psiCHECK2-ICAM-1-3'UTR
$84.00
In stock
SKU
PL77578
100μg liquid plasmid(Instantly):$105
| name | psiCHECK2-ICAM-1-3'UTR | another name | apsiCHECK2-ICAM-1-3'UTR |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | psiCHECK-2-F： | |
| 3′ sequencing primer | psiCHECK-2-R： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGAC CTATCCCGGG ACAGGGCCTC TTCCTCGGCC TTCCCATATT GGTGGCAGTG GTGCCACACT GAACAGAGTG GAAGACATAT GCCATGCAGC TACACCTACC GGCCCTGGGA CGCCGGAGGA CAGGGCATTG TCCTCAGTCA GATACAACAG CATTTGGGGC CATGGTACCT GCACACCTAA AACACTAGGC CACGCATCTG ATCTGTAGTC ACATGACTAA GCCAAGAGGA AGGAGCAAGA CTCAAGACAT GATTGATGGA TGTTAAAGTC TAGCCTGATG AGAGGGGAAG TGGTGGGGGA GACATAGCCC CACCATGAGG ACATACAACT GGGAAATACT GAAACTTGCT GCCTATTGGG TATGCTGAGG CCCCACAGAC TTACAGAAGA AGTGGCCCTC CATAGACATG TGTAGCATCA AAACACAAAG GCCCACACTT CCTGACGGAT GCCAGCTTGG GCACTGCTGT CTACTGACCC CAACCCTTGA TGATATGTAT TTATTCATTT GTTATTTTAC CAGCTATTTA TTGAGTGTCT TTTATGTAGG CTAAATGAAC ATAGGTCTCT GGCCTCACGG AGCTCCCAGT CCTAATCACA TTCAAGGTCA CCAGGTACAG TTGTACAGGT TGTACACTGC AGGAGAGTGC CTGGCAAAAA GATCAAATGG GGCTGGGACT TCTCATTGGC CAACCTGCCT TTCCCCAGAA GGAGTGATTT TTCTATCGGC ACAAAAGCAC TATATGGACT GGTAATGGTT ACAGGTTCAG AGATTACCCA GTGAGGCCTT ATTCCTCCCT TCCCCCCAAA ACTGACACCT TTGTTAGCCA CCTCCCCACC CACATACATT TCTGCCAGTG TTCACAATGA CACTCAGCGG TCATGTCTGG ACATGAGTGC CCAGGGAATA TGCCCAAGCT ATGCCTTGTC CTCTTGTCCT GTTTGCATTT CACTGGGAGC TTGCACTATG CAGCTCCAGT TTCCTGCAGT GATCAGGGTC CTGCAAGCAG TGGGGAAGGG GGCCAAGGTA TTGGAGGACT CCCTCCCAGC TTTGGAAGCC TCATCCGCGT GTGTGTGTGT GTGTATGTGT AGACAAGCTC TCGCTCTGTC ACCCAGGCTG GAGTGCAGTG GTGCAATCAT GGTTCACTGC AGTCTTGACC TTTTGGGCTC AAGTGATCCT CCCACCTCAG CCTCCTGAGT AGCTGGGACC ATAGGCTCAC AACACCACAC CTGGCAAATT TGATTTTTTT TTTTTTTCCA GAGACGGGGT CTCGCAACAT TGCCCAGACT TCCTTTGTGT TAGTTAATAA AGCTTTCTCA ACTGCCGCGG CCGCTGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG AGGATCTAAA TGAGTCTTCG GACCTCGCGG GGGCCGCTTA AGCGGTGGTT AGGGTTTGTC TGACGCGGGG GGAGGGGGAA GGAACGAAAC ACTCTCATTC GGAGGCGGCT CGGGGTTTGG TCTTGGTGGC CACGGGCACG CAGAAGAGCG CCGCGATCCT CTTAAGCACC CCCCCGCCCT CCGTGGAGGC GGGGGTTTGG TCGGCGGGTG GTAACTGGCG GGCCGCTGAC TCGGGCGGGT CGCGCGCCCC AGAGTGTGAC CTTTTCGGTC TGCTCGCAGA CCCCCGGGCG GCGCCGCCGC GGCGGCGACG GGCTCGCTGG GTCCTAGGCT CCATGGGGAC CGTATACGTG GACAGGCTCT GGAGCATCCG CACGACTGCG GTGATATTAC CGGAGACCTT CTGCGGGACG AGCCGGGTCA CGCGGCTGAC GCGGAGCGTC CGTTGGGCGA CAAACACCAG