pCP20
$128.00
In stock
SKU
PL77608
100μg liquid plasmid(Instantly):$163
| name | pCP20 | another name | apCP20 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | Prokaryotic resistance | Amp,Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pSC101 | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | pCP-F：TCCCAGCCCTGTGTATAAC | |
| 3′ sequencing primer | pCP-R：GGAAAGTTATCGCTAGTCAGTG | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGACACAAC GTGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG GGGCGATTCA GGCCTGGTAT GAGTCAGCAA CACCTTCTTC ACGAGGCAGA CCTCAGCGCC ACAGGTGCGG TTGCTGGCGC TAACCGTTTT TATCAGGCTC TGGGAGGCAG AATAAATGAT CATATCGTCA ATTATTACCT CCACGGGGAG AGCCTGAGCA AACTGGCCTC AGGCATTTGA GAAGCACACG GTCACACTGC TTCCGGTAGT CAATAAACCG GTAAACCAGC AATAGACATA AGCGGCTATT TAACGACCCT GCCCTGAACC GACGACCGGG TCGAATTTGC TTTCGAATTT CTGCCATTCA TCCGCTTATT ATCACTTATT CAGGCGTAGC AACCAGGCGT TTAAGGGCAC CAATAACTGC CTTAAAAAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC ATTTTCGCCA AAAGTTGGCC CAGGGCTTCC CGGTATCAAC AGGGACACCA GGATTTATTT ATTCTGCGAA GTGATCTTCC GTCACAGGTA TTTATTCGGC GCAAAGTGCG TCGGGTGATG CTGCCAACTT ACTGATTTAG TGTATGATGG TGTTTTTGAG GTGCTCCAGT GGCTTCTGTT TCTATCAGCT GTCCCTCCTG TTCAGCTACT GACGGGGTGG TGCGTAACGG CAAAAGCACC GCCGGACATC AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC TGTAGTGCCA TTTACCCCCA TTCACTGCCA GAGCCGTGAG CGCAGCGAAC TGAATGTCAC GAAAAAGACA GCGACTCAGG TGCCTGATGG TCGGAGACAA AAGGAATATT CAGCGATTTG CCCGAGCTTG CGAGGGTGCT ACTTAAGCCT TTAGGGTTTT AAGGTCTGTT TTGTAGAGGA GCAAACAGCG TTTGCGACAT CCTTTTGTAA TACTGCGGAA CTGACTAAAG TAGTGAGTTA TACACAGGGC TGGGATCTAT TCTTTTTATC TTTTTTTATT CTTTCTTTAT TCTATAAATT ATAACCACTT GAATATAAAC AAAAAAAACA CACAAAGGTC TAGCGGAATT TACAGAGGGT CTAGCAGAAT TTACAAGTTT TCCAGCAAAG GTCTAGCAGA ATTTACAGAT ACCCACAACT CAAAGGAAAA GGACTAGTAA TTATCATTGA CTAGCCCATC TCAATTGGTA TAGTGATTAA AATCACCTAG ACCAATTGAG ATGTATGTCT GAATTAGTTG TTTTCAAAGC AAATGAACTA GCGATTAGTC GCTATGACTT AACGGAGCAT GAAACCAAGC TAATTTTATG CTGTGTGGCA CTACTCAACC CCACGATTGA AAACCCTACA AGGAAAGAAC GGACGGTATC GTTCACTTAT AACCAATACG TTCAGATGAT GAACATCAGT AGGGAAAATG CTTATGGTGT ATTAGCTAAA GCAACCAGAG AGCTGATGAC GAGAACTGTG GAAATCAGGA ATCCTTTGGT TAAAGGCTTT GAGATTTTCC AGTGGACAAA CTATGCCAAG TTCTCAAGCG AAAAATTAGA ATTAGTTTTT AGTGAAGAGA TATTGCCTTA TCTTTTCCAG TTAAAAAAAT TCATAAAATA TAATCTGGAA CATGTTAAGT CTTTTGAAAA CAAATACTCT ATGAGGATTT ATGAGTGGTT ATTAAAAGAA CTAACACAAA AGAAAACTCA CAAGGCAAAT ATAGAGATTA GCCTTGATGA ATTTAAGTTC ATGTTAATGC TTGAAAATAA CTACCATGAG TTTAAAAGGC TTAACCAATG GGTTTTGAAA CCAATAAGTA AAGATTTAAA CACTTACAGC AATATGAAAT TGGTGGTTGA TAAGCGAGGC CGCCCGACTG ATACGTTGAT TTTCCAAGTT GAACTAGATA GACAAATGGA TCTCGTAACC GAACTTGAGA ACAACCAGAT AAAAATGAAT GGTGACAAAA TACCAACAAC