pDSK-GFPuv-Cam
$128.00
In stock
SKU
PL79199
100μg liquid plasmid(Instantly):$163
| name | pDSK-GFPuv-Cam | another name | apDSK-GFPuv-Cam |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Chl | Fluorescent labeling | Green |
| Promoter | Replicon | RSF1010 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC TGGTATGAGT CAGCAACACC TTCTTCACGA GGCAGACCTC AGCGCTATTC TGACCTTGCC ATCACGACTG TGCTGGTCAT TAAACGCGTA TTCAGGCTGA CCCTGCGCGC TGCGCAGGGC TTTATTGATT CCATTTTTAC ACTGATGAAT GTTCCGTTGC GCTGCCCGGA TTACAGCTGC GTTTCCTGTT TGGGGTCGTT TGCGGGAAGG GGCGGAATCC TACGCTAAGG CTTTGGCCAG CGATATTCTC CGGTGAGATT GATGTGTTCC CAGGGGATAG GAGAAGTCGC TTGATATCTA GTATGACGTC TGTCGCACCT GCTTGATCGC GGCCGCGATA GCTAGATCGC GTTGCTCCTC TTCTCCATCC GCGTTCCAAG CTGCGGAAAG GCACCCATAA GCGTACGCCT GGTCGAGCAG GCGACGCGGA TCGACGTCCA GCGCACGAGA GAATGCGTCC GCCATCTGTG CAATGCGTCT AGGATCGAGA CAAAGGTCGT CTCTGTCAGC CGGATCGTAG AACATATTGG CGGCGCCAAA GCCCACTTCA CCGACCAGAC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CTTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAGACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAGGTACC CGGGGATCTA GAGGATCTCA ATGAATATTG GTTGACACGG GCGTATAAGA CATGTTATAC TGTTGAATAA CAAGCTTTCA ATTCTCTATT TTGAGAAGAA TTAATGTGCT TGGGAGTCCC TGATGATTAA ATTAAATAAA CCAAGATTTT ACCATGACTG CAATTTTAGA GAGACGGGGG ATCGATCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCATG GCCAACACTT GTCACTACTT TCTCTTATGG TGTTCAATGC TTTTCCCGTT ATCCGGATCA TATGAAACGG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAACGCAC TATATCTTTC AAAGATGACG GGAACTACAA GACGCGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATCGTATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT CGGACACAAA CTCGAGTACA ACTATAACTC ACACAATGTA TACATCACGG CAGACAAACA AAAGAATGGA ATCAAAGCTA ACTTCAAAAT TCGCCACAAC ATTGAAGATG GATCAGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCGACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGCGTGACCA CATGGTCCTT CTTGAGTTTG TAACTGCTGC TGGGATTACA CATGGCATGG ATGAGCTCTA CAAATAAGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAAGA AGAGCATACA TCTGGAAGCA AAGCCAGGAA AGCGGCCTAT GGAGCTGTGC GGCAGCGCTC AGTAGGCAAT TTTTCAAAAT ATTGTTAAGC CTTTTCTGAG CATGGTATTT TTCATGGTAT TACCAATTAG CAGGAAAATA AGCCATTGAA TATAAAAGAT AAAAATGTCT TGTTTACAAT AGAGTGGGGG GGGTCAGCCT GCCGCCTTGG