pYL91
$99.00
In stock
SKU
PL77486
含部分DMD基因片段
100μg liquid plasmid(Instantly):$120
| name | pYL91 | another name | apYL91 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGA ATTCCAACAT CCAATAAATC ATACAGGCAA GGCAAAGAAT TAGCAAAATT AAGCAATAAA GCCTCAGAGC ATAAAGCTAA ATCGGTTGTA CCAAAAACAT TATGACCCTG TAATACTTTT GCGGGAGAAG CCTTTATTTC AACGCAAGGA TAAAAATTTT TAGAACCCTC ATATATTTTA AATGCAATGC CTGAGTAATG TGTAGGTAAA GATTCAAACG GGTGAGAAAG GCCGGAGACA GTCAAATCAC CATCAATATG ATATTCAACC GTTCTAGCTG ATAAATTCAT GCCGGAGAGG GTAGCTATTT TTGAGAGGTC TCTACAAAGG CTATCAGGTC ATTGCCTGAG AGTCTGGAGC AAACAAGAGA ATCGATGAAC GGTAATCGTA AAACTAGCAT GTCAATCATA TGTACCCCGG TTGATAATCA GAAAAGCCCC AAAAACAGGA AGATTGTATA AGCAAATATT TAAATTGTAA ACGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTACT ATGGTTGCTT TGACGAGCAC GTATAACGTG CTTTCCTCGT TAGAATCAGA GCGGGAGCTA AACAGGAGGC CGATTAAAGG GATTTTAGAC AGGAACGGTA CGCCAGAATC CTGAGAAGTG TTTTTATAAT CAGTGAGGCC ACCGAGTAAA AGAGTCTGTC CATCACGCAA ATTAACCGTT GTCGCAATAC TTCTTTGATT AGTAATAACA TCACTTGCCT GAGTAGAAGA ACTCAAACTA TCGGCCTTGC TGGTAATATC CAGAACAATA TTACCGCCAG CCATTGCAAC AGGAAAAACG CTCATGGAAA TACCTACATT TTGACGCTCA ATCGTCTGGA ATTCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGC GCTCGCTCGC TCACTGAGGC CGCCCGGGCA AAGCCCGGGC GTCGGGCGAC CTTTGGTCGC CCGGCCTCAG TGAGCGAGCG AGCGCGCAGA GAGGGAGTGG CCAACTCCAT CACTAGGGGT TCCTTGTAGT TAATGATTAA CCCGCCATGC TACTTATCTA CGGCCGCGGT ACCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCTA GACGGCCGCG GTTTTTTTTA TCGCTGCCTT GATATACACT TTCCACCATG CTTTGGTGGG AAGAAGTAGA GGACTGTTAT GAAAGAGAAG ATGTTCAAAA GAAAACATTC ACAAAATGGG TAAATGCACA ATTTTCTAAG TTTGGGAAGC AGCATATTGA GAACCTCTTC AGTGACCTAC AGGATGGGAG GCGCCTCCTA GACCTCCTCG AAGGCCTGAC AGGGCAAAAA CTGCCAAAAG AAAAAGGATC CACAAGAGTT CATGCCCTGA ACAATGTCAA CAAGGCACTG CGGGTTTTGC AGAACAATAA TGTTGATTTA GTGAATATTG GAAGTACTGA CATCGTAGAT GGAAATCATA AACTGACTCT TGGTTTGATT TGGAATATAA TCCTCCACTG GCAGGTCAAA AATGTAATGA AAAATATCAT GGCTGGATTG CAACAAACCA ACAGTGAAAA GATTCTCCTG AGCTGGGTCC GACAATCAAC TCGTAATTAT CCACAGGTTA ATGTAATCAA CTTCACCACC AGCTGGTCTG ATGGCCTGGC TTTGAATGCT CTCATCCATA GTCATAGGCC AGACCTATTT GACTGGAATA GTGTGGTTTG CCAGCAGTCA GCCACACAAC GACTGGAACA TGCATTCAAC ATCGCCAGAT ATCAATTAGG CATAGAGAAA CTACTCGATC CTGAAGATGT TGATACCACC TATCCAGATA AGAAGTCCAT CTTAATGTAC ATCACATCAC TCTTCCAAGT TTTGCCTCAA CAAGTGAGCA TTGAAGCCAT CCAGGAAGTG GAAATGTTGC CAAGGCCACC TAAAGTGACT AAAGAAGAAC ATTTTCAGTT ACATCATCAA ATGCACTATT CTCAACAGAT CACGGTCAGT CTAGCACAGG GATATGAGAG AACTTCTTCC CCTAAGCCTC GATTCAAGAG CTATGCCTAC ACACAGGCTG CTTATGTCAC CACCTCTGAC CCTACACGGA GCCCATTTCC TTCACAGCAT TTGGAAGCTC CTGAAGACAA GTCATTTGGC AGTTCATTGA TGGAGAGTGA AGTAAACCTG GACCGTTATC AAACAGCTTT AGAAGAAGTA TTATCGTGGC TTCTTTCTGC TGAGGACACA TTGCAAGCAC AAGGAGAGAT TTCTAATGAT GTGGAAGTGG TGAAAGACCA GTTTCATACT CATGAGGGGT ACATGATGGA TTTGACAGCC CATCAGGGCC GGGTTGGTAA TATTCTACAA TTGGGAAGTA AGCTGATTGG AACAGGAAAA TTATCAGAAG ATGAAGAAAC TGAAGTACAA GAGCAGATGA ATCTCCTAAA TTCAAGATGG GAATGCCTCA GGGTAGCTAG CATGGAAAAA CAAAGCAATT TACATAGAGT TTTAATGGAT