pWM91
$128.00
In stock
SKU
PL77603
100μg liquid plasmid(Instantly):$163
| name | pWM91 | another name | apWM91 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | R6K | |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCCCATG TCAGCCGTTA AGTGTTCCTG TGTCACTCAA AATTGCTTTG AGAGGCTCTA AGGGCTTCTC AGTGCGTTAC ATCCCTGGCT TGTTGTCCAC AACCGTTAAA CCTTAAAAGC TTTAAAAGCC TTATATATTC TTTTTTTTCT TATAAAACTT AAAACCTTAG AGGCTATTTA AGTTGCTGAT TTATATTAAT TTTATTGTTC AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTTAGCCAT GAGAGCTTAG TACGTTAGCC ATGAGGGTTT AGTTCGTTAA ACATGAGAGC TTAGTACGTT AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTACTATCA ACAGGTTGAA CTGCTGATCT TCAGATCCTC TACGCCGGAC GCATCGTGGG GATCTGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAGCGCG CGTAATACGA CTCACTATAG GGCGAATTGG GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCCTGCAGC CCGGGGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC AGATCCAGCC GACCAGGCTT TCCACGCCCG CGTGCCGCTC CATGTCGTTC GCGCGGTTCT CGGAAACGCG CTGCCGCGTT TCGTGATTGT CACGCTCAAG CCCGTAGTCC CGTTCGAGCG TCGCGCAGAG GTCAGCGAGG GCGCGGTAGG CCCGATACGG CTCATGGATG GTGTTTCGGG TCGGGTGAAT CTTGTTGATG GCGATATGGA TGTGCAGGTT GTCGGTGTCG TGATGCACGG CACTGACGCG CTGATGCTCG GCGAAGCCAA GCCCAGCGCA GATGCGGTCC TCAATCGCGC GCAACGTCTC CGCGTCGGGC TTCTCTCCCG CGCGGAAGCT AACCAGCAGG TGATAGGTCT TGTCGGCCTC GGAACGGGTG TTGCCGTGCT GGGTCGCCAT CACCTCGGCC ATGACAGCGG GCAGGGTGTT TGCCTCGCAG TTCGTGACGC GCACGTGACC CAGGCGCTCG GTCTTGCCTT GCTCGTCGGT GATGTACTTC ACCAGCTCCG CGAAGTCGCT CTTCTTGATG GAGCGCATGG GGACGTGCTT GGCAATCACG CGCACCCCCC GGCCGTTTTA GCGGCTAAAA AAGTCATGGC TCTGCCCTCG GGCGGACCAC GCCCATCATG ACCTTGCCAA GCTCGTCCTG CTTCTCTTCG ATCTTCGCCA GCAGGGCGAG GATCGTGGCA TCACCGAACC GCGCCGTGCG CGGGTCGTCG GTGAGCCAGA GTTTCAGCAG GCCGCCCAGG CGGCCCAGGT CGCCATTGAT GCGGGCCAGC TCGCGGACGT GCTCATAGTC CACGACGCCC GTGATTTTGT AGCCCTGGCC GACGGCCAGC AGGTAGGCCG ACAGGCTCAT GCCGGCCGCC GCCGCCTTTT CCTCAATCGC TCTTCGTTCG TCTGGAAGGC AGTACACCTT GATAGGTGGG CTGCCCTTCC TGGTTGGCTT GGTTTCATCA GCCATCCGCT TGCCCTCATC TGTTACGCCG GCGGTAGCCG GCCAGCCTCG CAGAGCAGGA TTCCCGTTGA GCACCGCCAG GTGCGAATAA GGGACAGTGA AGAAGGAACA CCCGCTCGCG GGTGGGCCTA CTTCACCTAT CCTGCCCGGC TGACGCCGTT GGATACACCA AGGAAAGTCT ACACGAACCC TTTGGCAAAA TCCTGTATAT CGTGCGAAAA AGGATGGATA TACCGAAAAA ATCGCTATAA TGACCCCGAA GCAGGGTTAT