CATTACATCA GATTCCTACC TACATAACGG ACTAAGAAAA ACACTACACG ATGCTTTAAC TGCAAAAATT CAGCTCACCA GTTTTGAGGC AAAATTTTTG AGTGACATGC AAAGTAAGTA TGATCTCAAT GGTTCGTTCT CATGGCTCAC GCAAAAACAA CGAACCACAC TAGAGAACAT ACTGGCTAAA TACGGAAGGA TCTGAGGTTC TTATGGCTCT TGTATCTATC AGTGAAGCAT CAAGACTAAC AAACAAAAGT AGAACAACTG TTCACCGTTA CATATCAAAG GGAAAACTGT CCATATGCAC AGATGAAAAC GGTGTAAAAA AGATAGATAC ATCAGAGCTT TTACGAGTTT TTGGTGCATT TAAAGCTGTT CACCATGAAC AGATCGACAA TGTAACAGAT GAACAGCATG TAACACCTAA TAGAACAGGT GAAACCAGTA AAACAAAGCA ACTAGAACAT GAAATTGAAC ACCTGAGACA ACTTGTTACA GCTCAACAGT CACACATAGA CAGCCTGAAA CAGGCGATGC TGCTTATCGA ATCAAAGCTG CCGACAACAC GGGAGCCAGT GACGCCTCCC GTGGGGAAAA AATCATGGCA ATTCTGGAAG AAATAGCGCC TGTTTCGTTT CAGGCAGGTT ATCAGGGAGT GTCAGCGTCC TGCGGTTCTC CGGGGCGTTC GGGTCATGCA GCCCGTAATG GTGATTTACC AGCGTCTGCC AGGCATCAAT TCTAGGCCTG TCTGCGCGGT CGTAGTACGG CTGGAGGCGT TTTCCGGTCT GTAGCTCCAT GTTCGGAATG ACAAAATTCA GCTCAAGCCG TCCCTTGTCC TGGTGCTCCA CCCACAGGAT GCTGTACTGA TTTTTTTCGA GACCGGGCAT CAGTACACGC TCAAAGCTCG CCATCACTTT TTCACGTCCT CCCGGCGGCA GCTCCTTCTC CGCGAACGAC AGAACACCGG ACGTGTATTT CTTCGCAAAT GGCGTGGCAT CGATGAGTTC CCGGACTTCT TCCGGATTAC CCTGAAGCAC CGTTGCGCCT TCGCGGTTAC GCTCCCTCCC CAGCAGGTAA TCAACCGGAC CACTGCCACC ACCTTTTCCC CTGGCATGAA ATTTAACTAT CATCCCGCGC CCCCTGTTCC CTGACAGCCA GACGCAGCCG GCGCAGCTCA TCCCCGATGG CCATCAGTGC GGCCACCACC TGAACCCGGT CACCGGAAGA CCACTGCCCG CTGTTCACCT TACGGGCTGT CTGATTCAGG TTATTTCCGA TGGCGGCCAG CTGACGCAGT AACGGCGGTG CCAGTGTCGG CAGTTTTCCG GAACGGGCAA CCGGCTCCCC CAGGCAGACC CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAGCGTT CAAGTAACCG GGCATGTTCA TCATCAGTAA CCCGTATTGT GAGCATCCTC TCGCGTTTCA TCGGTATCAT TACCCCATGA ACAGAAATCC CCCTTACACG GAGGCATCAG TGACTAAACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAATTTTA TAAACCGTGG AGCGGGCAAT ACTGAGCTGA TGAGCAATTT CCGTTGCACC AGTGCCCTTC TGATGAAGCG TCAGCACGAC GTTCCTGTCC ACGGTACGCC TGCGGCCAAA TTTGATTCCT TTCAGCTTTG CTTCCTGTCG GCCCTCATTC GTGCGCTCTA GGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCTGAGGTC TGCCTCGTGA AGAAGGTGTT GCTGACTCAT ACCAGGCCTG AATCGCCCCA TCATCCAGCC AGAAAGTGAG GGAGCCACGG TTGATGAGAG CTTTGTTGTA GGTGGACCAG TTGGTGATTT TGAACTTTTG CTTTGCCACG GAACGGTCTG CGTTGTCGGG AAGATGCGTG ATCTGATCCT TCAACTCAGC AAAAGTTCGA TTTATTCAAC AAAGCCGCCG TCCCGTCAAG TCAGCGTAAT GCTCTGCCAG TGTTACAACC AATTAACCAA TTCTGATTAG AAAAACTCAT CGAGCATCAA ATGAAACTGC AATTTATTCA TATCAGGATT ATCAATACCA TATTTTTGAA AAAGCCGTTT CTGTAATGAA GGAGAAAACT CACCGAGGCA GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT GACGACTGAA TCCGGTGAGA ATGGCAGAAT AGGAACTTCG GAATAGGAAC TTCAAAGCGT TTCCGAAAAC GAGCGCTTCC GAAAATGCAA CGCGAGCTGC GCACATACAG CTCACTGTTC ACGTCGCACC TATATCTGCG TGTTGCCTGT ATATATATAT ACATGAGAAG AACGGCATAG TGCGTGTTTA TGCTTAAATG CGTACTTATA