CTCCAGAATC AGAAACTGAA AGAGTTGAAT GACTGGCTAA CAAAAACAGA AGAAAGAACA AGGAAAATGG AGGAAGAGCC TCTTGGACCT GATCTTGAAG ACCTAAAACG CCAAGTACAA CAACATAAGG TGCTTCAAGA AGATCTAGAA CAAGAACAAG TCAGGGTCAA TTCTCTCACT CACATGGTGG TGGTAGTTGA TGAATCTAGT GGAGATCACG CAACTGCTGC TTTGGAAGAA CAACTTAAGG TATTGGGAGA TCGATGGGCA AACATCTGTA GATGGACAGA AGACCGCTGG GTTCTTTTAC AAGACGAAAT TTCTTATGTG CCTTCTACTT ATTTGACTGA AATCACTCAT GTCTCACAAG CCCTATTAGA AGTGGAACAA CTTCTCAATG CTCCTGACCT CTGTGCTAAG GACTTTGAAG ATCTCTTTAA GCAAGAGGAG TCTCTGAAGA ATATAAAAGA TAGTCTACAA CAAAGCTCAG GTCGGATTGA CATTATTCAT AGCAAGAAGA CAGCAGCATT GCAAAGTGCA ACGCCTGTGG AAAGGGTGAA GCTACAGGAA GCTCTCTCCC AGCTTGATTT CCAATGGGAA AAAGTTAACA AAATGTACAA GGACCGACAA GGGCGATTTG ACAGATCTGT TGAGAAATGG CGGCGTTTTC ATTATGATAT AAAGATATTT AATCAGTGGC TAACAGAAGC TGAACAGTTT CTCAGAAAGA CACAAATTCC TGAGAATTGG GAACATGCTA AATACAAATG GTATCTTAAG GAACTCCAGG ATGGCATTGG GCAGCGGCAA ACTGTTGTCA GAACATTGAA TGCAACTGGG GAAGAAATAA TTCAGCAATC CTCAAAAACA GATGCCAGTA TTCTACAGGA AAAATTGGGA AGCCTGAATC TGCGGTGGCA GGAGGTCTGC AAACAGCTGT CAGACAGAAA AAAGAGGCTA GAAGAAACCC TTGAAAGACT CCAGGAACTT CAAGAGGCCA CGGATGAGCT GGACCTCAAG CTGCGCCAAG CTGAGGTGAT CAAGGGATCC TGGCAGCCCG TGGGCGATCT CCTCATTGAC TCTCTCCAAG ATCACCTCGA GAAAGTCAAG GCACTTCGAG GAGAAATTGC GCCTCTGAAA GAGAACGTGA GCCACGTCAA TGACCTTGCT CGCCAGCTTA CCACTTTGGG CATTCAGCTC TCACCGTATA ACCTCAGCAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG GCCGTCGAGG ACCGAGTCAG GCAGCTGCAT GAAGCCCACA GGGACTTTGG TCCAGCATCT CAGCACTTTC TTTCCACGTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC GCCAAACAAA GTGCCCTACT ATATCAACCA CGAGACTCAA ACAACTTGCT GGGACCATCC CAAAATGACA GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGAT TCTCAGCTTA TAGGACTGCC ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT GTCAGCTGCA TGTGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG ATTATTAATT GTTTGACCAC TATTTATGAC CGCCTGGAGC AAGAGCACAA CAATTTGGTC AACGTCCCTC TCTGCGTGGA TATGTGTCTG AACTGGCTGC TGAATGTTTA TGATACGGGA CGAACAGGGA GGATCCGTGT CCTGTCTTTT AAAACTGGCA TCATTTCCCT GTGTAAAGCA CATTTGGAAG ACAAGTACAG ATACCTTTTC AAGCAAGTGG CAAGTTCAAC AGGATTTTGT GACCAGCGCA GGCTGGGCCT CCTTCTGCAT GATTCTATCC AAATTCCAAG ACAGTTGGGT GAAGTTGCAT CCTTTGGGGG CAGTAACATT GAGCCAAGTG TCCGGAGCTG CTTCCAATTT GCTAATAATA AGCCAGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGAG ACTGGAACCC CAGTCCATGG TGTGGCTGCC CGTCCTGCAC AGAGTGGCTG CTGCAGAAAC TGCCAAGCAT CAGGCCAAAT GTAACATCTG CAAAGAGTGT CCAATCATTG GATTCAGGTA CAGGAGTCTA AAGCACTTTA ATTATGACAT CTGCCAAAGC TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC CATAAAATGC ACTATCCCAT GGTGGAATAT TGCACTCCGA CTACATCAGG AGAAGATGTT CGAGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTGAC ACAATGTAGG AAGTCTTTTC CACATGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TATCAGTCAT GACAGATGAA GAAGGAGCAG AATAAATGTT TTACAACTCC TGATTCCCGC ATGCGGCCGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTGCGGCC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCTG CTG
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