pTSU2-APP
$99.00
In stock
SKU
PL77387
100μg liquid plasmid(Instantly):$120
| name | pTSU2-APP | another name | apTSU2-APP |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATTTCTCAGT TTCAGTTTCA TTTTTCTTGT TCTATTACAA CTTTTTTTAC TTCTTGCTCA TTAGAAAGAA AGCATAGCAA TCTAATCTAA GTTTTCTAGA TATATGATGC TTTTGCAAGC TTTCCTTTTC CTTTTGGCTG GTTTTGCAGC CAAAATATCT GCAATGGCCA TTACGGCCCT GGAGGTACCC ACTGATGGTA ATGCTGGCCT GCTGGCTGAA CCCCAGATTG CCATGTTCTG TGGCAGACTG AACATGCACA TGAATGTCCA GAATGGGAAG TGGGATTCAG ATCCATCAGG GACCAAAACC TGCATTGATA CCAAGGAAGG CATCCTGCAG TATTGCCAAG AAGTCTACCC TGAACTGCAG ATCACCAATG TGGTAGAAGC CAACCAACCA GTGACCATCC AGAACTGGTG CAAGCGGGGC CGCAAGCAGT GCAAGACCCA TCCCCACTTT GTGATTCCCT ACCGCTGCTT AGTTGGTGAG TTTGTAAGTG ATGCCCTTCT CGTTCCTGAC AAGTGCAAAT TCTTACACCA GGAGAGGATG GATGTTTGCG AAACTCATCT TCACTGGCAC ACCGTCGCCA AAGAGACATG CAGTGAGAAG AGTACCAACT TGCATGACTA CGGCATGTTG CTGCCCTGCG GAATTGACAA GTTCCGAGGG GTAGAGGTTT GTGTGTTGCC CACTGGCTGA AGAAAGTGAC AATGTGGATT CTGCTGATGC GGAGGAGGAG GACTCGGATG TCTGGGGGGG GCGGAGCAGA CACAGACTAT GCAGATGGGA GTGAAGACAA AGTAGTAGAA GTAGCAGAGG AGGAAGAAGT GGCTGAGGTG GAAGAAGAAG AAGCCGATGA TGACGAGGAC GATGAGGATG GTGATGAGGT AGAGGAAGAG GCTGAGGAAC CCTACGAAGA AGCCACAGAG AGAACCACCA GCATTGCCAC CACCACCACC ACCACCACAG AGTCTGTGGA AGAGGTGGTT CGAGTTCCTA CAACAGCAGC CAGTACCCCT GATGCCGTTG ACAAGTATCT CGAGACACCT GGGGATGAGA ATGAACATGC CCATTTCCAG AAAGCCAAAG AGAGGCTTGA GGCCAAGCAC CGAGAGAGAA TGTCCCAGGT CATGAGAGAA TGGGAAGAGG CAGAACGTCA AGCAAAGAAC TTGCCTAAAG CTGATAAGAA GGCAGTTATC CAGCATTTCC AGGAGAAAGT GGAATCTTTG GAACAGGAAG CAGCCAACGA GAGACAGCAG CTGGTGGAGA CACACATGGC CAGAGTGGAA GCCATGCTCA ATGACCGCCG CCGCCTGGCC CTGGAGAACT ACATCACCGC TCTGCAGGCG GTTCCTCCTC GGCCTCGTCA CGTGTTCAAT ATGCTAAAGA AGTATGTCCG CGCAGAACAG AAGGACAGAC AGCACACCCT AAAGCATTTC GAGCATGTGC GCATGGTGGA TCCCAAGAAA GCCGCTCAGA TCCGGTCCCA GGTTATGACA CACCTCCGTG TGATTTATGA GCGCATGAAT CAGTCTCTCT CCCTGCTCTA CAACGTGCCT GCAGTGGCCG AGGAGATTCA GGATGAAGTT GATGAGCTGC TTCAGAAAGA