GCAGCGGAAA AGCGCTGCTT CCCTGCTGTT TTGTGGAATA TCTACCGACT GGAAACAGGC AAATGCAGGA AATTACTGAA CTGAGGGGAC AGGCGAGAGA CGATGCCAAA GAGCTACACC GACGAGCTGG CCGAGTGGGT TGAATCCCGC GCGGCCAAGA AGCGCCGGCG TGATGAGGCT GCGGTTGCGT TCCTGGCGGT GAGGGCGGAT GTCGAGGCGG CGTTAGCGTC CGGCTATGCG CTCGTCACCA TTTGGGAGCA CATGCGGGAA ACGGGGAAGG TCAAGTTCTC CTACGAGACG TTCCGCTCGC ACGCCAGGCG GCACATCAAG GCCAAGCCCG CCGATGTGCC CGCACCGCAG GCCAAGGCTG CGGAACCCGC GCCGGCACCC AAGACGCCGG AGCCACGGCG GCCGAAGCAG GGGGGCAAGG CTGAAAAGCC GGCCCCCGCT GCGGCCCCGA CCGGCTTCAC CTTCAACCCA ACACCGGACA AAAAGGATCT ATGATTCCCT TTGTCAACAG CAATGGATCA CTGAAAATGG TTCAATGATC ACATTAAGTG GTATTCAATA TTTTCATGAA ATGGGAATTG ACGTTCCTTC CAAACATTCA CGTAAAATCT GTTGTGCGTG TTTAGATTGG AGTGAACGCC GTTTCCATTT AGGTGGGTAC GTTGGAGCCG CATTATTTTC GCTTTATGAA TCTAAAGGGT GGTTAACTCG ACATCTTGGT TACCGTGAAG TTACCATCAC GGAAAAAGGT TATGCTGCTT TTAAGACCCA CTTTCACATT TAAGTTGTTT TTCTAATCCG CATATGATCA ATTCAAGGCC GAATAAGAAG GCTGGCTCTG CACCTTGGTG ATCAAATAAT TCGATAGCTT GTCGTAATAA TGGCGGCATA CTATCAGTAG TAGGTGTTTC CCTTTCTTCT TTAGCGACTT GATGCTCTTG ATCTTCCAAT ACGCAACCTA AAGTAAAATG CCCCACAGCG CTGAGTGCAT ATAATGCAGG TCGACTCTAG AGGATCGATC CTTTTTAACC CATCACATAT ACCTGCCGTT CACTATTATT TAGTGAAATG AGATATTATG ATATTTTCTG AATTGTGATT AAAAAGGCAA CTTTATGCCC ATGCAACAGA AACTATAAAA AATACAGAGA ATGAAAAGAA ACAGATAGAT TTTTTAGTTC TTTAGGCCCG TAGTCTGCAA ATCCTTTTAT GATTTTCTAT CAAACAAAAG AGGAAAATAG ACCAGTTGCA ATCCAAACGA GAGTCTAATA GAATGAGGTC GAAAAGTAAA TCGCGCGGGT TTGTTACTGA TAAAGCAGGC AAGACCTAAA ATGTGTAAAG GGCAAAGTGT ATACTTTGGC GTCACCCCTT ACATATTTTA GGTCTTTTTT TATTGTGCGT AACTAACTTG CCATCTTCAA ACAGGAGGGC TGGAAGAAGC AGACCGCTAA CACAGTACAT AAAAAAGGAG ACATGAACGA TGAACATCAA AAAGTTTGCA AAACAAGCAA CAGTATTAAC CTTTACTACC GCACTGCTGG CAGGAGGCGC AACTCAAGCG TTTGCGAAAG AAACGAACCA AAAGCCATAT AAGGAAACAT ACGGCATTTC CCATATTACA CGCCATGATA TGCTGCAAAT CCCTGAACAG CAAAAAAATG AAAAATATCA AGTTCCTGAA TTCGATTCGT CCACAATTAA AAATATCTCT TCTGCAAAAG GCCTGGACGT TTGGGACAGC TGGCCATTAC AAAACGCTGA CGGCACTGTC GCAAACTATC ACGGCTACCA CATCGTCTTT GCATTAGCCG GAGATCCTAA AAATGCGGAT GACACATCGA TTTACATGTT CTATCAAAAA GTCGGCGAAA CTTCTATTGA CAGCTGGAAA AACGCTGGCC GCGTCTTTAA AGACAGCGAC AAATTCGATG CAAATGATTC TATCCTAAAA GACCAAACAC AAGAATGGTC AGGTTCAGCC ACATTTACAT CTGACGGAAA AATCCGTTTA TTCTACACTG ATTTCTCCGG TAAACATTAC GGCAAACAAA CACTGACAAC TGCACAAGTT