NM_001195633.2;4732475F16; 5430411K18Rik; AI661957;AW456499
100μg liquid plasmid(Instantly):$294
| name | pEnCMV-EPG5(mouse)-Myc-FLAG-SV40-Neo | another name | apEnCMV-EPG5(mouse)-Myc-FLAG-SV40-Neo |
| Insert Size (bp) | 7719bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCGAGGCT GTGAAGCCCC GCCGGGCCAA GGCCAAGGCC AGCCGCACCA AAGGCAAGGA AAAGAAGAAG CATGAAGCCC TTCAGACTTG TGATGCAGGT CCCCTGCCAG AGACCTGTCG CGAGCAGGAG AGCCCGTGTC CAGCTTCTGA ACTCAAAGGA GATGACCTGA AGTCGTCTGC TGACCCCCAG CTCCACAGTG ATGTGTGTGG ATGGAATGAA AGTGAGATGT TTGATATACC ACTCACCTCC TTGACTATAG GGGATGAAGG TCCCCCGGTA CAGGACACAG AGGACCTAAA AGAAAGGGGA GAGGTCACAG CCGGTGATGG AGATGATGAA ATGGAGTTGA AGGTGGACCC TGGGGACAAC GTTATAGCTA AAGGTGAACC TTGTAAGAAC TTCCCGGAAG TAGAGGACCA CACACTCATC CAGTGTGGGC CTCCAGAGAG CACACTGCAG CCTGACTTTC CCTGCACTCA GCAGGCAGTG GAGGGCTCAC ATGCCAGAGA ACATCCGACT AGGAAGCAAG ATGAAGCCGC CCTCGGCTGT TCAAAGGTGT TTCAGAATGT TAGCTTGCAC AGTTCTTATG AAGCCAAAGA AGTTTCTCAG CCACCGAGAG TGAAGAAACT ATATCCTGAG TTGCCAGCTG AAATTGCTGA AGTACCAGCT CTGGTGGCAG TGAAGCCATT GCTCCGTAGT GAGCGACTCT ACCCAGAACT CCCGTCTCAA CCAGAAGTGA CACCCTTCAC TAAAGAACAG CTCAAACTCT TGGAGCCTGG CTCATGGCTG GAAAATGTTG CATCATATGT AGAAGAATTC GATAACATTG CTCATCAGGA CAGACACGAG TTTTATGAGC TGCTTTTGAA TTACTCACGG TGCAGGAAGC AGTTGCTGTT GGCTGAAGCT GAGCTGCTCA CTCTGATGTC TGACTGCCAC AGTGCCAAAA GTCGACTGTG GCACTTTAAG GATGAGCAGA TGGCCGTACA GGGCATCTGT GCAGATCAAG TGAAAGTCTA CGGGCATCAC CACTACCAGC GAGTAGAGAT GAATGAAAAT GTCCTGGGCG AGCTGAAGAA GCTGTTTGAT GCCAAATCTG AGCACCTCCA CCAGACCCTG ACCCTCCACT CGTACACTTC TGTGCTCTCG AGGTTGCAAG TGGAGTCCTA CATCTTCACA CTGCTCAATA GCTCAGCTGC TCTGAGATCT TTAGCAGTTT ATCAGGCAGA CCAAGTTCCT AAACTGACAG AAAGCATCCC CTCTGATGTG TGTCAGCTGA AGGAGTGCAT TAGTGTCTTG TTCATGTTCA CCAGGAGAGT GAGTGAGGAT GCCCAGTTCC ATGAGGACAT CCTGCTCTGG CTGCAGAAGC TGGTGTCCGT GTTACAACGA GTCGGCTGTC CTGGGGATCA CTTCTTTCTC CTGAACCACG TTCTCCGCTG CCCAGCTGGC ATTCGTAAAT GGGCTGTTCC TTTTATACAG ATCAAAGTGT TGAATAACCC TTCAGGGGTC TTTCATTTTA TGCAGTCCCT TGCCCTGCTG ATGTCTCCTG TCAAAAACCG AGCGGAGTTT ATGTGCCACA TGAAGCCCAG TGAGTGGAAG CCATCATCTT CAGGGCCAGC ATCTGGGAAC TGGACACTTG TAGATGAAGC GGGGGAAGAG GATGAAGACC CTGAGACCAG TTGGATTCTC CTTAATGAAG ACGACTTGGT CACCCTTTTG TCCCAGTTTC CCTTTCAGGA GCTCTTTCAG