pECMV-Pcnt-m-FLAG
$84.00
In stock
SKU
PL78710
100μg liquid plasmid(Instantly):$105
| name | pECMV-Pcnt-m-FLAG | another name | apECMV-Pcnt-m-FLAG |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAGACGAG CAGGAGCAGC GGCGCAGGAA GGTGGAGGCT GGGAGAGCCA AGCTTGCTAA CTTCCGACAG AGAAAAACAA AAGGTGACTG TCCGAATTCG AAGAAAAAGA CGGCGAAGAG GAAGGGTTCG GCTGTCCATG CGTCTGTCCA GGAGGAGGGT TCGGTAGCCA CTCCCAACAG CGAGCTCCCC CAAGGAGGGG CGGTCTTTGA AAGCCCATCA TGCAGCAACA CCCTGGAGGG GACCAGAGGG GCATCTGCAG CTCAGGAACA AGAAGACTGT GAACTGGATG TGACTGACCT GCAGGGACAG CAGCAGACAC AGCCACCGCC ACCCCAGACA GCCCACAGCC TGGAGCTAGA AGCGCTGCGC TTGAGCCTGA ACAACATGCA CACAGCGCAG CTGGAGCTGA CGCAGGCCAA CCTGCAGAAG GAAAAGGAGA CAGCGTTGAC GGAGCTTCGG GAAATGCTCA ATGGCCGGCG GGCACAGGAG CTGGCCCTGC TGCAAAGTCG GCAGCAGTGC GAACTGGAGC TGCTGCGAGA GCAGCACGCC CGCGAGAAGG AAGAGATGGC GCTCCGCAGC GGGCAAGAAG CAGCTGAGCT GAAGGAGAAG TTACGGTCAG AAATGGAGAA AAACGCCCAG ACGATCGAGA CCCTGAAGCA AGATTGGGAA TCCGAAAGAG AGTTATGTTT GGAAAACCTC CGCCAGGAAT TGTCGTTGAA GCATCAGTCG GAGATGGAGG GTTTGCAGAG CCAGTTTCAG AAGGAATTGT CAGAACAGAA GGTTGAATTG GAGAAGATTT TCCAGGCCAA ACATGAGGCT GAAGTTTCCC TGAAGAACCT GGAGGCTCAA CACCAGGCGG CCATCAAGAA GCTGCAAGAA GACCTGCAGT CAGAGCACTG CCAGTATTTA CAAGATCTAG AGCAGAAATT CCGAGAAAAG GAAAAAGCAA AAGAGTTGGA GTTAGAGACC CTTCAGGCAT CCTATGAAGA CCTAAAGGCA CAGTCCCAAG AAGAGATCAG ACTCCTTTGG TCCCAGCTTG AGTCTATGAA AACCAACAGA GAGGAACTGA ATGGGTCATG GGATCCAGTG CTGGCCCAGG CCTCTCACCT GGAGGAGCTG GAGCACCTGC GGTCGGGTTT CGCTCAGCAG CAGCAGCAAG AGCGAGCCCA GCACGAGTCT GAGCTGGAGC ACCTGAGGGT CTATTTTGAG AAGAAGTTGA AGGATGCCGA GAAGACATAC CAAGAAGACC TGACTGTGTT CCAGCAAAGG CTGCAGGAGG CAAGGGAGGA TTCTCTGGAG TCTACAGAAA TCAGTTCGTC CTGTGTGCTT CCGGAGGAGA CATCTGGGAG AGAAGGAAAG GAGCCACCAG ATCCACTTGA TCTTCAGCTT GGGCAGCCGA AGGTCCAGGA GAGTCTGGTT GAAGACTGTC AGGTGAAACT CAGTAAAGCC GAGGAGAAGA TTCAGCAAAT GAAAGAGGAG TTCCAAAAGA AGGAAGCCGA GTGGGAGCTT TCCCGGGAGG AACTAAAGAG AGAAGCTGAA GAGAGATTAG CCTCCATGTT TCTGGAGCTG AGAGAAAAGG CTGAGTCTGA GAAACTGTCC ATAATCAGCA GGTTTGAACA TCGGGAGTCC