AACGTATCAG CATCAGACAG CTCTTTGAAC ATCAACGGTG TAGAGGATTA TAAATCAATC TTTGACGGTG ACGGAAAAAC GTATCAAAAT GTACAGCAGT TCATCGATGA AGGCAACTAC AGCTCAGGCG ACAACCATAC GCTGAGAGAT CCTCACTACG TAGAAGATAA AGGCCACAAA TACTTAGTAT TTGAAGCAAA CACTGGAACT GAAGATGGCT ACCAAGGCGA AGAATCTTTA TTTAACAAAG CATACTATGG CAAAAGCACA TCATTCTTCC GTCAAGAAAG TCAAAAACTT CTGCAAAGCG ATAAAAAACG CACGGCTGAG TTAGCAAACG GCGCTCTCGG TATGATTGAG CTAAACGATG ATTACACACT GAAAAAAGTG ATGAAACCGC TGATTGCATC TAACACAGTA ACAGATGAAA TTGAACGCGC GAACGTCTTT AAAATGAACG GCAAATGGTA CCTGTTCACT GACTCCCGCG GATCAAAAAT GACGATTGAC GGCATTACGT CTAACGATAT TTACATGCTT GGTTATGTTT CTAATTCTTT AACTGGCCCA TACAAGCCGC TGAACAAAAC TGGCCTTGTG TTAAAAATGG ATCTTGATCC TAACGATGTA ACCTTTACTT ACTCACACTT CGCTGTACCT CAAGCGAAAG GAAACAATGT CGTGATTACA AGCTATATGA CAAACAGAGG ATTCTACGCA GACAAACAAT CAACGTTTGC GCCAAGCTTC CTGCTGAACA TCAAAGGCAA GAAAACATCT GTTGTCAAAG ACAGCATCCT TGAACAAGGA CAATTAACAG TTAACAAATA AAAACGCAAA AGAAAATGCC GATATCCTAT TGGCATTTTC TTTTATTTCT TATCAACATA AAGGTGAATC CCATATGAAC TATATAAAAG CAGGCAAATG GCTAACCGTA TTCCTAACCT TTTGGTAATG ACTCCAACTT ATTGATAGTG TTTTATGTTC AGATAATGCC CGATGACTTT GTCATGCAGC TCCACCGATT TTGAGAACGA CAGCGACTTC CGTCCCAGCC GTGCCAGGTG CTGCCTCAGA TTCAGGTTAT GCCGCTCAAT TCGCTGCGTA TATCGCTTGC TGATTACGTG CAGCTTTCCC TTCAGGCGGG ATTCATACAG CGGCCAGCCA TCCGTCATCC ATATCACCAC GTCAAAGGGT GACAGCAGGC TCATAAGACG CCCCAGCGTC GCCATAGTGC GTTCACCGAA TACGTGCGCA ACAACCGTCT TCCGGAGACT GTCATACGCG TAAAACAGCC AGCGCTGGCG CGATTTAGCC CCGACATAGC CCCACTGTTC GTCCATTTCC GCGCAGACGA TGACGTCACT GCCCGGCTGT ATGCGCGAGG TTACCGACTG CGGCCTGAGT TTTTTAAGTG ACGTAAAATC GTGTTGAGGC CAACGCCCAT AATGCGGGCT GTTGCCCGGC ATCCAACGCC ATTCATGGCC ATATCAATGA TTTTCTGGTG CGTACCGGGT TGAGAAGCGG TGTAAGTGAA CTGCATAAAC CAGCCATTGA GTAAGTTTTT AAGCACATCA CTATCATAAG CTTTAAGTTG GTTCTCTTGG ATCAATTTGC TGACAATGGC GTTTACCTTA CCAGTAATGT ATTCAAGGCT AATTTTTTCA AGTTCATTCC AACCAATGAT AGGCATCACT TCTTGGATAG GGATAAGGTT TTTATTATTA TCAATAATAT AATCAAGATA ATGTTCAAAT ATACTTTCTA AGGCAGACCA ACCATTTGTT AAATCAGTTT TTGTTGTGAT GTAGGCATCA ATCATAATTA ATTGCTGCTT ATAACAGGCA CTGAGTAATT GTTTTTTATT TTTAAAGTGA TGATAAAAGG CACCTTTGGT CACCAACGCT TTTCCCGAGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTGGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAG
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