pDM4
$157.00
In stock
SKU
PL77658
100μg liquid plasmid(Instantly):$192
| name | pDM4 | another name | apDM4 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | R6K | |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | P0103-F：gtgacaatcacgaaacgcgg | |
| 3′ sequencing primer | P0103-R：ggtatttattcggcgcaaagtgc | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATCCTTTTT GTCCGGTGTT GGGTTGAAGG TGAAGCCGGT CGGGGCCGCA GCGGGGGCCG GCTTTTCAGC CTTGCCCCCC TGCTTCGGCC GCCGTGGCTC CGGCGTCTTG GGTGCCGGCG CGGGTTCCGC AGCCTTGGCC TGCGGTGCGG GCACATCGGC GGGCTTGGCC TTGATGTGCC GCCTGGCGTG CGAGCGGAAC GTCTCGTAGG AGAACTTGAC CTTCCCCGTT TCCCGCATGT GCTCCCAAAT GGTGACGAGC GCATAGCCGG ACGCTAACGC CGCCTCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTGG GTCACGTGCG CGTCACGAAC TGCGAGGCAA ACACCCTGCC CGCTGTCATG GCCGAGGTGA TGGCGACCCA GCACGGCAAC ACCCGTTCCG AGGCCGACAA GACCTATCAC CTGCTGGTTA GCTTCCGCGC GGGAGAGAAG CCCGACGCGG AGACGTTGCG CGCGATTGAG GACCGCATCT GCGCTGGGCT TGGCTTCGCC GAGCATCAGC GCGTCAGTGC CGTGCATCAC GACACCGACA ACCTGCACAT CCATATCGCC ATCAACAAGA TTCACCCGAC CCGAAACACC ATCCATGAGC CGTATCGGGC CTACCGCGCC CTCGCTGACC TCTGCGCGAC GCTCGAACGG GACTACGGGC TTGAGCGTGA CAATCACGAA ACGCGGCAGC GCGTTTCCGA GAACCGCGCG AACGACATGG AGCGGCACGC GGGCGTGGAA AGCCTGGTCG GCTGGATCCG GCCACGATGC GTCCGGCGTA GAGGATCTGA AGATCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTGAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CCACATGTGG AATTCCACAT GTGGAATTGT GAGCGGATAA CAATTTGTGG AATCCCGGGA GAGCTCAGGT TACCCGCATG CAAGATCTAT CTAGAAGGGC CCCACTAGTG ACGCGTACTC GAGGGTCGAC GGTATCGATA AGCTTGATAT ACACTCCGCT AGCGCTGATG TCCGGCGGTG CTTTTGCCGT TACGCACCAC CCCGTCAGTA GCTGAACAGG AGGGACAGCT GATAGAAACA GAAGCCACTG GAGCACCTCA AAAACACCAT CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAArGAA GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT ATTGAGAATA TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT TTGTGATGGC TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG GCAGGGCGGG GCGTAATTTT TTTAAGGCAG TTATTGGTGC CCTTAAACGC CTGGTTGCTA CGCCTGAATA AGTGATAATA AGCGGATGAA TGGCAGAAAT TCGAAAGCAA ATTCGACCCG GTCGTCGGTT CAGGGCAGGG TCGTTAAATA GCCGCTTATG TCTATTGCTG GTTTACCGGT TTATTGACTA CCGGAAGCAG TGTGACCGTG TGCTTCTCAA ATGCCTGAGG CCAGwTTGCT CAGCTCTCCC GTGGAGGTAA TAATTGACGA TATGATCATT TATTCTGCCT CCCAGAGCCT GATAAAAACG GTTAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCAT CGAATTCCTG CAGCCAAGCT AGACCTAGGC CTTAAGATCC TTTTTAACCC ATCACATATA CCTGCCGTTC ACTATTATTT AGTGAAATGA GATATTATGA TATTTTCTGA ATTGTGATTA AAAAGGCAAC TTTATGCCCA TGCAACAGAA ACTATAAAAA ATACAGAGAA TGAAAAGAAA CAGATAGATT TTTTAGTTCT TTAGGCCCGT AGTCTGCAAA TCCTTTTATG ATTTTCTATC AAACAAAAGA GGAAAATAGA CCAGTTGCAA TCCAAACGAG AGTCTAATAG AATGAGGTCG