pCDNA3-PTK7-VSV
$99.00
In stock
SKU
PL77600
NM_002821.5;CCK-4;CCK4
100μg liquid plasmid(Instantly):$120
| name | pCDNA3-PTK7-VSV | another name | apCDNA3-PTK7-VSV |
| Insert Size (bp) | 3213bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACTAGTAA CGGCCGCCAG TGTGCTGGAA TTCGCGCCCT CAGCTCCTTT TCCTGAGCCC GCCGCGATGG GAGCTGCGCG GGGATCCCCG GCCAGACCCC GCCGGTTGCC TCTGCTCAGC GTCCTGCTGC TGCCGCTGCT GGGCGGTACC CAGACAGCCA TTGTCTTCAT CAAGCAGCCG TCCTCCCAGG ATGCACTGCA GGGGCGCCGG GCGCTGCTTC GCTGTGAGGT TGAGGCTCCG GGCCCGGTAC ATGTGTACTG GCTGCTCGAT GGGGCCCCTG TCCAGGACAC GGAGCGGCGT TTCGCCCAGG GCAGCAGCCT GAGCTTTGCA GCTGTGGACC GGCTGCAGGA CTCTGGCACC TTCCAGTGTG TGGCTCGGGA TGATGTCACT GGAGAAGAAG CCCGCAGTGC CAACGCCTCC TTCAACATCA AATGGATTGA GGCAGGTCCT GTGGTCCTGA AGCATCCAGC CTCGGAAGCT GAGATCCAGC CACAGACCCA GGTCACACTT CGTTGCCACA TTGATGGGCA CCCTCGGCCC ACCTACCAAT GGTTCCGAGA TGGGACCCCC CTTTCTGATG GTCAGAGCAA CCACACAGTC AGCAGCAAGG AGCGGAACCT GACGCTCCGG CCAGCTGGTC CTGAGCATAG TGGGCTGTAT TCCTGCTGCG CCCACAGTGC TTTTGGCCAG GCTTGCAGCA GCCAGAACTT CACCTTGAGC ATTGCTGATG AAAGCTTTGC CAGGGTGGTG CTGGCACCCC AGGACGTGGT AGTAGCGAGG TATGAGGAGG CCATGTTCCA TTGCCAGTTC TCAGCCCAGC CACCCCCGAG CCTGCAGTGG CTCTTTGAGG ATGAGACTCC CATCACTAAC CGCAGTCGCC CCCCACACCT CCGCAGAGCC ACAGTGTTTG CCAACGGGTC TCTGCTGCTG ACCCAGGTCC GGCCACGCAA TGCAGGGATC TACCGCTGCA TTGGCCAGGG GCAGAGGGGC CCACCCATCA TCCTGGAAGC CACACTTCAC CTAGCAGAGA TTGAAGACAT GCCGCTATTT GAGCCACGGG TGTTTACAGC TGGCAGCGAG GAGCGTGTGA CCTGCCTTCC CCCCAAGGGT CTGCCAGAGC CCAGCGTGTG GTGGGAGCAC GCGGGAGTCC GGCTGCCCAC CCATGGCAGG GTCTACCAGA AGGGCCACGA GCTGGTGTTG GCCAATATTG CTGAAAGTGA TGCTGGTGTC TACACCTGCC ACGCGGCCAA CCTGGCTGGT CAGCGGAGAC AGGATGTCAA CATCACTGTG GCCACTGTGC CCTCCTGGCT GAAGAAGCCC CAAGACAGCC AGCTGGAGGA GGGCAAACCC GGCTACTTGG ATTGCCTGAC CCAGGCCACA CCAAAACCTA CAGTTGTCTG GTACAGAAAC CAGATGCTCA TCTCAGAGGA CTCACGGTTC GAGGTCTTCA AGAATGGGAC CTTGCGCATC AACAGCGTGG AGGTGTATGA TGGGACATGG TACCGTTGTA TGAGCAGCAC CCCAGCCGGC AGCATCGAGG CGCAAGCCCG TGTCCAAGTG CTGGAAAAGC TCAAGTTCAC ACCACCACCC CAGCCACAGC AGTGCATGGA GTTTGACAAG