AGCATGAGGC ACCTGCAGGA CCAGCAGGCC GCCCAGATCT TAGACCTGGA GAGATCTCTG ATGGAGCAGC AGGGTCACCT GAGGCAGCTG GAACAAGAAC TCACTCGAGA TGACCTTCTG CCATGCAGCC AGTGTGGCCA GGAGCCCGCC ATGGCCCAGG AGGAGAAGAA TGGCGCGCTC CTCCGAGAGA AGGAAGACTG TGCTCTGCAG CTGCTGATGG CCCAGAACAG GTTTTTGGAA GAGCGGAAAG AGATCATGGA GAAGTTTGCC AAGGAGCAAG ATGCCTTTCT TCGAGACGCC CAGGAGAAGC ACAACCACGA GCTGCAGCTC CTTCAGCAGG GACACCAGCA GCAGCTCCTG GCTCTGAGGA TGGAGCTTGA AACCAAGCAC CGCTCTGAGC TCACAGAGCA GCTCGCCTCC TCCGAGAGCA GGCGGCAGGC GCTCCTTGAG ACTCACGTGG CCGAGCTGCA GGTAAAACAC AATGCTGAAA TTAGCGCCTT GGAGAAGAGA CACCTCTCCA ACCTGGATGA GTTAGAGTCC TGTTACGTGG CCGACGTTCA GACTATTCGG GATGAGCACC AACAGGCTTT GGAGCTCTTG CGAGCGGAGC TGGAGGAGCA GCTACAGAAA AAGGAGTCTT GTCACAGAGA GATGTTGACG CAGGAGCTGG AAAACCTGAA ACGCCAACAT GCTGAAGAGC TTCAGTCCGT AAGAGATAGC CTGAGAATGG AAATGAGTGC CCAGCACATA GAGAACGGGA AAGGGCCGGC CGCTGACTTG CAAGGAGCAC ACCAGCAAGA CCCGGCCATG GCTCTGCACA ATGAAGGGCA CCTGCTGGTG GAGGATGGCG ATGCAGTCTT GCGTAGTGTG GACGCTGAGG GCCTGCTGCA CCAAGCTGGA CCTCAGGAGC TGGGAGACGC GCACACAGTT GAAATGCAGA AGTCACAAGC AGAGTTGGCC AAACCCCAGG AGCTGCAGGC ATCACAGGAC CAGGTTGCAC AGGTGAGAGA CAAGGTCTTC CTGCTGAACC GGGAGTTAGA AGAGTGCCGA GCGGAGCTGG AGCAGCTACA GCAGAGGCGG GAACGGGAGA ACCAGGAGGG CACCACGCTG ATCTGCATGC TCAGGGCTGA CCTGGAGCTG GCTCAGGGCG AAGGGAAAGC CCTCCGAGAT GCCCTGAGGA GACTTCTCGA TCTGTTTGGG GACACACTGA AGGCAGCTGT GACCCTAAAG AGCCGGATCA GTGAGCGTGC GGGGCTCCTC CTGGACCACG AGGATGCTGC AGATACAAGT GACGCCCGGT TAGCAGCTGC CGCCCTTGGC GATATGTGGT CTGACGAGGG TCTACTGGAG ATAGACAGAA CGTTGCCAGA AGGGGCAGAG ACATCTTCCG TGTGTGAAAT TAGCAGTCAT GTGTGTGAAA GCTTCTTCAT CAGCCCTGAA AACACACTGG ACTGTGAGCA GCCAATTAGG AGAGTCTACC AGAGCCTCAG CACGGCCGTG GAGGGCCTCC TGGAGATGGC CCTGGACTCC TCCAAACAGC TGGAGGAAGC TCGCCAGCTC CATCGCTGTG TCGAGAGAGA GTTCCGTCAC AGGAACGAGG AGATGGCCCA GGCTATGCAG AAGCAGCAGG AGTTGCTGGA GCGCCTGAGG GAGGAGAGTG CGGCCAAGGA CAGGCTGGCT CTGGAGCTGC ACACGGCTAA GGGCCTCCTT GAAGGATTCA AGGTGGAGAA AGTGGATCTG CAGGAGGCGC TGGGCAAAAA GGAGGAGTCG GAACAGCAGC TGATCCTGGA GCTAGAGGAT