GCCGGGTGAT GTCGTACTTG CCCGCCGCGA ACTCGGTTAC CGTCCAGCCC AGCGCGACCA GCTCCGGCAA CGCCTCGCGC ACCCGCTGGC GGCGCTTGCG CATGGTCGAA CCACTGGCCT CTGACGGCCA GACATAGCCG CACAAGGTAT CTATGGAAGC CTTGCCGGTT TTGCCGGGGT CGATCCAGCC ACACAGCCGC TGGTGCAGCA GGCGGGCGGT TTCGCTGTCC AGCGCCCGCA CCTCGTCCAT GCTGATGCGC ACATGCTGGC CGCCACCCAT GACGGCCTGC GCGATCAAGG GGTTCAGGGC CACGTACAGG CGCCCGTCCG CCTCGTCGCT GGCGTACTCC GACAGCAGCC GAAACCCCTG CCGCTTGCGG CCATTCTGGG CGATGATGGA TACCTTCCAA AGGCGCTCGA TGCAGTCCTG TATGTGCTTG AGCGCCCCAC CACTATCGAC CTCTGCCCCG ATTTCCTTTG CCAGCGCCCG ATAGCTACCT TTGACCACCA TGGCATCAGC GGTGACGGCC TCCCACTTGG GTTCCAGGAA CAGCCGGAGC TGCCGTCCGC CTTCGGTCTT GGGTTCCGGG CCAAGCACTA GGCCATTAGG CCCAGCCATG GCCACCAGCC CTTGCAGGAT GCGCAGATCA TCAGCGCCCA GCGGCTCCGG GCCGCTGAAC TCGATCCGCT TGCCGTCGCC GTAGTCATAC GTCACGTCCA GCTTGCTGCG CTTGCGCTCG CCCCGCTTGA GGGCACGGAA CAGGCCGGGG GCCAGACAGT GCGCCGGGTC GTGCCGGACG TGGCTGAGGC TGTGCTTGTT CTTAGGCTTC ACCACGGGGC ACCCCCTTGC TCTTGCGCTG CCTCTCCAGC ACGGCGGGCT TGAGCACCCC GCCGTCATGC CGCCTGAACC ACCGATCAGC GAACGGTGCG CCATAGTTGG CCTTGCTCAC ACCGAAGCGG ACGAAGAACC GGCGCTGGTC GTCGTCCACA CCCCATTCCT CGGCCTCGGC GCTGGTCATG CTCGACAGGT AGGACTGCCA GCGGATGTTA TCGACCAGTA CCGAGCTGCC CCGGCTGGCC TGCTGCTGGT CGCCTGCGCC CATCATGGCC GCGCCCTTGC TGGCATGGTG CAGGAACACG ATAGAGCACC CGGTATCGGC GGCGATGGCC TCCATGCGAC CGATGACCTG GGCCATGGGG CCGCTGGCGT TTTCTTCCTC GATGTGGAAC CGGCGCAGCG TGTCCAGCAC CATCAGGCGG CGGCCCTCGG CGGCGCGCTT GAGGCCGTCG AACCACTCCG GGGCCATGAT GTTGGGCAGG CTGCCGATCA GCGGCTGGAT CAGCAGGCCG TCAGCCACGG CTTGCCGTTC CTCGGCGCTG AGGTGCGCCC CAAGGGCGTG CAGGCGGTGA TGAATGGCGG TGGGCGGGTC TTCGGCGGGC AGGTAGATCA CCGGGCCGGT GGGCAGTTCG CCCACCTCCA GCAGATCCGG CCCGCCTGCA ATCTGTGCGG CCAGTTGCAG GGCCAGCATG GATTTACCGG CACCACCGGG CGACACCAGC GCCCCGACCG TACCGGCCAC CATGTTGGGC AAAACGTAGT CCAGCGGTGG CGGCGCTGCT GCGAACGCCT CCAGAATATT GATAGGCTTA TGGGTAGCCA TTGATTGCCT CCTTTGCAGG CAGTTGGTGG TTAGGCGCTG GCGGGGTCAC TACCCCCGCC CTGCGCCGCT CTGAGTTCTT CCAGGCACTC GCGCAGCGCC TCGTATTCGT CGTCGGTCAG CCAGAACTTG CGCTGACGCA TCCCTTTGGC CTTCATGCGC TCGGCATATC GCGCTTGGCG TACAGCGTCA GGGCTGGCCA GCAGGTCGCC GGTCTGCTTG TCCTTTTGGT CTTTCATATC AGTCACCGAG AAACTTGCCG GGGCCGAAAG GCTTGTCTTC GCGGAACAAG GACAAGGTGC AGCCGTCAAG GTTAAGGCTG GCCATATCAG CGACTGAAAA GCGGCCAGCC TCGGCCTTGT TTGACGTATA ACCAAAGCCA CCGGGCAACC AATAGCCCTT