pIZT-V5-His
$128.00
In stock
SKU
PL77040
pIZT/V5-His
100μg liquid plasmid(Instantly):$149
| name | pIZT-V5-His | another name | apIZT-V5-His |
| Insert Size (bp) | Plasmid host | Insect cells | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | Green |
| Promoter | OpIE-2 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pCMV-R：TCCAAACTCATCAATGTATC | |
| 3′ sequencing primer | Forward(left ITR)：CCTCTGACTTGAGCGTCGAT | Copy number | middle |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCATGAT GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC TGTGTTTTCA TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT TTTTGCACTG CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG TTTCGTAGAC TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC ACACATTGAA CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT TATCGGGTCG CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA TCGTGACAGG ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG AACAGGACGC GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC GCCCGTCCCG CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA ACAGCATCTG TTCGAATTTA AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGCGGTTCG AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAT CTGACTAAAT CTTAGTTTGT ATTGTCATGT TTTAATACAA TATGTTATGT TTAAATATGT TTTTAATAAA TTTTATAAAA TAATTTCAAC TTTTATTGTA ACAACATTGT CCATTTACAC ACTCCTTTCA AGCGCGTGGG ATCGATGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC GAAACACGCA CGGCGCGCGC ACGCAGCTTA GCACAAACGC GTCGTTGCAC GCGCCCACCG CTAACCGCAG GCCAATCGGT CGGCCGGCCT CATATCCGCT CACCAGCCGC GTCCTATCGG GCGCGGCTTC CGCGCCCATT TTGAATAAAT AAACGATAAC GCCGTTGGTG GCGTGAGGCA TGTAAAAGGT TACATCATTA TCTTGTTCGC CATCCGGTTG GTATAAATAG ACGTTCATGT TGGTTTTTGT TTCAGTTGCA AGTTGGCTGC GGCGCGCGCA GCACCTTTGC CGGGATCTGC CGGGCTGCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCGGTA GAAAAAATGG CTAGCAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCT ACATACGGAA AGCTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT TTTCCCGTTA TCCGGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GAACTACAAG ACGCGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTC GGACACAAAC TCGAGTACAA CTATAACTCA CACAATGTAT ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCAAAATT CGTCACAACA TTGAAGATGG ATCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCGACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GCGTGACCAC ATGGTCCTTC TTGAGTTTGT AACTGCTGCT GGGATTACAC ATGGCATGGA TGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACCGACGC CGACCAACAC CGCCGGTCCG ACGCGGCCCG ACGGGTCCGA GGGGGGTCGA CCTCGAAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCT
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