pCMV-Gli1(mouse)-Myc-FLAG-Neo
$84.00
In stock
SKU
PL79047
Zfp-5; Zfp5
100μg liquid plasmid(Instantly):$105
| name | pCMV-Gli1(mouse)-Myc-FLAG-Neo | another name | apCMV-Gli1(mouse)-Myc-FLAG-Neo |
| Insert Size (bp) | 3333 | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTCAATCCA ATGACTCCAC CACAAGTCAA TAGCTATAGT GAGCCATGCT GTCTCCGACC CCTCCACAGC CAAGGAGTCC CCAGCATGGG AACAGAAGGA CTTTCTGGTC TGCCCTTTTG CCACCAAGCC AACTTTATGT CAGGGTCCCA GGGTTATGGA GCAGCCAGAG AGACCAGCAG CTGCACTGAA GGATCTCTCT TTCCTCCTCC TCCTCCTCCT CGGAGTTCAG TCAAATTAAC AAAGAAGCGG GCTCTCTCCA TCTCGCCCCT TTCTGATGCC AGCCTCGACC TGCAAACCGT AATCCGGACC TCACCCAGCT CCCTGGTGGC TTTCATCAAC TCTCGCTGTA CATCTCCGGG CGGTTCCTAC GGCCATCTCT CCATTGGTAC CATGAGCCCT TCTTTAGGAT TCCCACCTCA GATGAGTCAT CAAAAAGGAA CTTCACCTCC CTATGGAGTC CAGCCCTGTG TACCACATGA CTCTACTCGG GGTTCAATGA TGCTTCACCC CCAGTCCCGG GGACCACGTG CAACCTGCCA GCTGAAGTCA GAGCTGGATA TGATGGTTGG CAAGTGCCCG GAGGACCCTT TGGAAGGGGA CATGTCTAGC CCCAACTCCA CAGGCACACA GGATCACCTG TTGGGGATGC TGGATGGGCG GGAGGACCTG GAGAGAGAGG AGAAGCCTGA GCCTGAGTCT GTGTATGAGA CAGACTGCCG CTGGGATGGT TGCAGCCAGG AGTTCGATTC CCAGGAGCAG CTGGTGCACC ACATCAACAG TGAGCATATC CACGGGGAGC GGAAGGAATT CGTGTGCCAT TGGGGAGGTT GCTCCAGGGA GCTGAGGCCC TTCAAGGCCC AATACATGCT GGTGGTGCAC ATGCGCAGAC ACACGGGCGA GAAGCCACAC AAGTGCACGT TTGAAGGCTG TCGGAAGTCC TATTCACGCC TTGAAAACCT CAAGACGCAC CTTCGGTCGC ACACGGGTGA GAAGCCTTAC ATGTGTGAGC AAGAAGGTTG CAGCAAGGCC TTTAGCAATG CCAGTGACCG CGCCAAGCAC CAGAATCGGA CCCACTCCAA TGAGAAGCCA TACGTGTGCA AGCTCCCCGG CTGCACCAAG CGCTACACAG ATCCCAGCTC GCTCCGCAAA CACGTGAAGA CAGTGCATGG TCCGGATGCC CACGTGACCA AGCGGCATCG AGGGGATGGC CCCTTGCCAC GGGCTCAGCC CCTCTCCACA GTGGAGCCCA AGCGGGAAAG GGAAGGAGGA TCCGGCAGGG AAGAGAGCAG ACTGACTGTG CCCGAGAGTG CCATGCCGCA GCAGAGCCCC GGAGCGCAGT CCTCTTGCAG CAGCGACCAC TCCCCAGCAG GCAGTGCGGC CAACACGGAC AGCGGCGTGG AGATGGCCGG CAACGCCGGG GGCAGCACTG AGGACTTGTC CAGCTTGGAT GAAGGACCTT GTGTCTCGGC CACCGGACTC TCCACGCTTC GCCGCCTGGA GAACCTTAGG CTGGATCAGC TGCATCAGCT CCGGCCCATA GGGTCTCGGG GTCTCAAACT GCCCAGCTTA ACCCACGCTG GCGCACCTGT