CATCTTCTTG GGTTTAAAGC CAAAGGTGAT TATTTACCTG AAACAACAAG GCCCCAAGAG ATGATGAAAA TTTTTGCCTT TGCCAACTCA TTAGTGGAGC TTCTGGCTGT GGGACTAGAC ACCTTTAACA GAGCCCGCTA CAGGCAGTTT GTGAAGCGCA TTGGATACCT GATCAGGATG ACCCTTGGCT ATGTCAGTGA CCACTGGGCT CAGTATGTGA GCCACAGCAC AGGCGCGGGG CTAACACCGC AGCCCTACTC CATGGAGAAA CTGCAGGTGG AATTTGATGA GCTGTTTCTC AGGGCTGTCC TCCATGTCCT GAAAGCCAAA AGACTTGGCA TTTGGCTGTT CATGTCTGAG ATGCCCTTCG GCACGCTGTC GGTCCAGATG CTCTGGAAGC TCTTGTACCT CATGCACCAG GTGGAGAGTG GGGACCTGCA GCAACTCTGT GCCTCCCTGC AGCCGGCCGA GTGCAAGAGG CGACTCCAAG ACCCAGAACA TTTTGCGAGC TTTGAGAAGT GTCTTTCTTC CATCAACAGC TCAGAGGAGA TCTGTCTCCT CACGGCCTTT GCTCAGATGG CTCGAGCCAG AAGAACCAAT GTGGATGAAG ACTTCATAAA AATCATTGTC CTGGAGATCT ATGAGGTATC TTATGTCACA CTGTCTACCA GAGAGACTTT CTCAAAGGTT GGTCGAGAGC TTCTGGGAGC CATTGCAGCT GTTCACCCTG AGATTATCTC TGTCCTTTTG GACAGAGTGC AGGAGACCAT TGACCAAGTT GGAATGGTTT CTTTATACTT GTTTAAAGAA CTGCCTTTGT ATCTTTGGCG ACCTTCTGCC CCAGAGATAG CTGTGATTCG GGACTGGTTA TTGAATAATA ATCTGACAGC AGTGAAGAAC AAGTTGGCCT GTGTGATCCT AGAAGGACTG AATTGGGGAT TTACTGAACA GGGTACCCTT CATTTGGATC AAGCATTGCA CACGGAAGTG GCCTTACTGG TTCTCGAGGC TTATCAGAAA TACCTGGCAC AGAAACCTTA TACTGGGCTT ATTTCTGAAA GTATGAAACA GGTTTCCTAT TTGGCCAGTA TTGTTCGATA TGGAGAGACT CCTGAGACCT CATTTAACCA GTGGGCCTGG AATTTAATCT TGAGGTTAAA ATTGCACAAA AATGATTTTG GAAGACAGAA CTTTCCAGTC ATTCCCTTCT GCAGCACAGT ACCTGACATG ACAGAATCGT CCATGTTTCA CCCTCTCTTG AAGGCTGTGA AATCAGGCTT GCCCATTGGC TGCTATCTCG CTTTAGCTGT GACAGCTGTT GGCCACAGCC TTGAGAAGTT CTGTGCAGAA GGCATCCCAC TGCTGGGCGT GCTGGTCCAG TCACGGCATC TGAGAGCAGT GGTCCATGCC CTAGATAAGA TCCTGCCTGT GTTCTACCCT TACCAGTGCT ACCTTCTGAA GAACGAGCAG TTTTTGTCGA ACCTTCTCCT CTTCCTACAA TTGGACAGCG GTGTTCCCCA GGGGGTCACA CAGCAGGTCA CCCACAGGGT GGCCCAGCAT CTGACAGGAG CCGTCCATGG AGACAATGTG AAACTTCTCA GCAGCATGAT CCAGGCACAC ATATGTGTAA GCACTCAGCC GGATGGAGTG GGTCCTGTGG CTGTGTTGGA GTTCTGGGTC CAGGCTCTCA TAAGCCAGCA TCTGTGGTAC CGAGAACAGC CCATTCTCTT CCTCATGGAC CACTTGTGTA AGACAGCGTT TCACCTGATG CAGGAAGATT GTGTGCAAAA ATTGCTCTAC CAACAGCACA AGAATGCTTT GGGTTACCAC TGTGACCGGA GTCTGCTCTC TTCCTTGGTG AACTGGATTG TGGCAGGGAA CATCACGCCT TCCTTCGTGG AGGGCTTGTC CACGTCCACA CAGGTTTGGT TTGCATGGAC TGTGTTGAAC ATGGAATCCA TCTTTGAAGA GGACTCTCAG CTCCGAAGAG TTGTTGAAAG GGAATTGGTT