GTCACTTTTG ATCAGGTAGA CCGACCCTGA AGCGCTTTTT TCGTATTCCA TAAAACCCCC TTCTGTGCGT GAGTACTCAT AGTATAACAG GCGTGAGTAC CAACGCAAGC ACTACATGCT GAAATCTGGC CCGCCCCTGT CCATGCCTCG CTGGCGGGGT GCCGGTGCCC GTGCCAGCTC GGCCCGCGCA AGCTGGACGC TGGGCAGACC CATGACCTTG CTGACGGTGC GCTCGATGTA ATCCGCTTCG TGGCCGGGCT TGCGCTCTGC CAGCGCTGGG CTGGCCTCGG CCATGGCCTT GCCGATTTCC TCGGCACTGC GGCCCCGGCT GGCCAGCTTC TGCGCGGCGA TAAAGTCGCA CTTGCTGAGG TCATCACCGA AGCGCTTGAC CAGCCCGGCC ATCTCGCTGC GGTACTCGTC CAGCGCCGTG CGCCGGTGGC GGCTAAGCTG CCGCTCGGGC AGTTCGAGGC TGGCCAGCCT GCGGGCCTTC TCCTGCTGCC GCTGGGCCTG CTCGATCTGC TGGCCAGCCT GCTGCACCAG CGCCGGGCCA GCGGTGGCGG TCTTGCCCTT GGATTCACGC AGCAGCACCC ACGGCTGATA ACCGGCGCGG GTGGTGTGCT TGTCCTTGCG GTTGGTGAAG CCCGCCAAGC GGCCATAGTG GCGGCTGTCG GCGCTGGCCG GGTCGGCGTC GTACTCGCTG GCCAGCGTCC GGGCAATCTG CCCCCGAAGT TCACCGCCTG CGGCGTCGGC CACCTTGACC CATGCCTGAT AGTTCTTCGG GCTGGTTTCC ACTACCAGGG CAGGCTCCCG GCCCTCGGCT TTCATGTCAT CCAGGTCAAA CTCGCTGAGG TCGTCCACCA GCACCAGACC ATGCCGCTCC TGCTCGGCGG GCCTGATATA CACGTCATTG CCCTGGGCAT TCATCCGCTT GAGCCATGGC GTGTTCTGGA GCACTTCGGC GGCTGACCAT TCCCGGTTCA TCATCTGGCC GGTGGTGGCG TCCCTGACGC CGATATCGAA GCGCTCACAG CCCATGGCCT TGAGCTGTCG GCCTATGGCC TGCAAAGTCC TGTCGTTCTT CATCGGGCCA CCAAGCGCAG CCAGATCGAG CCGTCCTCGG TTGTCAGTGG CGTCAGGTCG AGCAAGAGCA ACGATGCGAT CAGCAGCACC ACCGTAGGCA TCATGGAAGC CAGCATCACG GTTAGCCATA GCTTCCAGTG CCACCCCCGC GACGCGCTCC GGGCGCTCTG CGCGGCGCTG CTCACCTCGG CGGCTACCTC CCGCAACTCT TTGGCCAGCT CCACCCATGC CGCCCCTGTC TGGCGCTGGG CTTTCAGCCA CTCCGCCGCC TGCGCCTCGC TGGCCTGCTG GGTCTGGCTC ATGACCTGCC GGGCTTCGTC GGCCAGTGTC GCCATGCTCT GGGCCAGCGG TTCGATCTGC TCCGCTAACT CGTTGATGCC TCTGGATTTC TTCACTCTGT CGATTGCGTT CATGGTCTAT TGCCTCCCGG TATTCCTGTA AGTCGATGAT CTGGGCGTTG GCGGTGTCGA TGTTCAGGGC CACGTCTGCC CGGTCGGTGC GGATGCCCCG GCCTTCCATC TCCACCACGT TCGGCCCCAG GTGAACACCG GGCAGGCGCT CGATGCCCTG CGCCTCAAGT GTTCTGTGGT CAATGCGGGC GTCGTGGCCA GCCCGCTCTA ATGCCCGGTT GGCATGGTCG GCCCATGCCT CGCGGGTCTG CTCAAGCCAT GCCTTGGGCT TGAGCGCTTC GGTCTTCTGT GCCCCGCCCT TCTCCGGGGT CTTGCCGTTG TACCGCTTGA ACCACTGAGC GGCGGGCCGC TCGATGCCGT CATTGATCCG CTCGGAGATC ATCAGGTGGC AGTGCGGGTT CTCGCCGCCA CCGGCATGGA TGGCCAGCGT ATACGGCAGG CGCTCGGCAC CGGTCAGGTG CTGGGCGAAC TCGGACGCCA GCGCCTTCTG CTGGTCGAGG GTCAGCTCGA CCGGCAGGGC AAATTCGACC TCCTTGAACA GCCGCCCATT GGCGCGTTCA TACAGGTCGG