pBT3-SUC
$99.00
In stock
SKU
PL77534
膜蛋白杂交
100μg liquid plasmid(Instantly):$120
| name | pBT3-SUC | another name | apBT3-SUC |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | CYC1 | Replicon | pBS |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCGCGGATGA TTACGCCAAG CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCATTTGGCG AGCGTTGGTT GGTGGATCAA GCCCACGCGT AGGCAATCCT CGAGCAGATC CGCCAGGCGT GTATATATAG CGTGGATGGC CAGGCAACTT TAGTGCTGAC ACATACAGGC ATATATATAT GTGTGCGACG ACACATGATC ATATGGCATG CATGTGCTCT GTATGTATAT AAAACTCTTG TTTTCTTCTT TTCTCTAAAT ATTCTTTCCT TATACATTAG GACCTTTGCA GCATAAATTA CTATACTTCT ATAGACACGC AAACACAAAT ACACACACTA ATCTAGATAT ATGATGCTTT TGCAAGCTTT CCTTTTCCTT TTGGCTGGTT TTGCAGCCAA AATATCTGCA ATGGCCATTA CGGCCAGGCC TTTAATTAAG GCCGCCTCGG CCATCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCATGTCG GGGGGGATCC CTCCAGATCA ACAAAGATTG ATCTTTGCCG GTAAGCAGCT AGAAGACGGT AGAACGCTGT CTGATTACAA CATTCAGAAG GAGTCCACCT TACATCTTGT GCTAAGGCTA AGAGGTGGTA TGCACAGATC AGCTTGCGGC CGCATGAAAG CGTTAACGGC CAGGCAACAA GAGGTGTTTG ATCTCATCCG TGATCACATC AGCCAGACAG GTATGCCGCC GACGCGTGCG GAAATCGCGC AGCGTTTGGG GTTCCGTTCC CCAAACGCGG CTGAAGAACA TCTGAAGGCG CTGGCACGCA AAGGCGTTAT TGAAATTGTT TCCGGCGCAT CACGCGGGAT CCGTCTGTTG CAGGAAGAGG AAGAAGGGTT GCCGCTGGTA GGTCGTGTGG CTGCCGGTGA ACCACTTCTG GCGCAACAGC ATATTGAAGG TCATTATCAG GTCGACCCTT CCTTATTCAA GCCGAATGCT GATTTCCTGC TGCGCGTCAG CGGGATGTCG ATGAAAGATA TCGGCATTAT GGATGGTGAC TTGCTGGCAG TGCATAAAAC TCAGGATGTA CGTAACGGTC AGGTCGTTGT CGCACGTATT GATGACGAGG TTACCGTTAA GCGCCTGAAA AAACAGGGCA ATAAAGTCGA ACTGTTGCCA GAAAATAGCG AGTTTAAACC AATTGTCGTA GATCTTCGTC AGCAGAGCTT CACCATTGAA GGGCTGGCGG TTGGGGTTAT TCGCAACGGC GACTGGCTGG AATTCCCGGG GATCTGGGCC CCCCCGACCG ATGTCAGCCT GGGGGACGAG CTCCACTTAG ACGGCGAGGA CGTGGCGATG GCGCATGCCG ACGCGCTAGA CGATTTCGAT CTGGACATGT TGGGGGACGG GGATTCCCCG GGTCCGGGAT TTACCCCCCA CGACTCCGCC CCCTACGGCG CTCTGGATAT GGCCGACTTC GAGTTTGAGC AGATGTTTAC CGATGCCCTT GGAATTGACG AGTACGGTGG GTAGGTCATG TAATTAGTTA TGTCACGCTT ACATTCACGC CCTCCCCCCA CATCCGCTCT AACCGAAAAG GAAGGAGTTA GACAACCTGA AGTCTAGGTC CCTATTTATT TTTTTATAGT TATGTTAGTA TTAAGAACGT TATTTATATT TCAAATTTTT CTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCGGCCGGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATCGACGG TCGAGGAGAA CTTCTAGTAT ATCCACATAC CTAATATTAT TGCCTTATTA AAAATGGAAT CCCAACAATT ACATCAAAAT CCACATTCTC TTCAAAATCA