pLVX-TetOne-P210
$171.00
In stock
SKU
PL77258
100μg liquid plasmid(Instantly):$192
| name | pLVX-TetOne-P210 | another name | apLVX-TetOne-P210 |
| Insert Size (bp) | 6096bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLVX-TetOne-Puro | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGACTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAGGCAATAG CATCACAAAT TTCACAAATA AGGCATTTTT TTCACTGCAT TCTAGTTTTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CTCAAATCCC TCGGAAGCTG CGCCTGTCTT AGGTTGGAGT GATACATTTT TATCACTTTT ACCCGTCTTT GGATTAGGCA GTAGCTCTGA CGGCCCTCCT GTCTTAGGTT AGTGAAAAAT GTCACTCTCT TACCCGTCAT TGGCTGTCCA GCTTAGCTCG CAGGGGAGGT GGTCTGGATC CGCCGGCACC GGTCTACCTC TGCACTATGT CACTGATTTC CTTCACCGAA CTGAGGAGCT TGCTGAAGTC CTGAGTGGCC GCTGGACCAC TGCCTGCTGT CGCCGGGCAG ATCTGAAGCT CCCGGAGATT ATTCTCCAGT TTGTTGATGG CCTCTCGGAA GGCAAACTTG TTCCTCATTT GCTGGATGGA ATCCACATAG CTCACGCAGA ACGTGTAGAG GTTTTTGCCG GCCTCCAGCA CTGCGCTGTG GCTGGCCATC TGCTCGGAGT TCCTAGAGAT GGCGAGGCAC AGCGCCTCGG TGCTGTCCAG GACCACGCCC TTGGTGATGG CGCCGCTGGC GATCCGCTCT GGAGGCTGGC GGGTTTTCCG AAGAGACACT CGGGTTGATA TGAGAGGGAT GAAGGCGGTG GAAGACGGCT GGTCCCCTGC CAGGGCCGAG GATGCTGATG GCAACGTGGA GGGAACGGGG GCTGGGCTGA TGGGGGTCCC CGACGGCTTG GCGGACTGTG GCTTTGGAGT GGCCGGGAGC ACGGGCTTTT TGAGGCCCTC TCCCGGCTGG CTGGGCTTGG CAGCGTCACT GTTCACAGCA TCAACCAGAC TCGTGGCTTT TGTCTTTGCG CCCAGGACTG CCTCCCCGGC CGCCTCCTGG CTCGGGCTCT GCGAGGGCTT TCCTCCAGCC TTCCCTGCAG AGGCTGCTGG TGGGGGCGGC GGGGCAGGTT TGAGCCTGGA CAATTTCCCC TTGTCCCTCC CTGGCGACTC AGAGGAGTGC TTGTGCCTCC TCACTCTGGA CTCCTCGGCG GGGCCCTTGC TGGTGCCCCC TGGTGCACCT GAGCCTGCTT TGCTGGTGGG GGTCACTGGC TCAGCTGCAG CAGGGGTCCC TAAGGCACTG CCCTTTCCAG CTTCTTCCTT GTGGGGGAGG CCCGAGGCAG GGGCCACGGT GACCTGCCGC CGGAGGGGTT TTGGAGTCAG GTTGGGCGGG CTGGAGCCCG GGCTGGACTC CATGATGTCT TTGAAGACCT CATCAGCAGC TTCCTCATTC TTTTTCACCA GCCTGGGGGG AGGCGTTACT GTGCCTCGGG TCACCTGGTC AGACCTGTTC TCCCCTGCCC TCTTCCGAGG CAGAGCCGGC TTCTCACTTT TGTGCCCTCC AAATGTGGAC GAGTCAAACT GTCTTCCCGT GGACTGCAAG TCCCGAGGCA GCGTGACTGA CCTCCACTCC GTGTCCTTGG CCCCATGGGG AACGCAGGAG GCGGAGCAAG AGCGCAGGAA GCGCTTGCTG