pCMV-FLAG-KIF14
$128.00
In stock
SKU
PL78468
NM_014875.2;MCPH20;MKS12
100μg liquid plasmid(Instantly):$149
| name | pCMV-FLAG-KIF14 | another name | apCMV-FLAG-KIF14 |
| Insert Size (bp) | 4947bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCAGGCC CGAATTCGGT CGACGCCACC ATGTCATTAC ACAGTACTCA TAATAGAAAT AACAGCGGTG ATATTCTTGA TATTCCTTCT TCCCAAAATA GTTCATCACT GAATGCCCTC ACCCACAGTA GCCGACTTAA GCTGCATTTG AAGTCGGATA TGTCAGAATG TGAAAATGAT GATCCATTAT TGAGATCTGC AGGTAAAGTC AGAGACATAA ATAGAACTTA TGTTATTTCT GCCAGTAGAA AAACAGCAGA CATGCCCCTT ACCCCTAATC CTGTAGGTAG ATTGGCACTT CAGAGGAGAA CTACAAGGAA CAAAGAATCA TCTTTGCTTG TTAGTGAGTT GGAAGACACA ACTGAAAAAA CAGCAGAAAC ACGTCTTACA TTACAACGTC GTGCTAAAAC AGATTCTGCA GAAAAGTGGA AAACAGCTGA AATAGATTCT GTCAAAATGA CACTGAATGT GGGAGGTGAA ACAGAAAATA ATGGTGTTTC TAAGGAAAGT AGAACAAATG TAAGGATTGT AAATAATGCT AAAAACTCTT TTGTTGCCTC TTCTGTACCT TTAGATGAAG ATCCACAGGT CATTGAAATG ATGGCTGATA AGAAATACAA AGAAACATTT TCTGCCCCCA GTAGAGCAAA TGAAAATGTT GCACTTAAGT ACTCAAGTAA TAGACCACCC ATTGCTTCCC TGAGTCAGAC TGAAGTTGTT AGATCAGGAC ACTTGACAAC GAAACCTACT CAGAGCAAGT TGGATATCAA AGTGTTGGGA ACAGGAAACT TGTATCATAG AAGTATTGGG AAGGAAATTG CAAAAACTTC AAATAAATTT GGGAGCTTAG AAAAAAGAAC ACCTACAAAA TGTACAACAG AACACAAACT GACAACAAAG TGCAGCCTGC CTCAGCTTAA GAGCCCAGCT CCATCAATAC TGAAGAATAG AATGTCTAAC CTTCAAGTTA AACAAAGACC AAAAAGTTCC TTTCTTGCAA ATAAACAGGA AAGATCCGCA GAAAATACAA TTCTTCCCGA AGAAGAAACT GTAGTTCAGA ACACCTCTGC AGGAAAAGAC CCCTTAAAAG TAGAGAATAG TCAAGTGACA GTGGCAGTAC GCGTAAGACC TTTCACCAAG AGAGAGAAGA TTGAAAAAGC ATCCCAGGTA GTCTTCATGA GTGGGAAAGA AATAACTGTG GAACACCCTG ACACGAAACA AGTTTATAAT TTTATTTATG ATGTTTCATT CTGGTCTTTT GATGAATGTC ATCCTCACTA CGCTAGCCAG ACAACTGTCT ATGAGAAGCT AGCAGCACCA CTCCTAGAAA GAGCCTTCGA AGGCTTCAAT ACCTGTCTTT TTGCTTATGG TCAGACTGGC TCTGGAAAAT CATATACGAT GATGGGATTT AGTGAAGAAC CAGGAATAAT TCCAAGATTT TGTGAAGATC TTTTTTCTCA AGTAGCCAGA AAACAAACCC AAGAGGTCAG CTATCACATT GAAATGAGCT TCTTTGAAGT ATATAATGAA AAAATTCACG ACCTTCTGGT TTGTAAAGAT GAAAATGGGC AGAGAAAGCA ACCACTGAGA