TGCGTCTATT TATGTAGGAT GAAAGGTAGT CTAGTACCTC CTGTGATATT ATCCCATTCC ATGCGGGGTA TCGTATGCTT CCTTCAGCAC TACCCTTTAG CTGTTCTATA TGCTGCCACT CCTCAATTGG ATTAGTCTCA TCCTTCAATG CTATCATTTC CTTTGATATT GGATCATATG CATAGTACCG AGAAACTAGT GCGAAGTAGT GATCAGGTAT TGCTGTTATC TGATGAGTAT ACGTTGTCCT GGCCACGGCA GAAGCACGCT TATCGCTCCA ATTTCCCACA ACATTAGTCA ACTCCGTTAG GCCCTTCATT GAAAGAAATG AGGTCATCAA ATGTCTTCCA ATGTGAGATT TTGGGCCATT TTTTATAGCA AAGATTGAAT AAGGCGCATT TTTCTTCAAA GCTTTATTGT ACGATCTGAC TAAGTTATCT TTTAATAATT GGTATTCCTG TTTATTGCTT GAAGAATTGC CGGTCCTATT TACTCGTTTT AGGACTGGTT CAGAATTCCT CAAAAATTCA TCCAAATATA CAAGTGGATC GATCCTACCC CTTGCGCTAA AGAAGTATAT GTGCCTACTA ACGCTTGTCT TTGTCTCTGT CACTAAACAC TGGATTATTA CTCCCAGATA CTTATTTTGG ACTAATTTAA ATGATTTCGG ATCAACGTTC TTAATATCGC TGAATCTTCC ACAATTGATG AAAGTAGCTA GGAAGAGGAA TTGGTATAAA GTTTTTGTTT TTGTAAATCT CGAAGTATAC TCAAACGAAT TTAGTATTTT CTCAGTGATC TCCCAGATGC TTTCACCCTC ACTTAGAAGT GCTTTAAGCA TTTTTTTACT GTGGCTATTT CCCTTATCTG CTTCTTCCGA TGATTCGAAC TGTAATTGCA AACTACTTAC AATATCAGTG ATATCAGATT GATGTTTTTG TCCATAGTAA GGAATAATTG TAAATTCCCA AGCAGGAATC AATTTCTTTA ATGAGGCTTC CAGAATTGTT GCTTTTTGCG TCTTGTATTT AAACTGGAGT GATTTATTGA CAATATCGAA ACTCAGCGAA TTGCTTATGA TAGTATTATA GCTCATGAAT GTGGCTCTCT TGATTGCTGT TCCGTTATGT GTAATCATCC AACATAAATA GGTTAGTTCA GCAGCACATA ATGCTATTTT CTCACCTGAA GGTCTTTCAA ACCTTTCCAC AAACTGACGA ACAAGCACCT TAGGTGGTGT TTTACATAAT ATATCAAATT GTGGCATACA ACCTCCTTAG TACATGCAAC CATTATCACC GCCAGAGGTA AAATAGTCAA CACGCACGGT GTTAGATATT TATCCCTTGC GGTGATAGAT TTAACGTATG AGCACAAAAA AGAAACCATT AACACAAGAG CAGCTTGAGG ACGCACGTCG CCTTAAAGCA ATTTATGAAA AAAAGAAAAA TGAACTTGGC TTATCCCAGG AATCTGTCGC AGACAAGATG GGGATGGGGC AGTCAGGCGT TGGTGCTTTA TTTAATGGCA TCAATGCATT AAATGCTTAT AACGCCGCAT TGCTTACAAA AATTCTCAAA GTTAGCGTTG AAGAATTTAG CCCTTCAATC GCCAGAGAAA TCTACGAGAT GTATGAAGCG GTTAGTATGC AGCCGTCACT TAGAAGTGAG TATGAGTACC CTGTTTTTTC TCATGTTCAG GCAGGGATGT TCTCACCTAA GCTTAGAACC TTTACCAAAG GTGATGCGGA GAGATGGGTA AGCACAACCA AAAAAGCCAG TGATTCTGCA TTCTGGCTTG AGGTTGAAGG TAATTCCATG ACCGCACCAA CAGGCTCCAA GCCAAGCTTT CCTGACGGAA TGTTAATTCT CGTTGACCCT GAGCAGGCTG TTGAGCCAGG TGATTTCTGC ATAGCCAGAC TTGGGGGTGA TGAGTTTACC TTCAAGAAAC TGATCAGGGA TAGCGGTCAG GTGTTTTTAC AACCACTAAA CCCACAGTAC CCAATGATCC CATGCAATGA GAGTTGTTCC GTTGTGGGGA AAGTTATCGC TAGTCAGTGG CCTGAAGAGA CGTTTGGCTG ATCGGCAAGG TGTTCTGGTC GGCGCATAGC TGATAACAAT TGAGCAAGAA TCTGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT TCCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA GATTGTCGCA CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC ATCCATGTTG GAATTTAATC GCGGCCTCGA GCAAGACGTT TCCCGTTGAA TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT TT
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