pECMV-Atm-m-FLAG
$171.00
In stock
SKU
PL78796
NM_007499.2
100μg liquid plasmid(Instantly):$192
| name | pECMV-Atm-m-FLAG | another name | apECMV-Atm-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGTCTAGCA CTCAATGATC TGCTCATTTG CTGCCGGCAG TTAGAGCATG ACAGAGCTAC AGAAAGAAGG AAAGAAGTGG ATAAATTTAA GCGCCTGATT CAGGATCCTG AAACAGTTCA ACATTTAGAT AGGCATTCTG ATTCCAAACA AGGAAAATAT CTGAATTGGG ATGCTGTTTT CAGGTTTTTA CAGAAGTACA TTCAAAAAGA AATGGAAAGT CTGAGAACAG CAAAATCAAA TGTATCAGCC ACCACACAGA GCTCCAGACA GAAGAAGATG CAAGAGATCA GCAGTTTGGT CAGATACTTC ATCAAATGTG CAAACAAAAG AGCACCCAGG CTAAAATGTC AAGACCTCTT GAATTATGTC ATGGATACAG TGAAAGACTC ATCTAATGGT CTAACGTATG GAGCTGACTG TAGCAACATA CTACTCAAAG ACATTCTTTC TGTGAGAAAA TACTGGTGTG AAGTATCTCA GCAACAGTGG CTAGAATTGT TTTCACTGTA CTTCAGGCTG TATCTCAAGC CATCACAGGA CATTAATAGA GTTTTAGTGG CTAGAATAAT TCATGCTGTC ACCAGAGGAT GCTGTTCACA GACTGATGGA TTACCTTCAA AGTTTTTAGA TCTTTTTTCC AAGGCTATTC AGTATGCCAG ACAAGAAAAG AGCTCTCCTG GCTTAAGTCA CATCTTAGCA GCCCTTAACA TTTTCCTCAA GTCTCTGGCT GTCAACTTCC GAAAACGGGT GTGTGAAGCA GGAGATGAAA TTCTTCCTAC CTTACTATAT ATTTGGACTC AACATAGACT TAATGATTCT TTAAAAGAAG TAATTATTGA ACTAATTCAA CTGCAGATTT ATATCCATCA TCCACAAGGA GCCAGAGCTC CTGAAGAAGG TGCTTATGAA TCCATGAAAT GGAAAAGTAT CTTGTACAAC TTATATGACT TGCTAGTGAA TGAGATAAGT CATATAGGAA GCCGAGGGAA ATATTCCTCA GGATCTCGTA ATATTGCTGT CAAGGAAAAT CTGATTGACC TGATGGCAGA TATCTGTTAC CAGCTTTTTG ATGCAGATAC CAGATCCGTG GAGATTTCTC AATCTTATGT GACACAAAGG GAATCCACTG ATTACAGTGT ACCTTGCAAA AGAAGGAAAA TAGACGTAGG CTGGGAAGTG ATAAAAGATT ATCTTCAGAA GTCACAGAGT GATTTTGATC TCGTGCCTTG GCTACAGATT ACAACCCGAT TAATATCAAA ATATCCTTCC AGTTTACCTA ACTGTGAGCT GTCTCCATTA ATACTGATAC TGTACCAGCT TCTGCCTCAA CAGCGACGTG GAGAACGCAT CCCATATGTG TTACGATGCC TTAAGGAAGT TGCCTTATGT CAAGGCAAGA AATCAAACCT GGAAAGCTCT CAGAAGTCAG ATTTATTGAA ACTATGGATC AAAATTTGGT CTATTACCTT TCGTGGTATA AGTTCTGGAC AAACACAAAC TGAAAACTTT GGTTTACTTG AGGCCATCAT TCAAGGTAGT TTAGTTGAAC TTGACAGAGA ATTCTGGAAG TTATTTACTG GCTCAGCCTG TAAACCTTCT AGTCCTTCAG TATGCTGCTT GACTTTGGCA CTTAGCATCT GTGTAGTTCC AGATGCAATA AAAATGGGAA CAGAACAAAG TGTGTGTGAA GCAAATAGAA GTTTTTCTGT