pJQ200SK
$128.00
In stock
SKU
PL77601
100μg liquid plasmid(Instantly):$163
| name | pJQ200SK | another name | apJQ200SK |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Gen |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | p15A | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTGCCATCT TCAAACAGGA GGGCTGGAAG AAGCAGACCG CTAACACAGT ACATAAAAAA GGAGACATGA ACGATGAACA TCAAAAAGTT TGCAAAACAA GCAACAGTAT TAACCTTTAC TACCGCACTG CTGGCAGGAG GCGCAACTCA AGCGTTTGCG AAAGAAACGA ACCAAAAGCC ATATAAGGAA ACATACGGCA TTTCCCATAT TACACGCCAT GATATGCTGC AAATCCCTGA ACAGCAAAAA AATGAAAAAT ATCAAGTTCC TGAATTCGAT TCGTCCACAA TTAAAAATAT CTCTTCTGCA AAAGGCCTGG ACGTTTGGGA CAGCTGGCCA TTACAAAACG CTGACGGCAC TGTCGCAAAC TATCACGGCT ACCACATCGT CTTTGCATTA GCCGGAGATC CTAAAAATGC GGATGACACA TCGATTTACA TGTTCTATCA AAAAGTCGGC GAAACTTCTA TTGACAGCTG GAAAAACGCT GGCCGCGTCT TTAAAGACAG CGACAAATTC GATGCAAATG ATTCTATCCT AAAAGACCAA ACACAAGAAT GGTCAGGTTC AGCCACATTT ACATCTGACG GAAAAATCCG TTTATTCTAC ACTGATTTCT CCGGTAAACA TTACGGCAAA CAAACACTGA CAACTGCACA AGTTAACGTA TCAGCATCAG ACAGCTCTTT GAACATCAAC GGTGTAGAGG ATTATAAATC AATCTTTGAC GGTGACGGAA AAACGTATCA AAATGTACAG CAGTTCATCG ATGAAGGCAA CTACAGCTCA GGCGACAACC ATACGCTGAG AGATCCTCAC TACGTAGAAG ATAAAGGCCA CAAATACTTA GTATTTGAAG CAAACACTGG AACTGAAGAT GGCTACCAAG GCGAAGAATC TTTATTTAAC AAAGCATACT ATGGCAAAAG CACATCATTC TTCCGTCAAG AAAGTCAAAA ACTTCTGCAA AGCGATAAAA AACGCACGGC TGAGTTAGCA AACGGCGCTC TCGGTATGAT TGAGCTAAAC GATGATTACA CACTGAAAAA AGTGATGAAA CCGCTGATTG CATCTAACAC AGTAACAGAT GAAATTGAAC GCGCGAACGT CTTTAAAATG AACGGCAAAT GGTACCTGTT CACTGACTCC CGCGGATCAA AAATGACGAT TGACGGCATT ACGTCTAACG ATATTTACAT GCTTGGTTAT GTTTCTAATT CTTTAACTGG CCCATACAAG CCGCTGAACA AAACTGGCCT TGTGTTAAAA ATGGATCTTG ATCCTAACGA TGTAACCTTT ACTTACTCAC ACTTCGCTGT ACCTCAAGCG AAAGGAAACA ATGTCGTGAT TACAAGCTAT ATGACAAACA GAGGATTCTA CGCAGACAAA CAATCAACGT TTGCGCCAAG CTTCCTGCTG AACATCAAAG GCAAGAAAAC ATCTGTTGTC AAAGACAGCA TCCTTGAACA AGGACAATTA ACAGTTAACA AATAAAAACG CAAAAGAAAA TGCCGATGGC CGCGGCGTTG TGACAATTTA CCGAACAACT CCGCGGCCGG GAAGCCGATC TCGGCTTGAA CGAATTGTTA GGTGGCGGTA CTTGGGTCGA TATCAAAGTG CATCACTTCT TCCCGTATGC CCAACTTTGT ATAGAGAGCC ACTGCGGGAT CGTCACCGTA ATCTGCTTGC ACGTAGATCA CATAAGCACC AAGCGCGTTG GCCTCATGCT TGAGGAGATT GATGAGCGCG GTGGCAATGC CCTGCCTCCG GTGCTCGCCG GAGACTGCGA GATCATAGAT ATAGATCTCA CTACGCGGCT GCTCAAACTT GGGCAGAACG TAAGCCGCGA GAGCGCCAAC AACCGCTTCT TGGTCGAAGG CAGCAAGCGC GATGAATGTC TTACTACGGA GCAAGTTCCC GAGGTAATCG GAGTCCGGCT GATGTTGGGA GTAGGTGGCT ACGTCTCCGA ACTCACGACC GAAAAGATCA AGAGCAGCCC GCATGGATTT GACTTGGTCA GGGCCGAGCC TACATGTGCG AATGATGCCC ATACTTGAGC CACCTAACTT TGTTTTAGGG CGACTGCCCT GCTGCGTAAC ATCGTTGCTG CTGCGTAACA TCGTTGCTGC TCCATAACAT CAAACATCGA CCCACGGCGT AACGCGCTTG CTGCTTGGAT GCCCGAGGCA TAGACTGTAC AAAAAAACAG TCATAACAAG CCATGAAAAC CGCCACTGCG CCGTTACCAC CGCTGCGTTC GGTCAAGGTT CTGGACCAGT TGCGTGAGCG CATACGCTAC TTGCATTACA GTTTACGAAC CGAACAGGCT TATGTCAACT GGGTTCGTGC CTTCATCCGT TTCCACGGTG TGCGTCACCC GGCAACCTTG GGCAGCAGCG AAGTCGAGGC ATTTCTGTCC TGGCTGGCGA ACGAGCGCAA GGTTTCGGTC TCCACGCATC