CTGCGGAAGC AGCTGCAGCA GGCTGCCCGC GAGCTGCTCA CTCTGAAGGA GGAGAAGTCG GTCTTGTGGA ACCAGAAGGA AACTTTGACT AATGAAGCAA AAGAAAGAGA AGCTGCACTG CAGGAGGAGG TGGAATCTCT TACCAGAGTC CAGTGGGAGT CCAGGAAGCA GTCTGAAAAG GACCGCGCCA CCCTACTCTC TCAGATGCGC GTCTTGGAGT CTGAGTTGGA GGACCAGCTT GTGCAGCACC GGGGCTGTGC CCAGCTGGCT GAGGAAGTGG CTACCCTGAA GCAGCAGCTG GCTGCCCTAG ACAAGCACTT ACGTAGCCAG CGTCAGTTCA TGGATGATCA AGCTGCCGAG CGTGAGCATG AGCGGGAAGA GTTCCAGCAG GAGATCCAGA GGCTGGAGGG GCAGCTCCGC CAGGCAGCCC GGCCACGGCC TCCCGGGCCC CGGGACAGCC AGTGCGTCCA GTTAGACGAG GAGGTGGAGT TGTTACAAGA AAAACTGAGA GAAAAATTAG ATGGGTTTAA TGAATTGGTC ATAAAGAAAG ATTTTGCCGA TCAACAATTG CTAATCCAAG AAGAAGAAAT TAAGCGCCTC GAGGAGACAA ATGCCAGCAT ACAAAGACAA ATGGTCCAGC TCCAGGAAGA ACTGGAAAAA CAGAAGAAAA GCATGGAAGA ATTAAAGGAA AAAGAGATAC TAAAGCAAGA GAACATGGGT GACTTACTGC TAACAACTGT ATCACGGTCC GGGCTGGATG AAGCTGGGTG TCCTATGCTT CCCCAAGGCA GCTCTTCTAG GGGTCCGGAA GCCCAACCAG ATGTAACAGA GAGGGCACTC TTACAGCACG AGAATGAGGT TGTCCACAGG AGAAACTCTG AGATTGATGA GCTGAAAAGC CTCATCGAGA ACTTACAGGA AAACCAGAGG CAATTACAGA AGGATAAAGC AGAGGAAATC GAGCAGCTCC ACGAAGTCAT TGAGAAGCTG CAGAGCGAGC TGAGCCTCAT GGGACCCAAG GTGCACGAAG TCAGCGATCC TCAGGCTGGC AGCCTCCACA GTGAGCTGGC CTGCCTCCGG GGGGAAGGCC TGGGTGGACA GGCACTACGG AGTGAGCTGC AGGCTGCGCA GGCAGCCAAG GAGGTGTTTG GCCAGCTCCT GGCAGACCAG GCCCACGGGC ACAGCCAGGC TCTGGAGGCC CTGCAGCAGC GTCTCCAGGA CGCAGAGGAG GTTGCTGCAA GACACCTGGC TGAGCTAGAA CACTGTGTGG CCCTCAGAGA GGCTGAGGTG GAAGCCATGG CCTCTCAGAT CCAGGAGTTT GCAGCTACCC TGAAGGCAAA GGAAGCAATC ATCGAGCAGA GAGATTTAGA GATTGATGCC GTGAACAAGT GGAAAGTAAG CCATTCCCTG GAGCTGGAGG CCATCCTTTT GGCTTTGGCT CACTTCCGTC ATGCTCTGGA ACAGCAGACC TGTGCCACCC CGGACGAGCC CCCTGAGCTC CGGCAGCTGC GAGTGCAGTG TGCTCGCCTC AGCCACCAGC TACAAGTGCT GTATCGGCCT TTCCTCAAGT GCCGGATGCA GTTGGACCAG CACCAGCCTC ATGTGGCATC TATTGGCTGT GCAAACCCTT GTGCAGATGA TGAACTGGAG CAGGAGGGAG TCAGCAACAG GCTGGCCTTG GCTCCCCACA GCCTGGCCGC ACAGGCTAAG GAAGAACTGG AAGACTGTCC CCTGGGCAAG GCCAATCTCA TGGCCCAGGT GAGACAACTT CAGGAGGAAC TGGACCACCG CGTGCATTCC