ATAAATGCGT TTAGCCCTGA CCAAGCTCTA AAGAAAGCCC AGGTCCAGCT GAAGCTGCCC ATCGTCCCGT CCCTGCAGCG GCTGTTGATT TATCGCTGGG CCCACCAGGC TCTGGTCACA CCTTCTGATC ATCCCCTTTT GCCACTCATT TGGCAGAAGT TCTTCCTCCT GTATCTTCAT CGCCCAGGAC CACAATATGG GTTACCTGTG GATGGTTGTA TTGGAAGAAG GTTTTTTCAA AGTCCCTCGC ACGTTAATTT GTTGAAAGAC ATGAAGAGAC GCCTGACGGA GGTGGCTGAC TTCCACTATG CTGCCAGCAA GGCCCTCCGT GTTCCAGCAG AGGGCAGTGA AGGGACACCA GAAGGGCAGG CTGGCACCCC TGGCTTCCTG ACTTCCCCAG AACTGCACAG GGAACTAGTG AGGCTCTTTA ATGTATATGT ATTATGGCTA GAAGATGAAA ATTTTCAGAA AGGAGATACC TATATTCCTT CTTTACCAAA GCACTACGAT GTTCACAGGC TAGCAAAAGT GATGCAGAAT CAGCAGGACC TGTGGATGGA GTATGTGAAT ATGGAGCGCA TACAGCACGA GTTCCAGGAG ACAGTTGCTC TGTGGACACA GGCCAAGCTT GAGTCCCATG CCGCACCCTG CAGCTCTTCA GCACAGCTGG ATTTCACGGA CCCCTTGCTG GCTAAAGCAA GGGTTCTGAG TAACTTAGAG AAGCACGAGG CTCCACATCC TCCTCTTCTT CTGCACCCCG TGAGGCCTCC CGTGCCACTC ATTCCCTCTG CTGCGCTGTT GACACAGAAG GACAGCACCC AGCTCATGTG CACAGACCTG AATCTGCTGC AGCAGCAGGC CAGGAGTGCC ACTCTGCGGG AGTCGCAGCA GGTTGCCTTG GATGGCGAGC TGTTGGAGAC CATGCCCAAA CAGTACGTTA ACCGGGAAGA TCAGGCCACC CTGCATCTGG AGTGCAGAGG CAGCAGTGGC AAGAAGTGCC AAGGAGCAGC AGTTGTAACG GTCCAGTTTG AAGGCATGAA CAAGAATGAA GCCGTAAGCC AGCAGATCCA TGTTCTGCAG AAAGAAGTGA GGCAGCTCCA AGCGGAAGCT GCTCAGCCCC CGGCACTGAA TGTTGTGGAG GCCGCTGTGC ATGCAGAAAA CCTGATCACG GCCTTAGTGA ACACTTATAA GTTACAGCCT ACACCTGGAG TTCAGAAACT TGGCATTAGC CTTTTCTTTA CTGTTGTGGA CCATGTCAGC GATGAGACCC AGCGCCATCC TCCTACAAGG CAGTTCTTCA CTTCATGCAT CGAGATCTTG GGACAGGTGT TTGTTAGCGG CACTAAGTCC GAGTGCAGGA AGCTGCTCCA GACCATCCTG AAGAACAGGC GGCTCTGCTC GCTGCTGGCT CCTTTCTTCA CGCCCAATGC TGCGCCCGCC GAGTTCATCC AGCTGTATGA GAGAGTGGTG ACTTGTCTGC GCGAGGACAA CAGCGACGTG ATCTTCATGC TGCTCACCAA GTTTGATATT CAGCAGTGGT TAAACAGCAC TAAGCCCCCT CTGTCTGACC GCACCAGGCT CCTGGAGTCC ATTCACTTGG CACTCACTGC CTGGGGCCTT GAACCAGAGG AAGATATTCT GATGCCATTT AATCTTTTCT GCAAGCACTG GACTCACCTC CTACTCTACC AGTTCCCTGA CCAGTACAGT GATGTCCTCA GGCTGCTGGT GCAGAGCTCT GCCGAGCAGC TGCTGAGTCC CGAGTGCTGG AAAGCCACTT TGAGAGCCCT GGGCTGTTAT GCCCCAAGCA GCCAGCAGGG GGCGGCGTCT GTGGAGAGCT CTGGGCTTCA CAGTGCCTCC CGTGTTCTCC TGTCAGACAA GCAGGTAATG GAGACGGTAC AGTGGCTTTC TGACTTCTTT TATAAGTTGC GGCTCTCCAA GTTGGACTTT AAAAGCTTTG GATTATTCTC AAAGTGGAGT CCTTACATGG CTGATGTGAA