GACGGGGCAC AGGTACACTA TCTTGTCACC CGGAGGCGCG AGGGACTGCA GGAGCTTCAG GGAGTGGCGC AGCTGCTTCA TCCCCGTGGC CCGTTGCTCG CGTTTGCTGG CGGTGTCCCC GGAAGAAATA TATTTGCATG TCTTTAGTTC TATGATGACA CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA GTCGGGGCGG CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA CACCGAGCGA CCCTGCAGCG ACCCGCTTAA AAGCTTGGCA TTCCGGTACT GTTGGTAAAG CCACCATGGC CGATGCTAAG AACATTAAGA AGGGCCCTGC TCCCTTCTAC CCTCTGGAGG ATGGCACCGC TGGCGAGCAG CTGCACAAGG CCATGAAGAG GTATGCCCTG GTGCCTGGCA CCATTGCCTT CACCGATGCC CACATTGAGG TGGACATCAC CTATGCCGAG TACTTCGAGA TGTCTGTGCG CCTGGCCGAG GCCATGAAGA GGTACGGCCT GAACACCAAC CACCGCATCG TGGTGTGCTC TGAGAACTCT CTGCAGTTCT TCATGCCAGT GCTGGGCGCC CTGTTCATCG GAGTGGCCGT GGCCCCTGCT AACGACATTT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATTTCTCA GCCTACCGTG GTGTTCGTGT CTAAGAAGGG CCTGCAGAAG ATCCTGAACG TGCAGAAGAA GCTGCCTATC ATCCAGAAGA TCATCATCAT GGACTCTAAG ACCGACTACC AGGGCTTCCA GAGCATGTAC ACATTCGTGA CATCTCATCT GCCTCCTGGC TTCAACGAGT ACGACTTCGT GCCAGAGTCT TTCGACAGGG ACAAAACCAT TGCCCTGATC ATGAACAGCT CTGGGTCTAC CGGCCTGCCT AAGGGCGTGG CCCTGCCTCA TCGCACCGCC TGTGTGCGCT TCTCTCACGC CCGCGACCCT ATTTTCGGCA ACCAGATCAT CCCCGACACC GCTATTCTGA GCGTGGTGCC ATTCCACCAC GGCTTCGGCA TGTTCACCAC CCTGGGCTAC CTGATTTGCG GCTTTCGGGT GGTGCTGATG TACCGCTTCG AGGAGGAGCT GTTCCTGCGC AGCCTGCAAG ACTACAAAAT TCAGTCTGCC CTGCTGGTGC CAACCCTGTT CAGCTTCTTC GCTAAGAGCA CCCTGATCGA CAAGTACGAC CTGTCTAACC TGCACGAGAT TGCCTCTGGC GGCGCCCCAC TGTCTAAGGA GGTGGGCGAA GCCGTGGCCA AGCGCTTTCA TCTGCCAGGC ATCCGCCAGG GCTACGGCCT GACCGAGACA ACCAGCGCCA TTCTGATTAC CCCAGAGGGC GACGACAAGC CTGGCGCCGT GGGCAAGGTG GTGCCATTCT TCGAGGCCAA GGTGGTGGAC CTGGACACCG GCAAGACCCT GGGAGTGAAC CAGCGCGGCG AGCTGTGTGT GCGCGGCCCT ATGATTATGT CCGGCTACGT GAATAACCCT GAGGCCACAA ACGCCCTGAT CGACAAGGAC GGCTGGCTGC ACTCTGGCGA CATTGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGCCTGA AGTCTCTGAT CAAGTACAAG GGCTACCAGG TGGCCCCAGC CGAGCTGGAG TCTATCCTGC TGCAGCACCC TAACATTTTC GACGCCGGAG TGGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCTGC CGCCGTCGTC GTGCTGGAAC ACGGCAAGAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTGACAACCG CCAAGAAGCT GCGCGGCGGA GTGGTGTTCG TGGACGAGGT GCCCAAGGGC CTGACCGGCA AGCTGGACGC CCGCAAGATC CGCGAGATCC TGATCAAGGC TAAGAAAGGC GGCAAGATCG CCGTGTAATA ATTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG GCTCGAC
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