GAGGCCACGG TGCCCTGTTC AGCCACAGGC CGAGAGAAGC CCACTATTAA GTGGGAACGG GCAGATGGGA GCAGCCTCCC AGAGTGGGTG ACAGACAACG CTGGGACCCT GCATTTTGCC CGGGTGACTC GAGATGACGC TGGCAACTAC ACTTGCATTG CCTCCAACGG GCCGCAGGGC CAGATTCGTG CCCATGTCCA GCTCACTGTG GCAGTTTTTA TCACCTTCAA AGTGGAACCA GAGCGTACGA CTGTGTACCA GGGCCACACA GCCCTACTGC AGTGCGAGGC CCAGGGGGAC CCCAAGCCGC TGATTCAGTG GAAAGGCAAG GACCGCATCC TGGACCCCAC CAAGCTGGGA CCCAGGATGC ACATCTTCCA GAATGGCTCC CTGGTGATCC ATGACGTGGC CCCTGAGGAC TCAGGCCGCT ACACCTGCAT TGCAGGCAAC AGCTGCAACA TCAAGCACAC GGAGGCCCCC CTCTATGTCG TGGACAAGCC TGTGCCGGAG GAGTCGGAGG GCCCTGGCAG CCCTCCCCCC TACAAGATGA TCCAGACCAT TGGGTTGTCG GTGGGTGCCG CTGTGGCCTA CATCATTGCC GTGCTGGGCC TCATGTTCTA CTGCAAGAAG CGCTGCAAAG CCAAGCGGCT GCAGAAGCAG CCCGAGGGCG AGGAGCCAGA GATGGAATGC CTCAACGGTG GGCCTTTGCA GAACGGGCAG CCCTCAGCAG AGATCCAAGA AGAAGTGGCC TTGACCAGCT TGGGCTCCGG CCCCGCGGCC ACCAACAAAC GCCACAGCAC AAGTGATAAG ATGCACTTCC CACGGTCTAG CCTGCAGCCC ATCACCACGC TGGGGAAGAG TGAGTTTGGG GAGGTGTTCC TGGCAAAGGC TCAGGGCTTG GAGGAGGGAG TGGCAGAGAC CCTGGTACTT GTGAAGAGCC TGCAGAGCAA GGATGAGCAG CAGCAGCTGG ACTTCCGGAG GGAGTTGGAG ATGTTTGGGA AGCTGAACCA CGCCAACGTG GTGCGGCTCC TGGGGCTGTG CCGGGAGGCT GAGCCCCACT ACATGGTGCT GGAATATGTG GATCTGGGAG ACCTCAAGCA GTTCCTGAGG ATTTCCAAGA GCAAGGATGA AAAATTGAAG TCACAGCCCC TCAGCACCAA GCAGAAGGTG GCCCTATGCA CCCAGGTAGC CCTGGGCATG GAGCACCTGT CCAACAACCG CTTTGTGCAT AAGGACTTGG CTGCGCGTAA CTGCCTGGTC AGTGCCCAGA GACAAGTGAA GGTGTCTGCC CTGGGCCTCA GCAAGGATGT GTACAACAGT GAGTACTACC ACTTCCGCCA GGCCTGGGTG CCGCTGCGCT GGATGTCCCC CGAGGCCATC CTGGAGGGTG ACTTCTCTAC CAAGTCTGAT GTCTGGGCCT TCGGTGTGCT GATGTGGGAA GTGTTTACAC ATGGAGAGAT GCCCCATGGT GGGCAGGCAG ATGATGAAGT ACTGGCAGAT TTGCAGGCTG GGAAGGCTAG ACTTCCTCAG CCCGAGGGCT GCCCTTCCAA ACTCTATCGG CTGATGCAGC GCTGCTGGGC CCTCAGCCCC AAGGACCGGC CCTCCTTCAG TGAGATTGCC AGCGCCCTGG GAGACAGCAC CGTGGACAGC AAGCCGTCTA GAGGGCCCTA CACCGACATC GAGATGAACC GGCTGGGCAA GTAACTCGAG CTAGCGGCCG CGGCTATAGT GTCACCTAAA TGCTAGAGCT CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C
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