pLP-VSVG
$55.00
In stock
SKU
PL78182
DB00033;pLPVSVG;pLP/VSVG
100μg liquid plasmid(Instantly):$57
| name | pLP-VSVG | another name | apLP-VSVG |
| Insert Size (bp) | Plasmid host | Mammalian cell,Lentivirus | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Forward(left ITR)：CCTCTGACTTGAGCGTCGAT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCCCT CGAAGCTTAC ATGTGGTACC GAGCTCGGAT CCTGAGAACT TCAGGGTGAG TCTATGGGAC CCTTGATGTT TTCTTTCCCC TTCTTTTCTA TGGTTAAGTT CATGTCATAG GAAGGGGAGA AGTAACAGGG TACACATATT GACCAAATCA GGGTAATTTT GCATTTGTAA TTTTAAAAAA TGCTTTCTTC TTTTAATATA CTTTTTTGTT TATCTTATTT CTAATACTTT CCCTAATCTC TTTCTTTCAG GGCAATAATG ATACAATGTA TCATGCCTCT TTGCACCATT CTAAAGAATA ACAGTGATAA TTTCTGGGTT AAGGCAATAG CAATATTTCT GCATATAAAT ATTTCTGCAT ATAAATTGTA ACTGATGTAA GAGGTTTCAT ATTGCTAATA GCAGCTACAA TCCAGCTACC ATTCTGCTTT TATTTTATGG TTGGGATAAG GCTGGATTAT TCTGAGTCCA AGCTAGGCCC TTTTGCTAAT CATGTTCATA CCTCTTATCT TCCTCCCACA GCTCCTGGGC AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG CACGTGAGAT CTGAATTCTG ACACTATGAA GTGCCTTTTG TACTTAGCCT TTTTATTCAT TGGGGTGAAT TGCAAGTTCA CCATAGTTTT TCCACACAAC CAAAAAGGAA ACTGGAAAAA TGTTCCTTCT AATTACCATT ATTGCCCGTC AAGCTCAGAT TTAAATTGGC ATAATGACTT AATAGGCACA GCCTTACAAG TCAAAATGCC CAAGAGTCAC AAGGCTATTC AAGCAGACGG TTGGATGTGT CATGCTTCCA AATGGGTCAC TACTTGTGAT TTCCGCTGGT ATGGACCGAA GTATATAACA CATTCCATCC GATCCTTCAC TCCATCTGTA GAACAATGCA AGGAAAGCAT TGAACAAACG AAACAAGGAA CTTGGCTGAA TCCAGGCTTC CCTCCTCAAA GTTGTGGATA TGCAACTGTG ACGGATGCCG AAGCAGTGAT TGTCCAGGTG ACTCCTCACC ATGTGCTGGT TGATGAATAC ACAGGAGAAT GGGTTGATTC ACAGTTCATC AACGGAAAAT GCAGCAATTA CATATGCCCC ACTGTCCATA ACTCTACAAC CTGGCATTCT GACTATAAGG TCAAAGGGCT ATGTGATTCT AACCTCATTT CCATGGACAT CACCTTCTTC TCAGAGGACG GAGAGCTATC ATCCCTGGGA AAGGAGGGCA CAGGGTTCAG AAGTAACTAC TTTGCTTATG AAACTGGAGG CAAGGCCTGC AAAATGCAAT ACTGCAAGCA TTGGGGAGTC AGACTCCCAT CAGGTGTCTG GTTCGAGATG GCTGATAAGG ATCTCTTTGC TGCAGCCAGA TTCCCTGAAT GCCCAGAAGG GTCAAGTATC TCTGCTCCAT CTCAGACCTC AGTGGATGTA AGTCTAATTC AGGACGTTGA GAGGATCTTG GATTATTCCC TCTGCCAAGA AACCTGGAGC AAAATCAGAG CGGGTCTTCC AATCTCTCCA GTGGATCTCA GCTATCTTGC TCCTAAAAAC CCAGGAACCG GTCCTGCTTT CACCATAATC AATGGTACCC TAAAATACTT TGAGACCAGA TACATCAGAG TCGATATTGC TGCTCCAATC CTCTCAAGAA TGGTCGGAAT GATCAGTGGA ACTACCACAG AAAGGGAACT GTGGGATGAC TGGGCACCAT ATGAAGACGT GGAAATTGGA CCCAATGGAG TTCTGAGGAC CAGTTCAGGA TATAAGTTTC CTTTATACAT GATTGGACAT GGTATGTTGG ACTCCGATCT TCATCTTAGC TCAAAGGCTC AGGTGTTCGA ACATCCTCAC ATTCAAGACG CTGCTTCGCA ACTTCCTGAT GATGAGAGTT TATTTTTTGG TGATACTGGG CTATCCAAAA ATCCAATCGA GCTTGTAGAA GGTTGGTTCA GTAGTTGGAA AAGCTCTATT GCCTCTTTTT TCTTTATCAT AGGGTTAATC ATTGGACTAT TCTTGGTTCT CCGAGTTGGT ATCCATCTTT GCATTAAATT AAAGCACACC AAGAAAAGAC AGATTTATAC AGACATAGAG ATGAACCGAC TTGGAAAGTA ACTCAAATCC TGCACAACAG ATTCTTCATG TTTGGACCAA ATCAACTTGT GATACCATGC TCAAAGAGGC CTCAATTATA TTTGAGTTTT TAATTTTTAT GAAAAAAAAA AAAAAAAACG GAATTCACCC CACCAGTGCA GGCTGCCTAT CAGAAAGTGG TGGCTGGTGT GGCTAATGCC CTGGCCCACA AGTATCACTA AGCTCGCTTT CTTGCTGTCC AATTTCTATT AAAGGTTCCT TTGTTCCCTA AGTCCAACTA CTAAACTGGG GGATATTATG AAGGGCCTTG AGCATCTGGA TTCTGCCTAA TAAAAAACAT TTATTTTCAT TGCAATGATG TATTTAAATT ATTTCTGAAT ATTTTACTAA AAAGGGAATG TGGGAGGTCA GTGCATTTAA AACATAAAGA AATGAAGAGC TAGTTCAAAC CTTGGGAAAA TACACTATAT CTTAAACTCC ATGAAAGAAG GTGAGGCTGC AAACAGCTAA TGCACATTGG CAACAGCCCC TGATGCCTAT GCCTTATTCA TCCCTCAGAA AAGGATTCAA GTAGAGGCTT GATTTGGAGG TTAAAGTTTT GCTATGCTGT ATTTTACATT ACTTATTGTT TTAGCTGTCC TCATGAATGT CTTTTCACTA CCCATTTGCT TATCCTGCAT CTCTCAGCCT TGACTCCACT CAGTTCTCTT GCTTAGAGAT ACCACCTTTC CCCTGAAGTG TTCCTTCCAT GTTTTACGGC GAGATGGTTT CTCCTCGCCT GGCCACTCAG CCTTAGTTGT CTCTGTTGTC TTATAGAGGT CTACTTGAAG AAGGAAAAAC AGGGGGCATG GTTTGACTGT CCTGTGAGCC CTTCTTCCCT GCCTCCCCCA CTCACAGTGA CCCGGAATCC CTCGACATGG CAGTCTAGCA CTAGTGCGGC CGCAGATCTG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGG GATCCCCTGA GGGGGCCCCC ATGGGCTAGA GGATCCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGAG C
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