pECMV-Erbb3-m-FLAG
$84.00
In stock
SKU
PL79070
100μg liquid plasmid(Instantly):$105
| name | pECMV-Erbb3-m-FLAG | another name | apECMV-Erbb3-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGTGCGATT GGGACTCTGC AGGTGCTGGG TTTCCTTCTC AGCCTGGCCC GGGGTTCCGA GATGGGCAAC TCTCAGGCAG TATGTCCTGG GACTCTAAAC GGGCTGAGTG TGACCGGCGA TGCTGACAAC CAGTACCAGA CACTGTACAA ACTCTATGAG AAGTGTGAGG TGGTCATGGG TAACCTGGAG ATTGTGCTTA CGGGACACAA TGCTGATCTT TCCTTCCTGC AATGGATCCG AGAAGTGACA GGCTATGTAC TGGTGGCCAT GAATGAATTC TCTGTACTGC CCTTACCTAA CCTCCGAGTG GTCCGGGGAA CCCAGGTCTA CGATGGGAAG TTTGCCATCT TTGTCATGTT GAACTACAAT ACCAACTCCA GCCATGCTCT GCGCCAGCTC CGGTTCACTC AGCTTACTGA GATTCTGTTA GGGGGCGTTT ACATTGAGAA GAATGACAAA CTTTGCCACA TGGATACAAT TGACTGGAGG GACATCGTGA GGGTTCCAGA CGCTGAGATA GTGGTGAAGA ACAACGGGGG GAACTGTCCA CCCTGTCACG AAGTCTGCAA GGGGCGATGC TGGGGGCCTG GACCAGAAGA CTGCCAGATA TTGACCAAGA CCATCTGTGC CCCTCAGTGT AACGGTCGCT GCTTCGGGCC CAATCCTAAC CAGTGCTGCC ACGATGAATG TGCAGGTGGC TGCTCTGGAC CCCAGGACAC AGATTGCTTC GCCTGCCGAC ACTTCAATGA CAGTGGTGCC TGTGTGCCCA GGTGTCCAGC GCCCCTTGTG TACAACAAGC TAACGTTCCA GCTTGAGCCC AACCCCCATA TCAAGTATCA GTACGGAGGA GTCTGTGTTG CCAGTTGTCC CCATAACTTT GTGGTGGATC AGACATTTTG TGTCAGGGCT TGTCCTGCTG ACAAGATGGA AGTAGATAAG AATGGACTCA AGATGTGTGA GCCTTGCAGA GGGCTGTGCC CAAAAGCCTG TGAGGGGACG GGCTCTGGAA GCCGCTACCA GACCGTGGAC TCTAGCAATA TCGATGGGTT CGTGAACTGT ACCAAGATCC TGGGCAACCT GGACTTCCTC ATCACTGGCC TCAATGGTGA CCCCTGGCAC AAGATCCCTG CACTGGACCC GGAAAAGCTC AATGTTTTCA GGACAGTCCG GGAGATTACA GGCTACCTAA ACATCCAGTC CTGGCCCCCT CACATGCACA ACTTCAGTGT TTTTTCCAAC CTGACGACCA TCGGGGGCAG AAGCCTCTAC AATCGGGGCT TCTCCTTGTT GATCATGAAG AACTTGAATG TCACGTCTCT GGGCTTCCGG TCCCTGAAGG AAATTAGTGC TGGGCGTGTC TACATAAGTG CCAATCAGCA ACTTTGTTAC CACCACTCTC TGAACTGGAC CAGACTTCTG CGGGGGCCCG CAGAGGAGAG ACTTGACATC AAGTACAACC GGCCTCTGGG AGAATGCGTG GCAGAGGGCA AAGTGTGTGA TCCACTGTGC TCCTCTGGGG GATGCTGGGG CCCAGGCCCT GGTCAGTGCT TGTCTTGTCG AAACTACAGC CGGGAAGGTG TCTGTGTGAC TCACTGCAAC GTTCTGCAAG GGGAACCCCG AGAGTTTGTT CATGAGGCTC ATTGCTTCTC CTGCCATCCA GAATGCCAGC CCATGGAGGG CACCAGCACG TGCAATGGCT CGGGCTCCGA CGCTTGTGCT CGATGCGCCC ATTTTCGTGA TGGGCCCCAC TGTGTGAACA GCTGCCCCCA TGGAATCCTA GGTGCCAAAG GTCCAATCTA CAAATATCCA GATGCTCAGA ATGAGTGCCG GCCCTGCCAC GAGAACTGCA CCCAAGGGTG TAAGGGACCA GAACTACAAG ACTGTTTAGG CCAAGCAGAG GTATTAATGA GCAAACCACA CCTGGTCATA GCGGTGACAG TAGGACTGAC TGTGATCTTC CTGATTCTGG