AAAGGAGTCA ATAATGAGGT GGCTCTTATT CTACCAGTTA GAGGATGACT TAGAAGACAG CACAGAGCTG CCTCCAATTC TTCAGAGTAA TTTTCCTCAT CTTGTAGTCG AAAAAATTCT TGTAAGTCTC ACTATGAAAA ACTCAAAAGC TGCAATGAAG TTTTTTCAAA GTGTGCCAGA ATGTGAACAA CACTGCGAAG ATAAAGAAGA GCCTTCATTT TCAGAAGTAG AAGAACTGTT TCTTCAGACT ACTTTTGACA AGATGGATTT TTTAACTACT GTCAAAGAGT ATGCTGTAGA AAAATTTCAG TCTAGTGTTG GCTTCTCTGT CCAGCAAAAT CTCAAGGAAT CATTGGATCA CTATCTTCTG GGATTATCAG AACAGCTTTT AAGTAATTAC TCTTCTGAGA TTACAAGTTC TGAAACCCTT GTCCGGTGTT CAAGTCTTTT GGTGGGTGTT CTTGGCTGCT ATTGTTACAT GGGTATAATA ACTGAAGACG AAGCCCATAA ATCAGAATTA TTCCAGAAAG CCAAGTCTCT GATGCAATGT GCAGGAGAAA GTATCTCTCT GTTTAAAAAT AAAACAAATG AGGAATCAAG AATTGGTTCA TTGAGAAATG TGATGCATCT GTGTACAAGT TGCTTGTGTA TACATACCAA GCATACGCCA AACAAGATTG CCTCTGGCTT TTTCCTACGA TTATTAACAT CAAAGCTTAT GAATGACATT GCAGATATTT GTAAAAGTTT AGCATCCTGT ACGAAAAAGC CATTGGATCA CGGAGTACAT CCAGGGGAAG ATGATGAAGA TGGTGGTGGT TGTGACAGTC TGATGGAGGC AGAGGGTCCA TCGTCCACTG GTCTTTCTAC TGCTTACCCC GCTAGTTCTG TGAGCGATGC AAATGATTAT GGAGAGAACC AGAATGCTGT TGGTGCCATG AGTCCTTTAG CTGCCGACTA CCTGTCCAAA CAAGATCATC TTCTCTTAGA CATGCTCAGG TTCTTAGGCC GATCTGTAAC TGCATCTCAG AGCCATACTG TGTCGTTTAG AGGAGCTGAC ATTAGAAGAA AATTGTTACT GTTGCTTGAT TCTAGCATAC TCGATCTCAT GAAGCCCCTC CACCTGCATA TGTACTTAGT GCTCCTGAAG GATCTCCCTG GAAACGAGCA CTCATTGCCA ATGGAAGATG TTGTTGAACT TCTGCAACCA TTATCCCTTG TGTGTTCTCT GCACCGACGT GACCAAGATG TCTGTAAAAC GATTCTAAGC AATGTCCTTC ATATAGTGAC AAACCTAGGC CAGGGCAGTG TGGACATGGA GAGCACACGG ATTGCTCAAG GACACTTCCT GACAGTGATG GGAGCATTTT GGCATTTGAC AAAGGAAAAG AAATGTGTAT TCTCTGTAAG AATGGCATTA GTAAAGTGTC TTCAAACATT GCTTGAGGCT GATCCATATT CCGAATGGGC AATTCTTAAT GTAAAAGGAC AAGACTTTCC TGTAAATGAA GCTTTTTCAC AATTTCTTGC TGACGATCAT CATCAAGTTC GGATGTTGGC TGCAGGGTCA GTCAACAGAT TATTTCAGGA TATGAGACAA GGCGATTTCT CCAGAAGCTT GAAAGCACTC CCTCTGAAGT TTCAGCAGAC ATCTTTTAAC AATGCATACA CGACAGCAGA GGCGGGGATC AGAGGACTGT TATGTGATTC TCAGAACCCT GATCTGCTGG ATGAGATCTA TAACAGAAAA TCTGTACTAC TGATGATGAT AGCTGTGGTC TTGCACTGTA GCCCAGTCTG TGAAAAGCAG GCTTTGTTTG CTTTATGCAA GTCTGTGAAG GAAAACAGAC TAGAACCTCA TCTTGTGAAA AAGGTTTTAG AGAAAGTCTC CGAATCGTTT