pSPYNE-35S
$128.00
In stock
SKU
PL78531
100μg liquid plasmid(Instantly):$149
| name | pSPYNE-35S | another name | apSPYNE-35S |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35s | Replicon | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GTTAACCGGG CTCAGGCCTG GCGCGCCACT AGTGGATCCA TCGATAGTAC TGTCGACCTC GAGGGTACCG CTCCCGGGAT GGAGCAAAAG TTGATTTCTG AGGAGGATCT TATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCTTCGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCTAAG AGCTCGAATT TCCCCGATCG TTCAAACATT TGGCAATAAA GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT ACTAGATCGG GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGAACC ACCATCAAAC AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC AGGCGGTGAA GGGCAATCAG CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCCAG TACATTAAAA ACGTCCGCAA TGTGTTATTA AGTTGTCTAA GCGTCAATTT GTTTACACCA CAATATATCC TGCCACCAGC CAGCCAACAG CTCCCCGACC GGCAGCTCGG CACAAAATCA CCACTCGATA CAGGCAGCCC ATCAGTCCGG GACGGCGTCA GCGGGAGAGC CGTTGTAAGG CGGCAGACTT TGCTCATGTT ACCGATGCTA TTCGGAAGAA CGGCAACTAA GCTGCCGGGT TTGAAACACG GATGATCTCG CGGAGGGTAG CATGTTGATT GTAACGATGA CAGAGCGTTG CTGCCTGTGA TCAAATATCA TCTCCCTCGC AGAGATCCGA ATTATCAGCC TTCTTATTCA TTTCTCGCTT AACCGTGACA GGCTGTCGAT CTTGAGAACT ATGCCGACAT AATAGGAAAT CGCTGGATAA AGCCGCTGAG GAAGCTGAGT GGCGCTATTT CTTTAGAAGT GAACGTTGAC GATATCAACT CCCCTATCCA TTGCTCACCG AATGGTACAG GTCGGGGACC CGAAGTTCCG ACTGTCGGCC TGATGCATCC CCGGCTGATC GACCCCAGAT CTGGGGCTGA GAAAGCCCAG TAAGGAAACA ACTGTAGGTT CGAGTCGCGA GATCCCCCGG AACCAAAGGA AGTAGGTTAA ACCCGCTCCG ATCAGGCCGA GCCACGCCAG GCCGAGAACA TTGGTTCCTG TAGGCATCGG GATTGGCGGA TCAAACACTA AAGCTACTGG AACGAGCAGA AGTCCTCCGG CCGCCAGTTG CCAGGCGGTA AAGGTGAGCA GAGGCACGGG AGGTTGCCAC TTGCGGGTCA GCACGGTTCC GAACGCCATG GAAACCGCCC CCGCCAGGCC CGCTGCGACG CCGACAGGAT CTAGCGCTGC GTTTGGTGTC AACACCAACA GCGCCACGCC CGCAGTTCCG CAAATAGCCC CCAGGACCGC CATCAATCGT ATCGGGCTAC CTAGCAGAGC GGCAGAGATG AACACGACCA TCAGCGGCTG CACAGCGCCT ACCGTCGCCG CGACCCCGCC CGGCAGGCGG TAGACCGAAA TAAACAACAA GCTCCAGAAT AGCGAAATAT TAAGTGCGCC GAGGATGAAG ATGCGCATCC ACCAGATTCC CGTTGGAATC TGTCGGACGA TCATCACGAG CAATAAACCC GCCGGCAACG CCCGCAGCAG CATACCGGCG ACCCCTCGGC CTCGCTGTTC GGGCTCCACG AAAACGCCGG ACAGATGCGC CTTGTGAGCG TCCTTGGGGC CGTCCTCCTG TTTGAAGACC GACAGCCCAA TGATCTCGCC GTCGATGTAG GCGCCGAATG CCACGGCATC TCGCAACCGT TCAGCGAACG CCTCCATGGG CTTTTTCTCC TCGTGCTCGT AAACGGACCC GAACATCTCT GGAGCTTTCT TCAGGGCCGA CAATCGGATC TCGCGGAAAT CCTGCACGTC GGCCGCTCCA AGCCGTCGAA TCTGAGCCTT AATCACAATT GTCAATTTTA ATCCTCTGTT TATCGGCAGT TCGTAGAGCG CGCCGTGCGT CCCGAGCGAT ACTGAGCGAA GCAAGTGCGT