pAbAi-Kan
$128.00
In stock
SKU
PL77539
100μg liquid plasmid(Instantly):$149
| name | pAbAi-Kan | another name | apAbAi-Kan |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | URA3 | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pABAI-F：GTTCCTTATATGTAGCTTTCGACA | |
| 3′ sequencing primer | pABAI-R：CCATCTCGAAAAAGGGTTTGCC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGCTTGAAT TCGAGCTCGG TACCCGGGGA TCTGTCGACC TCGAGGCATG TGCTCTGTAT GTATATAAAA CTCTTGTTTT CTTCTTTTCT CTAAATATTC TTTCCTTATA CATTAGGTCC TTTGTAGCAT AAATTACTAT ACTTCTATAG ACACGCAAAC ACAAATACAC ACACTAAATT AATAATGGCA AACCCTTTTT CGAGATGGTT TCTATCAGAG AGACCTCCAA ACTGCCATGT AGCCGATTTA GAAACAAGTT TAGATCCCCA TCAAACGTTG TTGAAGGTGC AAAAATACAA ACCCGCTTTA AGCGACTGGG TGCATTACAT CTTCTTGGGA TCCATCATGC TGTTTGTGTT CATTACTAAT CCCGCACCTT GGATCTTCAA GATCCTTTTT TATTGTTTCT TGGGCACTTT ATTCATCATT CCAGCTACGT CACAGTTTTT CTTCAATGCC TTGCCCATCC TAACATGGGT GGCGCTGTAT TTCACTTCAT CGTACTTTCC AGATGACCGC AGGCCTCCTA TTACTGTCAA AGTGTTACCA GCGGTGGAAA CAATTTTATA CGGCGACAAT TTAAGTGATA TTCTTGCAAC ATCGACGAAT TCCTTTTTGG ACATTTTAGC ATGGTTACCG TACGGACTAT TTCATTATGG GGCCCCATTT GTCGTTGCTG CCATCTTATT CGTATTTGGT CCACCAACTG TTTTGCAAGG TTATGCTTTT GCATTTGGTT ATATGAACCT GTTTGGTGTT ATCATGCAAA ATGTCTTTCC AGCCGCTCCC CCATGGTATA AAATTCTCTA TGGATTGCAA TCAGCCAACT ATGATATGCA TGGCTCGCCT GGTGGATTAG CTAGAATTGA TAAGCTACTC GGTATTAATA TGTATACTAC ATGTTTTTCA AATTCCTCCG TCATTTTCGG TGCTTTTCCT TCACTGCATT CCGGGTGTGC TACTATGGAA GCCCTGTTTT TCTGTTATTG TTTTCCAAAA TTGAAGCCCT TGTTTATTGC TTATGTTTGC TGGTTATGGT GGTCAACTAT GTATCTGACA CACCATTATT TTGTAGACCT TATGGCAGGT TCTGTGCTGT CATACGTTAT TTTCCAGTAC ACAAAGTACA CACATTTACC AATTGTAGAT ACATCTCTTT TTTGCAGATG GTCATACACT TCAATTGAGA AATACGATAT ATCAAAGAGT GATCCATTGG CTGCAGATTC AAACGATATC GAAAGTGTCC CTTTGTCCAA CTTGGAACTT GACTTTGATC TTAATATGAC TGATGAACCC AGTGTAAGCC CTTCGTTATT TGATGGATCT ACTTCTGTTT CTCGTTCGTC CGCCACGTCT ATAACGTCAC TAGGTGTAAA GAGGGCTTAA GGAAATCCAT TATGTACTAT TTAAAAAACA CAAACTTTTG GATGTTCGGT TTATTCTTTT TCTTTTACTT TTTTATCATG GGAGCCTACT TCCCGTTTTT CCCGATTTGG CTACATGACA TCAACCATAT CAGCAAAAGT GATACGGGTA TTATTTTTGC CGCTATTTCT CTGTTCTCGC TATTATTCCA ACCGCTGTTT GGTCTGCTTT CTGACAAACT CGGCCTCGAC TCTAGCGAAT TAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGTCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GGGTAATAAC TGATATAATT AAATTGAAGC TCTAATTTGT GAGTTTAGTA TACATGCATT TACTTATAAT ACAGTTTTTT AGTTTTGCTG GCCGCATCTT CTCAAATATG CTTCCCAGCC TGCTTTTCTG TAACGTTCAC CCTCTACCTT AGCATCCCTT CCCTTTGCAA ATAGTCCTCT TCCAACAATA ATAATGTCAG ATCCTGTAGA GACCACATCA TCCACGGTTC TATACTGTTG ACCCAATGCG TCTCCCTTGT CATCTAAACC CACACCGGGT GTCATAATCA ACCAATCGTA ACCTTCATCT CTTCCACCCA TGTCTCTTTG AGCAATAAAG CCGATAACAA AATCTTTGTC GCTCTTCGCA ATGTCAACAG TACCCTTAGT ATATTCTCCA GTAGATAGGG AGCCCTTGCA TGACAATTCT GCTAACATCA AAAGGCCTCT AGGTTCCTTT GTTACTTCTT CTGCCGCCTG CTTCAAACCG CTAACAATAC CTGGGCCCAC CACACCGTGT GCATTCGTAA TGTCTGCCCA TTCTGCTATT CTGTATACAC CCGCAGAGTA CTGCAATTTG ACTGTATTAC CAATGTCAGC AAATTTTCTG TCTTCGAAGA GTAAAAAATT GTACTTGGCG GATAATGCCT TTAGCGGCTT AACTGTGCCC TCCATGGAAA AATCAGTCAA GATATCCACA TGTGTTTTTA GTAAACAAAT TTTGGGACCT AATGCTTCAA CTAACTCCAG TAATTCCTTG GTGGTACGAA CATCCAATGA AGCACACAAG TTTGTTTGCT TTTCGTGCAT GATATTAAAT AGCTTGGCAG CAACAGGACT AGGATGAGTA GCAGCACGTT CCTTATATGT AGCTTTCGAC ATGATTTATC TTCGTTTCCT GCAGGTTTTT GTTCTGTGCA GTTGGGTTAA GAATACTGGG CAATTTCATG TTTCTTCAAC ACTACATATG CGTATATATA CCAATCTAAG TCTGTGCTCC TTCCTTCGTT CTTCCTTCTG TTCGGAGATT ACCGAATCAA AAAAATTTCA AGGAAACCGA AATCAAAAAA AAGAATAAAA AAAAAATGAT GAATTGAA
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