GGATGTAGAA GTTTAGAAGA CTTCATGATT TCTCACCTAG ACTACCTGGT TTTGGAATGG CTGAACCTTC AAGATACTGA ATATAGCTTA TCTTCTTTTC CTTTTATGTT ATTAAACTAC ACAAGCATTG AGGATTTCTA TCGGTCTTGT TACAAGATTT TGATCCCACA TTTGGTAATC AGAAGCCATT TTGATGAGGT GAAGTCCATT GCTAATCAGA TTCAAAAGTG CTGGAAAAGC CTGTTGGTAG ATTGCTTTCC GAAGATTCTT GTGCACATCC TTCCTTACTT TGCCTACGAG GGCACGAGAG ACAGCTACGT GTCACAGAAA AGAGAGACTG CTACCAAGGT CTACGATACT CTTAAAGGGG AAGACTTCCT AGGAAAACAG ATTGACCAAG TATTCATTAG TAATTTGCCA GAGATTGTGG TGGAGTTGCT GATGACATTG CATGAGACAG CTGACTCGGC TGACTCGGAC GCCAGTCAAA GCGCCACCGC CTTGTGTGAT TTTTCAGGGG ATTTGGATCC TGCCCCCAAC CCGCCATATT TCCCCTCACA TGTCATTCAG GCAACGTTTG CTTACATCAG CAACTGTCAT AAAACCAAGT TTAAAAGCAT TCTAGAAATT CTTTCTAAAA TCCCCGATTC CTATCAGAAA ATACTTCTGG CCATTTGTGA ACAAGCAGCT GAGACAAATA ATGTCTTTAA AAAGCACAGA ATTCTTAAAA TATATCACCT GTTTGTTAGT TTATTACTGA AAGATATACA GAGTGGCCTG GGAGGGGCTT GGGCCTTTGT CCTTCGCGAT GTTATTTATA CTCTGATTCA CTACATCAAC AAAAGGTCTT CTCATTTCAC AGATGTGTCG TTGCGTAGCT TTTCCCTTTG CTGTGACCTA TTAAGTCGAG TTTGTCATAC AGCTGTAACT CAATGTAAGG ATGCTCTAGA AAGCCATCTT CACGTTATCG TTGGCACACT TATTCCCCTT GTGGATTATC AGGAAGTTCA AGAACAGGTA TTGGACCTGT TGAAGTACTT AGTGATAGAT AACAAAGACA ATAAAAACCT CTCTGTCACA ATTAAGCTTT TGGATCCCTT TCCTGACCAT GTTATTTTTA AGGACTTGCG TCTTACTCAA CAGAAAATCA AATATAGTGG AGGACCTTTT TCACTCTTAG AGGAAATAAA CCATTTTCTC TCAGTAAGTG CTTACAATCC ACTTCCGCTG ACCAGGCTTG AAGGACTGAA GGATCTTCGA AGACAACTGG AGCAACATAA AGATCAGATG CTAGATCTTC TGAGAGCGTC TCAAGATAAC CCACAAGATG GCATTGTGGT GAAGCTAGTT GTCAGCTTGT TGCAGTTATC CAAGATGGCA GTGAACCAGA CTGGTGAAAG AGAAGTTTTA GAGGCTGTCG GAAGGTGTTT GGGAGAAATA GGTCCTCTGG ATTTCTCCAC CATAGCTGTC CAGCATAACA AAGATGTGTC CTATACCAAA GCCTACGGGT TACCTGAAGA CAGAGAACTT CAGTGGACCT TGATAATGCT GACTGCCCTC AACAATACCC TGGTAGAGGA CAGTGTCAAA ATTCGATCTG CTGCTGCTAC CTGTTTGAAA AACATTTTGG CTACAAAGAT TGGACATATT TTCTGGGAGA ATTATAAGAC ATCAGCGGAT CCAATGCTGA CCTATCTACA ACCTTTTAGA ACATCGAGGA AAAAGTTTTT AGAAGTGCCC CGATCTGTTA AAGAAGATGT TTTAGAAGGC CTGGATGCTG TGAATCTGTG GGTTCCTCAA AGTGAAAGTC ATGACATTTG GATAAAGACA CTGACGTGTG CCTTTCTGGA CAGTGGAGGC ATAAACAGTG AAATTCTCCA GTTATTAAAG