GAGCTGCCAC CGCCCTCCTC CTCGCCGGTG GCTAGGCGGC TGCTGGTCAG CGTGCTGGAC TTCTTCCACA GGTGGGGAGA CCGGAAGCCT GAGCCCCCAG ACTCCCGGAG GGCTCCATTG GGGACCCCAG CCCCATTGCT GGGCTTTGGG GACTTGGCTG GGTCAGCTGT GTCCAAGGGG GTGAAAGCCA GTGCCCCGTT GCTGATGTCT CGGCCCTCTT CCTCGCCGGC CCCTCTGCGC TCCGGCTGGC CGTCCATCTC CCGGAAGGAG CTGCTGCGTT TGGGAGGGGT TGGGGCTGTC TTCTTCTTCT TCTTGATCAA GGCGCTGAAC AAGTTGGTCT TTTTGTCTTT GGGGAGAAGG CGCTCATCTT CATTCAGGCC GCCCTCCGGG GGACCTCGCT CTTTTCGAGG GAGCAATGGA GACACGGCAG GCTCATGGTC CAGAGGATCG CTCTCTCCCT GGCCCTTGGA GTGAGGCATC TCAGGCACGT CAGTGGTGTC TCTGTGCTCT GCAGCTCTCC TGGAGGTCCT CGTCTTGGTG GGCAGCTCTG GGGCCTGCAG CAAGGTACTC ACAGCCCCAC GGACGCCTTG TTTCCCCAGC TCCTTTTCCA CTTCGTCTGA GATACTGGAT TCCTGGAACA TTGTTTCAAA GGCTTGGTGG ATTTCAGCAA AGGAGGGCCG GTCAGAGGGA TTCCACTGCC AACATGCTCG CATGAGTTCA TAGACCTTCT CTGGGCAGCC TTCTGGGCGC TCCATGCGGT AGTCCTTCTC TAGCAGCTCA TACACCTGGG ACAGGTCAAT TCCCGGGTAA GGGGACATGC CATAGGTAGC AATTTCCCAA AGCAATACTC CAAATGCCCA GACGTCGGAC TTGATGGAGA ACTTGTTGTA GGCCAGGCTC TCGGGTGCAG TCCATTTGAT GGGGAACTTG GCTCCAGCAT GGGCTGTGTA GGTGTCCCCT GTCATCAACC TGCTCAGGCC AAAATCAGCT ACCTTCACCA AGTGGTTCTC CCCTACCAGG CAGTTTCGGG CAGCAAGATC TCTGTGGATG AAGTTTTTCT TCTCCAGGTA CTCCATGGCT GACGAGATCT GAGTGGCCAT GTACAGCAGC ACCACGGCGT TCACCTCCTG CCGGTTGCAC TCCCTCAGGT AGTCCAGGAG GTTCCCGTAG GTCATGAACT CAGTGATGAT ATAGAACGGG GGCTCCCGGG TGCAGACCCC AAGGAGCTGC ACCAGGTTAG GGTGTTTGAT CTCTTTCATG ACTGCAGCTT CTTTCAAGAA CTCTTCCACC TCCATGGTGT CCTCCTTCAA GGTCTTCACG GCCACCGTCA GGCTGTATTT CTTCCACACG CCCTCGTACA CCTCCCCGTA CTGGCCCCCG CCCAGCTTGT GCTTCATGGT GATGTCCGTG CGTTCCATCT CCCACTTGTC GTAGTTGGGG GACACACCAT AGACAGTGGG CTTGTTGCGC TTTGGGGCTG GATAATGGAG CGTGGTGATG AGCCCGTCGG CCACCGTTGA ATGATGATGA ACCAACTCGG CCAGGGTGTT GAAGCGGCTC TCGGAGGAGA CGTAGAGCTT GCCATCAGAA GCAGTGTTGA TCCTGTAATG GTACACCCTC CCTTCGTATC TCAGCGAGAT GGACCTCTGG CCAGGACTGC TCTCACTCTC ACGCACCAAG AAGCTGCCAT TGATCCCGCT GCTCAGCAGA TACTCAGCGG CATTGCGGGA CACAGGCCCA TGGTACCAGG AGTGTTTCTC CAGACTGTTG ACTGGCGTGA TGTAGTTGCT TGGGACCCAG CCTTGGCCAT TTTTGGTTTG GGCTTCACAC CATTCCCCAT TGTGATTATA GCCTAAGACC CGGAGCTTTT CACCTTTAGT TATGCTTAGA