CGAGCAGTGC CCGCTTGTTC CTGAAATGCC AGTAAAGCGC TGGCTGCTGA ACCCCCAGCC GGAACTGACC CCACAAGGCC CTAGCGTTTG CAATGCACCA GGTCATCATT GACCCAGGCG TGTTCCACCA GGCCGCTGCC TCGCAACTCT TCGCAGGCTT CGCCGACCTG CTCGCGCCAC TTCTTCACGC GGGTGGAATC CGATCCGCAC ATGAGGCGGA AGGTTTCCAG CTTGAGCGGG TACGGCTCCC GGTGCGAGCT GAAATAGTCG AACATCCGTC GGGCCGTCGG CGACAGCTTG CGGTACTTCT CCCATATGAA TTTCGTGTAG TGGTCGCCAG CAAACAGCAC GACGATTTCC TCGTCGATCA GGACCTGGCA ACGGGACGTT TTCTTGCCAC GGTCCAGGAC GCGGAAGCGG TGCAGCAGCG ACACCGATTC CAGGTGCCCA ACGCGGTCGG ACGTGAAGCC CATCGCCGTC GCCTGTAGGC GCGACAGGCA TTCCTCGGCC TTCGTGTAAT ACCGGCCATT GATCGACCAG CCCAGGTCCT GGCAAAGCTC GTAGAACGTG AAGGTGATCG GCTCGCCGAT AGGGGTGCGC TTCGCGTACT CCAACACCTG CTGCCACACC AGTTCGTCAT CGTCGGCCCG CAGCTCGACG CCGGTGTAGG TGATCTTCAC GTCCTTGTTG ACGTGGAAAA TGACCTTGTT TTGCAGCGCC TCGCGCGGGA TTTTCTTGTT GCGCGTGGTG AACAGGGCAG AGCGGGCCGT GTCGTTTGGC ATCGCTCGCA TCGTGTCCGG CCACGGCGCA ATATCGAACA AGGAAAGCTG CATTTCCTTG ATCTGCTGCT TCGTGTGTTT CAGCAACGCG GCCTGCTTGG CCTCGCTGAC CTGTTTTGCC AGGTCCTCGC CGGCGGTTTT TCGCTTCTTG GTCGTCATAG TTCCTCGCGT GTCGATGGTC ATCGACTTCG CCAAACCTGC CGCCTCCTGT TCGAGACGAC GCGAACGCTC CACGGCGGCC GATGGCGCGG GCAGGGCAGG GGGAGCCAGT TGCACGCTGT CGCGCTCGAT CTTGGCCGTA GCTTGCTGGA CCATCGAGCC GACGGACTGG AAGGTTTCGC GGGGCGCACG CATGACGGTG CGGCTTGCGA TGGTTTCGGC ATCCTCGGCG GAAAACCCCG CGTCGATCAG TTCTTGCCTG TATGCCTTCC GGTCAAACGT CCGATTCATT CACCCTCCTT GCGGGATTGC CCCGACTCAC GCCGGGGCAA TGTGCCCTTA TTCCTGATTT GACCCGCCTG GTGCCTTGGT GTCCAGATAA TCCACCTTAT CGGCAATGAA GTCGGTCCCG TAGACCGTCT GGCCGTCCTT CTCGTACTTG GTATTCCGAA TCTTGCCCTG CACGAATACC AGCGACCCCT TGCCCAAATA CTTGCCGTGG GCCTCGGCCT GAGAGCCAAA ACACTTGATG CGGAAGAAGT CGGTGCGCTC CTGCTTGTCG CCGGCATCGT TGCGCCACAT CTAGGTACTA AAACAATTCA TCCAGTAAAA TATAATATTT TATTTTCTCC CAATCAGGCT TGATCCCCAG TAAGTCAAAA AATAGCTCGA CATACTGTTC TTCCCCGATA TCCTCCCTGA TCGACCGGAC GCAGAAGGCA ATGTCATACC ACTTGTCCGC CCTGCCGCTT CTCCCAAGAT CAATAAAGCC ACTTACTTTG CCATCTTTCA CAAAGATGTT GCTGTCTCCC AGGTCGCCGT GGGAAAAGAC AAGTTCCTCT TCGGGCTTTT CCGTCTTTAA AAAATCATAC AGCTCGCGCG GATCTTTAAA TGGAGTGTCT TCTTCCCAGT TTTCGCAATC CACATCGGCC AGATCGTTAT TCAGTAAGTA ATCCAATTCG GCTAAGCGGC TGTCTAAGCT ATTCGTATAG GGACAATCCG ATATGTCGAT GGAGTGAAAG AGCCTGATGC ACTCCGCATA CAGCTCGATA ATCTTTTCAG GGCTTTGTTC ATCTTCATAC TCTTCCGAGC AAAGGACGCC ATCGGCCTCA CTCATGAGCA GATTGCTCCA GCCATCATGC CGTTCAAAGT GCAGGACCTT TGGAACAGGC AGCTTTCCTT CCAGCCATAG CATCATGTCC TTTTCCCGTT CCACATCATA GGTGGTCCCT TTATACCGGC TGTCCGTCAT TTTTAAATAT AGGTTTTCAT TTTCTCCCAC CAGCTTATAT ACCTTAGCAG GAGACATTCC TTCCGTATCT TTTACGCAGC GGTATTTTTC GATCAGTTTT TTCAATTCCG GTGATATTCT CATTTTAGCC