ATTGTCCTGT ACTTCCTTGT TCATGTGTGT TCAAAAACGT TATATTTATA GGATAATTAT ACTCTATTTC TCAACAAGTA ATTGGTTGTT TGGCCGAGCG GTCTAAGGCG CCTGATTCAA GAAATATCTT GACCGCAGTT AACTGTGGGA ATACTCAGGT ATCGTAAGAT GCAAGAGTTC GAATCTCTTA GCAACCATTA TTTTTTTCCT CAACATAACG AGAACACACA GGGGCGCTAT CGCACAGAAT CAAATTCGAT GACTGGAAAT TTTTTGTTAA TTTCAGAGGT CGCCTGACGC ATATACCTTT TTCAACTGAA AAATTGGGAG AAAAAGGAAA GGTGAGAGGC CGGAACCGGC TTTTCATATA GAATAGAGAA GCGTTCATGA CTAAATGCTT GCATCACAAT ACTTGAAGTT GACAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA GGATATACCA TTCTAATGTC TGCCCCTATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG GTGTCCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGTTGATGCC GTTTTGTTAG GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAGCTAATG TTTTGGCCTC TTCAAGATTA TGGAGAAAAA CTGTGGAGGA AACCATCAAG AACGAATTCC CTACATTGAA GGTTCAACAT CAATTGATTG ATTCTGCCGC CATGATCCTA GTTAAGAACC CAACCCACCT AAATGGTATT ATAATCACCA GCAACATGTT TGGTGATATC ATCTCCGATG AAGCCTCCGT TATCCCAGGT TCCTTGGGTT TGTTGCCATC TGCGTCCTTG GCCTCTTTGC CAGACAAGAA CACCGCATTT GGTTTGTACG AACCATGCCA CGGTTCTGCT CCAGATTTGC CAAAGAATAA GGTTGACCCT ATCGCCACTA TCTTGTCTGC TGCAATGATG TTGAAATTGT CATTGAACTT GCCTGAAGAA GGTAAGGCCA TTGAAGATGC AGTTAAAAAG GTTTTGGATG CAGGTATCAG AACTGGTGAT TTAGGTGGTT CCAACAGTAC CACCGAAGTC GGTGATGCTG TCGCCGAAGA AGTTAAGAAA ATCCTTGCTT AAAAAGATTC TCTTTTTTTA TGATATTTGT ACATAAACTT TATAAATGAA ATTCATAATA GAAACGACAC GAAATTACAA AATGGAATAT GTTCATAGGG TAGACGAAAC TATATACGCA ATCTACATAC ATTTATCAAG AAGGAGAAAA AGGAGGATAG TAAAGGAATA CAGGTAAGCA AATTGATACT AATGGCTCAA CGTGATAAGG AAAAAGAATT GCACTTTAAC ATTAATATTG ACAAGGAGGA GGGCACCACA CAAAAAGTTA GGTGTAACAG AAAATCATGA AACTACGATT CCTAATTTGA TATTGGAGGA TTTTCTCTAA AAAAAAAAAA ATACAACAAA TAAAAAACAC TCAATGACCT GACCATTTGA TGGAGTTTAA GTCAATACCT TCTTGAACCA TTTCCCATAA TGGTGAAAGT TCCCTCAAGA ATTTTACTCT GTCAGAAACG GCCTTACGAC GTAGTCGATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAC CACATGTGGA TGATCCAGTT CGATTTATTC AACAAAGCCG CCGTCCCGTC AAGTCAGCGT AATGCTCTGC CAGTGTTACA ACCAATTAAC CAATTCTGAT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTCGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGATGAT ATATTTTTAT CTTGTGCAAT GTAACATCAG AGATTTTGAG ACACAACGTG GCTTACTAGG ATCGATCCGA CATGTGGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CTCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAG
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