pECMV-Ptprm-m-FLAG
$84.00
In stock
SKU
PL79019
100μg liquid plasmid(Instantly):$105
| name | pECMV-Ptprm-m-FLAG | another name | apECMV-Ptprm-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGACACTT GGGACTTGCC TGGTGACTTT AGCTGGACTT TTGCTAACTG CGGCCGGAGA AACATTTTCA GGTGGCTGCC TCTTTGATGA ACCATACAGC ACATGTGGAT ATAGTCAAGC TGACGAAGAT GACTTCAACT GGGAGCAGGT GAACACCCTG ACCAAACCGA CCTCTGACCC CTGGATGCCA TCAGGCTCCT TCATGCTGGT CAACACTTCT GGGAAACCAG AAGGCCAGAG AGCTCACCTG CTCCTGCCTC AGCTTAAGGA AAATGACACC CACTGCATCG ACTTTCACTA CTTTGTATCC AGCAAGAGTA ACGCTGCTCC TGGGTTGCTC AATGTCTATG TGAAGGTGAA TAATGGACCA CTGGGGAATC CCATCTGGAA CATATCCGGA GACCCCACCC GCACGTGGCA CAGGGCAGAG CTGGCCATCA GCACGTTCTG GCCCAACTTT TACCAGGTGA TTTTTGAAGT GGTGACCTCT GGCCATCAAG GCTACCTTGC TATCGATGAA GTTAAGGTCT TAGGACACCC GTGCACAAGA ACTCCCCACT TCCTGCGGAT TCAGAACGTG GAGGTGAATG CCGGCCAGTT TGCTACCTTC CAGTGCAGCG CCATAGGCAG GACAGTGGCT GGGGACCGGC TCTGGCTGCA GGGAATTGAT GTCCGAGATG CCCCTCTGAA GGAGATAAAG GTGACTAGTT CCAGAAGATT CATAGCCTCA TTTAACGTTG TAAACACGAC CAAGAGAGAC GCTGGGAAGT ACCGCTGCAT GATTTGCACG GAAGGAGGTG TCGGGATATC AAACTACGCA GAATTGGTAG TTAAAGAGCC ACCTGTTCCC ATCGCCCCAC CTCAGCTCGC CTCTGTCGGA GCAACCTATC TGTGGATACA GCTCAATGCC AACTCCATCA ATGGGGACGG ACCCATTGTG GCCCGCGAGG TTGAGTACTG CACAGCCAGC GGGAGCTGGA ATGATCGGCA GCCAGTGGAT TCCACGAGTT ACAAGATTGG GCACCTTGAC CCAGACACAG AGTATGAGAT CAGTGTGCTT CTTACCAGAC CAGGAGAAGG CGGCACTGGG TCTCCCGGGC CAGCTCTGAG AACAAGGACA AAGTGTGCCG ATCCCATGCG TGGCCCGAGA AAACTAGAAG TTGTGGAGGT CAAATCTCGA CAAATCACCA TCCGCTGGGA GCCGTTTGGA TACAATGTAA CTCGTTGCCA CAGTTATAAT CTCACAGTGC ACTATGGTTA CCAGGTCGGA GGGCAGGAGC AGGTGCGAGA AGAAGTGAGC TGGGATACAG ATAACTCCCA CCCTCAGCAC ACCATCACCA ACCTGTCCCC GTACACCAAC GTCAGCGTGA AGCTTATTCT GATGAACCCG GAGGGCCGCA AGGAAAGCCA AGAACTCACG GTGCAGACGG ATGAAGACCT CCCAGGAGCT GTCCCCACTG AATCCATCCA AGGCAGTGCC TTTGAAGAGA AGATATTTCT TCAGTGGAGA GAGCCGACAC AAACATATGG GGTCATCACT TTATATGAGA TCACCTACAA AGCAGTCAGT TCCTTTGACC CGGAAATAGA TTTATCCAAC CAAAGTGGGC GAGTTTCAAA ACTGGGAAAT GAAACCCATT TTCTGTTCTT TGGCCTGTAT CCTGGAACGA CGTACTCCTT CACCATCAGA GCCAGCACAG CGAAGGGGTT CGGACCTCCG GCAACAAACC AGTTCACCAC AAAAATATCA GCACCATCCA TGCCAGCCTA TGAATTTGAG ACACCTTTGA ATCAGACTGA TAATACAGTG ACTGTGATGC TGAAACCTGC ACAAAGCAGA GGGGCTCCAG TCAGTGTCTA TCAAATCGTT GTTGAGGAAG AACGTCCTCG GAGAACTAAG AAGACAACAG AGATCTTGAA GTGTTACCCG GTGCCAATTC ACTTCCAGAA TGCGTCCATC CTGAACTCAC AGTACTACTT TGCTGCAGAA TTTCCTGCAG ACAGCCTCCA AGCTGCTCAA CCATTTACCA TTGGTGACAA TAAGACATAC AATGGCTACT GGAACACCCC CCTCCTCCCC CATAAAAGCT ACAGAATTTA TTACCAAGCT