GTGGCCTCCA GGGACACCAA CTCGGAAACT TGTAAATTAC AGCAGCCAAA CCTGTCGGAA AATGGGCCAC GTAATCATTG TTGTAACGGT GAAGAAAGTA AGCCATCGCC TCCCGATGAT GTACTTAATA TTGCCAAAAC CACATGGGAT GTAATTGATA TTATTAAAAA TCAGGATTTG TTGGTCCAAG TTGAAATGCC AGATTTCCCC ACTCAAGAAA AGTTAACATC CCAAGGTGGA CCTTTCTCTT CCCAAGCCAG TGGGCACAGC GGCTCCCTCC TCCCAGAGGA GGCGGCTGAG CCCCAGCAGG ACCCCGTGAG GGCACTGGAC CTGTCGTCCT GGAGCTCCCC TGAAGTTGTC CGGAAGGACC CATCCTTGGA GCCTCAGCAC AGCCTGCCCC TGACCCCGGG CGTGGGCACA GTGAGCCTGC ACAGTGTGGA CATCTCACCA GACTGGACAG ACCCGCTGCT GCAGGCAGAC GTGTCGGGGC TGCTTTGTTA CCCGGGCAAG TCTGCATCAG GCCAGGCCCC ACTGTGGGCA GTGGCCCCCT CGGCTGGGAA GCACCATGCG GAGAGGACAG CCACGGAGAA GGACGTCGAA GACTTTATCG TAACATCTTT TGATTCTCAA GAATTGTTAA CATCCCCTTC TCATGAGTTA GCGAGAAGAA GCGATGGGAG CAGGAAAAGT GATGGCCCAG ACATTGCTAT GATGTTGACC CTGGGCTCAG AAGGGTCTGA GACTCCCACC ACTGACCTAG TCGCTGCTGC TGCTGCTGCT GTTCCTTTCT CTAGAAGGTT TGTCCAGTCA CCAGGAGCAA TGAAGGAGAA GGAGATCCAT GCAAAGCAAA TGAAGGCTTT GCTGCAGATG GTGTTTGATG AGAGCCACCA GATACTGGCC TTGTCAGAAA GCCAGGACCC CTCTAGTGCA CTGAACAAAG GTGAACCACG TGACCCCCTG GATGGCTTCC CGAGAGACAG TCAGGCCCTT TCGGAGGTGA CAACTGACAA AGGAGAGAAG GAATCTTTGG AAACACACCT TACGTGGAGT GAGGAGCTTT TGCGGGCCAT ACAAGAAGTG TTTGCCAGGG AGCAGGAGAA GGCTGAGCTG CAGCCTAGAC CTTATGGTTC AAACCTAGGA GATTATAACT CCCTGGTCCA GAGGCTGGAG AAGGTTATCC AAGAGCAGGG AGACCCGCAG AAGGTCCAGG ACCACCTGTG TCTGTCAGAC AGGAGCAGCC TGCTAGCTGA GATTCAGGCC CTGCGTGCTC AGCTCCGCAT GACACACTTA CAGAACCAAG AGAAGCTGCA GCAATTGTGT GCAGCCTTGA CGAGCACAGA GGCCCGAGGG AGCCAGCGGG AGCACCAGCT ACGAAGACAA GTTGAACTTT TGGCGTACAA AGTGGAACAG GAAAAGTGCA TAGCAAACGA GTTACAGAAA ACCTTGAGCA AAGAACAGGA GACGGCGAGC GACGTGCGCA AGCGCCTGGT GGTGGAGCAG AATGCCGTCC AGGACTTGAA GTCCGAGCTG CACGCCTGTA AGCAGGAGAA CACCAGCCTG CTGGAGTCGC TGGACAAGGT GCAGCAGGAA GTCCTGCGGC TCAGGTATGG CCATGTGACG TGGTCCTACT GTGGAACTTC TTCTGCTTCG GCTGGCAGAG CTGTGCTGGA CGGGAAGGAG AAGGAGCTGA AGGTTGTGCT GGAGGAGCTG GAGTCTGAGC GCGGAAAGGG GCAGGCCCTC CAGGCCCAGC AGGAGGAGCA GCAACTGCGG TACCTGCAGA GGGAGGGCCA GAGCTCCAGG GCCCTGGAGG