GTCTCGCCGT CTGGGCCCCC CAGTCTCCCT GGACCGCCGC AGCAGCAGCT CCAGCAGCAT GAGCTCTGCT TACACAGTCA GCCGCAGGTC CTCCCTGGCA TCCCCTTTCC CGCCGGGAAC CCCACCAGAG AATGGGGCAT CGTCACTACC TGGCCTCACA CCTGCTCAGC ACTACATGCT CCGTGCCAGA TATGCTTCAG CCAGGGGGAG TGGCACCCCG CCCACTGCAG CTCACAGCCT GGATCGGATG GGAGGTCTTT CTGTTCCTCC TTGGAGAAGC CGAACCGAGT ACCCGGGATA CAACCCAAAT GCAGGGGTCA CTCGGAGGGC CAGTGACCCA GCCCGGGCTG CTGACCACCC AGCTCCAGCC AGAGTCCAGC GGTTCAAGAG CCTGGGATGT GTCCACACGC CCCCTAGTGT GGCAACGGGA CGGAACTTCG ATCCCCACCA CCCTACCTCT GTCTATTCGC CACAGCCCCC CAGCATCACC GAAAATGTTG CCATGGATAC TAGGGGGCTA CAGGAGGAGC CAGAGGTTGG AACTTCTGTG ATGGGCAATG GTCTGAACCC ATACATGGAT TTTTCCTCCA CTGATACTCT GGGATATGGG GGACCCGAGG GGACGGCAGC TGAGCCTTAT GAAGCTAGGG GTCCAGGTTC CCTGCCTCTT GGGCCTGGTC CACCAACCAA CTATGGCCCT GGCCACTGTG CCCAGCAGGT CTCCTATCCT GATCCCACCC CAGAAAACTG GGGTGAGTTC CCTTCTCATG CTGGGGTGTA CCCTAGCAAT AAGGCTCCGG GTGCTGCCTA TAGCCAGTGT CCTCGACTTG AGCATTATGG ACAAGTGCAG GTAAAACCAG AACAAGGGTG CCCAGTGGGG TCTGACTCCA CCGGATTGGC ACCCTGCCTC AATGCCCACC CCAGTGAAGG GTCCCCAGGC CCGCAGCCTC TGTTTTCACA TCATCCCCAG CTCCCTCAGC CCCAGTATCC CCAGTCGGGT CCCTATCCTC AGCCTCCCCA TGGTTATCTC TCAACAGAAC CCAGGCTTGG CCTCAATTTC AACCCCTCCT CCTCTCATTC CACAGGACAG CTCAAAGCTC AGCTGGTGTG TAATTACGTT CAGTCGCAGC AGGAATTGTT GTGGGAGGGA AGAAACCGGG GAGGGCTCCC CAACCAGGAA CTCCCATACC AGAGCCCCAA GTTTCTGGGG GGTTCCCAAG TTAGTCAGAG CCCTGCCAAG ACCCCAGCAG CAGCGGCGGC AGCATATGGA TCTGGCTTTG CACCTGCTTC GGCCAATCAC AAATCAGGCT CCTATCCTGC CCCTTCACCC TGCCATGAAA CTTTCACCGT GGGAGTAAAC AGGCCTTCCC ACAGGCCAGC AGCACCACCC CGACTTCTGC CCCCGCTGTC CCCTTGCTAT GGGCCCCTCA AGGTGGGGGA TACCAACCCC AGCTGTGGCC ATCCTGAGGT GGGCAGGTTA GGAGCAGGCC CTGCCTTGTA CCCTCCTCCT GAAGGGCAGG TGTGTAACGC TCTGGACTCT CTTGACCTGG ACAACACTCA GCTGGACTTT GTGGCTATCC TAGATGAGGC CCAGGGCCTG AGCCCTCCTC TTTCCCATGA GCAAGGGGAC AGCTCTAAAA ACACCCCATC TCCCTCTGGG CCCCCCAACA TGGCAGTGGG TAACATGAGT GTCTTGCTGG GGTCTCTGCC TGGAGAGACA CAATTCCTCA ACTCTAGTGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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