GCTAGTCGAG CCAATGGGGA AACCAAAATT GACTGTGTCC GAGTGGCCAC AAAAGGGGCC GTTACTCCAA AGCCTGTCCC TGAACCTGAG AAACAAACAG ACCACACGGT TAAAATCGCC GGAGTGATTG CAGGCATCTT GCTGTTTGTT ATCATATTTC TTGGAGTCGT GCTGGTCATG AAGAAGAGGA AACTGGCCAA GAAGAGGAAG GAGACGATGA GCAGCACCCG GCAGGAGATG ACAGTGATGG TGAATTCTAT GGATAAGAGC TATGCTGAGC AAGGCACCAA CTGCGACGAA GCCTTCTCTT TCATGGGCAC GCACAATCTT AATGGGAGAT CTGTGTCCTC GCCATCATCC TTTACCATGA AAACAAACAC GCTGAGCACA TCGGTGCCGA ATTCCTATTA CCCAGATGAG ACCCACACAA TGGCCAGTGA CACCAGCAGC CTGGCACAGC CCCATACTTA TAAGAAACGC GAGGCCGCAG ACGTGCCTTA CCAGACTGGG CAGCTTCACC CGGCCATCCG TGTGGCAGAC CTCCTCCAAC ACATCACTCA GATGAAGTGC GCTGAGGGCT ATGGCTTCAA GGAGGAGTAT GAGAGCTTCT TTGAAGGACA GTCTGCACCA TGGGACTCTG CTAAGAAAGA CGAGAACAGA ATGAAGAACA GATACGGGAA CATCATTGCA TATGATCACT CTCGGGTACG GCTCCAGATG CTGGAAGGGG ACAACAATTC AGACTACATC AATGGCAATT ACATCGATGG CTATCATCGA CCCAATCATT ATATTGCAAC CCAAGGACCC ATGCAGGAGA CTATCTATGA CTTCTGGAGG ATGGTGTGGC ATGAGAACAC GGCAAGCATC ATCATGGTGA CCAACCTTGT GGAAGTGGGA AGGGTGAAAT GCTGTAAATA CTGGCCAGAT GACACCGAGA TCTACAAAGA CATTAAAGTC ACCCTAATAG ATACAGAGCT GCTGGCAGAA TATGTGATAA GAACGTTTGC CGTGGAAAAG AGAGGCATTC ATGAAATCCG AGAGATCAGA CAGTTTCACT TCACCGGCTG GCCGGACCAC GGCGTCCCCT ACCATGCCAC TGGCCTGCTG GGATTTGTCC GGCAGGTCAA ATCCAAGAGC CCACCCAATG CAGGCCCGCT GGTGGTACAC TGCAGTGCTG GAGCAGGAAG GACAGGTTGC TTCATTGTCA TTGATATCAT GCTGGACATG GCAGAAAGGG AAGGAGTGGT GGACATCTAC AACTGTGTCA GAGAGCTTCG GTCCAGGAGA GTCAACATGG TGCAGACAGA GGAGCAGTAC GTCTTTATCC ATGATGCGAT CCTGGAAGCC TGTCTTTGTG GAGACACCTC CATCCCTGCC TCCCAAGTCA GGTCTCTCTA CTATGACATG AACAAACTGG ACCCACAGAC AAACTCAAGC CAAATTAAAG AGGAATTCCG GACTCTCAAC ATGGTGACCC CCACGCTGCG TGTGGAGGAC TGCAGCATCG CACTCCTACC CCGGAACCAC GAGAAAAATC GCTGCATGGA CATCCTGCCT CCGGACCGCT GCCTGCCCTT CCTCATCACC ATCGATGGAG AGAGCAGCAA CTACATCAAT GCAGCCCTAA TGGACAGCTA TAAACAGCCT TCAGCATTTA TAGTCACCCA GCATCCTTTG CCAAACACCG TCAAAGACTT CTGGCGACTG GTCTTGGATT ACCACTGCAC ATCCGTAGTT ATGCTGAACG ATGTGGATCC TGCCCAGCTG TGTCCACAGT ACTGGCCAGA AAATGGAGTG CACAGACATG GCCCCATCCA GGTGGAATTC GTGTCTGCAG ACTTAGAAGA AGACATCATC AGCAGAATTT TCAGGATTTA CAACGCCTCC AGACCCCAGG ATGGACATCG GATGGTTCAG CAGTTCCAGT TCCTGGGCTG GCCGATGTAC AGGGACACGC CCGTGTCCAA GCGCTCCTTC TTGAAGCTCA TACGCCAGGT GGACAAGTGG CAGGAGGAAT ACAATGGCGG CGAAGGCCGC ACAGTCGTGC ACTGCTTGAA CGGAGGAGGC CGCAGTGGTA CATTCTGTGC CATCAGCATT GTCTGTGAGA TGCTCCGACA CCAGAGAACT GTGGATGTCT TCCATGCTGT CAAGACATTG AGGAACAACA AACCAAACAT GGTGGACCTC CTGGATCAGT ACAAGTTCTG CTATGAGGTG GCCCTGGAGT ACCTGAATTC GGGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