GTGTTATCTC CACTGGCCAC AAAATCATAC AGTGCAACGA AAAGGTTGGG GTCATTTTCA CTGGGTCCAG CGAGAAGGTT TTCCTTGGAG TTCCAACGAG CGGCTTCACT CAGACCCTGA GGCTCAAAGT CAGATGCTAC TGGCCGCTGA AGGGCTTTTG AACTCTGCTT AAATCCAGTG GCTGAGTGGA CGATGACATT CAGAAACCCA TAGAGCCCCG GAGACTCATC ATCTTCCTTA TTGATGGTCA GCGGAATGCT GTGGACAGTC TGGAGTTTCA CACACGAGTT GGTCAGCATC TGCAGCTCCA CGGATGTCAG GGAGAAGCTT CTGAAACACT TCTTCTGCTG CTCCCGGATG TTCTCCCTCC ACTCTGCACG CTCATAGTCA GAGGAGATCA GGAACGTGTA ACTCTTGCCG TTGCGGCTGT GCACCCTGAA GGCCATGCTG GGAGACATAA GCAGCAGCAG TGACTCCTGC TCCGACAGCT TCTTCTTCAG CCTCTCCGTA GCCTTGCTGC CCTTGTTCGC CCTCTTCTCT CTCTGGATGT CACTCTTGAT CTGGGAGATC TTGATCTTCA AAGCGTCCAG CTCCTCATCG GGCACCAGGG GGATGTTGGG CACTGCCTCC AGTTCATCCA CCATCTGGAA GCTGAGATCC GTGAGCGGAA TGTACCATTT GCAGTCATAC TGCTGCGTTT TGCCTCCGCT CTGCTTCTTG AGCTTGGTGC AGAGAAGCAG GTCGGTGAAC AGGAAGACGT GGCGCAGCTT GCGGGCCCCC TCCACCAGCT CCACCATGAA GCTGTCCTTC AGCAGCTGCC GGTGCTCTCC CTTCTTCACC GTCATGGACT GCCGTCGGGG TGTGATCTCC TCATTGATGC TGGACAGGAA GTTCTGTGAG ATGCGGAGGG CGTCCTGCAG CAAGGGGTGG TCAGGGTGGC TGGCAGGAGT GTGCTTCAGC AAGTCATGGA GGACCAGCGT GCTCCTCGTC ACACGGTCCA CAGGCTTGTA GAGCAGAGTT TCCAGAGAGT TCTTGGTCGT TGGATCCTTG GCATCTTTGT TGCTTCTGGC TCTCAGGTTC TCGGAGATTT CTGCAAACTG AGCATTGGCC TGACAGCACT TCTCAGCCAT TTCCATGGCA ACTCCGTAGT TGTCCACGAA GGCCCGGTAC ACACCCAGCT GGCTGGCCAG CTTCTGGAAG AGGTCGCCCA CCCGCTGCTG GTGGCTCCAC TGCTGCACGC GGGGGAAGAG CCCATCATAG AACTCCTTGT GGATCTCGTA GAGCTCAGGC ACTTTGAAGA AGATGGTCTC GATCTGCTGA CTCGTCAGCA CCGGCTGAGA GGTGGTGGCA GCGGCTTTCA AAGGCTTCAT GGGCAGCAGC AGTGCCTCCA GGTGGCTCAG GTAAGTCTCC TCGCTAGCCA GGATTCCCGA CAGGACCCAT TTTCTCATCT CCAAGCCCTT TTCCAAGTCC AGCTCACTCG CTTTAGTGGA CTCCAGGGCT CCCGAGACCA GCGCATCCCG GCTGCCCCTG CCCTTGCTGC TGAGGTGGGG CGATGAGGAG GGCGAGTCAT CAATGTAGGG CAGCCCATCT TGGTGCCGGC GCTGCTCCTC TGCCCGGTCT GCAGCGCAGC CGTATCCAGG TGGTGTTCCG AACGAGCCAT CTGCGTCTCC ATGGAAGGCG CCCTCGCCAT CGTTGGGCCA GATCTGCCCG GTCTTGCGGA CGCCCACGAT GGTGGCCTCG GACACGACAA CCGGGCAGTG CCGGTGCCGC TTATGGCACT GCGGCGTGGG GGGACTGCTG CTGTCGAAGG ACTGTTGCGA GTTCTGCGAG GGAGAGCGGC TTTTGTCCCG GAACATGCGG TAGGTGGTGG GGCTTGGGGA CACGCGGCTG