ATTTATTATT TCCTTCCTCT TTTCTACAGT ATTTAAAGAT ACCCCAAGAA GCTAATTATA ACAAGACGAA CTCCAATTCA CTGTTCCTTG CATTCTAAAA CCTTAAATAC CAGAAAACAG CTTTTTCAAA GTTGTTTTCA AAGTTGGCGT ATAACATAGT ATCGACGGAG CCGATTTTGA AACCACAATT ATGGGTGATG CTGCCAACTT ACTGATTTAG TGTATGATGG TGTTTTTGAG GTGCTCCAGT GGCTTCTGTG TCTATCAGCT GTCCCTCCTG TTCAGCTACT GACGGGGTGG TGCGTAACGG CAAAAGCACC GCCGGACATC AGCGCTATCT CTGCTCTCAC TGCCGTAAAA CATGGCAACT GCAGTTCACT TACACCGCTT CTCAACCCGG TACGCACCAG AAAATCATTG ATATGGCCAT GAATGGCGTT GGATGCCGGG CAACAGCCCG CATTATGGGC GTTGGCCTCA ACACGATTTT ACGTCACTTA AAAAACTCAG GCCGCAGTCG GTAACCTCGC GCATACAGCC GGGCAGTGAC GTCATCGTCT GCGCGGAAAT GGACGAACAG TGGGGCTATG TCGGGGCTAA ATCGCGCCAG CGCTGGCTGT TTTACGCGTA TGACAGTCTC CGGAAGACGG TTGTTGCGCA CGTATTCGGT GAACGCACTA TGGCGACGCT GGGGCGTCTT ATGAGCCTGC TGTCACCCTT TGACGTGGTG ATATGGATGA CGGATGGCTG GCCGCTGTAT GAATCCCGCC TGAAGGGAAA GCTGCACGTA ATCAGCAAGC GATATACGCA GCGAATTGAG CGGCATAACC TGAATCTGAG GCAGCACCTG GCACGGCTGG GACGGAAGTC GCTGTCGTTC TCAAAATCGG TGGAGCTGCA TGACAAAGTC ATCGGGCATT ATCTGAACAT AAAACACTAT CAATAAGTTG GAGTCATTAC CCAATTATGA TAGAATTTAC AAGCTATAAG GTTATTGTCC TGGGTTTCAA GCATTAGTCC ATGCAAGTTT TTATGCTTTG CCCATTCTAT AGATATATTG ATAAGCGCGC TGCCTATGCC TTGCCCCCTG AAATCCTTAC ATACGGCGAT ATCTTCTATA TAAAAGATAT ATTATCTTAT CAGTATTGTC AATATATTCA AGGCAATCTG CCTCCTCATC CTCTTCATCC TCTTCGTCTT GGTAGCTTTT TAAATATGGC GCTTCATAGA GTAATTCTGT AAAGGTCCAA TTCTCGTTTT CATACCTCGG TATAATCTTA CCTATCACCT CAAATGGTTC GCTGGGTTTA TCGCACCCCC GAACACGAGC ACGGCACCCG CGACCACTAT GCCAAGAATG CCCAAGGTAA AAATTGCCGG CCCCGCCATG AAGTCCGTGA ATGCCCCGAC GGCCGAAGTG AAGGGCAGGC CGCCACCCAG GCCGCCGCCC TCACTGCCCG GCACCTGGTC GCTGAATGTC GATGCCAGCA CCTGCGGCAC GTCAATGCTT CCGGGCGTCG CGCTCGGGCT GATCGCCCAT CCCGTTACTG CCCCGATCCC GGCAATGGCA AGGACTGCCA GCGCTGCCAT TTTTGGGGTG AGGCCGTTCG CGGCCGAGGG GCGCAGCCCC TGGGGGGATG GGAGGCCCGC GTTAGCGGGC CGGGAGGGTT CGAGAAGGGG GGGCACCCCC CTTCGGCGTG CGCGGTCACG CGCACAGGGC GCAGCCCTGG TTAAAAACAA GGTTTATAAA TATTGGTTTA AAAGCAGGTT AAAAGACAGG TTAGCGGTGG CCGAAAAACG GGCGGAAACC CTTGCAAATG CTGGATTTTC TGCCTGTGGA CAGCCCCTCA AATGTCAATA GGTGCGCCCC TCATCTGTCA GCACTCTGCC CCTCAAGTGT CAAGGATCGC GCCCCTCATC TGTCAGTAGT CGCGCCCCTC AAGTGTCAAT ACCGCAGGGC ACTTATCCCC AGGCTTGTCC ACATCATCTG TGGGAAACTC GCGTAAAATC AGGCGTTTTC GCCGATTTGC GAGGCTGGCC AGCTCCACGT CGCCGGCCGA AATCGAGCCT GCCCCTCATC TGTCAACGCC GCGCCGGGTG AGTCGGCCCC TCAAGTGTCA ACGTCCGCCC CTCATCTGTC AGTGAGGGCC AAGTTTTCCG CGAGGTATCC ACAACGCCGG CGGCCGCGGT GTCTCGCACA CGGCTTCGAC GGCGTTTCTG GCGCGTTTGC AGGGCCATAG ACGGCCGCCA GCCCAGCGGC GAGGGCAACC AGCCCGG
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