CCAATGTGTG AAGTGAAAAC CGACTTCTGT CAGATGTTGC TGCCATACTT GATCCATGAT GTTTTACTGC AAGATACACA TGAATCGTGG AGAACTCTGC TGTCTGCGCA CGTCCGAGGA TTTTTCACTA GTTGTTTTAA GCATTCCTCC CAAGCAAGCC GCTCAGCAAC TCCTGCAAAT TCGGATTCAG AGTCAGAGAA CTTTCTCCGA TGCTGTTTGG ATAAAAAGTC ACAAAGAACC ATGCTTGCTG TTGTCGACTA TCTGAGAAGG CAAAAGAGAC CTTCCTCGGG AACAGCTTTT GATGACGCTT TCTGGCTGGA TTTGAATTAT CTTGAGGTTG CGAAGGTGGC TCAGTCCTGC TCTGCTCACT TCACGGCCTT GCTCTACGCA GAGATCTATT CAGATAAGAA AAGCACAGAC GAGCAAGAGA AAAGAAGTCC AACATTTGAA GAAGGAAGTC AAGGAACAAC TATTTCTAGT TTGAGTGAAA AAAGTAAAGA AGAAACTGGA ATAAGCTTAC AGGATCTTCT CTTAGAGATC TACAGAAGTA TAGGAGAGCC GGACAGCCTG TATGGCTGTG GAGGAGGGAA AATGTTACAA CCCCTTACTA GAATACGGAC ATATGAACAT GAAGCTACGT GGGAGAAAGC CTTAGTAACT TACGACCTGG AGACCAGCAT CTCCTCCTCC ACCCGCCAGT CAGGAATCAT CCAGGCCCTG CAGAATTTGG GGCTCTCCCA TATCCTGTCT GTCTATCTGA AAGGATTAGA CTATGAAAGA CGAGAGTGGT GCGCTGAGCT GCAGGAGCTG CGTTACCAGG CGGCGTGGAG GAACATGCAG TGGGGCCTCT GCGCTTCTGC CGGCCAAGAA GTAGAAGGAA CCAGTTACCA TGAATCGTTG TATAATGCTC TGCAGTGTCT AAGAAACAGA GAATTCTCCA CATTTTATGA AAGTCTCCGA TATGCCAGTC TTTTCAGGGT GAAAGAAGTT GAAGAGTTGA GTAAGGGCAG CCTTGAGTCT GTATATTCGC TGTATCCCAC ACTTAGTAGA TTGCAGGCAA TTGGAGAACT GGAAAACAGT GGCGAGCTTT TCTCAAGGTC AGTCACAGAC AGAGAGCGCT CTGAAGCATA CTGGAAGTGG CAGAAGCACT CCCAGCTTCT GAAAGACAGC GACTTCAGCT TTCAGGAGCC TCTCATGGCT CTGCGCACAG TCATTCTGGA GACCCTGGTA CAGAAGGAAA TGGAGCGCTC TCAAGGAGCA TGCTCTAAGG ACATTCTCAC CAAACACCTC GTTGAATTCT CTGTTCTGGC TCGAACCTTC AAGAACACAC AGCTCCCTGA AAGAGCAATA TTCAAAATTA AGCAATATAA TTCAGCTATT TGTGGAATTT CTGAGTGGCA TTTGGAAGAA GCACAAGTAT TCTGGGCAAA AAAGGAGCAG AGTCTTGCTC TGAGTATTCT CAAGCAGATG ATCAAGAAGT TGGACTCCAG CTTTAAAGAT AAAGAGAATG ATGCAGGTCT CAAAGTCATA TACGCAGAGT GTCTGAGGGT TTGTGGCAGC TGGCTGGCAG AAACTTGCTT AGAAAACCCT GCAGTCATCA TGCAGACCTA TCTAGAAAAG GCGGTGAAGG TTGCTGGAAG TTACGATGGC AACAGCAGAG AGCTCAGAAA TGGACAGATG AAGGCCTTTC TCTCGTTGGC AAGGTTCTCT GATACTCAGT ACCAGAGAAT TGAAAACTAC ATGAAGTCAT CAGAATTTGA AAACAAGCAA ACTCTCTTAA AAAGAGCCAA AGAGGAAGTG GGCCTTCTAA GGGAACATAA AATTCAGACC AACAGATACA CAGTAAAGGT TCAGCGAGAA CTGGAGCTGG ACGAATGTGC TCTCCGTGCA CTGAGAGAGG