NM_198429.2;2210017P03Rik;AI449492;AV076380;NF-ATc;NFAT2;NFATc;Nfatcb
100μg liquid plasmid(Instantly):$120
| name | pECMV-Nfatc1-m-FLAG | another name | apECMV-Nfatc1-m-FLAG |
| Insert Size (bp) | 2484bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pFLAG-CMV-R：GCACTGGAGTGGCAACTT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGAAGTGC AGCAATAGCA GAGCTCGTTT AGTGACCGTC AGAATTTTGT AATACGACTC ACTATAGGGC GGCCGGGAAT TCGTCGACTG GATCCGGTAC CGAGGAGATC TGCCGCCGCG ATCGCCATGC CAAGTACCAG CTTTCCAGTC CCTTCCAAGT TTCCACTCGG CCCTCCGGCC GCAGTCTGCG GGAGCGGAGA AACTTTGCGG CCCGCGCCGC CCTCCGGCGG CACCATGAAG GCGGCCGAGG AAGAACACTA CAGTTATGTG TCCCCTAGTG TCACCTCGAC CCTGCCCCTT CCCACAGCAC ACTCTGCCTT GCCAGCAGCA TGCCACGACC TCCAGACGTC CACCCCGGGT ATCTCAGCTG TTCCTTCAGC CAATCATCCC CCCAGTTACG GAGGGGCTGT GGACAGCGGG CCTTCGGGAT ACTTCCTGTC CTCTGGCAAC ACCAGACCCA ACGGGGCCCC GACTCTGGAG AGTCCGAGAA TCGAGATCAC CTCCTACCTG GGCCTACACC ATGGCAGCGG CCAGTTTTTC CACGACGTGG AGGTGGAAGA CGTACTTCCT AGCTGCAAGC GCTCACCGTC TACAGCAACC CTGCACCTGC CCAGCCTGGA AGCCTACAGA GACCCCTCCT GCCTGAGCCC AGCCAGCAGT CTCTCCTCCA GAAGCTGTAA CTCTGAGGCC TCCTCCTACG AGTCCAACTA CTCCTACCCA TACGCGTCCC CCCAGACCTC TCCGTGGCAG TCACCCTGCG TGTCTCCCAA GACCACGGAC CCGGAGGAGG GTTTTCCCCG AAGCCTGGGT GCCTGCCACC TGCTAGGATC GCCCAGGCAC TCCCCATCCA CCTCTCCTCG GGCAAGCATC ACGGAGGAGA GCTGGCTCGG TGCCCGCGGC TCCCGGCCCA CGTCCCCCTG CAACAAGCGC AAGTACAGTC TCAATGGCCG GCAGCCCTCC TGCTCACCCC ACCACTCACC CACACCATCC CCCCATGGCT CCCCTCGGGT CAGTGTGACC GAAGATACCT GGCTCGGTAA CACCACCCAG TATACCAGCT CTGCCATTGT GGCAGCCATC AACGCCCTGA CCACCGATAG CACTCTGGAC CTGGGTGATG GGGTCCCTAT CAAGTCTCGA AAGACAGCAC TGGAGCATGC GCCCTCTGTG GCCCTCAAAG TAGAGCCAGC TGGGGAAGAC CTGGGCACCA CTCCACCCAC TTCTGACTTC CCACCCGAGG AGTACACCTT CCAGCACCTT CGGAAGGGTG CCTTTTGCGA GCAGTATCTG TCGGTGCCAC AGGCCTCGTA TCAGTGGGCG AAGCCCAAGT CTCTTTCCCC GACATCATAT ATGAGCCCAT CCTTGCCTGC CCTTGACTGG CAGCTCCCGT CACATTCTGG TCCATACGAG CTTCGGATCG AGGTGCAGCC CAAGTCTCAC CACAGGGCTC ACTATGAGAC GGAAGGCAGC CGGGGGGCTG TGAAGGCTTC AGCTGGAGGA CACCCCATTG TGCAGCTACA CGGTTACTTG GAGAATGAAC CTCTCACGCT ACAGCTGTTC ATTGGGACGG CTGACGACCG CCTGCTGAGG CCCCACGCCT TCTACCAGGT CCACCGGATC ACGGGGAAGA CTGTCTCCAC CACCAGCCAC GAGATCATCC TGTCCAACAC CAAAGTCCTG GAGATCCCGT TGCTTCCAGA AAATAACATG CGAGCCATCA TCGACTGTGC TGGGATCCTG AAGCTCAGAA ACTCTGATAT TGAGCTGAGG AAAGGGGAGA CAGACATCGG GAGGAAGAAC ACCAGGGTGA GGCTGGTCTT CCGAGTTCAC ATCCCACAGC CCAATGGCCG GACGCTGTCT CTCCAGGTGG CCTCGAACCC TATCGAGTGT TCCCAGCGGT CAGCCCAGGA GCTGCCCCTC GTGGAGAAGC AGAGCACAGA CAGCTACCCA GTCATCGGCG GGAAGAAGAT GGTGCTGTCT GGCCATAACT TTCTGCAAGA CTCCAAAGTC ATTTTCGTGG AGAAGGCTCC AGATGGCCAC CACGTCTGGG AGATGGAAGC AAAGACTGAC CGGGACCTGT GCAAGCCAAA TTCCCTGGTG GTTGAGATAC CACCTTTCCG CAACCAGAGG ATAACCAGCC CCGTCCAAGT CAGTTTCTAT GTCTGCAACG GGAAACGGAA GAGAAGCCAG TACCAGCGTT TCACGTACCT TCCTGCCAAT GTTCCAATTA TAAAGACAGA ACCCACGGAC GACTTTGAGC CAGCTCTGAC CTGTGGACCA ATGAGCCAGG GGATTAGTCC TCTGCCGAGG CCTTACTACA GCCAACAGCT CACCATGCCT CCCGACCCCG GCTCCTGCCT CGTGGCTGGC TTCGCCCCCT GCTCCCAGAG GAACACGCTG ATGCCCACGC CTCCCAACGC AAGCCCGAAG CTCCACGACC TTTCCTCTCC TGCCTACACC AAGGGCCTCA CCAACCCGGG CCACAGTGGT CACCTTGGAC TTCAGCCACC CGCTTCGGAG GCCCCCACCA TGCAGGAAGT GCCGAGACCC ATGGCCATCC AACCCAACTC GCCTGAGCAG CCCCCATCCG CCAGGCTACA GCCGCAGAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGT GGAAAGGGGG GGGGGTATGG G