CAGCATCCCA GTAGTCGGCG GGCCGCTCGA CGAACTCCGG CATGTGCCCG GATTCGGCGT GCAAGACTTC ATCCATGTCG CGGGCATACT TGCCTTCGCG CTGGATGTAG TCGGCCTTGG CCCTGGCCGA TTGGCCGCCC GACCTGCTGC CGGTTTTCGC CGTAAGGTGA TAAATCGCCA TGCTGCCTCG CTGTTGCTTT TGCTTTTCGG CTCCATGCAA TGGCCCTCGG AGAGCGCACC GCCCGAAGGG TGGCCGTTAG GCCAGTTTCT CGAAGAGAAA CCGGTAAGTG CGCCCTCCCC TACAAAGTAG GGTCGGGATT GCCGCCGCTG TGCCTCCATG ATAGCCTACG AGACAGCACA TTAACAATGG GGTGTCAAGA TGGTTAAGGG GAGCAACAAG GCGGCGGATC GGCTGGCCAA GCTCGAAGAA CAACGAGCGC GAATCAATGC CGAAATTCAG CGGGTGCGGG CAAGGGAACA GCAGCAAGAG CGCAAGAACG AAACAAGGCG CAAGGTGCTG GTGGGGGCCA TGATTTTGGC CAAGGTGAAC AGCAGCGAGT GGCCGGAGGA TCGGCTCATG GCGGCAATGG ATGCGTACCT TGAACGCGAC CACGACCGCG CCTTGTTCGG TCTGCCGCCA CGCCAGAAGG ATGAGCCGGG CTGAATGATC GACCGAGACA GGCCCTGCGG GGCTGCACAC GCGCCCCCAC CCTTCGGGTA GGGGGAAAGG CCGCTAAAGC GGCTAAAAGC GCTCCAGCGT ATTTCTGCGG GGTTTGGTGT GGGGTTTAGC GGGCTTTGCC CGCCTTTCCC CCTGCCGCGC AGCGGTGGGG CGGTGTGTAG CCTAGCGCAG CGAATAGACC AGCTATCCGG CCTCTGGCCG GGCATATTGG GCAAGGGCAG CAGCGCCCCA CAAGGGCGCT GATAACCGCG CCTAGTGGAT TATTCTTAGA TAATCATGGA TGGATTTTTC CAACACCCCG CCAGCCCCCG CCCCTGCTGG GTTTGCAGGT TTGGGGGCGT GACAGTTATT GCAGGGGTTC GTGACAGTTA TTGCAGGGGG GCGTGACAGT TATTGCAGGG GTTCGTGACA GTTAGTACGG GAGTGACGGG CACTGGCTGG CAATGTCTAG CAACGGCAGG CATTTCGGCT GAGGGTAAAA GAACTTTCCG CTAAGCGATA GACTGTATGT AAACACAGTA TTGCAAGGAC GCGGAACATG CCTCATGTGG CGGCCAGGAC GGCCAGCCGG GATCGGGATA CTGGTCGTTA CCAGAGCCAC CGACCCGAGC AAACCCTTCT CTATCAGATC GTTGACGAGT ATTACCCGGC ATTCGCTGCG CTTATGGCAG AGCAGGGAAA GGAATTGCCG GGCTATGTGC AACGGGAATT TGAAGAATTT CTCCAATGCG GGCGGCTGGA GCATGGCTTT CTACGGGTTC GCTGCGAGTC TTGCCACGCC GAGCACCTGG TCGCTTTCAG CTGTAATCCG GGCAGCGCAA CGGAACATTC ATCAGTGTAA AAATGGAATC AATAAAGCCC TGCGCAGCGC GCAGGGTCAG CCTGAATACG CGTTTAATGA CCAGCACAGT CGTGATGGCA AGGTCAGAAT AGCGCTGAGG TCTGCCTCGT GAAGAAGGTG TTGCTGACTC ATACCAGGCC TGAATCGCCC CATCATCCAG CCAGAAAGTG AGGGAGCCAC GGTTGATGAG AGCTTTGTTG TAGGTGGACC AGTTGGTGAT TTTGAACTTT TGCTTTGCCA CGGAACGGTC TGCGTTGTCG GGAAGATGCG TGATCTGATC CTTCAACTCA GCAAAAGTTC GATTTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA ATGCTCTGCC AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGCTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAGCACC CCTTGTATTA CTGTTTATGT AAGCAGA
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