GACATTTCTG GGATACCTTG TGAAAAGGCT GACTGACTTA GAAATAGCCT CCTTGTCTCA GGACCCAACC GCCAGCAGCA AAGAAGTGCT GAGGTCCCTG CACGCGCAGA TCATCCAGCT CTTTAAGCCC TGGATCCTGG TTTTAGAGGA CGCGGAAAGT AGCCACCAGC GACATTACCC GTGGCTGGAG AGTGACACTG CGGTGGCCTC CAGCATCGTG CAGCTCTTCT CTGACTGTGT CGGCTCATTG CACACGAGTT TTAAAGACAG GCTGTTGCCA GGCGATGAGG GAGCCCTGCG GTTGCACCTG CTGCACTACT GTGAAACATG CACAGCACCC AAGATGCCGG AGTTCATTCT CTATGCCTTC CACAGTGCAT ACCAGAGACT GGAGTGGAAG GACTTGCACC CTGACCAGAG GCTCATGGAG GCCTTCTTTA AGGTGGAACG TGGAAGCCCC AAGAGTTGTT TCTTGTTTTT GGGGTCTGTA CTTTGCAGAG TCAACTGGGT TAGTGTGCTG TCTGACGCCT GGAATCCCAG CCCCCTTCCG GAAACCCAGA GCATGGCTGT CTGCCTCCTT TTCATGATGG TTTTACTAGC AAAGGAGGCT CAGCTCGTTG ACGAGCCAGA TTCACCTTTA CTGAGTCTCC TTGGACAGAC AAGCTCACTC TCATGGCATC TTGTGGACCT TGTGTCATAC CAGAGTGTGT TGGGTTATTT CAGCAGTCAT TACCCACCAT CCGTCGTCCT GGCAAATGAC TGTTCCTCGG AACTGATAGT GAAGCTGTTA AAAGTGTCTG CAGGGCTTTC TGCTCATGCT GACGGCCGGA AGCACGTGGA CATAGTTCCA AAATGCCAGG CTTTCACTCA TCAGATGGTT CAGTTTCTCA GTGCCTTGGA ACAGACTGGA AAAATCACCT TCCCAGCACT GGAGCGGGAA ATATCTAAGC TGTTGGATGA CATCATTATC TTTAACCCAC CCGACATGGA CAGCCAGACC CGCCACATGG CCCTCAGCAG CTTCTTTGTG GAAGTCCTGA TGATGATGAA CAATGCGGCT GTTCCAACAG CAGAGTTCCT TGCTGTCAGT ATTCGGACCT GGATTGGCCA AAGAGTGCAT GGCTTGATAG TGCTGCCCCT CTTAACAGCA GCCTGCCAAA GCCTGGCGTC TGTCCGCCAC ATGGCGGAGA TCACCGAAGC CTGCATCATG GCCTACTTCA AGGAAAGCTC CCTCGATCAG AACTTAGGAT GGGGCCCCGT TCTGGTGTCC CTTCAAGTTC CCCAGCTCAC CGCGCGAGAC TTTCTGGAGG AGTGCTTAGC CCTCGGTAGT TGTTTGACTC TCTATGTCTA CCTGCTTCAG TGTCTGAACA GTGAGCAGAC GGTGAAGAAC GACATGAAAA TGCTCCTCGT CGTCAGCGGC TGGCTGGAGC AGGTGTACCC GAGCTCTGCA CAGGAAGAGG CAAAGCTGTT TCTGTGGTGG CATCAAATCC TGCAGCTGTC TCTGATTCAG TTGGAGCAGA ACGACTCCGT CCTGACAGAG TCCGTCATCC GGATCCTGCT CATGCTGCAG AGCAGGCAGA GCCTTATGGC CGAGGAGAGG CTCAGCTCTG GGATTCTGGG GGCAATCGGG TTGGGCCGGA GATCACCCTT GTCCAACAGG TTCCGAGTGG CTGCCCGAAG CATGGCTGCC TTCCTGTTGG TTCAGGTCCC TGCAGAAGAC CAGATTCGTC TGAAACCCAG CTCTGAGTTA CATCTGGCTC CAAAAGCTCA GCAGGTGCTG ACAGCTCTGG AGTCCATGAC GCTCAGTAAG CAGTATGTGG AGTACCAGGA CCAGATACTG CATGCTCTTC AGTTTATAAG GCATCCTGGC CACTGCCTTC AGAATGGGAA AAGCTTCCTG GCCCTTCTTG TCAACCGCCT CTATCCAGAA GTGCATTACC TGGACAACAT CCGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