GAGGCTCTTT TCTCTATTGG CGTGGACGCA GGATTCAGAA TAAAAGGGCT ATGAGACGCT ACTTGGAGCG GGGTGAGAGC ATCGAGCCTC TGGACCCAAG CGAGAAGGCA AACAAAGTCT TGGCTAGAAT CTTCAAAGAG ACAGAGCTGA GGAAACTTAA GGTGCTTGGC TCTGGTGTCT TTGGAACTGT ACACAAGGGG ATTTGGATTC CCGAGGGTGA ATCCATCAAG ATTCCAGTCT GCATTAAAGT CATCGAGGAC AAGAGTGGGC GGCAGAGTTT TCAGGCTGTG ACTGATCACA TGCTGGCCGT CGGCAGCCTG GACCATGCCC ACATTGTACG GCTGCTGGGA CTGTGCCCAG GGTCATCTCT GCAGCTTGTC ACTCAGTACT TGCCTCTGGG CTCTCTCCTT GATCATGTAA GACAGCACCG TGAGACACTG GGACCACAGC TGCTGCTCAA CTGGGGAGTA CAAATTGCCA AGGGTATGTA TTACCTCGAG GAACACAGCA TGGTGCATAG GGACCTTGCG CTCCGGAATG TGATGCTTAA GTCACCGAGT CAAGTCCAGG TGGCAGATTT TGGTGTGGCT GACTTGCTGC CGCCAGATGA CAAGCAGTTA CTACACAGTG AGGCCAAGAC TCCAATTAAA TGGATGGCCC TTGAGAGTAT CCACTTTGGG AAATACACAC ACCAGAGTGA TGTCTGGAGT TACGGTGTAA CCGTTTGGGA GTTGATGACC TTCGGGGCAG AGCCCTACGC AGGGCTACGA CTGGCTGAAA TACCAGACCT GCTGGAGAAG GGAGAGCGGT TAGCACAGCC CCAGATCTGC ACCATTGACG TCTACATGGT CATGGTCAAG TGTTGGATGA TTGACGAGAA TATTCGCCCA ACCTTTAAAG AACTGGCCAA TGAGTTTACC AGGATGGCCC GGGACCCACC AAGGTATCTG GTCATCAAGA GAGCGAGTGG GCCTGGAATA CCTCCTGCAG CAGAGCCATC TGCTCTGAGC ACCAAAGAGT TGCAGGATGC AGAGCTGGAG CCAGACCTGG ACCTCGACCT AGACGTGGAG GTAGAAGAGG AGGGCCTGGC GACCACACTG GGTTCTGCCC TCAGCTTGCC TACAGGAACG CTTACCCGGC CACGTGGGAG CCAGAGTCTT TTAAGTCCTT CGTCTGGATA CATGCCCATG AACCAGAGCA ACCTTGGGGA GGCTTGTCTG GATTCTGCGG TTTTGGGGGG TCGCGAACAG TTCTCCCGTC CCATCTCTCT GCACCCGATC CCACGGGGGC GTCAAACGTC AGAGTCATCA GAGGGCCATG TGACGGGCTC TGAGGCTGAA CTCCAAGAGA GAGTATCAAT GTGTAGGAGC CGGAGCCGGA GCCGGAGCCC ACGGCCACGT GGGGACAGTG CCTACCATTC GCAGCGACAC AGCCTGCTTA CTCCCGTCAC CCCGCTCTCC CCACCAGGGT TAGAGGAAGA GGATGGCAAT GGTTATGTCA TGCCAGATAC GCACCTCAGA GGTACATCCT CTTCCCGGGA AGGCACCCTT TCGTCAGTAG GTCTCAGTTC TGTGCTGGGT ACCGAAGAGG AAGATGAAGA TGAGGAGTAT GAATACATGA ACCGGAAGAG GAGGGGTAGC CCGGCTCGGC CCCCCAGACC TGGTTCCCTG GAAGAGCTGG GCTATGAGTA CATGGATGTG GGTTCAGACC TCAGTGCTTC TCTGGGCAGT ACGCAGAGTT GCCCACTCCA TCCCATGGCC ATCGTGCCCT CTGCTGGCAC GACTCCAGAT GAGGACTATG AATACATGAA CCGCAGGCGT GGTGCGGGCG GTTCCGGAGG GGATTATGCA GCTATGGGGG CCTGCCCAGC AGCTGAACAA GGGTATGAGG AAATGCGAGC TTTCCAGGGG CCTGGACATC AAGCCCCCCA TGTTCGTTAT GCCCGCCTCA AAACTCTGCG TAGTTTAGAA GCCACTGACT CCGCCTTTGA CAACCCCGAT TACTGGCATA GCAGGCTTTT CCCTAAGGCT AACGCCCAGA GAATTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
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