GCAAAACTAT TCAGATGACG TCTTGGCCAA CATGATTAGT GAACCAAGGA TCAGTTACGG AAACGATGCT CTCATGCCAT CTTTGACCGA AACGAAAACC ACCGTGGAGC TCCTTCCCGT GAATGGAGAG TTCAGCCTGG ACGATCTCCA GCGTGGCATT CTTTTGGGGC TGACTCTGTG CCAGCCAACA CAGAAAACGA AGTTGAGCCT GTTGATGCCC GCCCTGCTGC CGACCGAGGA CTGACCACTC GACCAGGTTC TGGGTTGACA AATATCAAGA CGGAGGAGAT CTCTGAAGTG AAGATGGATG CAGAATTCCG ACATGACTCA GGATATGAAG TTCATCATCA AAAATTGGTG TTCTTTGCAG AAGATGTGGG TTCAAACAAA GGTGCAATCA TTGGACTCAT GGTGGGCGGT GTTGTCATAG CGACAGTGAT CGTCATCACC TTGGTGATGC TGAAGAAGAA ACAGTACACA TCCATTCATC ATGGTGTGGT GGAGGTTGAC GCCGCTGTCA CCCCAGAGGA GCGCCACCTG TCCAAGATGC AGCAGAACGG CTACGAAAAT CCAACCTACA AGTTCTTTGA GCAGATGCAG AACCCGGCCG CCTCGGCCAT CTGCAGGAAT TCGATATCAA GCTTATCGAT ACCGTCGACC ATGTCGGGGG GGATCCCTCC AGATCAACAA AGATTGATCT TTGCCGGTAA GCAGCTAGAA GACGGTAGAA CGCTGTCTGA TTACAACATT CAGAAGGAGT CCACCTTACA TCTTGTGCTA AGGCTAAGAG GTGGTATGCA CAGATCAGCT TGCGGCCGCA TGAAAGCGTT AACGGCCAGG CAACAAGAGG TGTTTGATCT CATCCGTGAT CACATCAGCC AGACAGGTAT GCCGCCGACG CGTGCGGAAA TCGCGCAGCG TTTGGGGTTC CGTTCCCCAA ACGCGGCTGA AGAACATCTG AAGGCGCTGG CACGCAAAGG CGTTATTGAA ATTGTTTCCG GCGCATCACG CGGGATCCGT CTGTTGCAGG AAGAGGAAGA AGGGTTGCCG CTGGTAGGTC GTGTGGCTGC CGGTGAACCA CTTCTGGCGC AACAGCATAT TGAAGGTCAT TATCAGGTCG ACCCTTCCTT ATTCAAGCCG AATGCTGATT TCCTGCTGCG CGTCAGCGGG ATGTCGATGA AAGATATCGG CATTATGGAT GGTGACTTGC TGGCAGTGCA TAAAACTCAG GATGTACGTA ACGGTCAGGT CGTTGTCGCA CGTATTGATG ACGAAGTTAC CGTTAAGGGC CTGAAAAAAC AGGGCAATAA AGTCGAACTG TTGCCAGAAA ATAGCGAGTT TAAACCAATT GTCGTAGATC TTCGTCAGCA GAGCTTCACC ATTGAAGGGC TGGCGGTTGG GGTTATTCGC AACGGCGACT GGCTGGAATT CCCGGGGATC TGGGCCCCCC CGACCGATGT CAGCCTGGGG GACGAGCTCC ACTTAGACGG CGAGGACGTG GCGATGGCGC ATGCCGACGC GCTAGACGAT TTCGATCTGG ACATGTTGGG GGACGGGGAT TCCCCGGGTC CGGGATTTAC CCCCCACGAC TCCGCCCCCT ACGGCGCTCT GGATATGGCC GACTTCGAGT TTGAGCAGAT GTTTACCGAT GCCCTTGGAA TTGACGAGTA CGGTGGGTAG GTCATGTAAT TAGTTATTCA CGCTACAACC CCC
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