AGTTGAAACT ATCTTTGGAG AAGCAGCTTG CTCAAAACAA CCAACTGTGT GTGGCTTTAA AACACGAGCG TGCGGCCAAA GACAACCTGC AGAAGGAGCT GCAGATCGAG GCCTCGCGCT GCGAAGCGTT GCTGGCACAG GAGAAGGGCC AGCTCTCAGA GCTGCAGAAG AGCCTGGAGG CTGAGCGGAG CCGCTCACTC GAGCTCTCAG AGGCCCTACA GCACGAGCGC CTCCTGACAG AGCAGCTGAG CCGCAACTCG CAGGAGGCCT GTGCCCGCCA AGAGACACAA GTGCAGCACG CTCTGCTGCG AAAGCTGAAG GCAGAGAAGA CTCGTGCGTT GGAGCTGGAG GCCATGCTTG AAAAGGTGCA GAAACAGGCT GCCCACACCC AGCAGCAGCT GGAGGCTCAG GCTCAGGAGC GCTGCGTAGA GCTCAGGAGA GAGAAGGAGC GAGAGTTGGA GATACAGCGT CAGCGTGATG AGCATAAGAT CGAGCAGCTT CAGCGTCTGG TGAGGGAGCT GAGGTGGAAA GAGGAGGTGT CCGGAGGAAA CGGCCCTTGC CGGGGCTCCC CCGGCCGGGG TTCTCTGGAG AGGGACCAGT TCCAGGAGCA GCAACAGGAA CTGGAGAAGA TCCGGCAGCA GCTACTGTGT GCAGCTGGCC TCCTCACAAG CTTTACCAAC CACACGGTAG ACAGGACAAT TAAAGATTGG ACGTCATCAA ATGAGAAGGC GGTGTCATCC TTAATGCGCA CGCTGGAGGA GCTGAAGTCT GAGCTGAGCA TGCCCACCTC CTTTCAGAAA AAGATGACTG CAGAACTCCA GGTCCAGCTC ATGAATGAAC TGCTGAGCGA CAATGATGCC CTCACAAAAG CTGTGGGCAT GGCAACCCGA GAGAAAGCTG AGCTGTGCAG GACCGTCTCC CGGCTCGAGA AGACCCTGAA GCACCACACG CAGAAGGGCT GCGTTCTTAA TAGGCAAAGC AAGTCTTCAT TGAAGCAAGA TGGGACAGAT TTGCAGAGTT CTCTACGGCA CTCAGACCCA GAGTGGCACT CGCAGACTAC AAGTGGGGAC ACAAACACCT GCAATATCAA AATGGAAAAG CTGTACCTGC ACTATCTGAG AGCAGAGAGC TTTAGGAAAG CTCTGATTTA TCAAAAGAAG TATCTCTTAC TGCTGATTGG TGGATTCCAG GACTCAGAGC AGGAAACGCT CTCCATGATC GCTCACTTGG GGGTCTTTCC TTCCAAAGCA GATAAGAAAA TTACTATGTC ACGTCCCTTC ACCAAGTTCC GCACCGCCGT TCGAGTGGTC ATCGCAGTAT TGAGGTTGCG GTTTTTGGTT AAGAAGTGGC AGGAAGTGGA TCGGAAGGGA GCCCTGGTAC ACCCCAAAAG CACCCGCCAC GGACACCGAA CATCCCAGAG ACAACGTTCT CCATCAGGAC CCAGAGCGTC TCTGCCAACC CGAGACACTT CCTCAGGGCC TACCAAGGCC TCTCGGCACA GCCCCAGATC TGCGGCAGCC GGCTCACCGG GCAAGGAAAG ATCAACTTCG ACACCAAGTT CAAGGCTAGA AAGATCCCTA ACTGCTTCTC AAGATCCTGA GCACTCCTTG ACAGAATACA TCCATCATTT AGAAATGATC CAGCAAAGAC TTGGGGGGTT GCCGCCAGAT TCTACTCAGA AATCCTGCCA CCAGAAGATT AAACAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
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