GTGAGGGAAC ATCCTGTTTA TGGACCATAT GTTGAAGCAC TGTCAATGAA CATTGTCAGT TCTTACGCTG ATATCCAGAG TTGGCTAGAA TTGGGAAATA AACAAAGAGC TACTGCTGCT ACTGGTATGA ATGATAAAAG TTCCCGATCT CATTCAGTTT TCACCCTGGT GATGACCCAG ACCAAGACAG AATTTGTGGA AGGGGAAGAA CACGATCACA GAATAACAAG TCGAATTAAC CTAATAGATC TGGCAGGCAG TGAGCGCTGC TCTACGGCTC ACACTAATGG AGATCGACTA AAGGAAGGTG TGAGTATTAA TAAGTCCTTG CTAACTTTGG GAAAAGTTAT ATCTGCACTT TCGGAACAAG CAAACCAAAG GAGTGTTTTT ATTCCTTATC GTGAATCTGT TCTTACATGG CTGTTAAAAG AAAGTCTGGG TGGAAATTCA AAAACTGCAA TGATTGCTAC GATTAGTCCC GCTGCCAGCA ACATAGAAGA AACATTAAGC ACACTTAGAT ATGCTAACCA AGCCCGTTTA ATAGTCAACA TTGCTAAAGT AAATGAAGAT ATGAACGCTA AGTTAATTAG AGAATTGAAG GCAGAAATTG CAAAGCTAAA AGCTGCTCAG AGAAACAGTC GGAATATTGA CCCTGAACGA TACAGGCTCT GTCGGCAAGA AATAACATCC TTAAGAATGA AACTGCATCA ACAGGAGAGA GACATGGCAG AAATGCAAAG AGTGTGGAAA GAAAAGTTTG AACAAGCTGA AAAAAGAAAA CTTCAAGAAA CAAAAGAGTT ACAGAAAGCA GGAATTATGT TTCAAATGGA CAATCATTTA CCAAACCTTG TTAATCTGAA TGAAGATCCA CAACTATCTG AGATGCTGCT ATATATGATA AAAGAAGGAA CAACTACAGT TGGAAAGTAT AAACCAAACT CAAGCCATGA TATTCAGTTA TCTGGGGTGC TGATTGCTGA TGATCATTGT ACTATCAAAA ATTTTGGTGG GACAGTGAGT ATTATCCCAG TTGGGGAAGC AAAGACATAT GTAAATGGAA AACATATTTT GGAAATCACA GTATTACGTC ATGGTGATCG AGTGATTCTT GGTGGAGATC ATTATTTTAG ATTTAATCAT CCAGTAGAAG TCCAGAAAGG AAAAAGGCCA TCTGGAAGAG ATACTCCTAT AAGTGAGGGT CCAAAAGACT TTGAATTTGC AAAAAATGAG TTGCTCATGG CACAGAGATC ACAACTTGAA GCAGAAATAA AAGAGGCTCA GTTGAAGGCA AAGGAAGAAA TGATGCAAGG AATCCAGATT GCAAAAGAAA TGGCTCAGCA AGAGCTTTCT TCTCAAAAAG CTGCATATGA AAGCAAAATA AAAGCACTGG AAGCAGAACT GAGAGAAGAG TCTCAAAGGA AAAAAATGCA GGAAATAAAT AACCAGAAGG CTAATCACAA AATTGAGGAA TTAGAAAAGG CAAAGCAGCA TCTTGAACAG GAAATATATG TCAACAAAAA GCGATTAGAA ATGGAAACAT TGGCTACAAA ACAGGCTTTA GAAGACCATA GCATCCGCCA TGCAAGAATT CTGGAAGCTT TAGAAACTGA AAAGCAAAAA ATTGCTAAAG AAGTACAAAT TCTACAGCAG AATCGGAATA ATAGGGATAA AACTTTTACA GTGCAGACAA CTTGGAGCTC TATGAAACTC TCAATGATGA TTCAGGAAGC CAATGCTATC AGCAGCAAAT TGAAAACATA CTATGTTTTT GGCAGACATG ATATATCAGA TAAAAGTAGT TCTGACACTT