AAAAGTAAAT CGCGCGGGTT TGTTACTGAT AAAGCAGGCA AGACCTAAAA TGTGTAAAGG GCAAAGTGTA TACTTTGGCG TCACCCCTTA CATATTTTAG GTCTTTTTTT ATTGTGCGTA ACTAACTTGC CATCTTCAAA CAGGAGGGCT GGAAGAAGCA GACCGCTAAC ACAGTACATA AAAAAGGAGA CATGAACGAT GAACATCAAA AAGTTTGCAA AACAAGCAAC AGTATTAACC TTTACTACCG CACTGCTGGC AGGAGGCGCA ACTCAAGCGT TTGCGAAAGA AACGAACCAA AAGCCATATA AGGAAACATA CGGCATTTCC CATATTACAC GCCATGATAT GCTGCAAATC CCTGAACAGC AAAAAAATGA AAAATATCAA GTTCCTGAAT TCGATTCGTC CACAATTAAA AATATCTCTT CTGCAAAAGG CCTGGACGTT TGGGACAGCT GGCCATTACA AAACGCTGAC GGCACTGTCG CAAACTATCA CGGCTACCAC ATCGTCTTTG CATTAGCCGG AGATCCTAAA AATGCGGATG ACACATCGAT TTACATGTTC TATCAAAAAG TCGGCGAAAC TTCTATTGAC AGCTGGAAAA ACGCTGGCCG CGTCTTTAAA GACAGCGACA AATTCGATGC AAATGATTCT ATCCTAAAAG ACCAAACACA AGAATGGTCA GGTTCAGCCA CATTTACATC TGACGGAAAA ATCCGTTTAT TCTACACTGA TTTCTCCGGT AAACATTACG GCAAACAAAC ACTGACAACT GCACAAGTTA ACGTATCAGC ATCAGACAGC TCTTTGAACA TCAACGGTGT AGAGGATTAT AAATCAATCT TTGACGGTGA CGGAAAAACG TATCAAAATG TACAGCAGTT CATCGATGAA GGCAACTACA GCTCAGGCGA CAACCATACG CTGAGAGATC CTCACTACGT AGAAGATAAA GGCCACAAAT ACTTAGTATT TGAAGCAAAC ACTGGAACTG AAGATGGCTA CCAAGGCGAA GAATCTTTAT TTAACAAAGC ATACTATGGC AAAAGCACAT CATTCTTCCG TCAAGAAAGT CAAAAACTTC TGCAAAGCGA TAAAAAACGC ACGGCTGAGT TAGCAAACGG CGCTCTCGGT ATGATTGAGC TAAACGATGA TTACACACTG AAAAAAGTGA TGAAACCGCT GATTGCATCT AACACAGTAA CAGATGAAAT TGAACGCGCG AACGTCTTTA AAATGAACGG CAAATGGTAC CTGTTCACTG ACTCCCGCGG ATCAAAAATG ACGATTGACG GCATTACGTC TAACGATATT TACATGCTTG GTTATGTTTC TAATTCTTTA ACTGGCCCAT ACAAGCCGCT GAACAAAACT GGCCTTGTGT TAAAAATGGA TCTTGATCCT AACGATGTAA CCTTTACTTA CTCACACTTC GCTGTACCTC AAGCGAAAGG AAACAATGTC GTGATTACAA GCTATATGAC AAACAGAGGA TTCTACGCAG ACAAACAATC AACGTTTGCG CCAAGCTTCC TGCTGAACAT CAAAGGCAAG AAAACATCTG TTGTCAAAGA CAGCATCCTT GAACAAGGAC AATTAACAGT TAACAAATAA AAACGCAAAA GAAAATGCCG ATATCCTATT GGCATTTTCT TTTATTTCTT ATCAACATAA AGGTGAATCC CATATGAACT ATATAAAAGC AGGCAAATGG CTAACCGTAT TCCTAACCTT TTGGTAATGA CTCCAACTTA TTGATAGTGT TTTATGTTCA GATAATGCCC GATGACTTTG TCATGCAGCT CCACCGATTT TGAGAACGAC AGCGACTTCC GTCCCAGCCG TGCCAGGTGC TGCCTCAGAT TCAGGTTATG CCGCTCAATT CGCTGCGTAT ATCGCTTGCT GATTACGTGC AGCTTTCCCT TCAGGCGGGA TTCATACAGC GGCCAGCCAT CCGTCATCCA TATCACCACG TCAAAGGGTG ACAGCAGGCT CATAAGACGC CCCAGCGTCG CCATAGTGCG TTCACCGAAT ACGTGCGCAA CAACCGTCTT CCGGAGACTG TCATACGCGT AAAACAGCCA GCGCTGGCGC GATTTAGCCC CGACATAGCC CCACTGTTCG TCCATTTCCG CGCAGACGAT GACGTCACTG CCCGGCTGTA TGCGCGAGGT TACCGACTGC GGCCTGAGTT TTTTAAGTGA CGTAAAATCG TGTTGAGGCC AACGCCCATA ATGCGGGCTG TTGCCCGGCA TCCAACGCCA TTCATGGCCA TATCAATGAT TTTCTGGTGC GTACCGGGTT GAGAAGCGGT GTAAGTGAAC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAG
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