ATCGCAAGCG CTTCCTGTGT AAAGCAGTGG AGAACTACAT CAACTGCTTA CTAAGCGGGG AAGAACATGA TCTGTGGGTG TTCCGGCTTT GCTCCCTCTG GCTTGAAAAT TCTGGAGTTT CTGAAGTCAA TGGCATGATG AAGAAAGATG GAATGAAGAT TTCATCCTAT AAGTTTTTGC CTCTCATGTA TCAATTGGCT GCTCGAATGG GGACCAAAAT GACGGGAGGC CTAGGATTTC ACGAAGTCCT CAATAATCTA ATCTCTAGGA TTTCACTGGA TCACCCCCAT CATACTTTGT TCATTATACT GGCCTTAGCA AATGCGAACA AAGATGAATT TTTGAGCAAA CCAGAGACAA CAAGAAGGAG TCGAATAACC AAAAGTACAT CTAAAGAAAA CTCTCACCTT GATGAGGATC GAACAGAGGC TGCAACCAGA ATCATCCACT CCATCAGAAG TAAGCGATGT AAGATGGTGA AGGACATGGA GGCGCTCTGC GATGCCTACA TCATCTTGGC AAACATGGAC GCCTCTCAGT GGAGGGCTCA GAGAAAAGGC ATCAATATTC CAGCCAACCA GCCAATCACT AAACTGAAGA ATTTAGAAGA TGTTGTTGTT CCCACTATGG AAATTAAGGT TGATCCCACA GGAGAGTATG AAAATCTGGT GACTATAAAA TCATTTAAAA CAGAATTTCG CTTAGCTGGA GGCTTAAATT TACCCAAAAT AATAGATTGT GTGGGTTCTG ATGGCAAGGA AAGGAGACAG CTTGTGAAGG GCCGTGATGA CCTGAGGCAA GATGCTGTCA TGCAGCAGGT CTTCCAGATG TGCAATACAC TACTGCAGAG AAACACTGAG ACTAGAAAGA GGAAACTGAC TATCTGCACA TACAAGGTGG TTCCCCTTTC TCAGCGAAGC GGTGTTCTCG AGTGGTGCAC AGGAACCGTT CCTATTGGTG AATATCTTGT TAACAGCGAA GACGGTGCAC ATAGAAGATA CAGGCCAAAT GATTTCAGTG CCAATCAGTG CCAAAAGAAA ATGATGGAAG TGCAGAAGAA GTCTTTTGAA GAGAAATATG ATACCTTCAT GACGATTTGC CAAAACTTTG AACCAGTTTT CCGTTACTTC TGCATGGAAA AATTCTTGGA CCCAGCTGTT TGGTTTGAGA AACGATTGGC ATATACACGC AGTGTGGCCA CATCTTCTAT CGTCGGTTAC ATCCTTGGAC TTGGCGACAG GCACGTACAG AATATCTTGA TAAACGAGCA GTCGGCAGAG CTTGTGCACA TAGACCTGGG AGTGGCTTTT GAACAGGGGA AGATCCTTCC CACTCCAGAA ACAGTTCCTT TTAGACTCAG CAGAGATATT GTGGACGGGA TGGGCATCAC CGGTGTGGAA GGTGTCTTCA GAAGGTGCTG TGAAAAAACG ATGGAAGTTA TGCGGAGTTC TCAGGAAACC CTGCTGACCA TTGTAGAGGT TCTTTTGTAC GATCCACTCT TTGATTGGAC TATGAATCCT TTAAAAGCTC TGTATCTACA GCAGAGACCA GAAGATGAGT CCGACCTCCA TTCCACCCCC AATGCAGATG ATCAAGAATG CAAACAAAGT CTTAGTGATA CTGACCAGAG TTTCAACAAA GTAGCTGAGC GTGTCTTGAT GAGACTGCAA GAGAAACTGA AAGGCGTGGA GGAAGGCACT GTGCTCAGTG TGGGTGGACA GGTGAACTTG CTTATCCAGC AGGCCATGGA TCCCAAAAAT CTCAGCCGAC TCTTCCCAGG ATGGAAAGCT TGGGTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
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