pFUSE-mIgG2A-Fc2
$128.00
In stock
SKU
PL77157
100μg liquid plasmid(Instantly):$149
| name | pFUSE-mIgG2A-Fc2 | another name | apFUSE-mIgG2A-Fc2 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Antibody expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACCTGAGA TCACCGGCGA AGGAGGGCCA CCATGTACAG GATGCAACTC CTGTCTTGCA TTGCACTAAG TCTTGCACTT GTCACGAATT CGATATCGGC CATGGTTAGA TCTCCCAGAG GGCCCACAAT CAAGCCCTGT CCTCCATGCA AATGCCCAGC ACCTAACCTC TTGGGTGGAC CATCCGTCTT CATCTTCCCT CCAAAGATCA AGGATGTACT CATGATCTCC CTGAGCCCCA TAGTCACATG TGTGGTGGTG GATGTGAGCG AGGATGACCC AGATGTCCAG ATCAGCTGGT TTGTGAACAA CGTGGAAGTA CACACAGCTC AGACACAAAC CCATAGAGAG GATTACAACA GTACTCTCCG GGTGGTCAGT GCCCTCCCCA TCCAGCACCA GGACTGGATG AGTGGCAAGG AGTTCAAATG CAAGGTCAAC AACAAAGACC TCCCAGCGCC CATCGAGAGA ACCATCTCAA AACCCAAAGG GTCAGTAAGA GCTCCACAGG TATATGTCTT GCCTCCACCA GAAGAAGAGA TGACTAAGAA ACAGGTCACT CTGACCTGCA TGGTCACAGA CTTCATGCCT GAAGACATTT ACGTGGAGTG GACCAACAAC GGGAAAACAG AGCTAAACTA CAAGAACACT GAACCAGTCC TGGACTCTGA TGGTTCTTAC TTCATGTACA GCAAGCTGAG AGTGGAAAAG AAGAACTGGG TGGAAAGAAA TAGCTACTCC TGTTCAGTGG TCCACGAGGG TCTGCACAAT CACCACACGA CTAAGAGCTT CTCCCGGACT CCGGGTAAAT GAGCTCAGCT AGCTGGCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGAATTAATT CTAAAATACA GCATAGCAAA ACTTTAACCT CCAAATCAAG CCTCTACTTG AATCCTTTTC TGAGGGATGA ATAAGGCATA GGCATCAGGG GCTGTTGCCA ATGTGCATTA GCTGTTTGCA GCCTCACCTT CTTTCATGGA GTTTAAGATA TAGTGTATTT TCCCAAGGTT TGAACTAGCT CTTCATTTCT TTATGTTTTA AATGCACTGA CCTCCCACAT TCCCTTTTTA GTAAAATATT CAGAAATAAT TTAAATACAT CATTGCAATG AAAATAAATG TTTTTTATTA GGCAGAATCC AGATGCTCAA GGCCCTTCAT AATATCCCCC AGTTTAGTAG TTGGACTTAG GGAACAAAGG AACCTTTAAT AGAAATTGGA CAGCAAGAAA GCGAGCTTCT AGCTTATCCT CAGTCCTGCT CCTCTGCCAC AAAGTGCACG CAGTTGCCGG CCGGGTCGCG CAGGGCGAAC TCCCGCCCCC ACGGCTGCTC GCCGATCTCG GTCATGGCCG GCCCGGAGGC GTCCCGGAAG TTCGTGGACA CGACCTCCGA CCACTCGGCG TACAGCTCGT CCAGGCCGCG CACCCACACC CAGGCCAGGG TGTTGTCCGG CACCACCTGG TCCTGGACCG CGCTGATGAA CAGGGTCACG TCGTCCCGGA CCACACCGGC GAAGTCGTCC TCCACGAAGT CCCGGGAGAA CCCGAGCCGG TCGGTCCAGA ACTCGACCGC TCCGGCGACG TCGCGCGCGG TGAGCACCGG AACGGCACTG GTCAACTTGG CCATGATGGC TCCTCCTGTC AGGAGAGGAA AGAGAAGAAG GTTAGTACAA TTGCTATAGT GAGTTGTATT ATACTATGCA GATATACTAT GCCAATGATT AATTGTCAAA CTAGGGCTGC AGGGTTCATA GTGCCACTTT TCCTGCACTG CCCCATCTCC TGCCCACCCT TTCCCAGGCA TAGACAGTCA GTGACTTACC AAACTCACAG GAGGGAGAAG GCAGAAGCTT GAGACAGACC CGCGGGACCG CCGAACTGCG AGGGGACGTG GCTAGGGCGG CTTCTTTTAT GGTGCGCCGG CCCTCGGAGG CAGGGCGCTC GGGGAGGCCT AGCGGCCAAT CTGCGGTGGC AGGAGGCGGG GCCGAAGGCC GTGCCTGACC AATCCGGAGC ACATAGGAGT CTCAGCCCCC CGCCCCAAAG CAAGGGGAAG TCACGCGCCT GTAGCGCCAG CGTGTTGTGA AATGGGGGCT TGGGGGGGTT GGGGCCCTGA CTAGTCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG CATCATCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG TTATGTAACG CCTGCAGGTT AATTAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGGCTAGTT AATTAACATT TAAATCAGCG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGT AACTAACATA CGCTCTCCAT CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT TTCTCTATCG AA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
