pECMV-Rb1-m-FLAG
$84.00
In stock
SKU
PL78753
100μg liquid plasmid(Instantly):$105
| name | pECMV-Rb1-m-FLAG | another name | apECMV-Rb1-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGTGGAGTC AATAGCGAGC TCGTTTAGTG ACCGTCAGAA TTTTGTAATA CGACTCACTA TAGGGCGGCC GGGAATTCGT CGACTGGATC CGGTACCGAG GAGATCTGCC GCCGCGATCG CCATGCCGCC CAAAGCCCCG CGCAGAGCCG CGGCCGCCGA GCCCCCGCCA CCGCCGCCGC CGCCGCCTCG GGAGGACGAC CCCGCGCAGG ACAGCGGCCC CGAAGAGCTG CCCCTGGCCA GGCTTGAGTT TGAAGAAATT GAAGAACCCG AATTTATTGC ATTATGTCAA AAGTTAAAGG TACCCGATCA TGTCAGAGAA AGAGCTTGGC TAACTTGGGA GAAAGTTTCA TCCGTGGATG GAATCCTGGA AGGATATATT CAAAAGAAGA AGGAACTCTG GGGCATCTGC ATCTTTATCG CAGCAGTTGA TCTAGATGAG ATGCCATTCA CTTTTACTGA GCTACAGAAA AGCATAGAAA CCAGTGTCTA TAAATTCTTT GACTTATTAA AAGAAATCGA TACCAGTACC AAGGTTGATA ATGCTATGTC AAGACTATTG AAGAAGTATA ATGTGTTATG TGCACTCTAC AGCAAATTAG AACGGACGTG TGAACTTATA TATTTGACAC AACCCAGCAG TGCGTTATCT ACTGAAATAA ATTCTATGTT GGTGCTAAAA ATTTCTTGGA TCACTTTTTT ACTAGCTAAA GGAGAAGTAT TACAGATGGA AGATGACCTG GTAATCTCAT TTCAGCTAAT GTTGTGTGTA GTTGACTATT TTATTAAGTT CTCACCTCCT GCACTACTCA GAGAGCCATA CAAAACAGCT GCAATCCCCA TTAATGGTTC ACCTCGAACA CCCAGAAGAG GTCAGAACAG GAGCGCTCGG ATAGCAAAAC AACTAGAAAA TGATACGAGG ATTATCGAGG TTCTCTGTAA AGAACACGAG TGTAATATAG ATGAGGTGAA AAATGTTTAT TTCAAAAATT TTATCCCTTT TATAAATTCA CTTGGAATTG TATCATCTAA TGGACTTCCA GAGGTTGAAA GTCTTTCTAA ACGCTATGAA GAAGTTTATC TTAAAAACAA AGATTTAGAT GCAAGACTGT TTTTGGATCA TGATAAAACA CTTCAGACTG ATCCTATAGA CAGTTTTGAA ACAGAGAGAA CGCCACGAAA AAACAACCCT GATGAAGAGG CAAACGTGGT TACTCCACAC ACTCCAGTTA GGACTGTTAT GAATACTATT CAACAATTAA TGGTGATTTT AAATTCTGCA AGTGATCAGC CATCAGAAAA TCTGATTTCC TACTTCAATA ATTGCACAGT GAATCCAAAA GAAAATATCC TAAAGAGAGT AAAGGATGTT GGGCACATCT TTAAAGAGAA GTTTGCTAAC GCTGTGGGCC AGGGCTGTGT TGACATCGGA GTACAGCGAT ATAAACTTGG AGTCCGATTG TATTACCGTG TGATGGAATC CATGCTTAAA TCAGAAGAAG AACGTTTGTC CATTCAGAAT TTTAGCAAAC TCCTAAATGA CAACATCTTT CATATGTCTT TACTGGCCTG TGCTCTTGAA GTTGTAATGG CTACGTATAG CAGAAGTACA TTGCAGCATC TTGATTCTGG AACAGATTTG TCCTTCCCGT GGATTCTGAA CGTACTTAAT TTAAAAGCCT TTGATTTTTA CAAAGTGATT GAAAGTTTTA TCAAAGTGGA AGCCAACTTG ACAAGAGAAA TGATAAAACA TTTAGAAAGA TGTGAGCATC GAATCATGGA ATCCCTTGCA TGGCTTTCAG ATTCACCTTT ATTTGATCTC ATTAAGCAGT CCAAGGATGG AGAAGGACCT GATAACCTTG AACCTGCTTG TCCTCTCAGC CTGCCTCTCC AGGGTAACCA TACTGCAGCA GATATGTATC TTTCTCCTCT AAGATCTCCA AAGAAAAGAA CTTCCACTAC ACGTGTAAAT TCTGCTGCAA ATACAGAGAC ACAAGCAGCC TCAGCCTTCC ATACTCAGAA GCCATTGAAA TCTACCTCCC TTGCCCTGTT TTACAAAAAA GTGTACCGTC TAGCATATCT CCGACTAAAT ACACTCTGTG CACGCCTTCT GTCTGACCAC CCAGAGCTAG AGCACATCAT CTGGACTCTG TTTCAGCATA CATTGCAAAA TGAGTATGAG CTCATGAGAG ACCGACATTT GGACCAGATT ATGATGTGCT CTATGTATGG CATCTGCAAG GTGAAGAACA TCGACCTCAA GTTCAAAATC ATCGTCACTG CCTACAAGGA TCTTCCTCAC GCTGCCCAGG AGACCTTTAA ACGTGTTTTG ATCAGAGAAG AGGAGTTTGA TTCCATTATA GTATTCTATA ACTCCGTTTT CATGCAGAGA CTAAAAACAA ATATTTTACA GTATGCCTCC ACCAGGCCTC CTACCTTGTC ACCAATACCT CACATTCCTC GAAGCCCTTA CAAGTTTTCT AGTTCACCCT TACGGATTCC TGGAGGTAAC ATCTATATAT CACCCCTAAA GAGTCCTTAT AAAATTTCAG AAGGTCTGCC AACACCCACA AAAATGACTC CGAGATCAAG AATCTTGGTC TCAATTGGTG AATCATTTGG GACATCTGAA AAGTTCCAGA AAATAAACCA GATGGTGTGT AATAGTGACA GAGTGCTCAA AAGAAGTGCT GAAGGCGGCA ACCCCCCCAA ACCACTGAAA AAGCTGCGCT TTGACATCGA GGGAGCCGAT GAAGCAGATG GGAGTAAACA TCTCCCAGCG GAGTCCAAAT TCCAACAGAA ACTGGCAGAA ATGACTTCCA CTCGAACACG AATGCAAAAG CAGAGAATGA ATGAGAGCAA GGATGTCTCA AACAAGGAGG AAAAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CCAATCTTGG TTACT
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