GTCAGGCATT GGCGGCCTTG CTGTTCTTCT ACGGCAAGGT GCTGTGCACG GATCTGCCCT GGCTTCAGGA GATCGGAAGA CCTCGGCCGT CGCGGCGCTT GCCGGTGGTG CTGACCCCGG ATGAAGTGGT TCGCATCCTC GGTTTTCTGG AAGGCGAGCA TCGTTTGTTC GCCCAGCTTC TGTATGGAAC GGGCATGCGG ATCAGTGAGG GTTTGCAACT GCGGGTCAAG GATCTGGATT TCGATCACGG CACGATCATC GTGCGGGAGG GCAAGGGCTC CAAGGATCGG GCCTTGATGT TACCCGAGAG CTTGGCACCC AGCCTGCGCG AGCAGAATTA ATTCCCCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAGCGCG CGTAATACGA CTCACTATAG GGCGAATTGG GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCCTGCAGC CCGGGGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGGGGAAT TAATTCTGTC CCTCCTGTTC AGCTACTGAC GGGGTGGTGC GTAACGGCAA AAGCACCGCC GGACATCAGC GCTAGCGGAG TGTATACTGG CTTACTATGT TGGCACTGAT GAGGGTGTCA GTGAAGTGCT TCATGTGGCA GGAGAAAAAA GGCTGCACCG GTGCGTCAGC AGAATATGTG ATACAGGATA TATTCCGCTT CCTCGCTCAC TGACTCGCTA CGCTCGGTCG TTCGACTGCG GCGAGCGGAA ATGGCTTACG AACGGGGCGG AGATTTCCTG GAAGATGCCA GGAAGATACT TAACAGGGAA GTGAGAGGGC CGCGGCAAAG CCGTTTTTCC ATAGGCTCCG CCCCCCTGAC AAGCATCACG AAATCTGACG CTCAAATCAG TGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG CGGCTCCCTC GTGCGCTCTC CTGTTCCTGC CTTTCGGTTT ACCGGTGTCA TTCCGCTGTT ATGGCCGCGT TTGTCTCATT CCACGCCTGA CACTCAGTTC CGGGTAGGCA GTTCGCTCCA AGCTGGACTG TATGCACGAA CCCCCCGTTC AGTCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GAAAGACATG CAAAAGCACC ACTGGCAGCA GCCACTGGTA ATTGATTTAG AGGAGTTAGT CTTGAAGTCA TGCGCCGGTT AAGGCTAAAC TGAAAGGACA AGTTTTGGTG ACTGCGCTCC TCCAAGCCAG TTACCTCGGT TCAAAGAGTT GGTAGCTCAG AGAACCTTCG AAAAACCGCC CTGCAAGGCG GTTTTTTCGT TTTCAGAGCA AGAGATTACG CGCAGACCAA AACGATCTCA AGAAGATCAT CTTATTAATC AGATAAAATA TTTCTAGCTA GAGTCGATCT TCGCCAGCAG GGCGAGGATC GTGGCATCAC CGAACCGCGC CGTGCGCGGG TCGTCGGTGA GCCAGAGTTT CAGCAGGCCG CCCAGGCGGC CCAGGTCGCC ATTGATGCGG GCCAGCTCGC GGACGTGCTC ATAGTCCACG ACGCCCGTGA TTTTGTAGCC CTGGCCGACG GCCAGCAGGT AGGCCGACAG GCTCATGCCG GCCGCCGCCG CCTTTTCCTC AATCGCTCTT CGTTCGTCTG GAAGGCAGTA CACCTTGATA GGTGGGCTGC CCTTCCTGGT TGGCTTGGTT TCATCAGCCA TCCGCTTGCC CTCATCTGTT ACGCCGGCGG TAGCCGGCCA GCCTCGCAGA GCAGGATTCC CGTTGAGCAC CGCCAGGTGC GAATAAGGGA CAGTGAAGAA GGAACACCCG CTCGCGGGTG GGCCTACTTC ACCTATCCTG CCCGGCTGAC GCCGTTGGAT ACACCAAGGA AAGTCTACAC GAACCCTTTG GCAAAATCCT GTATATCGTG CGAAAAAGGA TGGATATACC GAAAAAATCG CTATAATGAC CCCGAAGCAG GGTTATGCAG CGGAAAAGCG CTGCTTCCCT GCTGTTTTGT GGAATATCTA CCGACTGGAA ACAGGCAAAT GCAGGAAATT ACTGAACTGA GGGGACAGGC GAGAGACGAT GCCAAAGAGC TACACCGACG AGCTGGCCGA GTGGGTTGAA TCCCGCGCGG CCAAGAAGCG CCGGCGTGAT GAGGCTGCGG TTGCGTTCCT GGCGGTGAGG GCGGATGTCG ATCGACTCTA GCTAGAGGAT CGATCCTTTT TAACCCATCA CATATACCTG CCGTTCACTA TTATTTAGTG AAATGAGATA TTATGATATT TTCTGAATTG TGATTAAAAA GGCAACTTTA TGCCCATGCA ACAGAAACTA TAAAAAATAC AGAGAATGAA AAGAAACAGA TAGATTTTTT AGTTCTTTAG GCCCGTAGTC TGCAAATCCT TTTATGATTT TCTATCAAAC AAAAGAGGAA AATAGACCAG TTGCAATCCA AACGAGAGTC TAATAGAATG AGGTCGAAAA GTAAATCGCG CGGGTTTGTT ACTGATAAAG CAGGCAAGAC CTAAAATGTG TAAAGGGCAA AGTGTATACT TTGGCGTCAC CCCTTACATA TTTTAGGTCT TTTTTTATTG TGCGTAACTA A
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