CTATTCGGGT TCGTAACCTG AAACTAGGAA TCTCAACATT CTGGAGTCTG GAAAAGTTTG AATCTAAACT TGCAGCAATG AAAGAACTTT ATGAGAGTAA TGGTAGTAAC AGGGGTGAAG ATGCCTTTTG TGATCCTGAA GATGAATGGG AACCCGACAT TACAGATGCA CCAGTTTCTT CACTTTCTAG AAGGAGGAGT AGGAGTTTGA TGAAGAACAG AAGAATTTCT GGTTGTTTAC ATGACATACA AGTCCATCCA ATTAAGAATT TGCATTCTTC ACATTCATCA GGTTTAATGG ACAAATCAAG CACTATTTAC TCAAATTCAG CAGAGTCCTT TCTTCCTGGA ATTTGCAAAG AATTGATTGG TTCTTCGTTA GATTTTTTTG GACAGAGTTA TGATGAAGAA AGAACTATAG CAGACAGCCT AATTAATAGT TTTCTTAAAA TTTATAATGG GCTATTTGCC ATTTCCAAGG CTCATGAAGA ACAAGATGAA GAAAGTCAAG ATAACTTGTT TTCTTCTGAT CGAGCAATCC AGTCACTTAC TATTCAGACT GCATGTGCTT TTGAGCAGCT AGTAGTGCTA ATGAAACACT GGCTGAGTGA TTTACTGCCT TGTACCAACA TAGCAAGACT TGAGGATGAG TTGAGACAAG AAGTTAAAAA ACTGGGAGGC TACTTACAGT TATTTTTGCA GGGATGCTGT TTGGATATTT CATCAATGAT AAAAGAGGCT CAAAAGAATG CAATCCAAAT TGTACAACAA GCTGTAAAGT ATGTGGGGCA GTTAGCAGTT CTGAAAGGGA GCAAGCTACA TTTTCTAGAA AACGGTAACA ATAAAGCTGC CAGTGTCCAG GAGGAATTCA TGGATGCTGT TTGTGATGGT GTAGGCTTAG GAATGAAGAT TTTATTAGAT TCTGGACTGG AAAAAGCAAA AGAACTTCAG CATGAACTCT TTAGGCAGTG TACAAAAAAT GAGGTTACCA AAGAAATGAA AACTAATGCC ATGGGATTGA TTAGATCTCT TGAAAACATC TTTGCTGAAT CGAAAATTAA AAGTTTCAGA AGGCAAGTAC AAGAAGAAAA CTTTGAATAC CAAGATTTCA AGAGGATGGT TAATCGTGCT CCAGAATTCT TAAAGTTAAA ACATTGCTTA GAGAAAGCTA TTGAAATTAT TATTTCTGCA CTGAAAGGAT GCCATAGTGA TATAAATCTT CTCCAGACTT GTGTTGAAAG TATTCGCAAC TTGGCCAGTG ATTTTTACAG TGACTTCAGT GTGCCTTCTA CTTCTGTTGG CAGCTATGAG AGTAGAGTAA CTCACATTGT CCACCAGGAA CTAGAATCTC TAGCTAAGTC TCTCCTCTTT TGTTTTGAAT CTGAAGAAAG CCCTGATTTG TTGAAACCCT GGGAAACTTA TAATCAAAAT ACCAAAGAAG AACACCAACA ATCTAAATCA AGCGGGATTG ACGGCAGTAA GAATAAAGGT GTACCAAAGC GTGTCTATGA GCTCCATGGC TCATCCCCAG CAGTGAGCTC AGAGGAATGC ACACCCAGTA GGATTCAGTG GGTGTCTCGA GGTACCGGCG ACTACAAGGA CGACGATGAC AAGTAAGCGG CCGCGGGGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTCGGATCC TCTAGAGTCG ATCTGCAGGC ATGCTAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGT
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