GACTGGCCAG AGGAGAAGTC CTCCTCGCTG GAGGTGAGGT TCTCATTGGA GCTGCAGTCC GGGGTATAGC CGCCTCCGCA ATCCTCAAAA CTCCGGGGGG AGTAGGACCT GCGGGGCCAG GTAAGGCGCT TCTCCTGCTC AGAGGTGCTC TGGCTGCGCA GGAGCGGGCC CTTGCCCTCC CCTTCCATCA TGCCCCCGAC GTAGATGCTC TGGTAGGGCT GGTACTCCAG GGGCGGCCAA GGGGGCCTGC TACCGCCATT GGCGTCGATC AGGTTGTCCT TCAGGAAGCG GGGGTTCAAC TCGGCGTCCT CGTAGTCGCC GTCGACGCCG CAGCTGCTCT CCGAGGAGCG TCCCCGGTAA GGGGGCCTGG ATGCATCCCC CACGCTCGAG CCCGCGCCGT GCTGGGACTT TTTGCGCTCC ATCTGCATGG CCTGGCTGCC CAGGGAGCTG ATGCGGTCCG ACACCTCTTT GTCGTTGACC TTCACCAGGC CGCGCTCGTG GTGAAACTCG ACGTTCACGT AGAAGGGCTT CTCGGCGTCC GCCCCGGGCT GGCCATGGCC CTTGCGTATC CGCTCGAAGT TGGACCTGAG CGCCGCCACG CTGGCGGGGG GTCCCCGGTC GTCCCGTTCC CCCGACGCGG CTGCCCCGGG CCTGCGGGCG GTCCCGGGCC TGGCCTTACC CGGAGAACCC TCGCCGTCGG GCCGGGCCTC GGGCTCCTCG GCGGGCGGCG GGTCGGCTCC GTCGGCGGGC GCTGGCTGCG GGCGCGACGC GGACGCTCGG GGCTCGGAGG CGCCGTCGGG GGCCTGCGCC GCGCGCCGGA AGCCCCATCG CTGCCGGTCA TAGCTCTTCT TTTCCTTGGC CAGCAACGTC TGCAGGTAGA TCATGCGGAA GCGCTCCTGG TTCACCTCCT GCTCCAGGCG CCGAATGGAG GCCTTGCAGC GCTCCAGCTC CTGCTCGATG TCGCCCACTG AGCGCAGCTC CATGCGCGGG GGCTCTGAGT CCGGGAACTG CGCCTTCCAC GCCTCCGCGA AGCCCACCGG GTCCACCATG AATTCTTTAC GAGGGTAGGA AGTGGTACGG AAAGTTGGTA TAAGACAAAA GTGTTGTGGA ATTGAAGTTT ACTCAAAAAA TCAGCACTCT TTTATAGGCG CCCTGGTTTA CATAAGCAAA GCTTATACGT TCTCTATCAC TGATAGGGAG TAAACTGGAT ATACGTTCTC TATCACTGAT AGGGAGTAAA CTGTAGATAC GTTCTCTATC ACTGATAGGG AGTAAACTGG TCATACGTTC TCTATCACTG ATAGGGAGTA AACTCCTTAT ACGTTCTCTA TCACTGATAG GGAGTAAAGT CTGCATACGT TCTCTATCAC TGATAGGGAG TAAACTCTTC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGAGGTGA TAATTCCACG GGGTTGGGGT TGCGCCTTTT CCAAGGCAGC CCTGGGTTTG CGCAGGGACG CGGCTGCTCT GGGCGTGGTT CCGGGAAACG CAGCGGCGCC GACCCTGGGT CTCGCACATT CTTCACGTCC GTTCGCAGCG TCACCCGGAT CTTCGCCGCT ACCCTTGTGG GCCCCCCGGC GACGCTTCCT GCTCCGCCCC TAAGTCGGGA AGGTTCCTTG CGGTTCGCGG CGTGCCGGAC GTGACAAACG GAAGCCGCAC GTCTCACTAG TACCCTCGCA GACGGACAGC GCCAGGGAGC AATGGCAGCG CGCCGACCGC GATGGGCTGT GGCCAATAGC GGCTGCTCAG CAGGGCGCGC CGAGAGCAGC GGCCGGGAAG GGGCGGTGCG GGAGGCGGGG TGTGGGGCGG TAGTGTGGGC CCTGTTCCTG CCCGCGCGGT GTTCCGCATT