTAG TTGCCGTTCT TCCGAATAGC ATCGGTAACA TGAGCAAAGT CTGCCGCCTT ACAACGGCTC TCCCGCTGAC GCCGTCCCGG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGA ATTAACGCCG AATTAATTCG GGGGATCTGG ATTTTAGTAC TGGATTTTGG TTTTAGGAAT TAGAAATTTT ATTGATAGAA GTATTTTACA AATACAAATA CATACTAAGG GTTTCTTATA TGCTCAACAC ATGAGCGAAA CCCTATAGGA ACCCTAATTC CCTTATCTGG GAACTACTCA CACATTATTA TGGAGAAACT CGAGCTTGTC GATCGACAGA TCCGGTCGGC ATCTACTCTA TTTCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA CTATCGGCGA GTACTTCTAC ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC GGCTCCGGAT CGGACGATTG CGTCGCATCG ACCCTGCGCC CAAGCTGCAT CATCGAAATT GCCGTCAACC AAGCTCTGAT AGAGTTGGTC AAGACCAATG CGGAGCATAT ACGCCCGGAG TCGTGGCGAT CCTGCAAGCT CCGGATGCCT CCGCTCGAAG TAGCGCGTCT GCTGCTCCAT ACAAGCCAAC CACGGCCTCC AGAAGAAGAT GTTGGCGACC TCGTATTGGG AATCCCCGAA CATCGCCTCG CTCCAGTCAA TGACCGCTGT TATGCGGCCA TTGTCCGTCA GGACATTGTT GGAGCCGAAA TCCGCGTGCA CGAGGTGCCG GACTTCGGGG CAGTCCTCGG CCCAAAGCAT CAGCTCATCG AGAGCCTGCG CGACGGACGC ACTGACGGTG TCGTCCATCA CAGTTTGCCA GTGATACACA TGGGGATCAG CAATCGCGCA TATGAAATCA CGCCATGTAG TGTATTGACC GATTCCTTGC GGTCCGAATG GGCCGAACCC GCTCGTCTGG CTAAGATCGG CCGCAGCGAT CGCATCCATA GCCTCCGCGA CCGGTTGTAG AACAGCGGGC AGTTCGGTTT CAGGCAGGTC TTGCAACGTG ACACCCTGTG CACGGCGGGA GATGCAATAG GTCAGGCTCT CGCTAAACTC CCCAATGTCA AGCACTTCCG GAATCGGGAG CGCGGCCGAT GCAAAGTGCC GATAAACATA ACGATCTTTG TAGAAACCAT CGGCGCAGCT ATTTACCCGC AGGACATATC CACGCCCTCC TACATCGAAG CTGAAAGCAC GAGATTCTTC GCCCTCCGAG AGCTGCATCA GGTCGGAGAC GCTGTCGAAC TTTTCGATCA GAAACTTCTC GACAGACGTC GCGGTGAGTT CAGGCTTTTT CATATCTCAT TGCCCCCCGG GATCTGCGAA AGCTCGAGAG AGATAGATTT GTAGAGAGAG ACTGGTGATT TCAGCGTGTC CTCTCCAAAT GAAATGAACT TCCTTATATA GAGGAAGGTC TTGCGAAGGA TAGTGGGATT GTGCGTCATC CCTTACGTCA GTGGAGATAT CACATCAATC CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTGA ACGATAGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG TGCCACCTTC CTTTTCTACT GTCCTTTTGA TGAAGTGACA GATAGCTGGG CAATGGAATC CGAGGAGGTT TCCCGATATT ACCCTTTGTT GAAAAGTCTC AATAGCCCTT TGGTCTTCTG AGACTGTATC TTTGATATTC TTGGAGTAGA CGAGAGTGTC GTGCTCCACC ATGTTATCAC ATCAATCCAC TTGCTTTGAA GACGTGGTTG GAACGTCTTC TTTTTCCACG ATGCTCCTCG TGGGTGGGGG TCCATCTTTG GGACCACTGT CGGCAGAGGC ATCTTGAACG ATAGCCTTTC CTTTATCGCA ATGATGGCAT TTGTAGGTGC CACCTTCCTT TTCTACTGTC CTTTTGATGA AGTGACAGAT AGCTGGGCAA TGGAATCCGA GGAGGTTTCC CGATATTACC CTTTGTTGAA AAGTCTCAAT AGCCCTTTGG TCTTCTGAGA CTGTATCTTT GATATTCTTG GAGTAGACGA GAGTGTCGTG CTCCACCATG TTGGCAAGCT GCTCTAGCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACG
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