CTGCAAGCCT CCGGAGCGCA CGTCGGCAGT CGGCTCCCTC GTTGACCGAA TCACCGACCT CTCTCCCCAG GGGGATCATC GAATTACCAT GTCTAGACTG GACAAGAGCA AAGTCATAAA CTCTGCTCTG GAATTACTCA ATGGAGTCGG TATCGAAGGC CTGACGACAA GGAAACTCGC TCAAAAGCTG GGAGTTGAGC AGCCTACCCT GTACTGGCAC GTGAAGAACA AGCGGGCCCT GCTCGATGCC CTGCCAATCG AGATGCTGGA CAGGCATCAT ACCCACTCCT GCCCCCTGGA AGGCGAGTCA TGGCAAGACT TTCTGCGGAA CAACGCCAAG TCATACCGCT GTGCTCTCCT CTCACATCGC GACGGGGCTA AAGTGCATCT CGGCACCCGC CCAACAGAGA AACAGTACGA AACCCTGGAA AATCAGCTCG CGTTCCTGTG TCAGCAAGGC TTCTCCCTGG AGAACGCACT GTACGCTCTG TCCGCCGTGG GCCACTTTAC ACTGGGCTGC GTATTGGAGG AACAGGAGCA TCAAGTAGCA AAAGAGGAAA GAGAGACACC TACCACCGAT TCTATGCCCC CACTTCTGAA ACAAGCAATT GAGCTGTTCG ACCGGCAGGG AGCCGAACCT GCCTTCCTTT TCGGCCTGGA ACTAATCATA TGTGGCCTGG AGAAACAGCT AAAGTGCGAA AGCGGCGGGC CGACCGACGC CCTTGACGAT TTTGACTTAG ACATGCTCCC AGCCGATGCC CTTGACGACT TTGACCTTGA TATGCTGCCT GCTGACGCTC TTGACGATTT TGACCTTGAC ATGCTCCCCG GGTAAACGCG CGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAACGCG ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAACGCGTC TGGAACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA ATTAATTCTG CAGTCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGAGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTAGCAGAA CTACACACCA GGGCCAGGGG TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CCAGATAAGG TAGAAGAGGC CAATAAAGGA GAGAACACCA GCTTGTTACA CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTGTTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACGTGGCCCG AGAGCTGCAT CCGGAGTACT TCAAGAACTG CTGATATCGA GCTTGCTACA AGGGACTTTC CGCTGGGGAC TTTCCAGGGA GGCGTGGCCT GGGCGGGACT GGGGAGTGGC GAGCCCTCAG ATCCTGCATA TAAGCAGCTG CTTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGCCT TGACATTGCT AGCGTTTTAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC AACTGGATAA CTCAAGCTAA CCAAAATCAT CCCAAACTTC CCACCCCATA CCCTATTACC ACTGCCAATT ACCTAGTGGT TTCATTTACT CTAAACCTGT GATTCCTCTG AATTATTTTC ATTTTAAAGA AATTGTATTT GTTAAATATG TACTACAAAC TTAGTAGTTT TTAAAGAAAT TGTATTTGTT AAATATGTAC TACAAACTTA GTAGT
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